1.MRI Study of Traumatic SCIWORA Analysis of 67 Cases
Jianmin XU ; Zhenjun ZHANG ; Minli WANG
Chinese Journal of Rehabilitation Theory and Practice 1999;5(1):20-23
The authors retrospectively analysed 67(63 in cervical cord)cases of traumatic SCIWOAR with positive MR abnormalities. It was deduced that the cases of SCIWOAR might be related with (1)those traumatic forces resulting in SCI being not so heavy and severe, (2)the cases orignally with cervical spondylopathy,(3)the limitation of the conventional X-ray examination for SCI,and (4)the cases of children relativly rare in our country and hospital.The ALL hematomas are the pathognomonic signs in the case of the acute cervical SCI within one week.The analysis demonstrated that MR investigation of the traumatic spine could give the clinical activities the correct diagnosis of different degree of SCI and dependable reference.
2.The plasma expressions of monocyte chemotaxis proteins in patients with Stanford type A aortic dissection
Fudong FAN ; Zhenjun XU ; Qing ZHOU ; Dongjin WANG
Chinese Journal of Thoracic and Cardiovascular Surgery 2016;32(4):227-229
Objective To explore the plasma expressions of monocyte chemotaxis proteins(MCPs) in patients with type A aortic dissection and their clinical significance.Methods 51 patients with type A aortic dissection were enrolled,in which 29 combined with hypertension.Fourteen hypertension patients and 1 1 hcalthy subjects were enrolled as control.Plasma samples were collected and we examined the levels of MCP-1,MCP-2 and MCP-4 by enzyme linked immunosorbent assay (ELISA).Results Plasma levels of MCP-1 and MCP-2 significantly decreased in patients with type A aortic dissection compared to healthy subjects(P <0.001),while MCP-4 had no change.Type A aortic dissection patients with or without hypertension both showed significant decreased plasma MCP-1 and MCP-2 levels compared to hypertension patients(P <0.001).There was no change of MCP-4 among different groups.Furthermore,Spearman correlation analysis showed that there was no correlation between serum CRP levels and plasma MCP-1,MCP-2 concentrations.Conclusion Plasma MCP-1 and MCP-2 may participate in the pathogenesis of type A aortic dissection,and their concentrations were not correlated with hypertension or serum CRP levels.The detailed mechanism needs further observations.
3.Yinlingtong capsules reduced the accumulation of fat in HHL-5 hepatocytes
Guining LIANG ; Zhenjun XU ; Leihua WENG ; Yayi HOU
International Journal of Traditional Chinese Medicine 2013;(3):225-227
Objective To study the effects of the Yinlingtong capsules on intracellular triglyceride,fatty acid oxidation levels and CPT-1/ACC-β in HHL-5 cells.Methods After treated HHL-5 cells with different doses Yinlingtong capsules,intracellular triglyceride content and the fatty acid oxidation level were detected by the kits.The CPT-1β and ACC-β mRNA levels were detected by Q-PCR.Results Yinlingtong capsules could reduce triglyceride content (compared with the control group,triglyceride content of0.5,1 and 2 mg/ml dose group were reduced by 9.5%,14.3% and 19.4%,respectively),enhance the level of fatty acid oxidation by a dose-response relationship(compared with the control group,the level of fatty acid oxidation of 0.5,1and 2 mg/ml dose group were increased 4.3%,6.9% and 11.2%,respectively),but could not affect the CPT-1βand ACC-β mRNA levels.Conclusion Yinlingtong capsules reduced the accumulation of fat in HHL-5 hepatocytes,and the specific impact mechanism needs to be further explored.
4.Analysis of influencing factors of mechanical ventilation time in esophageal cancer patients with lung infection after operation
Zhenjun LIU ; Jiayu WU ; Pingyao XU ; Cuanze XIONG
Chinese Journal of Primary Medicine and Pharmacy 2011;18(20):2778-2780
ObjectiveTo explore the influencing factors of mechanical ventilation time in esophageal cancer patients with lung infection after operation.Methods55 esophageal cancer patients with lung infection after operation requiring endotracheal intubation or tracheostomy and mechanical ventilation were included.According to duration of mechanical ventilation time, < 7 days for early weaned from ventilator group, > 7 days for late weaning group.60 esophageal cancer patients with lung infection after operation without mechanical ventilation served as controls.Various factors on the impact of mechanical ventilation were compared.ResultsEarly and late weaning patients were older than control group(P < 0.05), particularly in late weaning patients(P < 0.01).Compared with the control, FEV1 and MVV decreased significantly in early weaning patients(P < 0.05).NFGNB infection in late weaning patients significantly increased than in early weaning patients and the control(all P < 0.01).Logistic multivariate analysis showed that FEV1 was independent factors affecting early weaning (P < 0.01), age (P < 0.05) and NFGNB infection (P <0.01) affecting late weaning.ConclusionThe influencing factors of mechanical ventilation time in esophageal cancer patiens with lung infection after operation were FEV1 decreasing,age and NFGNB infection.
5.Experience of Bilingual Teaching to Undergraduate Education at Medical University
Ruxiang XU ; Zhenjun ZHOU ; Huosheng ZHU ; Zhen HUANG ; Heqing LIANG
Chinese Journal of Medical Education Research 2006;0(12):-
Bilingnal teaching has been practiced to undergraduate eduction at medical university.At the initial period,the bilingual teaching to undergraduate.eduction is not so acceptable for lack of the experionce,textbooks and insufficient commnication between teachers and students.This paper discussed the benefits and applience of bilinqual teahing to undergraduate eduction at ncedica university.
6.MRI Study of Tethered Cord
Jianmin XU ; Zhenjun ZHANG ; Shan LIN ; Minli WANG
Chinese Journal of Rehabilitation Theory and Practice 1998;4(2):76-79
The analysis of 87 cases of MR imaging given impression of dysraphic myelodysplasias and their clinical materials found tethered cord deformity in 70 and non - "tethered" cord or "free" cord abnormalities in 17.The purpose of this study was to show that tethered cord was the most important clinical entity resulting in nervous disfunction of the spinal cord of the children among various dysraphic abnormalities and that MRI investigation was the best choice in order to obtain early and correct diagnosis and management of dysraphic myelodysplasias and tethered cord particularly.
7.Cloning, expression and purification of Nocardia brasiliensis proteion P61 with biological activity
Xingzhao JI ; Lu TANG ; Xuexin HOU ; Lina SUN ; Chao WEI ; Shuai XU ; Chenchen SI ; Zhenjun LI
Chinese Journal of Zoonoses 2017;33(3):260-263
We constructed prokaryotic recombinant expression vector of P61 gene from Nocardia brasiliensis,expressing P61 protein with biological activity in E.coli,and lay a foundation for further studies related to P61.P61 gene was synthesized and cloned into an expression vector pET-30a(+).The recombinant vector was transformed into Escherichia coli BL21 and induced with IPTG.The production was analyzed with Western blot and the catalase activity of P61 was tested with Catalase Assay Kit.The protein of P61was successfully expressed in E.coli with solubility and high catalase activity,and could be identified by anti-N.brasiliensis sera from mice.The prokaryotic expression plasmid of protein P61 was constructed successfully and can be expressed efficiently in E.coli BL21 cells with higher catalase.
8.The application of Problem-based learning for scene teaching in "Surgery"
Yuanyuan HUANG ; Zhenjun ZHOU ; Zhen HUANG ; Ruxiang XU ; Zongfang ZHOU ; Heqing LIANG
Chinese Journal of Medical Education Research 2002;0(01):-
Scene teaching is to simulate the clinical scene in class.To cultivate the studnts’abilities of thinking,search and practice in clinical practice,the second clinical college ap-plied the problem-base learning(PBL) in surgery teaching,creating the scene with the standard-ized patients and teachers and drawing out the content from the problems.This article is to summarize the teaching practice.
9.Preparation and Quality Evaluation of Propylthiouracil Solid Lipid Nanoparticles
Xiongbo XU ; Yemei CUI ; Hongjuan SONG ; Zhenjun PU ; Qingsong ZHANG ; Jing TANG ; Ning MA
China Pharmacy 2016;27(7):946-948,949
OBJECTIVE:To optimize the formulation and preparation technology of Propylthiouracil(PTU)solid lipid nanopar-ticles(PTU-SLN)and to evaluate the quality of PLN-SLN. METHODS:PTU-SLN was prepared by emulsion ultrasound dispersing method. The formulation of PTU-SLN was optimized by orthogonal design with the entrapment efficacy and particle size as index, using the amount of lipid material,soybean lecithin and poloxamer 188 and ultrasonic time as factors. The quality of prepared nanoparticles was evaluated with particle size,Zeta potential,entrapment efficiency,stability and in vitro drug release rate as in-dex. RESULTS:The optimal formulation and technology was as follows as lipid material 0.6 g,soybean lecithin 1.0 g,poloxamer 188 0.8 g,ultrasonic time 10 min. The obtained PTU-SLN was round and smooth in appearance and distributed evenly in particle size with average particle size of 93.5 nm,Zeta potential of -30.8 mV and average entrapment efficiency of 74.9%. Prepared nanoparticles had no significant change after placing for 15 d at 4 ℃. Accumulative release rate of PTU-SLN was 56.1% at 4 hour in vitro and reached 98.4% at 24 hour. CONCLUSIONS:PTU-SLN is prepared successfully and reasonable in technology,and can reach sustained-release effects.
10.Reliability and correlation of in vivo heptic shimming of liver and water suppression with 3.0T proton MRS
Li XU ; Changhong LIANG ; Zaiyi LIU ; Zhenjun ZHAO ; Biao HUANG ; Qiongxin ZENG ; Yuanqiu XIAO
Chinese Journal of Medical Imaging Technology 2009;25(7):1208-1210
Objective To assess the reliability of auto-shimming line width (LW) and water suppression rate (WS), and the correlation between them. Methods GE Signa Excite HD 3.0T system and eight-channel torso phased-array coils with PRESS sequence were performed in 38 volunteers. Liver spectra were collected with TR of 1500 ms, TE of 30 ms, VOI of 2 cm×2 cm×2 cm, NSA of 64 times. Spectroscopy routine auto-shimming pre-scanning program was executed and the values of LW and WS were recorded. Then another spectroscopy routine auto-shimming pre-scanning program was performed repeatedly and 38 groups of data were obtained totally. Intra-class correlation coefficients (ICC), coefficient of variation (CV) and significance test were conduced on 38 groups of LW and WS data. Spearman rank correlation analysis was used to assess the correlation of LW and WS. Results ①The ICC of LW and WS was 0.862 and 0.961 (both P<0.0001), respectively, while the value of CV was 0.20, 0.18, 0.09 and 0.08, respectively. Significant difference was not observed; ②The value of correlation coefficient was -0.659, -0.485 (both P<0.0001), respectively. Conclusion ①The reliability is excellent for in vivo liver 3.0T 1H-MRS and WS appears relatively stable; ②Indexes of LW correlate with WS moderately, and it seems the smaller the value of LW is, the easier to achieve higher WS.