1.Expression and significance of FasL and FAP-1 in acute myeloid leukemia
Journal of Leukemia & Lymphoma 2009;18(8):481-483
Objective To explore the expression of the mRNA level of Fas (CD95) ligand/FasL and Fas-associated phosphatase-1/FAP-1 in acute myeloid leukemia. Methods The expression of FasL and FAP-1 were detected in 54 patients with AML and 10 normal subjects by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). β-actin used as internal reference. The changes of FasL was observed after induction chemotherapy in 16 AML patients. The expression of Fas was detected in 54 patients with AML by flow cytometry. Results The mRNA levels of FasL and in 54 patients were remarkably higher(P <0.05) than the normal controls. The rates of first complete remission(CR) were significantly higher in FasL(+) (77.78 %) than in FasL(-) cases(16.67 %) (P<0.01). The FasL level declined in 10 patients with good response to therapy (P<0.01), and high level in 6 nonresponding patients with poor response (P>0.05). The mRNA levels of FAP-1 in 54 patients was remarkly higher than that of the normal control. 8/29 cases in Fas-positive group were positive for FAP-1 mRNA expression. 19/25 cases in the Fas-refractory group didn't express FAP-1 mRNA. Conclusion The expression of FasL was high in AML. The rates of complete remission were high in FasL positive cases. The FasL level declined in patients with good response to therapy. The expression of FAP-1was partly expressed in AML. The expression of FAP-1 was less in Fas positive group.
2.The clinical analysis of 64 patients with poststroke epilepsy
Zhenhua WANG ; Lanying JIN ; Feng GAO
Chinese Journal of Postgraduates of Medicine 2011;34(z2):14-16
Objective To explore the clinical characteristics of poststroke epilepsy and to discuss the association with the type of stroke,the location of focus.Methods Clinical information of 64 patients with poststroke epilepsy taken from 932 patients with stroke were analyzed retrospectively.Results In the patients with first stroke,the incidence of poststroke epilepsy was 6.9%.Among them,47 cases belong to early seizure (73.4%) and 17 cases belong to late seizure (26.6%).The incidence of poststroke epilepsy was difference between ischemic stroke and hemorrhagic stroke.There were more cases of stroke in cortical focus than subcortial one.Onset seizures type was more cases of partal seizure than generalized tonic-seizure (GTS).The treatment on early seizure could be short-range treatment and the treatment on late seizure should be long-term standardized treatment and regular.Conclusions Occurrence of poststroke epilepsy were involved in the type of stroke,the location of focus (cortex / subcortex).It is helpful for guiding the clinical therapy of patients with stroke and improving the quality of their life to analyse the clinical characteristics of poststroke epilepsy.
3.Investigation of sleep quality and mental health status of chronic obstructive pulmonary disease patients with respiratory failure
Jia FENG ; Bifei FANG ; Zhenhua ZHU
Chinese Journal of Primary Medicine and Pharmacy 2017;24(19):2972-2975
Objective To explore sleep quality and mental health status of chronic obstructive pulmonary disease(COPD) patients with respiratory failure.Methods From October 2015 to June 2016,COPD patients with respiratory failure hospitalized in our hospital were selected as study group.Patients without COPD hospitalized in our hospital were selected as the control group.The sleep quality and mental health were compared between the two groups.Results The age and body mass index (BMI) between the two groups showed no significant difference (t =0.753,0.693,all P >0.05).The forced expiratory volume in one second (FEV1%) expected value,arterial oxygen pressure (PaO2),oxygen saturation (SaO2) in the study group were (52.34 ± 12.25) %,(9.21 ± 1.83) mmHg,(90.36 ± 3.47) %,which were significantly lower than those in the control group,the differences were statistically significant (t =25.199,5.414,16.439,all P < 0.01).The somatization,obsessive-compulsive symptoms,interpersonal sensitivity,depression,anxiety,terror between the two groups had significant differences (all P < 0.05),the differences in hostility,paranoia and psychosis were not statistically significant (all P > 0.05).The quality of life scale (GQOL-74) scores in cognitive function [(74.47 ± 6.13) points],psychological function [(71.28 ± 7.36) points],physical function [(73.73-± 8.85) points] and social function [(74.35 ± 7.18) points] in the study group were significantly lower than those in the control group,the differences were statistically significant (t =9.735,13.972,5.739,10.040,all P < 0.01).Conclusion The sleep quality and mental health status of COPD patients with respiratory failure is poorer,we should pay more attention and intervention in clinical diagnosis and treatment.
4.Renal transplantation of 26 cases from living related renal donor
Shaolin ZHANG ; Bochuan CHEN ; Zhenhua FENG
Journal of Chinese Physician 2001;0(09):-
Objective To investigate the efficacy of renal transplantation from living related renal donor.Methods A retrospective analysis of 26 cases of renal transplantation from living related renal donor and 84 cases cadaveric renal transplantation was conducted.Results All the operations in these two groups underwent smoothly.The rate of patient/renal survival was 96.15%/96.15%,96.15%/96.15%,96.15%/92.31% respectively in the group of living related renal transplantation,and 96.43%/95.23%,85.71%/80.95%,65.48%/60.71% respectively in the group of cadaveric renal transplantation 1,3,5 years after the operation.The time of cold blood-deficiency and the time of warm blood-deficiency in the living related renal transplantation group were both shorter than those of the cadaveric renal transplantation group(P
5.Biomechanical study and application of lateral-anterior internal fixation with TSRH in thoracolumbar fracture
Zhenhua JIANG ; Xiangdong CHEN ; Feng ZHANG
Orthopedic Journal of China 2006;0(06):-
0.05).[Conclusion]Lateral-anterior fixation with use TSRH not only has effective result in strength, rigidity and stability of the spine, but also is superior to the control group in loading-bearing ability and stability of the spine. There is no significant difference between the TSRH group and Z-plate group. But TSRH is relatively simple to operate and is cheaper than Z-plate, and has exact clinical ontcome.
6.Design of a system for real-time seizure detection and closed-loop electrical stimulation.
Zhenhua HU ; Zhouyan FENG ; Xiaojing ZHENG ; Ying YU
Journal of Biomedical Engineering 2015;32(1):168-174
In order to investigate the effect of deep brain stimulation on diseases such as epilepsy, we developed a closed-loop electrical stimulation system using LabVIEW virtual instrument environment and NI data acquisition card. The system was used to detect electrical signals of epileptic seizures automatically and to generate electrical stimuli. We designed a novel automatic detection algorithm of epileptic seizures by combining three features of field potentials: the amplitude, slope and coastline index. Experimental results of rat epileptic model in the hippocampal region showed that the system was able to detect epileptic seizures with an accuracy rate 91.3% and false rate 8.0%. Furthermore, the on-line high frequency electrical stimuli showed a suppression effect on seizures. In addition, the system was adaptive and flexible with multiple work modes, such as automatic and manual modes. Moreover, the simple time-domain algorithm of seizure detection guaranteed the real-time feature of the system and provided an easy-to-use equipment for the experiment researches of epilepsy control by electrical stimulation.
Algorithms
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Animals
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Deep Brain Stimulation
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instrumentation
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Disease Models, Animal
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Electroencephalography
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Epilepsy
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diagnosis
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Equipment Design
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Rats
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Seizures
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diagnosis
7.Clinical study of percutaneous liver tumor injection combined with transcatheter arterial chemoembolization in the treatment of advanced hepatocellular carcinoma
Wenyuan CHENG ; Zhenhua DAI ; Lixia LOU ; Yunfeng FENG
Chinese Journal of Primary Medicine and Pharmacy 2015;(15):2247-2249,2250
Objective To study the effects of percutaneous liver tumor injection combined with the clinical efficacy of transcatheter hepatic artery chemoembolization in the treatment of advanced liver cancer,and to provide ref-erence for clinical treatment.Methods 22 patients using percutaneous liver tumor injection combined with transcath-eter arterial chemoembolization for treatment were selected,with which 1 month follow-up after discharge.Situation of patients with percutaneous liver tumor injection and transcatheter hepatic artery chemoembolization was analyzed,and the changes of the patients in the following -up of survival time,tumor volume and clinical symptoms were also ana-lyzed.Results Among the patients of postoperative recheck after 6 weeks,6 cases were complete remission,there were partial remission in 8 cases,6 cases of stable,2 cases of progress.Follow up to 2013 December,the patients'sur-vival time was 17-82 months,the average survival time was (55.71 ±13.47)months.After treatment,4 cases of patients'tumor diameter reduced 1 -3cm,18 cases of tumor diameter reduced 3 -5cm,19 cases of liver area pain symptoms for more than half a year of remission,3 cases of liver area pain relief time less than half a year.During the follow -up period,12 patients died of multiple organ failure.Conclusion The development of percutaneous liver tumor injection combined with transcatheter hepatic arterial chemoembolization therapy can delay the development of the disease in patients with advanced HCC,and prolong the survival time.
8.Experimental Investigation of Correlation Between MSCT Perfusion and Blood Lipid in Steroid-induced Avascular Necrosis of Femoral Head
Yue DONG ; Feng WEN ; Zhenhua WU ; Guoguang FAN
Journal of Practical Radiology 2001;0(06):-
Objective To explore the correlation between change of blood lipid and MSCT perfusion imaging in steroid-induced femoral head necrosis in adult rabbits.Methods 20 rabbits 1~1.5 years old in age were divided into two group:16 rabbits in experimental group,8 mg/kg Methylprednisolone was injected twice a week;4 rabbits were used as control group.At 2,4,6,8 week after injection,4 rabbits in experimental group and 1 rabbit in contral group were killed respectively.Before killing the rabbits,the blood lipid was examined and MSCT perfusion imaging was done and the correlation between them was analysed.The index of TRIG,CHOL,Perfusion,PEI,TTP,MTT.All femoral heads were undergone pathological investigation.Results Two weeks after injection,blood lipid began increasing,then blood flow of femoral head decreasing obviously with prolongation of injection;PEI and MTT decrease only 4 weeks after injection.There were a negaitive correlation between blood lipid and blood flow of femoral head.Conclusion There were obviously correlation between the level of increasing blood lipid and blood flow decreasing in steroid-induced avascular necrosis of femoral head in rabbit.
9.Effect of polysaccharide nucleic acid fraction of bacillus Calmette-Guerin on CD4+CD25+Foxp3+ regulatory T cells in peripheral blood of patients with condyloma acuminatum
Zhenhua WANG ; Fanghong YANG ; Yi SUN ; Hui WANG ; Feng XUE
Chinese Journal of Dermatology 2014;47(9):665-666
Objective To evaluate the effect of polysaccharide nucleic acid fraction of bacillus Calmette-Guerin (BCG-PSN) on peripheral blood CD4+CD25+Foxp3+ regulatory T (Treg) cells in patients with condyloma acuminatum (CA).Methods Forty-two patients with first onset of CA were randomly assigned to receive either injection of BCG-PSN (0.35 mg every other day for 3 months) after fulguration (combination group,26 patients),or fulguration only (fulguration group,16 patients).Venous blood samples were obtained from all the patients at the initial visit and three months after the beginning of treatment,as well as from 30 healthy checkup examinees.The percentage of peripheral Treg cells in CD4+ T lymphocytes was determined by flow cytometry.The recurrence of CA was evaluated during the three months after the beginning of treatment.Results The percentage of peripheral Treg cells in CD4+ T lymphocytes was significantly higher in patients with CA than in the controls (8.31% ± 1.24% vs.5.15% ± 0.72%,P < 0.01),and in patients with clinical recurrence of CA than in those without (9.34% ± 0.72% vs.7.45% ± 0.85%,P < 0.01).The recurrence rate was significantly lower in the combination group than in the fulguration group (30.77% vs.68.75%,P < 0.05).After three months of treatment,the combination group showed lower percentage of Treg cells in CD4+ T cells compared with the fulguration group (5.87% ± 1.05% vs.6.60% ± 0.75%,P < 0.05).Conclusions The percentage of Treg cells has a close relationship with the progression of CA,i.e.,the higher the percentage,the more frequent the relapse.BCG-PSN may enhance the antiviral immune response in patients with CA and improve their prognosis by reducing the number of Treg cells.
10.The feasibility of building the oblique lumbar interbody fusion corridor: A CT image study
Haichao XU ; Zhenhua FENG ; Xiaobin LI ; Aimin WU ; Wenfei NI
Chinese Journal of Orthopaedics 2017;37(16):1021-1028
Objective To determine the radiographic feasibility of oblique lumbar interbody fusion (OLIF) corridor to treat lumbar disease at each lumbar disc level,including the corridor's numerical value and the influence of diaphragmatic crura and aorta abdominalis.Methods A retrospective CT study was conducted on 110 patients (including 69 males and 41 females,average age 47.95 years,range 16-83 years) that continuously collected and analyzed in the PACS system.The oblique corridor was defined as the area between the left lateral border of the aorta abdominalis(or iliac artery) and the right lateral border of the left psoas.The distances and angles of L1-2,L2-3,L3-4 and L4-5 levels were measured.Whether the change of diaphragmatic crura and aorta abdominalis affected the building of the corridor was also observed.Results The mean distances of oblique corridor to the levels of L1-L5 discs were:L1-2 15.90 mm,L2-3 14.82 mm,L3-4 17.57 mm,L4-5 11.16 mm.At the levels of L1-2 and L3-4,all of the images could build the corridor.But there were only 97.27% images allowing operation at both L2-3 and L4-5,and the other 3 cases couldn't build the corridor since the aorta abdominalis was very close to psoas,and the distance was almost 0 mm.The max mean distance was 36.79 mm at L3-4 level.The mean angles were:L1-2 36.98°;L2-3 37.76°;L3-4 40.96°;L4-5 37.85°.The significant difference was at L3-4,ranged from 13.09 to 61.93°.The level of the aortic bifurcation was from the lower third of the L3 vertebral body to the middle third of the L5 vertebral body.The levels of left diaphragmatic crura's ending point in the lumbar was divided into four groups:1) Group L1 vertebral body level:the level at L1 vertebral body and above,5 cases (4.55%);Group L1-2 disc to L2 vertebral body level:at L1-2 disc and L2 vertebral body,67 cases (60.91%);Group L2-3 disc to L3 vertebral body level:at L2-3 disc and L3 vertebral body,36 cases (32.72%);Group L3-4 disc to L4 vertebral body level:at L3-4 disc and L4 vertebral body,2 case (1.81%).Conclusion The OLIF corridor can be built successfully at L1-2 and L3-4.However,it may be difficult at L2-3 and L4-5 for some patients due to the aorta abdominalis which is too close to psoas.The angles of L1-L5 levels were similar.While the left diaphragmatic crura was mainly impact the corridor insertion at L1-2 and L2-3.And the level of the aortic bifurcation was mainly located at the upper endplate of L4 to the L4-5 disc (87%).