Objective To investigate the proliferation and the change of antigen on the cord blood CIK cells. Methods Cord blood CIK cells were generated by culture cord blood mononuclear cells in the presence of ?-IFN on day 0 and rhIL-2, antiCD3 MCAb on day 1. CIK cultures were analysed on different time point by FACS(Fluorescence activated cell sorter). The killing efficacy in tumor cell lines and primary leukemia cells were detected by MTT assay. Results After 2~3 weeks of culturing cord blood CIK cells expanded about 64.14?16 folds and CD+3 CD+56 cells which were the major cytolytic effector in CIK cells proliferated about 900 folds. Cord blood CIK cells have great cytotoxity against tumor cell lines as well as primary leukemia cells. Conclusions Cord blood MNCs can be cultured to CIK cells and the times of expansion are very high. Cord Blood CIK cells are highly efficient cytolytic effector cells.

0.05).[Conclusion]Lateral-anterior fixation with use TSRH not only has effective result in strength, rigidity and stability of the spine, but also is superior to the control group in loading-bearing ability and stability of the spine. There is no significant difference between the TSRH group and Z-plate group. But TSRH is relatively simple to operate and is cheaper than Z-plate, and has exact clinical ontcome.

To apply more precise upper airway modeling and computer fluid dynamic technology, and simulate the upper airways of the fluid velocity, flow field distribution and pressure changes on 1 case of OSAHS patients before and after surgery. At home and abroad, computer fluid dynamic has been applied to research on human airway and predict curative effect before and after operation, but the study of the whole upper airway fluid physics parameter is less within normal people and sick people, more the lack of large sample research. The application of this technology can lay the foundation to establish normal people and sick people upper airways database, formulate normative operation and research process, the quantitative domestic normal population and pathological airway blocking criteria of each plane, develop a handling the precision of simulation software and the curative effect of surgical or medical treatment evaluation system, and open up the new train of thought of OSAHS patients diagnosis and treatment.
Airway Obstruction ; Humans ; Hydrodynamics ; Pharynx ; Pressure ; Sleep Apnea, Obstructive ; diagnosis ; therapy ; Software ; Surgery, Computer-Assisted ; Tomography, X-Ray Computed

OBJECTIVE:To provide new ideas for the supervision of TCM decoction for patients. METHODS:The mode of outsourced TCM decoction for patients in Nanjing and Hangzhou area were investigated and analyzed,especially their operation mode. The safety problems of quality of the mode were analyzed from the legitimacy and standardization. RESULTS & CONCLU-SIONS:The present problems include the mismatch of professional and technical personnel of TCM decoction pieces and their qua-lification requirements;the lack of communication of pharmacists and prescribers;no restrictions for outsourced decoction of toxic TCM decoction pieces for patients;the insufficient attention for the quality and safety of packaging materials;being difficult to im-plement the main responsibility of medical institutions,etc. It is suggested to establish the filing system of centralized TCM decoc-tion institution for patients;the stationed system of pharmacy technician in medical institutions;the training and evaluation system of dispensing,decocting and other stations;the regular inspection system of health and pharmaceutical administration department;the quality evaluation system of TCM decoction for patients by following up medical staff and patients. So that it can explore a new idea for monitoring ofclearing the powers of regulatory,full participation in medical institutions,standardization and management of pharmaceutical wholesale enterprises,active supervision by patients.

Objective To study the clinical application of ureteral grasping forceps in ureteroscopic operations. Methods A total of 74 cases treated under ureteroscope by using ureteral grasping forceps from April 2001 to July 2003 in this hospital was retrospectively analyzed. There were 25 cases of polyp resection and pneumatic lithotrity, 21 cases of rigid guidance through ureteral retortion, 19 cases of ureteral stricture dilation and ureteroscopic lithotrity, 3 cases of whole ureteral membranous obstruction, 3 cases of ascending shift of double “J” tube, 2 cases of intraoperative biopsy,4 cases of removal of double “J” tube with urethral stricture,and 2 cases of pneumatic lithotrity. Results The procedures were successfully accomplished in 69 cases.A conversion to open operation was required because of malfunction of ureteral grasping forceps in 1 case, and ureteral perforation occurred in 4 cases. Conclusions Ureteral grasping forceps is indispensable in ureteroscopic operations, which should be skillfully operated.

Objective To investigate the efficacy of paracentesis using jugular vein indwelling catheter (Angiocath) in the treatment of simple renal cysts. Methods We retrospectively analyzed clinical data of 61 patients with simple renal cyst treated by Angiocath paracentesis combined with sclerosing agent injection under the guidance of ultrasonography. Results Fifty-two patients were cured on one session, while a recurrence was seen in 9 patients, among which 6 patients were cured by a re-paracentesis and 3 frequently recurrent patients were cured by the unroofing operation. No complications occurred except for 3 cases of mild transient hematuria. Conclusions Paracentesis using Angiocath in the treatment of simple renal cysts is minimally invasive, safe, effective and economical.

0.05) but was stronger than that under conditions of a and c ( P

Objective To evaluate the surgical methods and the results of treatment for open achilles tendon ruptures.Methods 56 patients with open achilles tendon ruptures were treated by Tsuge stuture or auxileary repair with free deep-fascia circuitously.Results 52 cases had the excellent results,3 case had the middle results,1 case had poor results that marginal skin of the wound were necrosis and subinfection.Conclusion It has a good effect that the open achilles tendon ruptures are treated by modified Tsuge stuture or auxileary repair with free deep-fascia circuitously.

Purpose:To investigate the toxicities and res po nse of liver cancers to 3-dimensional conformal radiation therapy (3-DCRT) com bined with Trancatheter arterial chemoembolization (TACE) Methods :The histopathological confirmed liver cancer patients were treated by 3-DCRT co mbined with TACE, all patients received TACE before 3-DCRT The total irradiati on dose is 50 Gy to 58 Gy in daily fractions of 2 Gy Each planning target volu me (PTV) received a minimum of 90% of the prescribed dose Each mean liver dose not reached 30 Gy, V 30 Gy (the percentage of normal liver volume with ra diation dose≥30 Gy) less than 33% Results:Thirty patients were included in this study including 2 1 hepatocellular carcinoma Survivals at 1 year after 3-DCRT were 76%, with a median survival time of 8 months 2 patients developed Grade 1 acute liver toxi city and one patient experienced Grade 2 gastrointestinal complications No pat ient developed Grade 2 or greater liver toxicity Conclusions:The use of 3-DCRT technique for liver cancer is a safe and efficient method O ur experience indicated the total irradiation dose above 58 Gy is feasible in da ily fractions of 2 Gy, if the mean liver dose did not reach 30 Gy and V 30 G y

Objective To explore the therapeutic efficacy of posterior internal fixation and anterior debride-ment with autogenous bone grafts at one stage on tuberculosis of thoracic or lumbar spine . Methods 16 cases of thoracic or lumbar spinal tuberculosis patients were treated with combined anterior (radical debridement and bone autograft) and posterior (instrumentation) surgeries in one stage between September 2003 and September 2007. The degree of the kyphosis (Cobb angle) was measured and the interbody fusion was observed preoperatively and postop-eratively. The ASIA grading system was used to assess the neurological status. Results All patients were followed up for 10 months to 36 months,on average of 12 months. All patients showed sucessful interbedy fusion,but Cobb angle was not progressed. No recurrence or wound infection was found. 6 cases all got nerve function recovery. Conclusion Thoracic or lumbar tuberculosis treated with this surgical technique can achieve stable internal fixation and a high satisfactory rate with restoring the spinal stability, arresting the disease early, providing early fusion, correcting the ky-pbosis particularly.