Objective To study the expression of PINCH and vascular endothelial growth factor C (VEGF-C) in cervical squamous cell carcinoma.Methods We detected the expression of PINCH and VEGF-C by immunohistochemistry SP in 58 cervical squamous cell carcinoma cases and 30 normal cervical epithelial tissue and analyzed their relationship to the clinical pathological features.Results The expression of PINCH and VEGF-C in 58 cervical squamous cell carcinoma(62.1％,36/58 ;67.2％,39/58 ) were higher than that in normal cervical epithelial tissue(0,0/30).The difference was significant( x2 =31.512,12.534,P ＜ 0.001 ).The expression of PINCH protein was not significantly associated with the age,tumor size and tumor differentiation grade ( P ＞ 0.05 ),but was associated with the lymph node metastasis and clinical stage ( x2 =9.090,8.236,P ＜ 0.001 ).The expression of VEGF-C had no significant correlationship with the age and tumor size( P ＞ 0.05 ) but had a correlationship with the lymph node metastasis,tumor differentiation grade and clinical stage( x2 =10.775,13.496,5.001,P ＜ 0.05 ).The expression of VEGF-C was correlated with the expression of PINCH protein( C =0.341,P ＜ 0.01 ).Conclusion It is possible that VEGF-C and PINCH take part in the development and progress of cervical squamous cell carcinoma and play an important role in the invasion and metastasis mechanism altogether.

Objective To analyze the relation of right ventricular outflow tract (RVOT) procedures for repair of tetralogy of Fallot and postoperative right ventricular function in order to improve the postoperative results at mid-long term follow-up.Methods A total of 125 patients for data collection with repair of TOF between January 2003 and January 2006 were prospectively enrolled in the study.Among them,66 were male and 59 were female.The ages ranged from 1 to 5 (3.4 ± 1.1) years,weight from 7 to 26 (15.2 ± 5.7) kg.20 healthy controls with a similar age and body weight were included.The type of RVOT procedure included transannular patch repair (M1) in 67 patients,patch to infundibulum (M2) in 6 patients,tranright-ventricle (RV) no patch repair (M3) in 18 patients and transatrial-transpulmonary no patch repair (M4) in 34 patients.Doppler echocardiogram and tissue Doppler imaging were performed to evaluate RV function.RV systolic function was evaluatedusing tricuspid annular plane systolic excursion (TAPSE) and index of myocardial performance (MPI).RV diastolic function was evaluatedusing ratio of Em/Am and E/Em.Unvariate and logistic regression analysis were used to identify the affective factors.Results In five years fellow-up,MPI and E/Em were higher in patents with M1 and M2 than those in the patients with M3 and M4.Univariate analysis revealed Nakata index,previous shunt operation,operative techniques、postoperative pulmonary regurgitation and during of QRS as risk factors for MPI.In the multivariate analysis,operative techniques and during of QRS were risk factors for MPI.Univariate analysis revealed Nakata index,operative techniques,PR and during of QRS as risk factors for E/Em.In the multivariate analysis,PR was the risk factor for E/Em.Conclusion RV dysfunction is associated with the type of RVOT procedure,diastolic dysfunction is correlative with postoperative PR and systolic dysfunction is correlative with enlarge of RVOT with patch and during of QRS.Echocardiographic imaging is a simple and reliable method for assessment of RV function after repaired TOF.

Objective To investigate the mental health states of health system staffs in a district of Chongqing municipality. Methods Seven hundred and seventy-three health system staffs were taken as the research subjects for conducting the investigation by the Symptoms Checklist-90 (SCL-90).With the total SCL-90 score >160 as the positive psychological symptom,the total mean scores of SCL-90 and the 9 items of factor score of somatization,obsession,personal relations,depression,anxiety,hostility,dread, paranoid and psychotics were performed the statistical analysis.Results The positive rate of psychological symptoms in 773 health system staffs was 17.46% (135/773),among them which in the clinical nurses,clinicians and nonclinical staffs were 23.20%(58/250),15.63%(50/320)and 13.30%(27/203)respectively.The somatization and obsession scores of the health system staffs in this district were obviously higher than those of the national norm (P <0.05),while their personal relations and paranoid scores were obviously lower than those of the national norm(P <0.01).The paranoid scores of the staffs in the district health units were higher than those of the staffs worked in town health units(P <0.05),and other 8 items of factor score and the total mean scores were significantly higher than those in the town health unit staffs (P <0.01).The scores of obsession,depression,anxiety,hostility and the total mean scores of the clinical nurses were significantly higher than those of the clinicians (P <0.01),while scores of so-matization,personal relations,paranoid and psychotics had no significant differences between them(P > 0.05).The total mean scores of the clinical nurses were higher than those of the non-clinical staffs(P <0.05).Conclusion The scores of multiple factors had significant differences between the health system staffs in this district and the national norm.The psychological health status in the clinicians was poorer than that in the non-clinical staffs,especially the psychological health status in the clinical nurses were much poorer.

OBJECTIVETo study the indications, methods and experience of absorbable rib-connecting-pins fixation in the treatment of multiple rib fractures.

METHOD52 cases with multiple rib fractures were performed internal fixation with absorbable rib-connecting-pins under epidural anesthesia.

RESULTSAll cases were followed up for 1 to 12 months, with an average of 5 months. All fractures were achieved healing in 3 to 6 months after the operation and were not found chest wall deformity.

CONCLUSIONSAbsorbable rib-connecting-pins fixation is a simple and effective method and worthies recommending to perform operation for the appropriate cases with multiple rib fractures.

Absorbable Implants ; Adult ; Aged ; Anesthesia, Epidural ; Bone Nails ; Female ; Fracture Fixation, Internal ; instrumentation ; methods ; Humans ; Male ; Middle Aged ; Multiple Trauma ; surgery ; Rib Fractures ; surgery

Objective:To systematically evaluate the actual experience and needs of family caregivers for cardiac arrest patients.Methods:Qualitative research on the real experience and needs of family caregivers in patients with cardiac arrest was electronically searched in databases such as PubMed, CINAHL, and Embase. Two researchers independently screened the literature, evaluated its quality, and integrated the research results. The search period was from database establishment to May 1, 2023.Results:A total of 15 articles were included, and 51 research results were extracted. The similar results were summarized into nine categories and integrated into three results, including sudden changes in life and substantial impacts; challenges in controlling complex emotions, and multiple psychological experiences; multidimensional needs.Conclusions:Family caregivers' actual experiences and requirements for cardiac arrest patients are diverse. Medical and nursing staff need to pay attention to the emotional experiences of family caregivers and meet their multidimensional needs.

The composite material PLGA compounded with β-tricalcium phosphate (β-TCP) was prepared by melt blending method, and the absorbable interface screw was prepared by injection molding process. Prepare PBS buffer that simulates human body, conduct in vitro degradation experiments on interface screws according to relevant national and industry standards, then test and characterize interface screws at different time points for degradation of intrinsic viscosity, average molecular weight distribution, mass loss, mechanical properties and thermal properties. According to the degradation performance-time curve, determine the time node at which the interface screw loses the mechanical properties. In this paper, the in vitro degradation behavior of interfacial screws prepared from PLGA and β-TCP composites was studied in detail, providing a reference and basis for the degradation behavior of absorbable products prepared from PLGA and β-TCP composites.
Humans ; Polyesters ; Materials Testing ; Calcium Phosphates ; Absorbable Implants

Objective:To investigate the early effect of repairing acetabular cartilage delamination with absorbable anchor under hip arthroscopy compared with conventional non-intervention.Methods:Retrospective cohort study was adopted. There were 24 Femoroacetabular Impingment (FAI) patients with acetabular cartilage delamination (ACD) receiving hip arthroscopy surgery in Peking University Third Hospital from May 2021 to August 2021. There were 14 males and 10 females with an average age of 36.3±7.2 years (range 23-53 years). There were 12 patients receiving acetabular cartilage repair with absorbable anchor (absorbable anchor group), and 12 patients in non-intervention group. The X-ray film indicators, α angle, lateral center edge angle (LCEA) and MRI measurement (acetabular cartilage gap, CG) were compared between the two groups. The pre- and post-operative hip functions were assessed by modified Harris Hip Score (mHHS), 12-item international hip outcome tool (iHOT12), hip outcome score-activities of daily living subscale (HOS-ADL), and hip outcome score-sports subscale (HOS-SS), along with visual analogue scale (VAS). The complications (infection, lower extremity deep venous thrombosis) were followed up, as well as the revisional hip arthroscopy surgery and total hip replacement surgery.Results:A total of 24 patients were followed up for 12.0±1.2 months (range 10-14 months). There was no significant difference between the two groups for age, BMI, and symptom onset time ( P>0.05). There was no significant difference between the two groups for the pre-operative α angle, LCEA, CG, mHHS, iHOT12, HOS-ADL, HOS-SS, and VAS scores ( P>0.05). At the last follow-up, there was no significant difference between the two groups for the α angle, LCEA, CG, mHHS, iHOT12, HOS-ADL, HOS-SS, VAS and satisfaction ( P>0.05). In the absorbable anchor group, the α angle was 47.2°±2.6° vs. 63.4°±3.3°, CG was 3.0±0.7 mm vs. 3.3±0.6 mm; mHHS was 73.6±16.0 vs. 57.7±15.4; iHOT12 was 67.6±22.5 vs. 50.6±15.0 after and before the surgery, with significant improvement ( P<0.05). As for the non-intervention group, there was significant decrease of post-operative α angle of 47.4°±2.6° compared to the pre-operative angle of 58.4°±8.1° ( P<0.05). There was no significant difference in LCEA, CG, mHHS, iHOT12, HOS-ADL, HOS-SS, and VAS scores before and after the surgery in non-intervention group comparison ( P>0.05). No complications, revision hip arthroscopy surgery or total hip replacement surgery occurred during the follow up. Conclusion:Good effect was yielded for absorbable anchor repairing acetabular cartilage delamination under hip arthroscopy, without additional risk of complications or revision surgery.