OBJECTIVE:To observe the effects of Vitamin B6 on efficacy and related indexes of patients with peripartum car-diomyopathy(PPCM). METHODS:64 PPCM patients were randomly divided into control group(32 cases)and observation group (32 cases). Control group received diuretic drugs,angiotensin converting enzyme inhibitors or angiotensin Ⅱ receptor antagonist,β-blockers,digitalis,low molecular weight heparin and other conventional treatment. Observation group additionally received Vita-min B6 tablet 50 mg,orally,twice a day. They treated for 2 weeks. Clinical efficacy,serum prolactin(PRL),brain natriuretic pep-tide (BNP),left ventricular end-diastolic diameter (LVEDD) before and after treatment,milk back rate and the incidence of ad-verse reactions in 2 groups were observed. RESULTS:There was no significant difference in the total effective rate in 2 groups (P>0.05). Before treatment,there were no significant differences in PRL,BNP and LVEDD in 2 groups (P>0.05). After treat-ment,PRL,BNP and LVEDD in 2 groups were significantly lower than before,and observation group were lower than control group,with statistical significances (P<0.05). And the milk back rate in observation group was significantly higher than control group,with statistical significance(P<0.05). There were no obvious severe adverse reactions during treatment. CONCLUSIONS:Based on conventional treatment,Vitamin B6 shows similar efficacy and safety with conventional treatment in the treatment of PPCM,though it is superior to conventional treatment in terms of decreasing PRL level,it has high milk back rate.

Objective] To explore the value of generation between metal and water in treatment bronchial asthma. [Methods] To analyse the theory of generation between metal and water,and explore the TCM mechanism of asthma through studying the Chinese medical book. Retrieve the relative data about using the method of generation between metal and water to treat asthma. Analyze the application value of generation between metal and water from different treatments to asthma. [Results] The method of generation between metal and water is a therapeutic principle which builds according to the five elements relationship between lung and kidney. It has important guiding significance for qi deficiency ,yin deficiency and yang deficiency of lung and kidney. Asthma’s main pathogenesis is “excess in the lungs,deficiency in kidney”,treated by the method of generation between metal and water can strengthen the body resistance and reduce the frequency of asthma. [Conclusion] Generation between metal and water is a method of lung-kidney concurrent treatment,which can treat both the incidental and fundamental aspects of asthma,make asthma in stable state,and improve the patients quality of life,so it has important value in the treatment of asthma.

Objective To detect mutations in the STK-11 gene in a pedigree with Peutz-Jeghers syndrome (PJS).Methods Blood samples were collected from a 19-year-old male patient with PJS and his unaffected mother,as well as from 100 unrelated healthy human controls.PCR was performed to amplify all the exons of the STK-11 gene followed by sequencing.Results A novel heterozygous missense mutation (G-to-T transition) was identified at position 1251 in the exon 9 of the STK-11 gene in the patient,but not in his mother or the unrelated healthy human controls.Conclusions The missense mutation A417S,which may affect gene transcription and translation,is a specific novel mutation of STK-11 gene.

Objective To investigate the clinical features and changes of the patients with colorectal polyps in the recent 5 years of the Central Hospital of Jiangjin district.Methods Inpatients and outpatients with colorectal polyps found by colonoscopy were collected from January 2011 to December 2015 in Central Hospital of of Jiangjin district.Index of each patient's gender,age,the location,size,number and pathological results of each enrolled.Then the data were analyzed statistically.Results Patients with colorectal polyps in the ratio of male to female was 1.56:1(P＜0.05),middle and old aged group multiple (compared with the young group,P＜0.05);left half colon accounted for 53.0 ％ (P＜0.05),48.0 ％ of them were single shot,diameter≤ 1 cm accounted for most.Adenomatous polyp accounted for 71.0％,most of them were tubular adenoma (54.0％).29.9％ of patients with colorectal polyps had abdominal pain,29.0 ％ changed in bowel habits and traits,only 6.7 ％ of the patients had typical hematochezia.Conclusion According to the anlysis results there's no obvious changes happened on the clinical features of colorectal polyps in the past 5 years.

OBJECTIVE:To develop a RP-HPLC method for the determination of salicylic acid in serum and to apply this method to the pharmacokinetic and bioavailability study of salycylic acid in compound aspirin preparations.METHODS:Waters2690HPLC instrument was used with Diamonsil C 18 column(5?m,250mm?4.6mm)as stationary phase and methanol-ace?tonitrile-0.2%phosphoric acid(18∶32∶50)as mobile phase at a flow rate of1.0ml/min,and the detective wavelength was237nm.RESULTS:Calibration curve of salicylic acid was linear in the range of0.40～101.00?g/ml(r=0.9999).CONCLUS_ ION:The method is simple,sensitive,rapid and suitable for pharmacokinetic and bioavailability study of salicylic acid.

Objective Objective To study the feasibility of direct applying MRI to TPS by measuring the geometric distortion of MRI. Methods Phantom 1 was made by marking radical arrangement of fat pipelines every 15? in foam phantom and Phantom 2 by net arrangement at every 2.7?cm. Coronal and transverse scan parallel with the radial and net fat pipeline in the phantom was performed at sequence of cranial FSE T2WI by Marconi 1.5T superconduct MRI machine. Geometric distortion in 5 different fields of view (FOV) was calculated by measuring the absolute borderline error of direct scan and coronal digital reconstructed radiograph (DRR). Results When FOV was equal or smaller than 11.0?cm?11.0?cm, 22.0?cm?22.0?cm and 27.5?cm?27.5?cm, the geometric distortion was 0.0, 1.1 and 1.4?mm of direct scanning MRI and 1.0,1.4 and 2.0?mm of coronal DRR. Conclusion When the magnetic field of MR is high and the tissue shows uniform density, MRI can be applied on TPS directly.

BACKGROUND: Both MRI and transcranial Doppler can be used for ultra-early assessment of acute cerebral infarction (ACI), the former is considered as a reliable diagnostic means mainly for the morphological changes of ACI, however the latter is often used before obvious morphological changes appeared, but their combined diagnostic value is still uncertain. OBJECrIVE: To investigate the value of combined MRI and transcranial Doppler in ultra-early assessment of highly suspected ACI.DESIGN: Case analysis and double-blind design.SETTING: MRI Department of Daqing Oil-field General Hospital and Department of Clinical Epidemiology of West China School, and Medical-Economic Department of Public Health College, Sichuan University.PARTICIPANTS: From May 2000 to July 2001, totally 225 highly suspected ACI patients with stoke-like onset, 121 males and 104 females,aged of 33-86 years old with the means of (62±12), within 12 hours after onset, informed of the experiment, were selected from Daqing Oil-field General Hospital.METHODS: All cases subjected to transcranial Dopller examination and following MRI examination with interval of no more than 1 hour, then received follow-up MRI examination at 15 days later (as golden diagnostic standards for CI). MRI was performed to detect the position, morphology,size and signal of pathological changes. Transcranial Doppler was used to determine the blood flow velocity, peak value of systolic and diastolic stage, frequency spectrum and sound spectrum of blood flow of bilateral cerebral anterior, middle and posterior arteries, internal carotid artery and basal vertebral artery.MAIN OUTCOME MEASURES: The sensitivity, specificity and accuracy in the diagnosis of ACI with MRI, transcranial Doppler and the combination of MRI and transcranial Doppler.RESULTS: Totally 225 cases were selected, and 5 excluded for not accordant to the inclusion standards, finally 220 statistical analyzed. The diagnostic sensitivity of MRI was 79.3%,. specificity of 98.4% and accuracy of 90.5%, comparing to corresponding 80.4%, 81.3% and 80.9% of transcranial Doppler, and 96.7%, 80.5%, 87.3% of the combination of MRI and transcranial Doppler.CONCLUSION: The combination of MRI and transcranial Doppler can be used to make rapid and accurate assessment of ACI due to higher sensitivity and specificity.

Objective To investigate the expression and prognostic significance of CD151, c-Met and integrin alpha 3, alpha6 in pancreatic ductal adenocarcinoma (PDAC). Methods The expression of CD151, c-Met and integrin alpha3, alpha6 in 71 patients with PDAC and 10 samples of normal pancreas tissues were detected by immunohistochemistry, and the relationship between the expression of CD151, c-Met and integrin alpha 3, alpha 6 and the clinicopathological features, prognosis of these patients was analyzed. Results The positive expression rates of CD151, c-Met and integrin alpha 3, alpha 6 in PDAC were 81.69% (58/71) , 69.01% (49/71), 69.01% (49/71) and 84.51% (60/71) , and there was no expression in normal pancreas tissues. The expressions of CD151, c-Met were significantly associated with TNM stage and lymph node metastasis (P < 0.05). The expression of CD151 was positively correlated with the expressions of c-Met and integrin alpha3, alpha6 (r =0.583, P =0.000, r = 0.457;P =0.000, r = 0.671 ;P =0.000). Univariate analysis suggested the expression of CD151, c-Met and integrin alpha3, alpha6 was associated with survival (P<0.05). Multivariate analysis suggested the expression of CD151, c-Met was the independent prognostic factor for post-operative survival. Conclusions CD151, c-Met and integrin alpha3, alpha6 play a role in the development, metastasis and prognosis of PDAC, and they might be new markers to predict biological behavior and the prognosis of PDAC patients.

In this article, an HPLC method for the contents determination of amino acids in Qihong Maitong injection was reported. In detailed, OPA-Fmoc pre-column derivatization was adopted, and related HPLC methods to determine the contents of amino acids was established. Linear relationship was well constructed for 17 amino acids through the method mentioned above. Briefly speaking, the optimized method was accurate and reproducible, and suitable for the determination of amino acids in Qihong Maitong injection and corresponding quality control.

Objective To observe the curative effect of repairing the distal foot skin and soft tissue defect by using flap.Methods We used nutrient artery flap and low external ankle to repair the foot distal skin soft tissue defect in 16 cases.Results The flap survived,necrosis happened in small skin edge part and distal skin flap of sural nerve nutrient artery flap in two cases,and the skin graft healing with dots after removed the necrotic tissue and changed dressing.16 cases were followed up for 3-16 months,2 cases of flap slightly bloated,1 case back to the hospital got thin skin flap repairing technique;14 cases of walking were as usual,2 lame cases,related to some tissue defect in patients with forefoot injury.The use of flap to repair the foot injury,could maximially recover the limb function,reduce the rate of the sick.Conclusion Skin flap is a kind of operation which is simple,with smaller cost for skin area,beautiful and effective to repair skin and soft tissue defect of foot.