Cell Adhesion ; drug effects ; Cytokines ; blood ; drug effects ; Humans ; Paraquat ; toxicity

ABSTRACT:Objective To explore the application effect of self-made inflatable mattress in thyroid surgery.Methods A total of 100 patients undergoing thyroid surgeries were randomly di-vided into control group and test groups with 50 cases in each of group.The control group was per-formed surgery with foam position pad on mattress with the area from (50×43×8)cm to (50×43 ×12)cm at 20 degree angles.The test group was performed surgery with controllable self-made inflatable mattress.Blood pressure,heart rate in different positions,comfort degree of surgery pa-tient and satisfaction index was recorded.Results There was no significant difference (P >0.05) between control group and test group in blood pressure and heart rate.Blood pressure and heart rate of patients of surgery in test group were lower than that of control group (P <0.05).There were statistically significant differences since comfort degree of surgery patient and satisfaction in-dex in control group were less than that of test group (P <0.05).Conclusion Controllable self-made inflatable mattress could avoid the changes in vital signs and remain a stable hemodynamics and improve comfort degree of patients and satisfaction index.

ABSTRACT:Objective To explore the application effect of self-made inflatable mattress in thyroid surgery.Methods A total of 100 patients undergoing thyroid surgeries were randomly di-vided into control group and test groups with 50 cases in each of group.The control group was per-formed surgery with foam position pad on mattress with the area from (50×43×8)cm to (50×43 ×12)cm at 20 degree angles.The test group was performed surgery with controllable self-made inflatable mattress.Blood pressure,heart rate in different positions,comfort degree of surgery pa-tient and satisfaction index was recorded.Results There was no significant difference (P >0.05) between control group and test group in blood pressure and heart rate.Blood pressure and heart rate of patients of surgery in test group were lower than that of control group (P <0.05).There were statistically significant differences since comfort degree of surgery patient and satisfaction in-dex in control group were less than that of test group (P <0.05).Conclusion Controllable self-made inflatable mattress could avoid the changes in vital signs and remain a stable hemodynamics and improve comfort degree of patients and satisfaction index.

Objective To construct a recombinant immunotoxin expression vector composed of a single-chain Fv fragment of Sehistosorna japomicum and PE38KDEL gene,and identify the binding activity of the purified product with SEA antigen.Methods The V_H and V_L genes were amplified by PCR from the parent monoclonal antibody NP11-4.Then the amplified scFv and PE38KDEL genes were inserted into the expression vector pBAD/gIII A.The fusion protein expressed in E.coli Top10F' induced by L-arabinose.After purification,the activity of the immunotoxin was evaluated by Westem-blot and ELISA.Results The new recombinant immunotoxin expression vector pBAD/gIII A-scfv-PE38KDEL was constructed successfully.The main product was in inclusion bodies.ELISA assay showed that the refolding recombinant immunotoxin remained binding activity with SEA antigen.Conclusion A new recombinant expression plasmid pBAD/gIII A-scfv-PE38KDEL has been constructed and expressed successfully,which is useful in further study of the treatment of schistosomiasis japonica.

Objective To explore the relationship between the stimulated thyroglobulin ( sTg) and site, number and diameter of metastatic lesions in patients with differentiated thyroid carcinoma (DTC) before the first 131 I treatment, and to evaluate the predictive value of sTg for different metastatic sites. Methods A total of 567 DTC patients (179 males, 388 females; age: (45.3±12.3) years) who received the first 131Ⅰ treatment between January 2012 and June 2017 were included. Thyroglobulin antibody (TgAb), sTg and thyroid stimulating hormone ( TSH) were determined within 1 week before 131 I treatment. Metastases were detected by ultrasonography, CT or 18 F-fluorodeoxyglucose ( FDG ) PET/CT, 131 I whole-body scan, SPECT/CT imaging and pathology. sTg levels of patients with different metastatic sites and different metasta-sis numbers or lesion diameters were compared ( Kruskal-Wallis H test) . Spearman correlation analysis was performed on the number, diameter and sTg level of metastases. The receiver operating characteristic ( ROC) curve was used to explore the predictive value of sTg before the first 131 I treatment for DTC metasta-sis. Results The median values of sTg in the bone, lung, lymph node metastases groups and non-metasta-sis group were 500.00, 104.40, 27.45, 2.39μg/L, respectively, and there were significant differences, ex-cept for bone and lung metastases groups ( H range: -294.605 to 175.162, all P<0.05) . The sTg levels of lung metastasis group and lymph node metastasis group were both decreased by the order of metastasis num- bers (≥3, =2, =1;H range:-57.887 to 48.763, all P<0.05) . As to the diameter of metastases, the sTg levels of >2.0 cm, 1.1-2.0 cm, and≤1.0 cm subgroups in the lung metastasis group and lymph node me-tastasis group were also decreased in order ( H range: -69.935 to 61.043, all P<0.05) . Spearman correla-tion analysis showed that the number ( rs=0.568, 0.606) and diameter ( rs=0.806, 0.664) of the metasta-ses in the lung and lymph node metastases group were positively correlated with sTg (all P<0.05). Areas under ROC curves for sTg to predict bone, lung and lymph node metastasis were 0.935, 0.843 and 0.791 re-spectively. The threshold values were 197. 65, 23. 21 and 10. 96 μg/L respectively. The sensitivities and the specificities were 91.70％, 79.60％, 67.20％ and 97.20％, 80.80％, 82.70％ respectively. Conclusions Tg level before the first 131 I treatment has a certain predictive value for the metastasis, metastatic site and num-ber or diameter in DTC patients.

Objective:To understand changes in psychological resilience of nurses rushing to ICU in Wuhan under COVID-19 epidemics, and provide a theoretical basis for the psychological adjustment and psychological intervention of nurses in public health emergencies.Methods:Using phenomenological research method, the first batch of nurses from Nursing Department of Taizhou People's Hospital to assist Wuhan medical team in February 2020 were selected as the research objects based on Richardson psychological resilience model by the purposive sampling method. The diaries of 9 nurses who assisted Wuhan ICU were used as the original data, and the data collection time was based on the principle of data saturation. The data reached saturation after 18 days of rushing to Wuhan. A total of 9 nurses all completed the diary writing, with a total of approximately 28 452 words. The Colaizzi method was used to analyze the data and extract the topic.Results:A total of 3 first-level themes and 8 second-level themes were extracted, namely the stress period (intrusive thoughts, physical challenges, psychological distress) , buffer zone (psychological capital mobilization, team resilience stimulation, comprehension of social support) and restructuring period (balance restoration, self-transcendence) .Conclusions:Psychological resilience is not a constant psychological feature, but a constantly changing process in the game of destruction and reorganization. The psychological resilience of nurses rushing to ICU in Wuhan is in a dynamic process of stress-buffer zone-reorganization period, while enhancing team resilience and external support is conducive to the healthy development of the balance of psychological resilience of nurses who rushed to wuhan.

Objective:To investigate the effects of methyltransferases like 3(METTL3)mediated m6A modification of double homology cassette A pseudogene8(DUXAP8)on the proliferation,migration and invasion of salivary adenoid cystic carcinoma SACC-LM cells and its potential molecular mechanisms.Methods:Whole-transcriptome sequencing showed that DUXAP8 was highly ex-pressed in SACC than in para-cancerous tissues(P＜0.05).The m6A modification sites on DUXAP8 were predicted using the SRAMP website,and the mRNA and protein expression of m6A-modified genes and the genes associated with the epithelial-mesen-chymal transition(EMT)was measured by qRT-PCR and Western blot,respectively.METTL3 and DUXAP8 was knocked down or overexpressed in SACC-LM cells,and the proliferation,migration,and invasion of the cells were assessed by CCK-8,scratch and Transwell assays.The correlation between METTL3 and DUXAP8 was evaluated using MeRIP-qPCR.Results:The expression of DUXAP8 in SACC tumor was higher than that in para-cancerous tissues(P＜0.05).Knockdown of DUXAP8 reduced proliferation,migration and invasion of SACC-LM cells,as well as the expression of EMT-related genes(P＜0.05).Multiple m6A modification sites of high confidence were found on DUXAP8.METTL3 was highly expressed in tumor tissues,more than other related genes(P＜0.05)and enzyme-encoding genes in SACC-LM cells(P＜0.05).METTL3 was found to function as a methyltransferase to regulate the expression of DUX-AP8,and downregulation of METTL3 inhibited prolifera-tion,migration and invasion of SACC-LM cells and partially reversed the promotion of these activities induced by DUX-AP8 overexpression(P＜0.05).Conclusion:METTL3-me-diated m6A modification upregulated DUXAP8 expression,which promotes the proliferation,migration and invasion of SACC cells.