【Objective】 To analyze the relevant factors affecting the positive psychological capital and communication ability of phlebotomist in blood stations based on the theory of positive psychology and communication behavior, so as to provide reference for the development of professional nursing work in blood stations. 【Methods】 According to the Extensive Positive Psychological Capital Questionnaire and Zeng Kai′s Blood Donor Nurses Communication Ability Evaluation Scale, an online survey was conducted by WJX (a platform providing functions equivalent to Amazon Mechanical Turk) and statistical analysis was performed using SPSS25.0. 【Results】 The average score of positive psychological capital among phlebotomists was 4.78±0.75, with four dimensions (from high to low) as optimism 5.23±1.09, hope 4.9±0.92, self-efficacy 4.57±0.88 and resilience 4.52±0.69 (P<0.05). The highest and lowest scores among the six dimensions were communication ability and emotional support ability, as 4.55±0.58 and 4.35±0.66, respectively (P>0.05). There was a positive correlation between positive psychological capital and communication ability of phlebotomists(P<0.01). The academic qualifications, professional titles, training frequency and knowledge of self-perception are the factors affecting the positive psychological capital and communication ability of phlebotomists.. 【Conclusion】 Managers in blood collection and supply institutions should encourge phlebotomists to study, think and practice initiatively, and conduct education and training on psychological and professional quality of phlebotomists continuously.