Objective To compare the effects of sufentanil and fentanyl on hemodynamics and stress to induction of general anesthesia in children.Methods According to the digital table,78 children undergoing selective operations were randomly divided into sufentanil group(S group) and fentanyl group(F group),39 cases in each group.Anesthesia was induced with sufentanil 0.3μg/kg or with fentanyl 3μg/kg.Mean arterial pressure(MAP), heart rate(HR) and the values of norepinephrine (NE),epinephrine(E),cortisol(Cor) were detected at five time points:2 minutes before anesthesia(T0),the time of tracheal intubation(T1),1 min after intubation(T2),3 min after intubation(T3) and 5 min after intubation(T3).Results The values of MAP,HR were higher at any time point after intubation in both groups(all P<0.05),and they were significantly higher in F group than those in S group(all P<0.05).The concentrations of NE,E,Cor have a significant increasing after endotracheal intubation in F group than those in S group(all P<0.05).There were no significant differences in NE,E,Cor concentrations at different time points in S group(all P>0.05).Conclusion Sufentanil has more inhibitory effect on stress response and provides more stable hemodynamic than fentanyl to induction of general anesthesia in children.

Objective To observe the effect on Foxg1 gene expression in the subgranular zone (SGZ) of cerebral tissue from neonatal rats with hypoxic-ischemic brain damage (HIBD) after transplantation of neural stem cells (NSCs) derived from umbilical cord blood.Methods Mononuclear cells separated from umbilical cord blood by density gradient centrifugation were cultured with orientated induction to differentiate the NSCs.The neuronal phenotype was identified using immunocytochemical methods.A total of 150 Sprague-Dawley rats were randomly divided into a sham-operation group,an HIBD group and an HIBD-NSCs group.Rats in the HIBD group and the HIBD-NSCs group were subject to ligation of the left carotid artery and then kept in a box under 8％ oxygen and 92％ nitrogen for 2.5 hours to establish the HIBD animal model.The artery was separated but not ligated in the sham operation group,which was not subjected to hypoxia.Twenty-four hours after the operation,the cultivated NSCs were transplanted by caudal vein injection into the rats in the HIBD-NSCs group.Rats were then sacrificed on the 3rd,7th,14th,21st and 28th days after the operation.Foxg1 gene expression in the SGZ was examined using in-situ hybridization methods.Results The number of Nestin-positive cells peaked on the 6th day of cultivation and then decreased by the 9th day.The Foxg1 gene was expressed in the SGZs of each group.The expression increased by the 3rd day after surgery in the HIBD and HIBD-NSCs groups,and peaked on 7th day after the operation,then declined gradually.The average expression level of Foxg1 in the HIBD group was significantly lower than that in the HIBD-NSCs group on the 7th day and thereafter.Conclusions Human umbilical cord blood mesenchymal stem cells can be induced and differentiated into neural stem cells.Foxg1 genes can still be present in the SGZ after birth.HIBD can induce the expression of Foxg1 genes.Transplanting NSCs can promote the expression of Foxg1 genes and improve morphological and functional recovery after HIBD,at least in neonatal rats.

Objective To detect gap junction protein Cx45, gap junction protein Cx40 and Tumor Necrosis Fαctor-α(TNF-α) expression in the infarcted myocardium, and to study their combined effects. Methods Immunohisto-chemical staining (S-P immunohistochemical staining method) and qRT PCR detection of gap junction were used to identify protein Cx45, Cx40 and TNF-α respectively in 30 cases of late early myocardial ischemic stroke group and 30 cases of myocardial infarction due to sudden death group with control group being 20 cases of normal expression in myocardial group. The effect of the different expressions was compared in the different groups. Results Immuno-histochemical results: Between the two groups, Cx45 expression was higher in the sudden death group compared to normal myocardium. Cx40 expression was decreased significantly in the sudden death group when compared to normal myocardial group while TNF-α expression was high. The expressions had significant differences in the three groups (P<0.05). Qrt - PCR results showed that the mRNΑ expression of Cx45, Cx40 and TNF-α was consistent with their respective protein expression and that there was statistically significant difference in three groups (P<0.01). Cx45, Cx40 and TNF-α had a correlation in the three groups of cardiac muscle. Conclusion Gap junction protein Cx45, Cx40 and TNF-α expression can be useful indicators for the diagnosis of the cause of sudden cardiac death.

OBJECTIVETo investigate the expression of SFRP4 and DKK1 in cervical squamous cell carcinoma and explore the clinicopathological implications.

METHODSImmunohistochemistry and reverse transcriptase-polymerase chain reaction (RT-PCR) were used to detect the expressions of SFRP4 and DKK1 in 66 cervical squamous cell carcinoma and 26 normal cervical specimens.

RESULTSSFRP4 expression was significantly higher (P<0.01) and DKK1 expression was significantly lower (P<0.05) in the carcinoma tissues than in normal cervical tissues. DKK1 was negatively correlated with SFRP4 in the carcinoma tissues (P<0.01), and their expressions were associated with the clinical stages, tumor differentiation, depth of invasion and lymph node metastasis of the tumors (P<0.05).

CONCLUSIONSFRP4 and DKK1, the upstream components of the Wnt pathway, play a key role in the tumorigenesis of cervical squamous cell carcinoma, and their expressions are associated with the clinicopathological features of the malignancy.

Carcinoma, Squamous Cell ; metabolism ; Female ; Humans ; Immunohistochemistry ; Intercellular Signaling Peptides and Proteins ; metabolism ; Lymphatic Metastasis ; Proto-Oncogene Proteins ; metabolism ; Uterine Cervical Neoplasms ; metabolism

Objective To investigate the expression of SFRP4 and DKK1 in cervical squamous cell carcinoma and explore the clinicopathological implications. Methods Immunohistochemistry and reverse transcriptase-polymerase chain reaction (RT-PCR) were used to detect the expressions of SFRP4 and DKK1 in 66 cervical squamous cell carcinoma and 26 normal cervical specimens. Results SFRP4 expression was significantly higher (P<0.01) and DKK1 expression was significantly lower (P<0.05) in the carcinoma tissues than in normal cervical tissues. DKK1 was negatively correlated with SFRP4 in the carcinoma tissues (P<0.01), and their expressions were associated with the clinical stages, tumor differentiation, depth of invasion and lymph node metastasis of the tumors (P<0.05). Conclusion SFRP4 and DKK1, the upstream components of the Wnt pathway, play a key role in the tumorigenesis of cervical squamous cell carcinoma, and their expressions are associated with the clinicopathological features of the malignancy.

Objective To investigate the expression of SFRP4 and DKK1 in cervical squamous cell carcinoma and explore the clinicopathological implications. Methods Immunohistochemistry and reverse transcriptase-polymerase chain reaction (RT-PCR) were used to detect the expressions of SFRP4 and DKK1 in 66 cervical squamous cell carcinoma and 26 normal cervical specimens. Results SFRP4 expression was significantly higher (P<0.01) and DKK1 expression was significantly lower (P<0.05) in the carcinoma tissues than in normal cervical tissues. DKK1 was negatively correlated with SFRP4 in the carcinoma tissues (P<0.01), and their expressions were associated with the clinical stages, tumor differentiation, depth of invasion and lymph node metastasis of the tumors (P<0.05). Conclusion SFRP4 and DKK1, the upstream components of the Wnt pathway, play a key role in the tumorigenesis of cervical squamous cell carcinoma, and their expressions are associated with the clinicopathological features of the malignancy.

Objective:To construct a risk assessment model based on hesitant probabilistic fuzzy set(HPFS)and to explore its application value in the risk control and management of safe operation of anesthesia equipment.Methods:Taking the whole life cycle safety and management safety as the important risk assessment dimensions,the risk index system of safe operation of anesthesia equipment was constructed,HPFS and hierarchical-superior-inferior solution distance method were used to realize the quantitative analysis of risks,and the safety self-inspection and risk control treatment strategies were formulated.A total of 150 surgical patients and 16 anesthesia equipment used in surgery used in the operation were selected from July 2020 to June 2023 in Tongzhou District Maternal and Child Health Hospital,and into control group and observation group according to different management modes of anesthetic equipment,with 75 cases in each group.The 10 anesthesia machines used during the surgical treatment of the control group adopted the conventional risk control mode,and the 12 anesthesia machines used in the observation group(including 6 in the control group and the 6 newly added ones)adopted the risk assessment control mode.The incidence of perioperative anesthesia equipment-related risk events,the awareness rate of anesthesia medical staff about potential safety risks,and the failure rate of anesthesia equipment were compared between the two groups.Results:The number of perioperative risk events of improper operation of anesthesia equipment,unreasonable dosage of anesthesia,associated infection and missing records in the observation group were 4 cases(5.3%),0 cases(0%),1 case(1.3%)and 1 case(1.3%),respectively,which was lower than that in the control group,the difference was statistically significant(x2=4.478,4.110,6.857,4.754;P<0.05).The average scores of theoretical knowledge of safety management,safe use,management awareness and fault judgment ability of medical staff operating anesthesia equipment in the observation group were(96.27±3.93)points,(94.31±2.69)points,(91.82±1.94)points and(84.97±4.36)points,respectively,which were higher than those in the control group,the difference was statistically significant(t=5.176,5.322,5.541,5.942;P<0.05).The total number of equipment operation setting,anesthetic gas path,anesthesia depth monitoring,threshold alarm and other faults in the two groups were 90,37,25,316 and 125,respectively,and the failure incidence rates in the observation group were 30%(27/90),35%(13/37),28%(7/25),22%(69/316)and 39%(49/125),respectively,which were lower than those in the control group,the difference was statistically significant(x2=28.800,6.541,9.680,200.532,11.664;P<0.05). Conclusion:The risk assessment model based on HPFS can reduce the incidence of risk events related to anesthesia equipment,enhance the awareness of safety risk control of anesthesia medical staff,and improve the quality of clinical operation of anesthesia equipment.

Objective:To evaluate the effect of carbon dioxide pneumoperitoneum and/or body position on stroke volume variation in the patients undergoing gynecological laparoscopy.Methods:Twenty patients undergoing gynecological laparoscopy were included in the study. After routine induction of general anesthesia, all the patients were intubated and mechanically ventilated with a tidal volume of 8 ml/kg and a respiratory rate of 8-12 times/min. General anesthesia was maintained. Stroke volume (SV) and stroke volume variation (SVV) were monitored with the FloTrac/Vigileo system at supine position without pneumoperitoneum (T 1), 15° head-down tilt position without pneumoperitoneum (T 2), supine position with pneumoperitoneum pressure (IAP) of 12 mmHg (T 3), 15° head-down tilt position with IAP 12 mmHg (T 4), supine position with IAP of 15 mmHg (T 5) and 15° head-down tilt position with IAP of 15 mmHg (T 6). Results:There were no significant differences in SV and SVV between T 2 and T 1 ( P>0.05). SVV was significantly increased and SV decreased at T 3-6 than at T 1 and T 2 ( P<0.05). There was no significant difference in SV between T 5 and T 3 and between T 6 and T 4 ( P>0.05). SVV was significantly higher at T 5 than at T 3 and at T 6 than at T 4 ( P<0.05). Conclusions:Although body position has no significant impact on SVV, the carbon dioxide pneumoperitoneum has a greater influence, indicating that SVV is not suitable for assessing blood volume status during laparoscopic surgery.

Objective To investigate the inhibitory effect of salinomycin on human breast cancer cells in vitro, and to explore the related molecular mechanism.Methods Human breast cancer MDA-MB-231 cells were treated with salinomycin at different concentrations and at various time points.The effect of salinomycin on MDA-MB-231 cells proliferation was studied by CCK-8 method.The cell cycle status was examined by flow cytometry.RT-PCR and Western blot were used to detect the expression of Shh, Smo and Gli1 in the Hedgehog pathway at mRNA and protein levels.Results Proliferation of MDA-MB-231 cells treated with salinomycin was markedly inhibited in a concentration and time dependent manner.Salinomycin at concentrations of 0,0.4,0.8 and 1.6 μmol/L inhibited the growth at the rates of 11.18%,25.88%, 50.03%, 92.65%, respectively.Salinomycin prevented MDA-MB-231 cells from G1 into S phase.Salinomycin at concentrations of 0,0.8 and 1.6μmol/L resulted in S-phase percentage of 25.03%,11.85%and 35.21%, respectively ( P <0.05 ).RT-PCR and Western blot showed that the expression of key elements Shh, Smo and Gli1 in the Hedgehog pathway was inhibited by salinomycin in a concentration dependent manner( P<0.05) .Conclusion Salinomycin prevents breast cancer cell transition from G1 to S phase through downregulation of the target genes of Hedgehog signaling pathway, leading to an effective inhibition of MDA-MB-231 cells.

OBJECTIVETo investigate the inhibitory effect of salinomycin on human breast cancer cells in vitro, and to explore the related molecular mechanism.

METHODSHuman breast cancer MDA-MB-231 cells were treated with salinomycin at different concentrations and at various time points. The effect of salinomycin on MDA-MB-231 cells proliferation was studied by CCK-8 method. The cell cycle status was examined by flow cytometry. RT-PCR and Western blot were used to detect the expression of Shh, Smo and Gli1 in the Hedgehog pathway at mRNA and protein levels.

RESULTSProliferation of MDA-MB-231 cells treated with salinomycin was markedly inhibited in a concentration and time dependent manner. Salinomycin at concentrations of 0, 0.4, 0.8 and 1.6 µmol/L inhibited the growth at the rates of 11.18%, 25.88%, 50.03%, 92.65%, respectively. Salinomycin prevented MDA-MB-231 cells from G1 into S phase. Salinomycin at concentrations of 0, 0.8 and 1.6 µmol/L resulted in S-phase percentage of 25.03%, 11.85% and 35.21%, respectively (P < 0.05). RT-PCR and Western blot showed that the expression of key elements Shh, Smo and Gli1 in the Hedgehog pathway was inhibited by salinomycin in a concentration dependent manner (P < 0.05).

CONCLUSIONSalinomycin prevents breast cancer cell transition from G1 to S phase through downregulation of the target genes of Hedgehog signaling pathway, leading to an effective inhibition of MDA-MB-231 cells.