OBJECTIVE:To provide reference for the further promotion ofNew Medical Reformand the further implementa-tion and improvement of National Essential Medicine System. METHODS:By systematic sampling,1728 outpatient prescriptions were randomly selected from a community health service center in Foshan during 2012 to 2015,and then analyzed and compared statistically in respects of the amount of drugs,rational drug use indexes,prescription cost,main disease composition,treatment cost. The change rules and characteristics of all the above mentioned information were all studied. RESULTS:The average number of medicines per prescription and the number of essential medicines decreased year by year,Comparied with 2012,the proportion of antimicrobial agents,the proportion of two or more antimicrobial agents,the proportion of injection prescription and the propor-tion of transfusion prescription were significantly reduced,the cost of medical treatment of patients decreased significantly;medi-cine cost and medicare reimbursement amount had increased to different extents,there was statistical significance(P<0.05). Multi-ple linear regression analysis(stepwise)showed that the cost of medical treatment was closely related to rationality of prescriptions. Respiratory diseases,cardiovascular and cerebrovascular diseases,gastrointestinal diseases were the top 3 of the major diseases, the proportion of prescriptions for respiratory diseases,gastrointestinal diseases decreased year by year,while that for cardiovascu-lar and cerebrovascular diseases increased year by year. CONCLUSIONS:The promotion ofNew Medical Reformand the imple-mentation of National Essential Medicine System play a positive role in promoting rational drug use and the quality of medical ser-vice in community health service center. However,there are still many problems in the community health service centers,such as too many medicines each prescription,the lack of diagnosis and treatment ability,the defects of medicine selection and administra-tion.

Objective To study the clinical effect of manipulation in the treatment of cervical vertigo due to atlantoaxial joint disorder. Methods A total of 86 patients in our hospital from year of July, 2010 to 2014, with cervical vertigo due to atlantoaxial joint disorder were divided into a control group and a treatment group randomly, with 43 patients in each group. The control group was treated by intravenous dripping of betahistine hydrochloride sodium chloride injection, while the treatment group was additionally treated by manipulation on the basis of the control group. The efficacy and symptoms changes together with average blood flow rate of LVA, RVA, BA, and the value of ADI, VBLADI, and DO were compared between the two groups after one week of the treatment. Results After the treatment, the improvement of vertigo and symptoms of the treatment group was between than the control group(t=12.655, P<0.05); the value of ADI and VBLADI decreased lower in the treatment group than the control group (t were 2.888 and 5.334 respectively, P<0.05);Vm of RVA, LVA and BA increased higher in the treatment group than the control group (t were 4.710, 3.534 and 5.335 respectively, P<0.01). Conclusions Manipulation combined with intravenous dripping of betahistine hydrochloride sodium chloride injection is effective in improving vertigo, decreasing ADI and VBLADI, and correcting atlantoaxial joint disorder.

Objective To establish human colon carcinoma LoVo/Adr cell line with multidrug resistance (MDR) and to study its MDR mechanism. Methods MDR cell line (LoVo/Adr) was induced by stepwise selection on exposure to increasing doses of adriamycin (ADR). The MDR of LoVo/Adr was detected by MTT assay and the distribution of its cell cycle was detected by flow cytometry. The expression of MDR related genes, including mdr1, MRP, GST ? and TopoⅡ was measured by RT PCR and the level of P gp was detected by immunohistochemistry. Results Compared with parental cells, the resistance line had a slower growth rate and longer doubling time. It was larger and mixed with giant cells in different sizes and the number of cells in S phase decreased while that in G1, G2 phase increased. The LoVo/Adr cell line showed 61 fold, 14 fold, 3 fold, 9 fold and 1 fold higher resistance to ADR, VCR, MMC, CTX and 5 FU respectively than its parental cell line. It was also cross resistant to VCR, MMC and CTX, but not to 5 FU. The parental LoVo cells showed no mdr1 expression and the level of mdr1 mRNA expression increased gradually according to the concentration of ADR in resistant cell lines, and the level of GST ? mRNA was only increased significantly in the induced initial stage, although the parental LoVo cells expressed a low level of GST ?. MRP mRNA expression was not detected in both parental cell line and resistant cell lines. The level of Topo ⅡmRNA remained stable. Conclusions LoVo/Adr cell line offers a model with a typical MDR phenotype for the study of MDR in human colon cancer. Its drug resistance was mediated by mdr1 and GST ?, not MRP and TopoⅡ.

To study adhesion of human bifidobacterial strains to cultured human intestinal epithelial cells in vitro mediated by purified adhesin. Results showed that the binding of human bifidobacterial strains to human intestinal epithelial cells appeared to be specific. The adhesion was time and dose-dependent.These data suggested adhesion of human bifidobacterial strains to cultured human intestinal epithelial cells in vitro can be mediated specifically by purified adhesin.

ObjectiveTo investigate the protection effect of bifidobacterial adhesin for intestine ischemia/reperfusion (I/R) injury on gut barrier function in rat.MethodsSeventy-two male SD rats were randomly divided into sham operation group (n =24), I/R model group (n =24) and pretreatment group of bifidobacterial adhesin (pretreatment group, n = 24).Six rats were anatomized at 6 h, 1 d, 4 d and 7d after inducing I/R model in each group, respectively.The pathological changes of the terminal ilea and the blood levels of TNFα, IL-6, IL-10, diamine oxidase (DAO), and the activity and content of D-lactic acid were observed.ResultsThe blood levels of TNFα, IL-6, DAO and D-lactic acid in I/R model group were significantly higher than sham operation group at all time points (P ＜0.05) , while the blood level of IL-10 was no significantly change.The activity of IL-6 and DAO in pretreatment group was significantly lower than I/R model group at all time points (P ＜ 0.05), the blood level of TNFαt in pretreatment group was significantly lower than I/R model group at 1 d, the blood level of D-lactic was significantly lower than I/R model group at 4 d and 7 d (P ＜ 0.05). Intestinal pathological damages were obviously milder in pretreatment group than I/R model group at all time points (Chiu's pathological scores: 6 h, 3.22 ±0.22 vs 3.57 ±0.20;1 d,3.77 ±0.13 vs 3.90 ±0.12;4 d,2.93 ±0.23 vs 3.07 ±0.21;7 d,2.10 ±0.30 vs 2.22 ±0.17,all P ＜ 0.05).ConclusionThe pretreatment of bifidobacterial adhesin could protect the intestinal mucosa from I/R injury, and alleviate intestinal ischemic reperfusion injury.