Objective To investigate the sedation values of dexmedetomidine combined with midazolam in appendectomy.Methods 90 patients with acute appendicitis were studied prospectively.According to the principle of completely random digital table,all patients were randomly signed into the dexmedetomidine combined with midazolam group,single midazolam group or single dexmedetomidine group.The hemodynamic changes in the three groups were compared.Results There were no significant differences in the three groups in heart rate and mean arterial pressure at t0 (P >0.05).When compared with the single midazolam group or dexmedetomidine group,patients in dexmedeto-midine group got a significant reduction in heart rate at t1 [(79.73 ±10.36)beat/min vs.(93.30 ±19.32)beat/min and (90.85 ±18.23 )beat/min,F =5.796,P =0.004];at t3 [(79.51 ±11.21 )beat/min vs.(87.53 ± 18.35)beat/min and (95.03 ±14.64)beat/min,F =8.006,P =0.001];and at t5 [(79.73 ±12.24)beat/min vs. (91.80 ±17.71)beat/min and (89.62 ±14.26)beat/min,F =5.584,P =0.005];a reduction in heart rate at t2 and t4,though there was no statistical difference (P >0.05);a significant reduction in mean arterial pressure at t1, t2,t3,t4 and t5 (F =8.444,6.591,8.165,17.121 and 6.977,P =0.000,0.002,0.001,0.000 and 6.977);a sig-nificant reduction in visual analogue scales [(3.40 ±1.28 )points vs.(4.50 ±1.81 )points and (4.37 ± 1.71)points,F =4.131,P <0.05 ];a significant reduction in operation duration [(111.17 ±15.77 )min vs. (129.33 ±13.98)min and (132.50 ±14.67)min,F =0.000,P <0.05];and a significant reduction in bleeding vol-ume [(202.47 ±61.07 )mL vs.(246.87 ±52.06 )mL and (241.93 ±58.71 )mL,F =0.006,P <0.05 ]. Conclusion Dexmedetomidine combined with midazolam contributed to maintaining the hemodynamic stable and reducing the operation duration,intra -operation bleeding volume and visual analogue scales.

The arteries of the palmar part of the hand were examined in 50 hands of adult under the operating microscope.The arteries of the hand are arranged at three main levels: the superficial palmar arteries, the deep palmar arteries and the dorsal network. The arterial anastomoses between the levels may be divided into 2 groups: the marginal and the central anastomoses, the former which is situated at the radial and ulnar borders of the palm can be subdivided into superficial and deep groups, the latter is located in the central part of the palm including the direct and indirect anastomostic branches, the proximal and distal perforating branches, and the intermetacarpal perforating branches. The deep arch and its branches, as the center in the total arterial architecture of the palmar part of the hand, closely links with the arteries of the superficial and dorsal ones, playing an important role in regulating the collateral circulation of the hand.The flow formula of the Poiseuille's law indicates that the significant correlation exists between the arterial diameter and the blood flow. For the variety of diameter of each portion of the anastomostic branches, we inferred that the main source of blood flow in the superficial arteries is the ulnar artery and in the deep is the radial artery. The blood within the marginal anastomoses flows from palmar to dorsal, the central mainly from the deep arch and its branches.The dominant parts of the collateral circulation of the hand were found, that is, the deep arch dominantes over the superficial arch and the dorsal network, the central anastomosis over the marginal, the deep branches of the marginal over the superficial branches.The arrangement of the digital arteries has been discussed.

The construction of on campus practice base is crucial for improving the quality of practical teaching.We put forward a construction pattern of‘grid type' pharmacy teaching comprehensive laboratory.It covers three-leveled experimental platform and professional teaching laboratory according to the research and development process of new drug.It also forms the communication network between learning and working demand of students.This provides new ideas for the construction of pharmacy teaching laboratory in newly-built university.

AIM:To investigate the inhibitory effect of dihydro-?-erythroidine on nicotine induced-current in cultured superior cervical ganglion of neonatal rats.METHODS:Pneumatic pressure administration of drug and whole-cell recording techniques were performed to compare induced-current amplitude.RESULTS:Dihydro-?-erythroidine competitively antagonized nicotinic effect,and EC50 was about 0.015mmol/L.CONCLUSION:Dihydro-?-erythroidine is a competitive antagonist of nicotinic receptors in sympathetic neurons.

Chronic obstructive pulmonary (COPD) has significant extra pulmonary effects, which could induce atrophy of peripheral skeletal muscle and respiratory muscles, and significantly influence the respiratory function and quality of life. Muscle nuclear factor (NF)-κB activation plays a key role in the skeletal muscle atrophy. This article discussed the potential mechanisms on how NF-κB signaling pathway increasing protein breakdown and reducing muscle regeneration. On this basis, we explored the role of NF-κB in skeletal muscle at-rophy in patients with COPD during exercise.

Objective To investigate the effect of recombinant human growth hormone (rhGH) action on pregnancy rate of polycystic ovarian syndrome(PCOS).Methods The PCOS who were client in our hospital in February-August,2012 were investigated.The rhGH group (41 cases) were that all patients took rhGH at the same time ovulation.The control group (41 cases) were no medication group.The two groups had sex action after ovulation.Ovulation rate,pregnancy rate and endometrial score of the two groups were compared.Results Ovulation rate of rhGH group was 90.2％ (37/41),the control group was 68.2％ (28/41),the difference was statistically significant (x2 =3.459,P =0.036).Clinical pregnancy rate of RhGH group was 32.7％ (12/37),the control group was 25.0％ (7/28).The difference was statistics significance (x2 =4.319,P =0.032).Endometrial score of rhGH group was (14.96 ± 2.18),the control group was (12.77 ± 2.60) points,the difference between groups was statistically significant (t =-3.356,P =0.032).Conclusion Giving rhGH ovulation cycle specification can promote ovulation PCOS patients and improve the adverse factors affecting pregnancy,improving the pregnancy rate.

Objective To explore the effect of purification on monoclonal antibody (MAb) against PGRP by Protein A-Sepharose affinity chromatography, and to provide some based data for the purification of other antibody using the same method. Methods The ascites which include MAb was purified by Protein A-Sepharose affinity chromatography. The purity and activity of MAb was tested by SDS-PAGE and ELISA. The biological function was identified by flow cytometer and immunohistochemistry. Results The average concentration of protein in ascites before purification is 23.62 mg/ml. Before and after purification, the total protein is 148.79 mg and 146.67 mg, respectively. The recovery coefficient of protein is 98.58%. The concentration of MAb in ascites is 5.21 mg/ml averagely. The MAb purity is more than 95 %. The immunoactivity of purified antibody is higher than that of unpurified antibody. Conclusion The purity of MAb against PGRP purified by Protein A-Sepharose affinity chromatography is very high. The immunoactivity of purified antibody is higher than that of unpurified antibody. So the ProteinA-Sepharose affinity chromatography is a rapid, convenient and reliable method for the purification of MAb Against PGRP.

Objective To study expression and activation of p38 mitogen activated protein kinase (MAPK) in vascular endothelial cells dysfunction in preeclampsia. Methods From Sept. 2009 to Mar.2010, 54 pregnant women underwent deliveries in the First Affiliated Hospital of Chongqing Medical University were enrolled in this study, including 20 patients in mild preeclampsia group, 16 patients in severe preeclampsia group and 18 women with term cesarean section without perinatal complications as control group. Placental endothelial cells were labeled by CD34 to assay microvessel density (MVD) of each group. Immunohistochemical SP and western blot were used to detect localization and expression of p-p38 MAPK protein, respectively. The levels of sera soluble fms-like tyrosine kinase-1 (sFlt-1)and soluble endoglin(sEng) were measured by ELISA. Results ①The MVD of placenta were 103 ± 3 in control group, 81 ±5 in mild preeclampsia group and 63±4 in severe group, respectively, which showed statistical difference among each group (P＜0.05).②The expression of p38 MAPK protein were 0.84±0.05 in control group,0.90±0.14 in mild group and 0. 86 ±0.18 in severe group, which did not reach remarkable difference among each group (P＞0.05). The expression of p-p38 MAPK protein were 0.13±0.05 in control group,0.59±0.12 in mild group and 1.16±0.18 in severe group, which show statistical difference among each group(P＜0.05).(3) The localization of p-p38 was in trophoblast, endothelial cells and a few (5.2±0.3)and(10.9±0.4)μg/L in control group,(12.5±1.2) and (20.4±5.3)μg/L in mild group and (19.3±3.0) and (29. 5 ±3.7) μg/L in severe group. When drawing paired comparison in those p-p38 MAPK protein levels and the concentrations of serum sFlt-1, sEng in preeclampsia groups (r=0.68,P＜0.05;r=0.87,P＜0.05). Conclusions The remarkable activation of the p38 MAPK in the placenta of patients with preeclampsia induced the increased levels of sFlt-1 and sEng in maternal serum, which confer the injury of vascular endothelial cells that caused the significant decline of MVD in placentas. p38 MAPK signaling might be one of the key pathways in vascular endothelial cell dysfunction in preeclampsia.

Objective To compare the mid- and long-term results of tricuspid valve (TV) repair with or without an annuloplasty ring. Methods Two hundred seventy-seven patients underwent TV repair at our division (Sep. 2001 to Sep. 2008) ,of which 203 had, predominantly, a De Vega or Kay procedure (non-ring group) and 74had an annuloplasty with an artificial ring (ring group). TV pathology mainly was functional (secondary) and several with rheumatic leaflets involvement. Concomitant procedures consisted of mitral valve surgery in all patients,aortic valve surgery in 81 ,and coronary bypass in 19. Clinical and echocardiographic data followed for 1.5 to 3.5years were obtained. Results Postoperationally,the mid-term(1.5 years) follow-up was 100% completed and the long-term follow-up for 3. 5 years was 89. 9%. The recurrence of TV regurgitation (TR) of moderate and lower degree was not significantly different(χ2 = 1.3128, P= 0.26) in the 1.5 years follow-up between the two group,whereas the recurrence of TR of moderate to severity degree was significantly less in the ring group (χ2 =5. 8159,P =0.023).In the long term follow up,the TR in the ring group (25%) was significantly lower than that of 15% in the non-ring group (χ2 = 4. 9328, P = 0.036) . There are higher proportion of patients developing to moderate TR in the non ring group(34%) than in the ring group (10%) (χ2 =7. 9120,P =0.005). The TR developed fast in the ring group,increasing from 18% at 1.5 years follow up to 10% at 3.5 years follow up (χ2 = 2. 1327, P = 0.016),whereas it was relatively stable in the non-ring group,with 7% at 1.5 year follow up and 10% at 3.5 year follow up. Conclusions Placement of an annuloplasty ring in patients undergoing TV repair could remarkably improved the mid and long terum outcome. In clinic practice, we should be more positive to the functional TR and prefer to the procedure with annuloplasty ring.