Objective To evaluate the clinical effects of percutaneous kyphoplasty ( PKP ) for osteoporosis vertebral compression fractures . Methods From January 2011 to June 2013, 65 patients underwent PKP .The evaluation was based on the visual analogue scale (VAS), vertebral height, and Cobb’s angle recorded at 3 days and 6 moths postoperatively.The duration of follow-up after operation was 6-12 months. Results The PKP operation was successfully completed in all the 65 cases.The VAS reduced from 6.62 ±0.63 before operation to 3.22 ±1.20 at 3 days (P =0.000) and 2.12 ±1.15 (P =0.000) at 6 months postoperatively.The vertebral height was increased from (15.26 ±1.19) mm before operation to (18.14 ±1.29) mm at 3 days (P=0.000) and (17.65 ±1.37) mm (P=0.000) at 6 months postoperatively.The Cobb’s angle was decreased from 16.25°±2.66° before operation to 6.34°±1.68°at 3 days (P=0.000) and 7.13°±1.82°(P=0.000) at 6 months postoperatively. Conclusion Percutaneous kyphoplasty can relieve pain and improve the functions .

Objective To study the reproductive health status in adolescents underwent repeated abortion and their psychosocial behavioral status and to understand their demanding on the knowledge of contraception, so as to provide suggestions on post abortion care. Methods From July ,2007 to May,2008,400 unmarried adolescents who visited doctors and met the following criteria were recruited in the study:①Younger than 24 years old;②Had artificial abortion at least once;③Have been pregnant for 5-10 weeks and asked for artificial abortion. Data were collected through symptom checklist 90 (SCL-90)and anonymous reproductive health questionnaire. The situation of reproductive tract infections (RTIs) were detected through gynecological examination and laboratory tests.The data was analyzed by SPSS 11.5 statistical software. Results The mean age of the subjects was 21. 18 ±2. 46 years. The mean time of previous artificial abortion was 1.96 ± 1.44,with 52% of ≥2 times. Aged less than 20 years old at the first abortion,having sex more than 3 times per week,more than 2 sex partners,and history of painless abortion were associated with repeated abortion in unmarried adolescents. The SCL-90 scores showed that the subjects had higher score of depression, terror, psychiatric symptoms, somatization, interpersonal sensitivity,anxiety and hostility factors than normal population (P < 0. 05). Of the subjects ,34. 5% had history of RTIs, and the current prevalence of RTI was 59. 5% ,and 58. 5% with one kind of RTI and 36. 0% with two kinds of RTI. Of these RTI patients,33. 5% did not present any clinical symptoms. Abortion for more than twice,first sex intercourse occurred at age younger than 18,history of RTI,sex intercourse of more than 3 time per week,multiple sex partners,especially18,history of RTI,sex intercourse of more than 3 time per week,multiple sex partners,especiallyhaving multi sex partner at the same, and poor sex sanitation were risk factors of RTI. Conclusions There are various reproductive health problems among repeated abortion adolescents in mental and physical aspects, lack of knowledge on reproductive health and contraception is the main reason. Improving post-abortion services will be helpful to prevent repeated abortion in adolescents and protect their physical and mental health.

AIM:To investigate the effects of P2X4 receptor on peri-sciatic administration of recombinant rat TNF-α(rrTNF)-induced mechanical allodynia.METHODS:Male Sprague-Dawley rats (180~200 g) were used in the experiments.The levels of P2X4 receptor on day 3, day 7 and day 14 after peri-sciatic administration of rrTNF were exam-ined by Western blot, and the location of P2X4 receptor in the spinal dorsal horn was observed by double immunofluores-cence staining.The changes of 50%paw-withdrawal thresholds of the rat were detected by behavioral test, and the level of TNF-αin the spinal dorsal horn was also examined by Western blot when TNP-ATP was intrathecally injected before the ad-ministration of rrTNF.RESULTS:Compared with control group, the expression of P2X4 receptor in the spinal dorsal horn on the ipsilateral side significantly increased on day 3, day 7 and day 14 (P<0.01) after rrTNF (100 ng/L) administra-tion.P2X4 receptor was co-localized only with microglia, but not with neurons or astrocytes.Intrathecal injection of TNP-ATP before rrTNF administration prevented mechanical allodynia induced by rrTNF and inhibited the upregulation of TNF-αin the spinal dorsal horn.CONCLUSION:P2X4 receptors in microglia may be involved in rrTNF-induced mechanical allodynia by the upregulation of TNF-αin the spinal dorsal horn.

BACKGROUND:The smal intestinal submucosa has good biocompatibility and biodegradability, and also contains a variety of growth factors that can significantly promote celladhesion, proliferation and differentiation. Currently, the smal intestinal submucosa has been widely used in bone and cartilage, blood vessels, skin, bladder, smooth muscle and pancreatic tissue repair, showing good performance as a tissue-engineered cellscaffold.
OBJECTIVE:To investigate the in vitro feasibility of tissue engineered periosteum constructed by porcine smal intestinal submucosa and osteoblasts differentiated from bone marrow mesenchymal stem cells.
METHODS:Bone marrow mesenchymal stem cells were harvested from 2-week-old healthy New Zealand rabbits by using adherent method, and then cells were cultured, induced, differentiated and identified in vitro. Fol owing induced differentiation and identification, the bone marrow mesenchymal stem cells were compounded with porcine smal intestinal submucosa to fabricate tissue engineered periosteum. The adhesion, growth, and proliferation of cells on the materials were observed.
RESULTS AND CONCLUSION:At 5 days after inoculation, the cells receiving osteogenic induction could quickly adhere and proliferate on the surface of porcine smal intestinal submucosa and be interconnected;at 10 days, the desmosomes formed among the cells, cellprocesses from osteoblasts were visible and attached to the smal intestine submucosa;at 15 days, cellproliferation and secretion of matrix appeared, and multi-layer membrane-like structure formed on the surface of the smal intestine submucosa. These findings indicate that after osteogenic induction, the bone marrow mesenchymal stem cells can be combined with porcine smal intestinal submucosa to construct a tissue engineered periosteum, which is hoped to be an ideal scaffold for tissue engineering.

Schizophrenia and obsessive-compulsive disorder are common disorders in clinical psychiatry， accompanied by varying degrees of painful experience and social functioning impairment， and a high prevalence rate of co-morbidities. In this paper， a review of comparative study of structural and functional magnetic resonance imaging of the two diseases is presented， so as to explore the similar and specific biological markers of them and to provide a forceful imaging basis for subsequent studies.