Objective To study the effect of ShenQi FuZheng injection on side effects of chemotherapy and the patients' life quality after operation of colon cancer. Methods 60 patients were recruited into a control group and a treatment group, with 30 patients in each group. The treatment group was treated with conventional chemo-medicines plus ShenQi FuZheng injection, while the control group was treated with conventional chemo-medicines exclusively. Side effects and life quality were compared between the two groups after two courses of treatment. Results The side effects and mark of the life quality in the treatment group were both obviously better than the control group (P<0.05) after the treatment. Condusion ShenQi FuZheng injection can decrease side effects of chemotherapy and improve patients' life quality.

Objective:To investigate the changes of neuron-specific enolase(NSE)concentration in serum and cerebrospinal fluid from patients with acute and recovery viral encephalitis and to find out its correlation with anomaly electroencephalogram and low density focus volume in imageology. Methods:42 cases of patients with viral encephalitis were enrolled and 25 cases of health examinater or patients with operation of appendix or cholecyst were enrolled in control group.We measured the NSE concentration in cerebrospinal fluid and serum using enzyme-linked immunosorbent assay. Comparison analysis was performed by using a SPSS13.0 statistical package. Results:NSE concentration in serum and cerebrospinal fluid of patients with acute viral encephalitis was significantly higher than that of recoverys and control group(P

Objective:To evaluate the clinical value of determinations of C-reactive protien concentration in serum and cerebrospinal fluid from patients with acute and recovery cerebral infarction and to find out the correlation of it with neurologic deficits,infarct sige and duration.Methods:46 cases of patients with cerebral infarction were recruited as observe group and 25 cases of healthy examinees or patients undergoing operation of fracture,appendix or cholecyst ectomy were recruited as control group.We measured C-reactive protien in cerebrospinal fluid and serum.Comparison analysis was performed using a SPSS10.0 statistical package.Results:Concentration of C-reactive protien in cerebrospinal fluid and serum was significantly higher in patients with cerebral infarction than that in patients at recovery stage and control group(P

[Objective]To investigate the effect of Zhenghai biological membrane in the prevention of adhesion of tendon. [Method]Eighteen chicken were divided into 2 groups.Zhenghai biological membrane was used in the experimental group,while simple Kessler suture was applied in the control group.The tendon adhesion degrees were compared between 2 groups with the methods of gross observation,adhesion criterion,histological observation and electron microscopy,et al.[Result]The adhesion degree was lower significantly in experimental group than that in control group.[Conclusion]Zhenghai biological membrane could prevent tendon adhesion effectively,without any obvious rejection reaction.It would not affect the healing of the tendon.

Objective To analyze the reaction in rabbit's esophagus after inserting metallic nitinal stent and obtaining suggestion for the clinical application.Methods The selfexpanding nitinal stent was placed in each of eight rabbits's esophagus. The rabbits were killed at 2,4,6,8 weeks after implantation. Tissue around the stent was investigated with naked eye and microscopy. Results Chronic inflammation and edema were seen in the submucous tissue 2 week after stenting. Slight stenosis occurred at the regions of two ends of the stent and scattered vegetations like polyp were seen with naked eye at 4 weeks, with inflammatory cell infiltration, partial mucous polypoid hyperplasia under microscopy. The pathological changes were more obvious at 6 weeks comparing with the 4 weeks. The lower end of one stent was embedded in esophageal wall. Two rabbits of 8 weeks' group died of obstruction with food tarried inside the stents.Conclusions The restenosis after stenting were mainly seen at the region of terminals of stent. Thus reformation of stent should be taken place urgently especially at the terminals for the prevention of restenosis.

Objective To discuss the differential diagnosis and treatment of bursitis around the knee. Methods 32 patients with bursitis around the knee were treated from March 2000 to March 2002 in our hospital. Their diagnosis and treatment were reviewed. The arthroscopy of knee confirmed all the initial diagnosis of the patients made by the physical examination and complaints. We gave them conservative treatment and analyzed the clinical follow ups. Results All the patients were followed up for 8 to 40 weeks. 9 of them who had no internal knee lesions but only one kind of bursitis healed within 3 weeks. 23 of them who had more than two kinds of bursitis (including four who had internal knee lesions) healed within 3 to 5 weeks. Conclusions The differential diagnosis of the bursitis around the knee can be made on the basis of complaints and physical examination. These cases can be managed by conservative treatment.

0 05) Conclusions Mifepristone with misoprostol for terminating early pnegnaney might be affect the expression of lymphocyte and cytokines and induce the disorder of decidual micro environment which might be the reason of medical abortion

Objective To investigate the prevalence of Borna disease virus (BDV) in viral encephalitis patients in Ningxia Hui Autonomous Region and to analyze phylogenetic development of BDV in this area. Methods The gene fragments of BDV were amplified by real time nested reverse transcriptase polymerase chain reaction (PCR) from peripheral blood mononuclear cells (PBMC) of 60viral encephalitis (VE) patients. Enzyme linked immunosorbent assay (ELISA) was applied to detect p40 antibody in the cerebrospinal fluid from those patients who were confirmed as BDV p24 positive in previous study and those patients who were detected as BDV positive by PCR in the present study.The confirmed p40 positive samples were used for BDV gene cloning and sequencing, which were subsequently compared with the sequences of overseas standard strains, including He/80, H1766 and strain V by software MEGA and DnaSP4.0. The virus phylogenetic tree was constructed based on the above data. Results The positive rate of BDV p24 by PCR was 10. 08% (12/119) and the positive rate of BDV p40 by PCR was 5.88% (7/119). The cerebrospinal samples from two patients were detected as nucleoprotein antibody positive by ELISA, which resulted in a positive rate of 1.68%. The results of gene sequence analysis showed that the sequences of 6 cases among the 7 BDV positive patients were exactly same as the sequence of German horse-origin He/80 strain. Only one synonymous mutation was detected in the remaining one case. Reconstructed gene phylogenetic tree showed a hybrid China (Ningxia)- German and Japan branch was formed by the BDV stain isolated from VE patients in Ningxia and the overseas BDV standard stains. Conclusions BDV infection may exist in VE patients in Ningxia Hui Autonomous Region, which may be associated with close contact between human and animals. The gene sequence of stains isolated from patients in Ningxia is highly homological with the overseas standard strain, which suggests that the domestic BDV may come from overseas, while domestic strains with mutations couldn't be excluded.

This paper was to observe the revaccination effect of domestic yeast-derived recombinant hepatitis B vaccine (YRHBV) to children who were born to HBsAg and HBeAg negative mothers and had been immunized with full course of blood-derived hepatitis B vaccine 4 years ago at their birth. The treated group was revaccinated with 5μg YRHBV while the control group with 10μg blood-derived HB vaccine, their anti-HBs response was determined 1,12,24 months after revaccination. The results showed that a nice anti-HBs response was discovered in group A childeren, no matter how about the anti-HBs level before revaccination was. The kinetics of anti-HBs after YDHBV revaccination was similar to that of the blood-derived HB vaccine, no statistical difference was found. So, 5μg of domestic yeast-derived recombinant HB vaccine gave same immune effect as well as 10μg blood-derived HB vaccine.

Objective To investigate the expression and location of Borna disease virus(BDV)nucleoprotein in transfected oligodendrocytes,and then to explore its expression mechanisms in oligodendroglial cell(OL)and their effects on cell proliferation.Methods We used PCR to detect BDVp40 gene fragments in transfected OL,laser confocal microscopy and Western blot method to detect the expression of nuclear protein and its intracellular location in the cell,MTT to detect the influence of cell proliferation by nuclear protein on the OL.Results We had detected the BDVp40 gene fragments in transfected oligodendrocytes.The nucleoprotein can be positioned in the cytoplasm and cell membrane by laser scanning confocal microscope;Western blot results showed that there is nucleoprotein in the constitutive protein,but was not detected in the cytoplasm.MTT tests showed that nucleoprotein expressed in OL can inhibit cell proliferation.Conclusion It is indicated that the transfected OL stablely express of BDV nucleoprotein,and itslocation is in the cell structure of proteins,particularly in the cytoplasm and more richer in cell membrane,that indicating they may play a key role in cell signal transduction or into the cell-mediated viral infection.BDV nucleoprotein inhibit proliferation of OL,which maybe an important mechanism of Borna disease virus persistant infection and produce symptoms.