1.Survey of microorganism contamination of domestic environment and sanit ary status
Songzhe TANG ; Wei SHEN ; Zhenrong CAI ; Yinzhen LUO
Chinese Journal of Disease Control & Prevention 2001;5(1):23-24
Objective To explore the microorganism contaminat ion situation and disease incidence in family and requirements of disinfection. Methods The sanitation conditions in 212 families in Shanghai were investigated, and 200 of them were taken samples for microbiolo gical test. Re sults I n recent a year, members in 82.1% of families caught diseases caused by microorg anism infection. Only 18.4% of families usually used disinfectant, the disinfect ed object mostly was dishware. 22.8% of samples was contaminated seriously. Fungus was detected in 63.9% of samples. The average positive rate of HBsAg was 4.2%. Conclusions Domestic environment was potentially cont aminated by microorganism.
2.Safe use of glutaraldehyde in heath care institution
Wei SHEN ; Songzhe TANG ; Zhenrong CAI ; Yinzhen LUO
Chinese Journal of Disease Control & Prevention 2001;5(1):63-64
This article introduces a American Nati onal Standard-Safe use and han dling of glutaraldehyde-based products in health care institution. It pro vides g uidelines for the safe use and handling of glutaraldehyde as a disinfectant and sterilant in heath care institution by design consideration, proper work practi ces, information on properties and vapor monitoring of glutaraldehyde, and perso nnel qualifications, training, protective attire and health consideration.
3.Analysis of SRY gene in 8 cases of sex abnormality.
Hui, WANG ; Yun, TENG ; Hong, TIAN ; Yanping, TANG ; Yan, CHEN ; Zhenrong, YANG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2004;24(5):503-6
In order to investigate the relationship between sex dysplasia and sex-determining region Y (SRY) gene, 8 patients with sexual abnormality were analyzed by cytogenetic and molecular genetic methods. Fluorescence in situ hybridization (FISH) using PY3.4, X alpha satellite, and SRY probes was performed in each case to analyze the sex chromosome translocation and gene translocation. SRY gene was amplified by polymerase chain reaction (PCR) and its mutation was detected by direct sequencing. The results showed that among 8 patients, 5 were positive for SRY and the remaining negative for SRY. In the patients positive for SRY genes, 3 presented testes and the left 2 streak ovaries. In the patients negative for SRY, only one case presented testes, while 2 ovaries. Direct sequencing demonstrated that all SRY genes were normal in the patients positive for SRY genes. FISH technique demonstrated that SRY genes translocated from Ypter to Xpter in 2 46,XX phenotypic males positive for SRY genes. It was concluded that SRY gene is strongly involved in male sex determination, while a sequence of other genes may be taken into account in sexual development.
Genes, sry/*genetics
;
Gonadal Dysgenesis, 46,XX/genetics
;
Gonadal Dysgenesis, 46,XY/genetics
;
Sex Chromosome Disorders/*genetics
;
Sex-Determining Region Y Protein/*genetics
4.Analysis of SRY gene in 8 cases of sex abnormality.
Hui WANG ; Yun TENG ; Hong TIAN ; Yanping TANG ; Yan CHEN ; Zhenrong YANG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2004;24(5):503-506
In order to investigate the relationship between sex dysplasia and sex-determining region Y (SRY) gene, 8 patients with sexual abnormality were analyzed by cytogenetic and molecular genetic methods. Fluorescence in situ hybridization (FISH) using PY3.4, X alpha satellite, and SRY probes was performed in each case to analyze the sex chromosome translocation and gene translocation. SRY gene was amplified by polymerase chain reaction (PCR) and its mutation was detected by direct sequencing. The results showed that among 8 patients, 5 were positive for SRY and the remaining negative for SRY. In the patients positive for SRY genes, 3 presented testes and the left 2 streak ovaries. In the patients negative for SRY, only one case presented testes, while 2 ovaries. Direct sequencing demonstrated that all SRY genes were normal in the patients positive for SRY genes. FISH technique demonstrated that SRY genes translocated from Ypter to Xpter in 2 46,XX phenotypic males positive for SRY genes. It was concluded that SRY gene is strongly involved in male sex determination, while a sequence of other genes may be taken into account in sexual development.
Female
;
Genes, sry
;
genetics
;
Gonadal Dysgenesis, 46,XX
;
genetics
;
Gonadal Dysgenesis, 46,XY
;
genetics
;
Humans
;
Male
;
Sex Chromosome Disorders
;
genetics
;
Sex-Determining Region Y Protein
;
genetics
5.A new surgery treatment for thumb reconstruction by one-stage plasty free second toe transfer
Jingliang ZHANG ; Zhenrong XIE ; Junbo XIAO ; Yanwen LEI ; Jun SONG ; Mingfei HE ; Zhongming HUANG ; Hang LI ; Huanwei CHEN ; Qiaohong GUO ; Huancai TANG ; Chao LIU ; Keli LIU
Chinese Journal of Microsurgery 2008;31(5):335-337,403
Objective To investigate a more pedect method for a nice outward appearance of a reconstructed thumb.Methods A free one-stage plasty second toe transfer for thumb reconstruction by interchanging the whole skin-nail flap from the great toe with another one from the second toe.Results There were 12 cases in this group,following-up 6-9 months in 8 cases,7 cases was excellent and 1 cases was good.The reconstructed thumb got a nice looking and more normal function while no blight to the great toe occurred.Conclusion It is an effective new procedure in ameliorating outward appearance of the reconstructed thumb by transferring the free moulded second toe.
6.Therapeutic review of 23 subependymal giant-cell astrocytoma
Lijiang HAN ; Jizong ZHAO ; Zhenyu MA ; Song LIN ; Yuanli ZHAO ; Ge YUAN ; Nan JI ; Yongji TIAN ; Tong REN ; Dong ZHANG ; Zhenrong SUN ; Xinru XIAO ; Kai TANG ; Mingxue PIAO ; Xueji LI ; Hai LIU ; Xun YE ; Xiaolin CHEN
Chinese Journal of Postgraduates of Medicine 2009;32(23):18-21
Objective To analyze and estimate, the treatment of patients with histologically confirmed subependymal giant-cell astroeytoma (SEGCA). Methods The data from 23 patients with SEG-CA who were diagnosed between February 1995 and February 2008 were retrospectively evaluated. Various combinations of surgery and radiotherapy had been used for treatment. Results Total resection was 16 cases, subtotal resection was 7 cases, radiotherapy was 17 cases. The average follow-up time was 53 months.One postoperative SEGCA recurrence. Epilepsy was totally disappeared in 17.6% (3/17), partly disappeared in 47.1%(8/17). All cases survived. Conclusions The key of treatment is total resection. The significance of radiotherapy is not sure. The overall prognosis of SEGCA is favorable.
7.Central neurocytoma: analysis of 71 cases
Lijiang HAN ; Jizong ZHAO ; Shuo WANG ; Ge YUAN ; Nan JI ; Yongji TIAN ; Tong REN ; Dong ZHANG ; Zhenrong SUN ; Xinru XIAO ; Kai TANG ; Mingxue PIAO ; Xueji LI ; Hai LIU ; Xun YE ; Xiaolin CHEN
Chinese Journal of Postgraduates of Medicine 2009;32(17):1-4
Objective To examine the diagnosis and outcomes in the treatment of the patients with histologically confirmed central neurocytoma (CNC). Methods The data from 71 patients with CNC who were diagnosed between March 2003 and December 2007 were retrospectively evaluated. Various combinations of surgery, and radiotherapy had been used for treatment. Results The average bulk of tumors was 40 cm3. The median follow-up was 22 months. The 22 months overall survival and local control rate was 95.8%(68/71) and 95.6%(65/68), respectively. Conclusions The overall prognosis is favorable although the follow-up is not very long. Surgery and postoperative radiotherapy can significantly improve local control.
8.Assessment of the Predictive Role of Serum Lipid Profiles in Breast Cancer Patients Receiving Neoadjuvant Chemotherapy
Fanli QU ; Rui CHEN ; Yang PENG ; Ying YE ; Zhenrong TANG ; Yihua WANG ; Beige ZONG ; Haochen YU ; Shengchun LIU
Journal of Breast Cancer 2020;23(3):246-258
Purpose:
Effective predictors of the response to neoadjuvant chemotherapy (NAC) are still insufficient. This study aimed to investigate the predictive value of serum lipid profiles for the response to NAC in breast cancer patients.
Methods:
A total of 533 breast cancer patients who had received NAC were retrospectively studied. The pretreatment of serum lipids, including total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and lipoprotein-α, and clinicopathological characteristics were collected to assess their predictive roles.
Results:
Breast cancer patients had significantly lower TC, TG, HDL-C, and LDL-C levels than normal individuals. Among these indicators, TG and LDL-C levels and HDL-C level increased and decreased significantly after NAC, respectively. In estrogen receptor (ER)-positive patients, increased LDL-C level was associated with better outcomes. Moreover, the receiver operating characteristic curve analyses suggested that TG and HDL-C levels at diagnosis can be used as predictors of the response to NAC only in the ER-positive subgroup.According to univariate analyses, patients with low TG level (< 1.155 mmol/L) or high HDL-C level (≥ 1.305 mmol/L) in the ER-positive subgroup had more favorable clinical responses than the other patients in the subgroup. Furthermore, according to multivariate analyses, a high HDL-C level (≥ 1.305 mmol/L, p = 0.007) was an independent predictor of NAC efficacy.
Conclusion
High HDL-C level (≥ 1.305 mmol/L) before NAC and increased LDL-C level after NAC were associated with the better treatment response in ER-positive breast cancer patients.These results are potentially considered beneficial in establishing treatment decisions.