An investigation of palmar patterns on 900 cases Han nationality of China wasmade.The main purpose of this paper is to provide the normal values of Chinese dermatoglyphics and human genetics.The main characters of palmar patterns of Han nationality are as follows:mainline A:type Ⅰ 7.89%,type Ⅲ 91%,type Ⅴ 1.11%;main line B:type Ⅴ 80.72%,type Ⅶ 16.78%,type Ⅸ0.67%,type Ⅺ0.06%,type O(absent)0.11%,typeⅢ 1.67%;main line C:type Ⅴ 38.78%,type Ⅶ 32.67%,type Ⅸ 16.06%,typeⅪ0.44%,type O(absent)12.06%;main line D:type Ⅶ and type Ⅸ all 40.61%,type Ⅺ 18.39%,type O(absent)0.39%.The average value of AD main line formula is 6.50?1.61(SD).There are sig-nificant difference between sexes and two hands(R.L.)(P0.05).The frequency of complicatedpalmar creases is 5.22%.The rate of symmetrical main lines between two hands is34.89%.The main type of A.B.C.D main line is 3.5′.5″.7(18.11%).

Objective To study the chemical constituents of Phlomis medicinalis.Methods The constituents were isolated and purified by various modern chromatography techniques and the structures were elucidated by physicochemical properties and spectral analyses.Results Ten compounds were obtained and elucidated as 1-dehydroxyshanzhigenin methyl ester(Ⅰ),notohamosin A(Ⅱ),verbascoside(Ⅲ),3,4-dihydroxyphenyl ethanol(Ⅳ),succinic acid(Ⅴ),sucrose(Ⅵ),2,6-difructose(Ⅶ),butyl-?-D-fructopyanoside(Ⅷ),D-glucose(Ⅸ),and D-fructose(Ⅹ).Conclusion All the compounds are isolated from this plant for the first time,compound Ⅱ is obtained from the plants of Phlomis Linn.for the first time,and compound Ⅰ is a new one.

Objective: To study the difference between the facial nerve injury by gun-shot and that by blast. Methods: 40 adult dogs were divided into 2 groups, with 20 dogs in each. In the blast group, the impact wave was simulated by detonator blast in the distance of 10 cm over the dog's masseter. At the same time,a steel ball was projected to the dog's face by a rifle to imitate segments in blast. In the gun-shot group, only the steel ball was used. Then the facial nerve was taken at the time of immediateness, 6 hours, 1 day, 3 days and 7 days following the injury for HE or immunocytochemical staining. And the facial nerve conduction velocity was examined 6 hours, 3 days and 7 days after injury.Results: The facial nerve edema was found in both groups just after injury. There was scattering hemorrhage and little hematoma below the spineurium in the gun-shot wound, as well as the diffusing bleeding and larger hematoma in blast wound. In the blast group, the severe edema could be found by light microscope in the whole extracranial facial nerve. Axonal fragments, infiltrating imflammatory cells and few neurofilament protein NF positive axons were observed. In the gun-shot group, only scattering damage was found within 2 cm from the wound track.Conclusion: The facial nerve injury by gun-shot bacomes less severe with the increase of distance from the wound track, but the blast injury is wider and more severe.

Objective:To analyse the effects of mandibular swing approach in the treatment of the tumors in infratemporal fossa. Methods:11 patients with tumors in infratemporal fossa treated by the surgical operation with mandibular swing approach. The mandi-ble cut was at the front of foramina mentale in 5 cases, at the front of mandible angle in 4 cases and at the middle of chin in 2 cases re-spectively. Results:According to the nature, location, size and the relationship of the tumors with peripheral nerve and blood vessels, flexible selection of the position of mandibular cut could fully expose and completely remove the infratemporal fossa tumor. Conclusion:Surgical treatment of the tumors in infratemporal fossa by mandibular swing approach is safe and effective.

Objective To investigate the effects of gene transfection with human growth/differentiation factor 5(GDF5)on the growth and difierentiation of bone nlarrow stromal stem cells (BMSCs).Methods GDF5 gene was trans fected into BMSCs by liposome method. Then cell proliferation and cycles were examined by MTT and flow cytometry respectively. Cell morphology was observed under light microscope and electron microscope (EM).GDF5 and Collagen Ⅱ were detected at the level of mRNA and protein by RT-PCR and immunocytochemistry. Alkaline phosphate activity was examined by lead citrate method. Osteocalcin mRNA expression was determined bv RT-PCR. ResulIs GDF5 gene was transfected into BMSCs successfully and the transfected cells still maintained their natural growth and proliferation features. Stable expression of GDF5 gene in BMSCs was obtained. The trans fected ceils had basically the same proliferation ability and cell cycles as the untransfccted ones. After transfection comparatively more polygonal cells could be seen in light microscope, showing irregular arrangement mode. Plenty organells were observed and cell nucleus showed irregular shape under EM. The expressions of Collagen Ⅱ mRNA and protein were positive. But osteocalcin mRNA expression was negative. Conclusion Since BMSCs can be induced by GDF5 to differentiate into chondrogenic cells. GDF5 gene-modified BMSCs may be used as candidate seed cells of cartilage tissue engineering.

Objective To determine ecdysterone content in Radix Achyranthis Bidentalae by near-infrared diffuse reflectance spectroscopy (NIRDRS) combined with partial least squares (PLS). Methods NIRDRS and PLS. Results The correlation coefficient of the quantitative mathematics model between the prediction and the true values was 0.948 9. It is feasible to appling the above established method to determination of chemical constituents in Chinese materia medica. Conclusion Compared with other methods, the method is simple, rapid, high efficient, low expenditure, no pollution and with preparation undone and is suit for large quantity of unknown samples.

Objective To study the expression of eukaryotic translation initiation factor 4E(eIF4E)in the pathological scars and its probable role in the pathogenesis of pathological scars.Methods Immunohistochemiscal technique was performed to detect the expression and distribution of eIF4E protein in hypertrophic scars(14 cases),keloids(25 cases),mature scars(20 cases)and normal skins(20 cases).Reverse transcription polymerase chain reaction(RT-PCR)was used to detect the eIF4E mRNA level in hypertrophic scars(7 cases),keloids(8 cases),mature scars(8 cases)and normal skins(8 cases).Results Thepositive rate of eIF4E protein expression was remarkably significant difference between normal scars and pathological scars(P＜0.05).The level of eIF4E mRNA in pathological scars 1.73±0.31was higher than that in control group 0.99±0.28.There was significant difference between two groups (P＜O.05).Conclusions The expression of eIF4E is increased in pathological scar.eIF4 E expression is closely associated with the development of pathological scar.Therefore,eIF4E overexpression may play an important role in the proliferation of fibroblasts and in the pathogenesis of pathological scar.

Electrical instability easily induces a unidirectional conduction block, resulting in ventricular tachycardia (VT) or even fibrillation (VF). Cardiac memory affects dynamic electrical characteristics through previous pacing so that it makes the memory important in arrhythmia study. This paper investigates the impact of the rapid pacing duration on cellular excitability and its mechanism. Based on the canine endocardial single cell, a one-dimensional tissue model was developed. Simulations were realized with OpenMP parallel programming method. The results showed that with repetitive pacing, the cellular excitability became low while the conduction velocity decreased. Accumulation of intracellular [Ca2+]i and [Na+]i and depletion of [K+]i led to the shift of membrane current-voltage curves, changing the membrane resistance. Excitability determined by the resistance at the large width of stimulus pulse, therefore, it suggested that [Ca2+]i and [K+]i-induced memory formed the ionic substrates for the alteration of excitability.
Action Potentials ; Animals ; Computer Simulation ; Dogs ; Electric Stimulation ; Electrocardiography ; Heart Conduction System ; physiopathology ; Myocardial Contraction ; physiology ; Myocytes, Cardiac ; physiology ; Refractory Period, Electrophysiological ; physiology ; Tachycardia, Ventricular ; etiology ; physiopathology ; Ventricular Fibrillation ; etiology ; physiopathology

A flow controlling system for pulsed inhaled nitric oxide has been developed and tested, and here its features and initial animal experiments and clinical applications are described. The physical characteristic test indicates that the practical released dose of NO gas is very close to the theoretical flow of NO gas at variant pressures. Animal experiments demonstrate that inhaled NO gas concentration is lower than the concentration of theoretical inhalation, but the variance is not remarkable (p>0.05). When sixteen cases with CHD and PH were chosen to inhale NO gas (15 ppm, 15 min) PAP and PVR of all cases were reduced after inhalation of NO gas from 617 +/-51.3 dyn x s x cm(-5), 54.4+/-13.1 mmHg to 417+/-36.9 dym x s x cm(-5), 33.8+/-12.3 mmHg (PVR, p<0.01; PAP, p<0.01) respectively. When gas inhalation was stopped, these values returned to their base lines after a short period of time. All these show that the pulsed inhaled NO flow controlling instrument in accordance with the requirements of the designing, can be widely used in clinical diagnoses and treatments and will be a new tool offered for the treatments of the patients with PH.
Administration, Inhalation ; Animals ; Blood Pressure ; Cardiac Output ; Child ; Equipment Design ; instrumentation ; Female ; Heart Defects, Congenital ; physiopathology ; therapy ; Humans ; Hypertension, Pulmonary ; physiopathology ; therapy ; Male ; Nebulizers and Vaporizers ; Nitric Oxide ; administration & dosage ; analysis ; Rabbits ; Vascular Resistance