In order to preprocess mammograms for diagnosing the early cases of breast cancer and improving the computational efficiency in the computer-aided detection of micro-calcifications in mammograms, we have advanced a novel processing technique for the extraction of micro-calcification region of interest (MROI). The proposed method is based on a three-step procedure: (1) the mammogram is divided into sub-images of the same size; (2) the wavelet multi-resolution method is conducted on the sub-images, and the parameters related to wavelet transform and threshold T are discussed according to rho; (3) the classification of sub-images is determined by T. It is tested with 20 mammograms and the results show that the method can achieve a true positive rate as high as 89.7% with a false positive rate as low as 2.1%.
Breast Diseases ; diagnostic imaging ; pathology ; Breast Neoplasms ; diagnostic imaging ; pathology ; Calcinosis ; diagnostic imaging ; Diagnosis, Computer-Assisted ; Humans ; Mammography

OBJECTIVETo study the distribution and quantity of CD44+/CD24- cells in breast cancer tissue and the cell lines, and as well as its correlation with the expression of various breast cancer markers and molecular subtyping of breast carcinoma.

METHODSThe expression of CD44/CD24, estrogen receptor, progesterone receptor, HER2, human estrogen-induced protein PS2, bcl-2 and nm23 in 60 cases of invasive ductal carcinoma of breast were studied by either single or double immunohistochemical staining. The co-expression of CD44 and CD24 in 3 breast cancer cell lines (MCF-7, MDA-MB-468, and MDA-MB-231) was also examined.

RESULTSThe quantity and distribution of CD44+/CD24- cells varied greatly and no specific patterns were identified. The percentage of CD44+/CD24- in breast cancer was 65%. The amount of CD44+/CD24- cells did not correlate with the age of patients, lymph node metastasis, tumor size, molecular subtypes and expression of various breast cancer markers in breast carcinoma. The proportion of CD44+/CD24- cells in MCF-7, MDA-MB-468, and MDA-MB-231 cell lines was <1%, 5% and >80%, respectively.

CONCLUSIONSCD44+/CD24- cells are demonstrated in certain breast cancer tissues and cell lines. However, there is no relationship obtained between the quantity or the distribution of these cells and the molecular subtyping or the clinicopathologic parameters in breast cancer.

Adult ; Aged ; Biomarkers, Tumor ; Breast Neoplasms ; classification ; metabolism ; pathology ; CD24 Antigen ; metabolism ; Carcinoma, Ductal, Breast ; classification ; metabolism ; pathology ; Cell Line, Tumor ; Female ; Humans ; Hyaluronan Receptors ; metabolism ; Lymphatic Metastasis ; Middle Aged ; NM23 Nucleoside Diphosphate Kinases ; metabolism ; Proto-Oncogene Proteins c-bcl-2 ; metabolism ; Receptor, ErbB-2 ; metabolism ; Receptors, Progesterone ; metabolism ; Trefoil Factor-1 ; Tumor Suppressor Proteins ; metabolism

Objective To analyze the clinical efficacy of rosuvastatin in the treatment of patients with acute coronary syndrome complicated with arrhythmia and its influence on serum inflammatory factors.Methods A total of 96 patients with acute coronary syndromes complicated with arrhythmia were selected and randomly divided into observation group and control group,48 cases in each group.The patients in the control group were treated with lovastatin,while the patients in the observation group were treated with rosuvastatin.The levels of inflammatory factors,apoptosis and drug-induced adverse reactions were compared between the two groups.Results After treatment,the levels of IL-18,TNF-α,hs-CRP and C-myc,Bcl-2 protein improved significantly in both groups,and these indexes in observation group were significantly better than control group (P ＜ 0.05).There was no significant difference in incidence rate of adverse reactions between the two groups (P ＞0.05).Conclusion Rosuvastatin is effective and safe in the treatment of patients with acute coronary syndrome complicated with arrhythmia.

Objective To analyze the clinical efficacy of rosuvastatin in the treatment of patients with acute coronary syndrome complicated with arrhythmia and its influence on serum inflammatory factors.Methods A total of 96 patients with acute coronary syndromes complicated with arrhythmia were selected and randomly divided into observation group and control group,48 cases in each group.The patients in the control group were treated with lovastatin,while the patients in the observation group were treated with rosuvastatin.The levels of inflammatory factors,apoptosis and drug-induced adverse reactions were compared between the two groups.Results After treatment,the levels of IL-18,TNF-α,hs-CRP and C-myc,Bcl-2 protein improved significantly in both groups,and these indexes in observation group were significantly better than control group (P ＜ 0.05).There was no significant difference in incidence rate of adverse reactions between the two groups (P ＞0.05).Conclusion Rosuvastatin is effective and safe in the treatment of patients with acute coronary syndrome complicated with arrhythmia.