Eosinophilic esophagitis(EoE)is a chronic autoimmune inflammatory disease characterized by accumulation of eosinophils in all layers of esophagus and lacking typical clinical manifestations. The pathogenesis has not yet been clarified. Diagnosis is mainly by combined judgment,including clinical manifestation,endoscopy and histopathology. Treatment includes food,drug and surgical therapy. This article reviewed the advances in study on EoE.

OBJECTIVE:To study the situation of anemia in newly diagnosed ovarian cancer and breast cancer patients treated with 7 kinds of chemotherapy regimes. METHODS:110 cases of newly diagnosed ovarian cancer and breast cancer from May 2008 to Feb. 2009 in People’s Hospital of Peking University were analyzed retrospectively. The level of hemoglobin in patients treated with different chemotherapy regimes were evaluated and analyzed with SPSS software. RESULTS:Of total 110 patients, class Ⅰ anemia occurred 194 times and class Ⅱ anemia occurred 55 times. High incidence of anemia appeared in chemotherapy regimes with platinum as base. Low incidence of anemia occurred in TA chemotherapy regime. The older patient is,the more easily anemia occurred. CONCLUSION:The incidence of anemia after chemotherapy is related to type of cancer,chemotherapy regime, chemotherapy cycle and patient’s age.

ObjectiveTo analyze the endemic situation of schistosomiasis in national surveillance sites of Zhenjiang City from 2015 to 2019, so as to provide the scientific basis for the consolidation of schistosomiasis control achievements and timely treatment of risk factors. MethodsA total of 7 national schistosomiasis surveillance sites were assigned in Zhenjiang City from 2015 to 2019. According to the National Scheme for Schistosomiasis Surveillance (2014 version), Schistosoma japonicum infections were monitored in local residents, floating populations and livestock, and snail habitats were surveyed in Zhenjiang City from 2015 to 2019. The results of schistosomaisis surveillance in Zhenjiang City from 2015 to 2019 were analyzed. Results There were 15 848 and 7 779 person-times of local residents and floating populations receiving serological screening of schistosomiasis in 7 national surveillance sites of Zhenjiang City from 2015 to 2019, and the seroprevalence rates were 0.95% to 1.75% and 0.14% to 0.91% among local residents and floating populations, respectively; however, no egg-positives were identified. A total of 1 885 herd-times of livestock were detected, and no egg-positives were found. During the 5-year period, snail survey was conducted in snail habitats and suspected snail habitats covering 3 333.79 hm², and there were 108.71 hm² snail habitats identified, including 50.97 hm² re-emerging snail habitats. The density of living snails was 0.007 to 0.125 snails/0.1 m² from 2015 to 2019, which showed a trend for a rise year by year (χ² = 2 732.626, P < 0.01); however, no S. japonicum infections were detected in snails. Conclusions The endemic situation of schistosomiasis has been maintained at a low level in Zhenjiang City, and schistosomiasis control achievements are consolidated. However, snails are widely distributed in the city, and there are still risk factors associated with schistosomiasis transmission. Further integrated interventions require to be reinforced to timely manage the risk of transmission of schistosomiasis and promote the progress towards elimination of schistosomiasis.

Objective To investigate the prevalence of parasitic infections in freshwater fish and marine fish from markets in Zhenjiang City, so as to provide the evidene for formulating targeted control measures. Methods Freshwater fish and marine fish were collected from farmers’ markets and supermarkets in Zhenjiang City using a random sampling method. The metacercaria and larvae of parasites were detected using microscopy and the direct dissection method. The prevalence of parasitic infections was analyzed in freshwater fish and marine fish. Results The overall prevalence of parasite infections was 11.21% (208/1 856) in the 1 856 fish (34 species) detected from 2017 to 2020, and the prevalence was 9.81% (117/1 193) in freshwater fish and 13.73% (91/663) in marine fish. Among the 1 193 freshwater fish (23 species), high prevalence of parasitic infections was detected in Gambusia affinis (43.33%), coarse fish (32.93%) and Hemiculter leucisclus (20.20%), and there was no significant difference in the prevalence of parasitic infections among different species of freshwater fish (χ² = 105.66, P < 0.05). Clonorchis sinensis, Metorchis orientalis and other parasite species were detected in freshwater fish, with detection rates of 0.59% (7/1 193), 1.93% (23/1 193) and 7.29% (87/1 193), respectively, and there was a significant difference in the detection of C. sinensis and M. orientalis in freshwater fish (χ² = 8.64, P < 0.05). The prevalence and intensity of larval Anisakis infections were 13.73% (91/663) in marine fish and 3.43 parasites per fish. High prevalence of Anisakis infections was seen in little yellow croaker fish (30.28%) and hairtail (27.50%), and there was a significant difference in the prevalence of parasitic infections in different species of marine fish (χ² = 12.93, P < 0.05). Conclusions Parasitic infections are prevalent in freshwater fish and marine fish from markets of Zhenjiang City, and there is a risk of parasitic infections following consumption of raw or under-cooked freshwater fish and marine fish.

Objective To explore the expressions of interleukin (IL)-17A and its receptor IL-17RA in different degrees of malignant gliomas. Methods Fifty patients with glioma were collected in this study. Accordance to the World Health Organization Classification System, patients were classified by malignancy grade, including gradeⅠ(n=12), gradeⅡ(n=18), gradeⅢ(n=13) and gradeⅣ(n=7). The glioma tissue and peripheral blood samples of patients were obtained for detecting the expression levels of IL-17A and IL-17RA mRNA by using immunohistochemistry, quantitative real-time PCR. Western blot assay was used to detect expressions of IL- 17A and IL- 17RA in both the macroscopic (immunohistochemistry) and molecular levels (mRNA and protein). Results Immunohistochemical staining showed that the expression levels of IL-17A and its receptor IL-17RA increased with the increase of the malignant degree of gliomas. The mRNA levels of IL-17A and IL-17RA receptors in peripheral blood were up-regulated with the increasing malignancy grade of glioma (F=8.96, P<0.05;F=10.34, P<0.05). The mRNA levels of IL-17A and IL-17RA in glioma tissues were up-regulated with the increasing malignancy grade of glioma (F=11.21, P<0.05;F=14.11, P<0.05). The protein levels of IL-17A and IL-17RA in peripheral blood and glioma tissues were also up-regulated with the increasing malignancy grade of glioma (in peripheral blood:F=9.90, P<0.05;F=11.80, P<0.05;and in gliomas tissues:F=8.15, P<0.05;F=14.46, P<0.05). Conclusion The expressions of IL-17A and IL-17RA receptor are positively correlated with malignancy grade of glioma. These results provide some reference for clinical diagnosis of malignant gliomas.

Objective To investigate the effect of percutaneous eoronary intervention (PCI) on the prognosis of acute ST-segment elevation myocardial infarction (ASTEMI) in the elderly.Methods The 1318 ASTEMI patients in our hospital from June 1998 to June 2008 were retrospectively analyzed. Among them, 338 (25.6%) elderly patients were over 60 years old, and 316patients consistent with inclusion and exclusion criteria were consecutively enrolled in our research.Then they were divided into two groups: PCI group (136 cases, 43.0%) and conservative drug treatment group (180 cases, 57. 0%). The clinical data of study objects were collected. Then they were followed up regularly for two years. Results There were no statistically significant differences between the two groups in mean age, gender, hypertension, diabetes, dyslipidemia, excess smoking,wine and family history (all P＞ 0.05). And there were no statistically significant differences in anterior wall STEMI, Killip Ⅲ-Ⅳ class, thrombolysis therapy and malignant ventricular arrhythmia (all P＞0. 05). Most of the objects proceeded therapeutic lifestyle improvements, such as giving up smoking, restricting wine, regulating diet, losing weight and insisting on exercises, and so on.Secondary prevention drugs of acute myocardial infarction including angiotensin converting enzyme inhibitor, angiotensin receptors blockers, beta receptor, aspirin and statins were regularly administrated in the two follow-up years. In the retrospective research, incidence rates of reinfarction, NYHA (New York Heart Association) Ⅲ-Ⅳ class heart function and one-month mortality were much higher in conservative treatment group than in PCI group (17.2% vs. 2. 2%, OR=9. 224,95% CI: 2. 756-30. 857; 31.1% vs. 8.1%,OR=5.132, 95%CI: 2. 568-10. 257; 8. 3% vs. 1.5%,OR= 6. 091, 95% CI: 1. 369-27. 105, respectively; all P ＜ 0. 01). Above all, one and two-year mortalities were much higher in conservative treatment group than in PCI group (21.1% vs. 2. 2 %,OR=11.864, 95%CI: 3.577-39.349; 32.2% vs. 4.4%, OR=10.301, 95%CI: 4.289-24.736,respectively; all P＜0. 01). Conclusions PCI may reduce the re-infarction, NYHA Ⅲ-Ⅳ class heart function and one-month mortality, especially so in view of the one and two-year mortality. PCIcan significantly improve the prognosis of ASTEMI in the elderly.

Objective:To investigate the correlation between positive rate of thyroid autoantibodies in healthy people and thy -roid dysfunction.Methods:Fasting blood from 3218 healthy examined people in Cangzhou were collected and the serum was separa -ted.Automatic electrochemiluminescence immunoassay systems and reagents were used to determine the concentration of thyroid stimu -lating hormone(TSH),free triiodothyroninthyroid(FT3),free thyroxine(FT4),peroxidase antibody(TPOAb)and thyroglobulin antibody ( TgAb ) .TPOAb>34 U/ml and TgAb>115 U/ml was positives.Statistical analysis of test results .statistica analysis of test reults.Results:The positive rate of thyroid autoantibodies of tested population was 16.19%.The positive rates of TPOAb and TgAb were 14.57%,12.74%separately.The detection rate of TPOAb and TgAb was 11.12%in tested population.The positive rate of TPO-Ab and TgAb and TPOAb+TgAb were 6.92%,5.68%,5.03%separately in 1532 male subjects and were 21.53%,19.16 ,16.67%separately in 1686 female subjects,which were significantly higher in female group than that in male group (P<0.001).They gradually increased with age and reached a peak above 70 years old in female group and in 50-59 in male group.The positive rate and OR of thy-roid autoantibodies in hyperthyroidism and subclinical hyperthyroidism and in hypothyroidism and subclinical hypothyroidism and in hy -pothyroidism and subclinical hypothyroidism were statistically significant .Conclusion:The positive incidence of thyroid autoantibodies is higher in healthy people of Cangzhou .It is essential to follow-up these people of normal thyroid function with positive autoantibodies in order to facilitate prevention and early diagnosis and treatment of thyroid disease .

Objective: The present study investigated the clinical effects of a single complete denture using two-step fine impression during the restoration of mandibular alveolar ridge atrophy. Methods : A back-end window tray that was personalized and adjusted was used to obtain the original impression for a mandibular alveolar ridge case. The individual trays were made with the conception of mandibular occlusal denture, and the final impression was obtained after active edge shaping using different viscous silicones. Results : A case of single complete denture with mandibular alveolar ridge atrophy restoration was accomplished using a single complete denture and two-step fine impression. The denture was well fixed and functioned well, and the patient was satisfied. Conclusion The application of fine impression into a single complete denture is helpful for the restoration of mandibular alveolar ridge atrophy.

ObjectiveTo investigate the relationship of the expression of vasodilator-stimulated phosphoprotein (VASP) with the metastasis and prognosis of prostate cancer.

METHODSProstate cancer PC3 cells were infected with VASP shRNA and control shRNA lentiviruses, respectively. The invasive ability of the PC3 cells was determined by transwell migration assay, the expression of VASP in the prostate cancer tissue from 56 patients was detected by immunohistochemistry, and the survival rate of the patients was analyzed according to the VASP expression levels and follow-up data after radical prostatectomy.

RESULTSVASP shRNA lentivirus significantly inhibited the expression of VASP and decreased the invasive ability of the PC3 cells as compared with the results obtained in the scramble shRNA and blank control groups (P<0.05). The survival analysis of the 56 prostate cancer patients showed that the time of biochemical recurrence was markedly shorter in the VASP positive and strongly positive groups than in the VASP-negative cases (P<0.05), but with no statistically significant difference between the former two groups (P>0.05).

CONCLUSIONSVASP is involved in the regulation of the invasive ability of prostate cancer PC3 cells, and the differences in the VASP expression are related to the prognosis of prostate cancer.

Cell Adhesion Molecules ; metabolism ; Cell Line, Tumor ; Humans ; Immunohistochemistry ; Lentivirus ; Male ; Microfilament Proteins ; metabolism ; Neoplasm Metastasis ; physiopathology ; Phosphoproteins ; metabolism ; Prognosis ; Prostatectomy ; Prostatic Neoplasms ; pathology ; surgery ; RNA, Small Interfering ; Survival Rate

Objective : To detect the expression of protein arginine methyltransferase 5 (PRMT5) in gastric cancer tissues and gastric cancer cells , and to investigate the regulation of PRMT5 on the proliferation , migration , invasion and epithelial mesenchymal transition (EMT) of gastric cancer cells. Methods : ① The expression of PRMT5 in pathological tissues of chronic non⁃atrophic gastritis , and pregastric cancer, including chronic atrophic gastritis and intestinal metaplasia , and early gastric cancer was analyzed based on Bioinformatics Analysis by GEO database. Ualcan and HPA databases were employed to analyze the expression of PRMT5 in gastric cancer tissues. The expression of PRMT5 in gastric cancer cells was detected by Western blot. ② The expression of PRMT5 in gastric cancer cells was regulated by plasmid , and its efficiency was verified by Western blot and RT⁃PCR. The protein expression of PRMT5 in gastric cancer cells was analyzed via Western blot. The abilities of migration and invasion were examined by scratch assay , Transwell and BD Matrigel invasion assays. Clone formation assay and CCK⁃8 assay were used to examine the proliferation of gastric cancer cells. ③ The expression of interstitial⁃related proteins and epithelial⁃related proteins was evaluated via Western blot. Results : GEO database found that PRMT5 expression increased gradually in chronic non⁃atrophic gastritis , precancerous lesions and early gastric cancer. Ualcan andHPA databases found that PRMT5 in gastric cancer tissues were higher than that in normal gastric tissues both at the HPA databases found that PRMT5 in gastric cancer tissues were higher than that in normal gastric tissues both at the mRNA and protein levels. PRMT5 upregulation elevated the migration , invasion and proliferation of gastric cancer cells , while PRMT5 downregulation inhibited those. In addition , PRMT5 upregulation raised the expression of interstitial⁃related proteins and decreased the expression of epithelial⁃related proteins while PRMT5 downregulation was the opposite. Conclusion PRMT5 is relatively highly expressed in gastric cancer tissues. Furthermore , PRMT5 can enhance the migration , invasion and proliferation ability of gastric cancer cells , and promote EMT in gastric cancer.