The etiology of uveitis is very complicated and the pathogenesis is not yet clear.In recent years,more and more evidences imply that the chemokines interleukin-8 (IL-8) is involved in the development of uveitis.Studies of IL-8 and its network features contribute to the understanding of uveitis pathogenesis and provide an important foundation for seeking new indication of activity of uveitis and new treatment target.This paper summarizes the progression in the study on IL-8 in uveitis,including the physiology functions of IL-8,mechanisms for ocular inflammatory,and the role and performance of IL-8 in uveitis.

In this article,the author systematically summarized professor SUN Gui-zhi' academic thoughts about pathogeny and treatment of tumour.Professor SUN Gui-zhi thinks that the Phlegm is one of the most important pathogenic evils that induce tumour.As to cold and heat syndrome of tumour,she thinks that heat syndrome is more important.While to defi ciency and excess syndrome,she thinks excess syndrome primary in the early time,deficiency-excess in complexity in intermediate stage,and defi ciency syndrome primary in later period of tumour.In treatment of tumour,professor SUN Gui-zhi pays more attention to dispelling pathogenic evils of phlegm and heat,at the same time protecting stomach qi,and adjusting the helm opening and closing of qi movement.She is good at making complex and large prescription.

Objective The separation of various proteins in urine was used to differentiate the types of urinary protein to determine the site of diabetic kidney damage and lesions. Methods We used sodium dodeeyl sulfonate - agarosegel electrophoresis(SDS - AGE) to detect the unconccntrated urine on 108 cases patients with diabetic nephropathy urine specimens analysis. Results The results of urinary protein electrophoretic of 108 patients displayed: 4 eases for renal tubular proteinuria, 10 eases for physiological proteinuria, 22 eases for glomerular proteinuria, 72 cases for mixed proteinuria. Conclusion Urine protein electrophoresis for diabetic nephropathy patients with positive can distinguish the various components, and to understand the degree of kidney damage and lesions. This method is low -cost, non - invasive and highly sensitive. The film can be stored. It is worthy to be popularized.

Matrix metalloproteinases(MMP) are a family of Zn~(2+)-dependent endopeptidases capable of cleaving components of extracellular matrix(ECM).MMP-1 was the first vertebrate collagenase purified as a protein and cloned as a cDNA,and is considered the prototype for all the interstitial collagenases.It is synthesized as a zymogen.Its N-terminal residues are removed by proteolysis and it shares with other MMPs a catalytic domain and a carboxy terminal domain with the sequence similar to hemopexin.(MMP-1) should be considered as a multifunctional molecule since it participates not only in the turnover of collagen fibrils in the extracellular space but also in the cleavage of a number of nonmatrix substrates and cell surface molecules,suggesting an important role in the regulation of cellular behaviour.Furthermore,an extensive body of evidence indicates that MMP-1 plays an important role in diverse physiologic processes such as growth,tissue morphogenesis,and wound repair.Likewise,it seems to be implicated in a variety of human diseases including atherosclerosis,rheumatoid arthritis,pulmonary emphysema,and fibrotic disorders,suggesting that its inhibition or stimulation may open therapeutic avenues.

Pulmonary arterial hypertension ( PAH) is a progressive lethal vascular disease, which is characterized by pulmo-nary vasoconstriction and vascular remodeling. Recent studies have indicated that the metabolic theory, centered on the loss of mito-chondrial function, plays a key role in the occurrence and development of PAH. This theory links mitochondrial-related changes of ap-optosis-resistance, glycolysis enhancement, mROS decrease and aberrant mitochondrial dynamics to multiple molecular abnormalities, which may provide new ideas for exploring more comprehensive and specific treatment strategies. This article reviews the progresses in mitochondrial abnormality in PAH.

Objective To investigate the effects of inhalation of different concentrations of nitrous oxide on intracuff pressure (ICP) of the laryngeal mask airway (LMA) in patients during general anesthesia. Methods Forty-right ASA Ⅰ or Ⅱ patients aged 25-64 yr undergoing elective operations under general anesthesia were randomly divided into 4 groups (n=12 each): group C (100 %O2, group N2 (65 % O2 + 35%N2O), group N2 (50%O2 + 50 % N2O), and group N3 (35%O2 + 65%N2O). A suitable model of the LMA was selected according to height and weight. Anesthesia was induced with Ⅳ propofol 1.5-2.0 mg/kg, fentanyl 2-3 μg/kg, lidocaine 1-2 mg/kg and vecuronium 0.08-0.1 mg/kg and maintained with intravenous infusion of propofol and intermittent boluses of fentanyl and vecuronium. After the successful placement of LMA, the air was injected into the cuff to make intracuff pressure (ICP) reach 40 cm H2O (baseline). The ICP were recorded at 15, 30, 45, 60, 75 and 90 min (T1-6) following inhalation of N2O. Results The ICP grandually increased with the increase in the concentration of N2O (P<0.05). The ICP decreased at T2-6 in group C, but increased at T2-6 in group N1 and at T1-6 in group N2,3 as compared with baseline(P<0.05). There was a positive correlation between the ICP and inhalation time in group N1-3(r1-3=0.968,0.987,0.973 respectively, P<0.05). Conclusion The inhalation of N2O can increase the ICP in concentration- and time-dependent manners,

Objective To investigate the clinical effect of psychological intervention combined with milrinone on the treatment of the children with congenital heart disease complicated with severe pneumonia and heart failure. Methods In the First People's Hospital in Jining City from February 2015 to December 2016, 100 children with congenital heart disease complicated with severe pneumonia and heart failure as the research object, randomly divided into the control group and the experimental group, 50 cases in each group. The control group were received routine treatment, including ECG monitoring, anti infection and oxygen therapy,and the treatment of rice farmers. The experimental group was treated with milrinone treatment, psychological intervention, pay attention to the mental health of patients. The therapeutic effects of the experimental group and the control group were compared and analyzed. Results after the corresponding treatment, the patients in the experimental group and the control group were mild and no serious adverse reactions occurred. There was no significant difference in the incidence of adverse reactions between the two groups, and there was no statistical significance. The experimental group of patients with respiratory stabilization time is (1.76±0.32) days, to eliminate irritability (7.12±1.12) hours, heart rate recovery time (1.82±0.74) days, pulmonary rales disappeared time was (8.12±1.41) days. The clinical symptoms of the patients in the control group were significantly longer than those in the experimental group, with statistical difference (P<0.05). Conclusion The effective rate about psychological intervention combined with milrinone on the treatment of the children with congenital heart disease complicated with severe pneumonia and heart failure is high, which is worthy of clinical application.

BACKGROUND:In recent years, cel transplantation therapy is widely used in the treatment of Parkinson’s disease with satisfactory outcomes. OBJECTIVE:To investigate the proliferation and differentiation of transplanted neural stem cel s in Parkinson’s disease rats, thereby providing a new theoretical basis for the treatment of Parkinson’s disease. METHODS:Neural stem cel s from the brain tissues of rats were isolated and cultured in vitro using col agenase digestion method. Unilateral rat model of Parkinson’s disease was made by 6-hydroxydopamine method, and the successful rat models were divided into model group and cel transplantation group, with seven rats in each group. The rats in the model group were given 4 mL normal saline at the damaged striatum, and those in the cel transplantation group were injected 5μL green fluorescent protein-labeled neural stem cel s (1×109/L) into the damaged striatum. RESULTS AND CONCLUSION:After cel transplantation, the positive area of tyrosine hydroxylase was significantly lower in the cel transplantation group than the model group. Compared with the model group, the expression level of Ptx3 mRNA was significantly higher in the cel transplantation group at 7, 14, 28 days after cel transplantation and the expression of SHH mRNA was significantly higher in the cel transplantation group at 14 and 28 days after cel transplantation. However, there was no difference in the expression of Nurrl mRNA between the two groups. These findings indicate that neural stem cel s injected into the damaged striatum of rats can differentiate into dopaminergic neurons, which bring a breakthrough in the treatment of Parkinson’s disease.

Most colorectal cancers develop from adenomas. Environmental factors play an important role in the development and progression of adenoma. Recently,epidemiologic investigations on lifestyle change including diet and exercise and drug intervention studies have proved the relationship between environmental factors and colorectal cancer. Screening,polypectomy and surveillance via colonoscopy are the main measures of colorectal cancer prevention. However, the efficacy is unsatisfactory. Therefore,attention should be paid to studies on colorectal cancer prophylaxis via intervention on environmental factors. Emphasis should be put on various aspects of environmental intervention in clinical practice,and prophylaxis strategies should be generated individually in accordance with the local condition and situation.

Objective To evaluate the efficacy and safety offlupentixol and melitracen (deanxit) in the treatment on the patients with post-stroke depression.Methods A total of 98 patients of post-stroke depression were randomly divided and assigned to a therapy group and a control group.The two groups was given a routine treatment of stroke.The therapy group was treated with deanxit (52 patients,4 weeks).Both groups were measured by Hamilton rating scale of depression (HAMD),China stroke scale (CSS) and Barthel index(BI)before treatment and after 2 weeks and 4 weeks.Results The efficiency rate in treatment group was much higher than that in control group [86.5％ (45/52) vs.43.5％ (20/26),P ＜ 0.01].HAMD score,CSS score and BI in therapy group were better those in control group 4 weeks after treatment [(9.38 ±1.80) scores vs.(14.56 ± 2.40) scores,(15.79 ± 0.83) scores vs.(28.91 ± 1.02) scores,74.6 ± 15.4 vs.59.2 ± 14.6,P ＜ 0.05].There was obvious untoward reaction in therapy group.Conclusions It is indicated that deanxit can reduce the degree of depression and promote the neurological functional rehabilitation.It is safe and effective in the treatment of post-stroke depression.