Objective To introduce the advance of the diagnosis and tretment of breast cancer(BC). Methods Reveiwing the recently world reference regarding to the diagnosis and treatment of BC was done for making an advancing paper of BC. Results The NF ?B and telomerase may be a new target of anti cancer of breast. The ECT, fiberoptic mammaroductoscopy, examine of micrometastase of BC, immuno cell chemical examine were progressing in the diagnosis of BC. The advance of the treatiment of BC included breast conservative surgery, lymphatic mapping and sentinel node biopsy, biochemical response modifier, new adjuvant chemotherapy, TAM and 1,25 (OH) 2D 3 treatmen. Conclusions The idea and mould of the diagnosis and treatment of BC have advanced obviously.

Sera from 80 patients suffering from gastritis and/or digestive ulcer were examined for IgG, IgA and IgM to Helicobacter pylori by enzyme-linked immunosorbent assay (ELISA). Anti-H. pylori IgG and IgA in serum could be used for diagnosis of H. pylori infection, but the former had good sensitivity (92.0%) and the latter had a great specificity (94.4%). Serum anti-H, pylori IgM level was not associated with H. pylori infection. There were no remarkable relationship between H. pylori infection degree arid levels of anti-H, pylori IgG IgA. The positive percentage of anti-H, pylori IgG of health population deceased after 50 years old but the anti-H, pylori IgA increased.

Multiple chromosomal aberrations, nucleotide substitutions, and epigenetic modifications may occur in human cancer cells, which drive malignant transformation. The Cancer Genome Atlas (TCGA) project aims to promote large-scale multi-dimensional analysis of these molecular characteristics in human cancer and rapidly provide data to researchers. In this study, we introduce four flow paths of the production of TCGA data, the collections of various cancer types, the data category and level, and the standardized pipeline of data analysis, as well as several existing data analytical tools. We used ovarian cancer as an example to introduce the application of the TCGA data in the analyses of mutation, copy number, analysis, and expression. We summarized the important findings of glioblasto-ma by TCGA teams.

Objective To study the apoptotic induction effect of Thapsigargin on estrogen receptor positive human breast cancer cell lines MCF 7. Methods Cells were treated with Thapsigargin and 5 FU in vitro . The rate of cell apoptosis and distribution of cell cycle were detected on flow cytometry. The cell viability was measured by MTT assay and ultrastructural changes in apoptotic cells were confirmed by transmission electron microscopy.Results Thapsigargin could increase the rates both of cell apoptosis and growth supression of MCF 7 cells induced by 5 FU and alter the distribution of cell cycle. Under electron microscope, apoptotic bodies in MCF 7 cells considerably increased.Conclusion Thapsigargin apparently enhances the effect of apoptotic induction of 5 FU on MCF 7 cells, it is worthy of being further studied.

Objective To investigate the diagnosis and treatment of primary retroperitoneal tumor,and improve the early correct diagnostic rate and the resection rate.Method 86 patients with primary retroperitoneal tumor(PRT) were analyzed by review of the clinical materials from 1990 to 2000.Results Abdominal or waist pain was the main symptom,and abdominal or waist mass was the main sign.52.6% percent of the patients got correct diagnosis before operation.62 cases were followed up,the 2-year survival rate for complete resection cases were 61.3%,while the patients who received palliative resection or biopsy died within one year.The resection rate of tumors was 81.3% in benign tumors and 37.7% in malignant tumors,the recurrent rates after complete resection were 15.4%,60%,respectively.Conclusions The earlier diagnosis,totally removal of tumors at first time,periodic examination after operation are three important ways.The operative treatment is very imperative for the patients with recurrence.

Objective To review the mechanisms of breast cancer escaping from host immune surveillance. Methods The current literatures on the mechanisms of breast cancer cells escaping from host immune surveillance were reviewed in the following aspects: alterations of MHC-Ⅰmolecule phenotype, deficiency of costimulatory molecules, apoptosis of T-lymphocytes induced by breast cancer cells presenting Fas ligand (FasL) and TNF-related apoptosis-inducing ligand (TRAIL) and host immune tolerance induced by tumor cells. Results Loss of classical antigen-presenting human leukocyte antigen (HLA) class-Ⅰmolecules, expression of non-classical HLA class-ⅠmoleculesHLA-G, loss of costimulatory molecule B7, apoptosis of T-lymphocytes induced by tumor cells presenting FasL and TRAIL and antigen-inducing host immune tolerance were related to breast cancer escaping from immune surveillance. Conclusion Breast cancer cells escape from host immune surveillance by altering MHC-Ⅰmolecules, lacking of costimulatory molecules, inducing of apoptosis of T cells and host immune tolerance. But the factors resulting in breast cancer escaping from host immune surveillance are not yet clear and should be further studied.

Objective To investigate the effects of CD151 on the expression of phosphoinositide 3-kinase(PI3K) and the proliferation of human umbilical vein endothelial cells(HUVEC) in vitro.Methods The plasmid p-AAV-CD151 was constructed and transfected into HUVEC.The proliferation activity of HUVEC was measured by MTT colorimetric assay.The expressions of CD151 and PI3K were tested by Western Blot.Results After transfection with p-AAV-CD151,the expressions of CD151 and PI3K were significantly increased compared with those of non-transfected group and p-AAV-GFP transfected group(P

Objective To study the value of p27 and Ki-67 detection in differentiating benign from malignant thyroid follicular neoplasms. Methods Labelling index (LI) of thyroid neoplasms from thyroidectomy specimens were detected immunohistochemically Results The normal thyroid group had the highest expression of p27 , and the lowest expression of Ki-67. There was significant difference in the expressions of p27 and Ki-67 between follicular thyroid adenomas (FA) and follicular thyroid cancers (FTC) (P

Objectiver　 To establish murine breast precancerous model and evaluate the effect of exogenous estrogen and tamoxifen.　 Method　 50 female Wistar rats were divided into contol group (n＝12), the other 38 received low dose carcinogen (DMBA) by gastrogavage, and then divided into DMBA group (common diet,n＝8); DMBA+DES group(DES added chow,n＝15); and DMBA+TAM group (TAM added chow,n＝15). 3 months later, the status of breast hyperplasia, AgNOR and DNA content were measured. 　Results　 All indexs in control group and DMBA+TAM group were normal.However,in DMBA group and DMBA+DES group,the breast hyperplasia rate was 75%, 93.3%, AgNOR was 3.71±0.76, 5.60±0.42, DNA was 88.59±7.17, 137.75±3.13 respectively.Compared with control group and DMBA+TAM group, the differences of indexes in DMBA and in DMBA+DES group were significant (P<0.01). 　Conclusion　 Low dosage DMBA administered by gastrogavage established breast precancerous model. Exogenous estrogen promotes the development of breast precancerous disease induced by DMBA, while TAM suppresses the canceration.

Aim To study the effects of pyrrolidine dithiocarbamate(PDTC) on matrix metalloproteinase-9(MMP-9) expression in alveolar macrophages(AM) induced by TNF-?,and then to explore it's signal pathway.Methods AM were collected from bronchoalveolar lavage fluid in patients with chronic obstructive pulmonary disease.AM were pretreated with PDTC and then stimulated by TNF-? or IL-1.MMP-9 mRNA expression was detected by semi-quantitative reverse transcription-polymerase chain reaction and MMP-9 protein expression was detected by Western blot.Phospho-I?B? protein levels induced by TNF-? or IL-1 were detected by Western blot.NF-?B activity was detected by electrophoretic mobility shift assay.Results Both the mRNA and protein levels of MMP-9 induced by TNF-? in AM were significantly elevated in a dose dependent manner(P0.05).PDTC had distinct effect of inhibiting the activation of NF-?B induced by TNF-? or IL-1 in a dose-dependent manner(P0.05).Conclusions NF-?B plays an important role in MMP-9 expression induced by TNF-? in AM;PDTC can down-regulate MMP-9 expression induced by TNF? in AM possibly through preventing the degradation of I?B? via ubiquitylation-proteasome proteolytic pathway.