Diffusion tensor imaging (DTI) and fiber tracking (FT) may show structural information about white matter fiber. With development of technology, the applications of the DTI and FT are widely used in spinal cord. DTI and FT are more sensitive than traditional MRI on detecting spinal cord lesions, able to provide more evidence for the diagnosis and differential diagnosis before treatment, as well as the evaluation for the prognosis.

ObjectiveTo analyze the relationship of depression after stroke with the location of lesion and the leve l of central nervous system (CNS) damage, and evaluate the treatment effect.Methods86 stroke patients were evaluated by Zung's self-rating depression scale (SDS). If SDS score ≥50 points, the patient was s elected as subjects for this study and treated with fluoxetine. The relationship of depression with the location of lesion and the level of CNS damage, and the treatment effect were analyzed.ResultsThere were 3 4 cases evaluated as depression (with 27 light depressive cases, 7 moderate depr essive and severe depressive cases). The symptom of depression has a positive co rrelation to the damage level (P<0.05). The depression inci dence of patients with acute sub-cortex stroke is higher than that of cerebral cortex and cerebellum stroke (P<0.01). Fluoxetine has a goo d effect on depression after stroke.Conclusions Th e symptom of depression after stroke has a positive correlation to the damage le vel. Fluoxetine has a good effect on depression after stroke.

Acupuncture Therapy ; Adult ; Erectile Dysfunction ; therapy ; Humans ; Male

Objective To evaluate the application of embolic material, iron oxide nanoparticles?lipiodol?gelatin in the improvement of therapeutic effect of microwave ablation in vitrogelatin phantom model and in vivo VX2 renal tumors model. Methods After preparing new embolic material containing nano Fe2O3, in vitro model experiment was firstly conducted to verify the temperature distribution of the microwave ablation. Further verification was performed in bilateral renal VX2 tumors in rabbit model. A total of 10 (20 kidneys) New Zealand White rabbits bearing renal VX2 tumors were treated by renal artery embolization and CT guided microwave ablation, in which left kidneys were treated by material containing nano Fe2O3 in iodized oil and right kidneys were treated by renal embolization with super liquid iodized oil as a control. The same microwave ablation time and power were used for both kidneys. CT perfusion and histopathological examinations were performed to determine the renal perfusion parameters, tumor histopathology and tumor?related angiogenesis. Results In vitro experiments, when nano Fe2O3 was present in the local area at 1cm from the microwave needle at 20 and 60, the temperature reached(59.1 ± 1.7)° C, (95.5 ± 4.5)° C vs.(31.1 ± 2.5)and(45.6 ± 3.5)° C in the controls. Our present results strongly suggested that local nano Fe2O3 particles absorbed microwave and shielded microwave energy transferring to the surrounding tissue. Before and immediately after renal VX2 tumor embolization, blood flow significantly reduced from (31.4 ± 10.6) to (6.1 ± 9.9) ml · 100 ml-1 · min-1. Our pathology findings indicated nano Fe2O3?lipiodol-gelatin embolic agent stored in endovascular regions, while no penetration into the renal parenchyma happened. Conclusions This in vitro model and in vivo experiment suggest that the new embolic material containing nano Fe2O3 may help to improve heat efficiency of MWA microwave, and it is feasible to administer it through transcatheter arterial embolization approach for the microwave ablation therapy of kidney cancer.

Objective:To investigate the effects of U0126 of different doses on the ability of low frequency magnetic stimulation promoting astrocyte migration and to select suitable dose of U0126.Method:Twenty-four adult healthy SD rats were selected to receive Injection of 0.5ml of 1% ethidium bromide(EB) in PBS into the dorsal spinal cord funiculus on the left side at T10-11 level to make located spinal cord injury models and randomly divided into four groups.The four groups were exposed to magnetic stimulation(1Hz,1.52T.30pulses)at the following dose respectively:Omg/kg U0126(control group).0.1mg/kg U0126(low-dose group), 0.2mg/kg U0126(middle-dose group),0.4mg/kg U0126(high-dose group).On the day 14 after stimulation,the rats were sacrificed and the expressions of glial fibfillary acidic protein(GFAP),microtubule associated protein-2(MAP-2),extracellular signal-regulated kinase1/2(ERg1/2)and the volume of holes were detected with immunohistochemistry.Quantitative analysis 0f the expressions of GFAP,MAP-2 and ERK1/2 were performed with the image analysis system.Result:With the increase of U0126 dose,the volume of hole increased on day 14(p<0.05).In the lesion area,the expressions of GFAP and ERK1/2 could be found,while MAP-2 could not.Significant differences were revealed in the expressions of GFAP、ERK1/2 among the four groups,it Was significantly lower in U0126 groups than that in control greup(P<0.05).while the middle-dose group had similar effect with the high-dose group(P>0.05).Conclusion:U0126 of different doses all could resupinate astrocyte migrations which were coused by low frequency magnetic stimulation,and 0.2mg/kg was the suitable dose.

Objective To investigate the effect of magnetic stimulation on astrocyte migration and its mech-anism. Methods Twenty-four adult, healthy Spragne-Dawley rats were injected with 0.5 μl of 1% ethidium bro-mide (EB) in the left side of the dorsal spinal cord funiculus at the T_(10-11) level to make a local spinal cord injury mod-el. They were then randomly divided into four groups and exposed to 30 pulses of magnetic stimulation at 1 Hz and the following intensities: O T (Group A);1.9x40% T (Group B); 1.9x80% T (Group C); 1.9x100% T (Group D). On the 14th day after stimulation, the rats were sacrificed and the expression of glial fibrillary acidic protein (GFAP), microtubule associated protein-2 (MAP-2) and extracellular signal-regulated kinase 1/2 (ERK 1/2) were detected, and the volume of holes in the injured area of the spinal cord was measured. Quantitative analysis of the GFAP, MAP-2 and ERK1/2 expression was performed using immunohistochemistry and an image anal-ysis system. Results The volume of holes in the injured area of the spinal cord decreased with increasing stimula tion intensity. In the reduced area of the holes, the expression of GFAP and ERK 1/2 could be seen, but not MAP-2. Significant differences were revealed in the expression of GFAP and ERK 1/2 among the four groups, but it was always significantly higher in the magnetic stimulation groups than in the controls. Conclusions After magnetic stimulation, astrocytes migrate into the injured spinal cord's holes. Astroeyte migration increases with increased mag-netic stimulation intensity, which may be associated with high expression of ERK 1/2.

Objective To assess the value of upper airway narrowing with MSCT under quiet respiration in patients with the obstructive sleep apnea-hypopnea syndrome (OSAHS). Methods CT scan of upper airway was performed in 63 patients with OSAHS during inspiration, expiration and quiet respiration respectively with 16-slice spiral CT. The state of the soft palate, the area of the smallest cross-sectional area of retropalatal and retroglossal space of the upper airway during the different respiratory phase were compared. The correlation between these dimensions and the polysomnography (PSG) results were analyzed. Results Some particular pattern of events occurred in 14 patients during the different phase of respiration, such as hooking of the soft palate, raising of the soft palate, artifacts, etc. These events occurred more often in the expiration phase. The cross-sectional area of the retropalatal space was the smallest during quiet breathing. There were statistically significant differences in the smallest cross-sectional area of retropalatal space among different phases of respiration (F=4.602, P＜0.05), also between inspiration and expiration, quiet breathing groups. No statistically significant difference was found between expiration and quiet breathing, nor in the smallest cross-sectional areas of retroglossal space among the three groups (P＞0.05). There was positive correlation between the smallest area of retropalatal space and LSaO_2 in quiet breathing and expiration groups (r=0.511, 0.300, P＜0.05). Conclusion MSCT scan of upper airway may be a better way to evaluate upper airway narrowing under quiet breathing when patients failed to carry out the required mode (keeping exhalation) or some undesirable events occurred.

It is important to establish a simple method of culture in vitro to obtain purity oligodendrocyte. This paper introduced the re-searches about co-culture in vitro with rat cortical gliocyte, proliferation and differentiation of oligodendrocyte progenitor, differentiation of oligodendrocyte and identification of cell surface antigens at different stages of development.

Purpose In order to find out a means that can both meet clinical requirements and reduce radiation dosage in the diagnosis of malignant biliary obstruction, the study aims to evaluate the image quality and the radiation dose of low tube voltage enhanced CT (100 kVp) in displaying malignant biliary obstruction in metal stents. Materials and Methods Between January 2012 and March 2014, 9 patients with highly suspected recurrent occlusion after biliary stent procedure were admitted in Peking Union Medical College Hospital and underwent high resolution three-phase dynamic enhanced CT scan. The CT images on portal veinal phase were reconstructed through the curved planar reformation and the CT values of 184 slices were evaluated along the pathway of the biliary stent. The reconstructed image quality was analyzed when the tube voltage was 140 kVp and 100 kVp respectively, and the different tumor-to-liver contrast-to-noise ratio (CNR) for each lesion, mean image noise, and figure of merit (FOM) between the two data sets were also compared. Results As the tube voltage decreased from 140 to 100 kVp, image noise increased from (19.88±3.62) HU to (24.22±4.24) HU (P<0.001), CNR increased by 15% and FOM improved by 50%. Conclusion A low tube voltage CT technique (100 kVp) improves tumor-to-liver CNR and significantly reduces radiation dose, therefore it is valuable in the manifestation of malignant obstruction in metal stents.

Acupuncture Points ; Acupuncture Therapy ; Cervical Vertebrae ; physiopathology ; Female ; Humans ; Middle Aged ; Treatment Outcome ; Vertigo ; physiopathology ; therapy