Objective To explore the value of chemosensitivity assay in vitro on breast cancer.MethodsIn vitro chemosensitivity of 6 species of chemotherapeutic agents applied to 38 cases of breast cancer patients were detected by tissue culture-end point staining-computer image analysis(TECIA).ResultsThe sensitivity to chemotherapeutic agents commonly used in the breast cancer level from high to low was as follow:Doxorubicin(ADM),Paclitaxel(TAX),Vinorelbine(NVB),Cyclophosphamide(CTX),Cisplatin(DDP)and Fluorouracil(FU).ConclusionDrugs sensitivity experiment of cancer in vitro by TECIA has an important value to instruct clinical medication and individual chemotherapy for breast cancer.

AIM: To establish the quality standard for Huangqi Fufang Granules(Radix Astragali, Fructus Schisandrae Chinensis, Radix Cyathulae, etc.). METHODS: Fructus Schisandrae Chinensis, Radix Cyathulae were identified by TLC, and the content of astragaloside Ⅳ was determined by TLC scanning. RESULTS: Fructus Schisandrae Chinensis, Radix Cyathulae could be identified by TLC. Astragaloside Ⅳ showed a good linear relationship at a range of 1.0?g～6.0?g, r =0.9992. The average recovery was 99.19%, and RSD was 0.63%. CONCLUSION: The methods are simple, accurate and specific, and can be used for the quality control of Huangqi Fufang Granules.