Objective To evaluate the effect of montelukast combined with seretide in treatment of patients with asthma and its influence on balance between Th17 and Treg cells, and expression of IL-4 and IFN-γ.Methods 110 patients with asthma were randomly divided into observation group and control group, observation group of 55 cases, using montelukast combined with seretide, control group of 55 cases, using seretide,compared two groups of patients with pulmonary function changes, peripheral inflammatory factor changes and influence on Th17 and Treg cells balance. Results Before treatment, observation group and control group,FEV1 and FEV1 /FVC value was no significant difference.FEV1 and FEV1 /FVC after treatment were significantly improved compared with before treatment in two groups, differences were statistically significance (P<0.05).Between two groups, FEV1 values were significantly difference (t=8.1156, P=0.0001), FEV1 /FVC also had significant difference (t=4.8933, P=0.0001) .Before treatment, observation group and control group, IL-4 and IFN-γdid not have a statistically significant.After treatment, IL-4 and IFN-γdecreased significantly, with statistical difference compared with before treatment (P<0.05).Between two groups, IL-4 had significant difference (t=6.0273, P=0.0001), IFN-γalso had statistically significant (t=6.6042, P=0.0001).Before treatment, there was no significant difference between Th17 and Treg percentage, after 12 weeks of treatment and 24 weeks, Th17 in observation group were(0.53 ±0.02),(0.32 ±0.01), the control group were (0.78 ±0.03),(0.62 ±0.02), which had differences (P<0.05).After 12 weeks and 24 weeks,observation group Treg respectively(2.21 ± 0.22),(2.47 ±0.10),in control group were(1.50 ±0.11),(1.84 ±0.14), Treg percentage at different time after treatment, the differences had statistically significant (P<0.05).Conclusion Montelukast combined with Seretide in treating with asthma, pulmonary function of patients is obviously improved, and it can improve Th17, Treg cell.

Good patient care requires accurate and comparable laboratory test results. To achieve standardization, a reference system consisting of a hierarchal framework of validated and reliable analytical methods linked by reference materials must be in place. Supported by national and municipal research programs, construction of reference systems has been pursued and progress been made. Candidate reference methods have been set up or developed for some important tests, and the methods have been used in the calibration or evaluation of routine methods, study of commutability of control materials and characterization of reference materials. Phenomenal roles of reference systems are expected in diagnostic reagent manufacturing, internal quality assurance and external quality assessments.

specifications are essential for total quality management in laboratory medicine. A consensus among worldwide professionals has been achieved and a hierarchy of strategies for setting analytical quality specifications has been proposed based on their relevance to medical decision-making. Quality specifications derived from biological variations have been widely accepted because of their objectivity and practicability and have more and more been used in the quality management in laboratory medicine.

Objective: To understand the study status of Extended Release Venlafaxine (Venlafaxine ER) in the treatment of depression and its efficacy in China. Methods: 8 papers on Venlafaxine ER treatment of depression were indexed and reviewed with evidence-based medicine method. Results:7 of 8 studies were RCT. The effect size of Venlafaxine ER in the treatment of depression at the end of week 2 was more significant than that of SSRIs (Z=-2.17,P

0.05).[Conclusion]Lateral-anterior fixation with use TSRH not only has effective result in strength, rigidity and stability of the spine, but also is superior to the control group in loading-bearing ability and stability of the spine. There is no significant difference between the TSRH group and Z-plate group. But TSRH is relatively simple to operate and is cheaper than Z-plate, and has exact clinical ontcome.

Since 1974, 39 cases, after thumb reconstruction by means of free transplantation of second toe, haveundergone functional training in the therapeutic gymnasium of Huaskan Hospital. Functional training consists of using intermittent traction with indigenous equipmints to moblize the thumb joints and using resistanceexercises with small rubber balls and wooden framed net of rubber bends to strengthen the thumb musclesThe training was carried out three sessions a week for a total of 18--219 sessions, averaging 49. 5 sessions. The distribution of cases in classes of basic function of the thumb was as follows: Before training: Good, 0; fair, 11; poor, 28; After training: Good, 2; fair. 32; poor, 5. Practical activities of the hand, such as selfserving. writing, sewing and using common tools were perfor-med satisfactorily when basic function of the thumb reached "good" or "fair" class.

Objective: To compare the quality of life of schizophrenics treated with risperidone and clozapine Method: We followed 24 schizophrenics treated with risperidone and 26 with clozapine for 6 months All subjects were assessed with PANSS, TESS and WHO QOL-100 before treatment and 1, 2, 3, 6 months after Results: After 6 month, risperidone and clozapine had similar effects on positive and negative symptoms of schizophrenia Risperidone group had greater improvement in total score of PANSS Risperidone could significantly improve all aspects of quality of life of patients, except Domination While clozapine just improved some parts of that, it had no help to Physics, Psychology and Independence Conclusion:Risperidone is more favorable than clozapine for schizophrenics to return to society

Objective To study effects of cholecystokinin (CCK) on occuring of electrogastric arrhythmias. Methods The contens of cholecystokinin (CCK) nerves, cholinergic (Ach) nerves and nitregic nervous (NOS) nerves in antral myenteric plexus were studied using histochemistry and immunocytochemistry. Results (1) The electrogastric dysrhythmias occurred in the model and CCK groups rats, the abnormal rhythm index and coefficent of variation (cv) of slow wave frequency were singnificantly increased compared with the control ( P

Objective To design siRNA for Caveolin-1(CAV1) and construct eukaryotic expression vector Dsilence4.1-CMV-neo-CAV1, which is transfected into gastric cancer cell line SGC7901 to study the effects of inhibition of CAV1 expression with siRNA on biological behavior of gastric cell line SGC7901.Methods Design and chemosynthesize three pairs of double strands siRNA based on the neucleotide sequence of CAV1 gene provided by GeneBank.The siRNAs were transient transfected into SGC7901.The CAV1 expression of transfected cells were detected respectively by semi-quantity RT-PCR to sieve a valid siRNA sequence.Design and synthesized DNA sequence which can express small hairpin RNA(shRNA),construct RNA interfering eukaryotic expression vector of CAV1 gene.The SGC7901 Was stably transfected and was detected the expression of CAV1 gene by RT-PCR.The influence on the gastric cell growth and pmliferation after CAV1 was inhibited Was detected by cell growth curve, clone formation test and FCM.Results The CAV1 RNA interfering eukaryotic expression vector psilence4.1-CMV-neo-CAV1 was successfully constructed,which can significantly reduce the expression of CAV1 after stably transfected to target cells,and in contrast to Dsilence4.1-CMV-neo transfection group,PI and colony for mution rate were increased(t=7.98,P<0.05;t=13.19,P<0.01),the growth speed Was up.Conclusion The growth and proliferation ability of tumor is remarkablv accelerated by inhibiting the expression of CAV1 of gastric cell by RNAi.

Objective:By analyzing clinical data, discuss the clinical efficacy of balloon dilatation for the treatment of tuberculous scarred airway stenosis and factors affecting efficacy. Methods:Selected 13 cases airway stenosis caused by tracheobronchial tuberculosis.Airway stenosis was confirmed and measured by bronchoscopy and CT before balloon dilation. Balloon dilatation with forming expansion was conducted once a week. We evaluated the shortness of breath scores, measuring FEV1, FVC and the diameter of airway before and after dilation. The long-term outcome and lung function improvement were evaluated. Results:Thirteen cases were treated by balloon dilation with fiberoptic bronchoscopy. The airway diameter before and after the treatment, shortness of breath score, FEV1, FVC have improved significantly, the difference was significant(t=15.596, t=-27.657, t=-14.604, t=-41.766;P<0.05). Conclusion:Balloon dilation treatment for tuberculous scarred airway stenosis can achieve better results.