Objective: To study the method of extracting somatosensory evoked potential better. Methods: This article com-pares an auto-reference, auto-correlative and adaptive interference cancellation theories and techniques (AAA-ICT) used to the single trial of somatosensory evoked potential (SEP) with the traditional superposition averaging. Results: By the intensive study and analysis of the somatosensory evoked potentials from the 80 human subjects whose nervous systems are normal, We can find that the traditional superposition averaging method has its reasonable connotation on the extraction of SEP except the inevitable defects. Conclusions: Meanwhile the AAA-ICT avoids its defects. R implements another jump for the SEP extrac-tion method and has a good clinical application value.

To observe the effect of oxidized low density lipoprotein (OxLDL) on arterial endothelial cells apoptosis in vivo, we established a model in which Sprague-Dawley rats were given intraperitoneal and intravenous injection of unmodified LDL (8 mg/kg every day) via the tail vein. Seven days after the injection, the aortic endothelial cells specimens were prepared by an en face preparation of rat aorta. The apoptotic cells were identified and counted by in situ nick and labelling (TUNEL) method and light microscopy. The numbers of the apoptotic cells were 12.52 +/- 4.71/field in the intraperitoneal injection control group, 11.41 +/- 2.94/field in the intravenous injection control group, 22.98 +/- 8.01/field in the intraperitoneal injection LDL group and 103.8 +/- 11.5/field in the intravenous injection LDL group, respectively. The difference was significant between injection LDL group and control (P < 0.01), and the difference was also significant between two LDL injection groups (P < 0.01). These findings suggest that injection of LDL can induce apoptosis in arterial endothelial cells and the effect is especially significant with intravenous injection LDL. After injection, oxidative modification of LDL may occur in local arteries and causes injury to the endothelial cells.
Aorta ; Apoptosis/*drug effects ; Endothelium, Vascular/*pathology ; In Situ Nick-End Labeling ; Injections, Intraperitoneal ; Injections, Intravenous ; Lipoproteins, LDL/*metabolism ; Lipoproteins, LDL/*pharmacology ; Oxidation-Reduction ; Random Allocation ; Rats, Sprague-Dawley

Objective To investigate the effect of indirect gentle moxibustion on serum IL-1 and TNF-?contents in patients with knee osteoarthritis and explore the mechanism of its action. Method A randomized controlled trial was conducted according to the patient's condition. Besides acupuncture as basic treatment, the treatment group received indirect gentle moxibustion and the control group, thermotherapy plus cupping. Pre-treatment and post-treatment serum IL-1 and TNF-?contents and the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) scores were analyzed in the patients and compared between the two groups. Result There were statistically significant pre-/post-treatment differences in serum IL-1 and TNF-? contents and the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) score in the two groups (P<0.05). After treatment, serum IL-1 and TNF-?contents and the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) score were lower in the treatment group than in the control group. Conclusion Indirect gentle moxibustion can effectively decrease serum IL-1 and TNF-?contents in patients with knee osteoarthritis. That provides a certain molecular cytobiological and bio tissue engineering basis for the mechanism of its action in reducing inflammatory stimulation.

3626 cases of abdominal cystic mass wereclassified by authors. Results showed that ovarycystic mass was the most common among them.there were 2653 cases in all, accounting for73. 167%. In this paper 88 cases that were compar-atively rare were classified in details, according tooriginal tissue inflammatory cystic mass and reten-tive hydrops were the mose common, there were40 cases in all (45. 45%), congenital cystic masswas the second, 26 cases (29. 55%), developing lo-cation abserved was the most common in meson-tery, 32 cases (36. 36%), the second in abdominalcavity, and then, retroperitoneum, appendix, ete.

OBJECTIVE Through the cleaning effect of monitoring,understanding of the impact of mechanical cleaning and cleanliness factor.METHODS SonoCheck use of ultrasound to monitor the energy bottle,tcsi cleaning efficiency of the monitoring Browne STF cards and card detection cleansing effect on the ultrasonic energy and protein residue test equipment;set up observation ultrasonic cleaning time 5 to 25 minutes,cleaned 1 to 5 slot,and to observe the spray A cleansing poured procedures,according to the conventional function of the completion of cleansing,to observe the color change.RESULTS Energy in the ultrasonic testing only bottle in a clean yellow;washing machine to remove the cleaning efficiency of the red card to monitor pollution simulation,testing the effect of red cards pattern has not been completely clear.CONCLUSIONS Different cleaning supplies equipment for monitoring selective,while still concerned about the complexity of the cleaning equipment cleanliness,in order to maintain the supply of hospital centers and the quality of cleaning medical equipment unit.

The structural gene encoding mature peptide of extracellular ? amylase was amplified from the genome DNA of hyperthermophilic archaeon Pyrococcus furiosus by PCR The recombinant plasmid pUC19 amy was constructed by inserting the amplified segment into vector pUC19 The recombinant vector pYX212 amy was constructed by ligate the heterogeneous fragment of pUC19 amy into the multiple cloning site of pYX212, an expression vector of yeast Saccharomyces cerevisiae W303 A1 were transformed with pYX212 amy by electroporation The transformant expressed the activity of the thermophilic ? amylase successfully The recombinant enzyme has the similar enzymatic properties as the extracellular ? amylase produced by Pyrococcus furiosus : it shows an enzymatic activity optimum at pH 5 0, and its optimal temperature for enzymatic activity is about 90℃, more than 50% of its initial enzymatic activity is still detectable after it was incubated at 121℃ for 30 minutes

The knock out vector pHK was constructed with E coli vector pET 28a and shuttle vector pHY300PL, by using denatured DNA and homologous recombination technique, the kanamycin resistance gene ( Kan r) from integrated alkaline protease gene engineering strain BP071 was knocked out successfully, and the 11 positive clones were obtained The yield of the best positive clone BP0715 was stable as same as BP071 The methods provided the good experience for the industrial microbiology research, and it was foundation for studying on the safety of genetically modified organisms

The article introduce a rapid method for preparation of fungal chromosome DNA In this method the quartz sand is used to break the fungal cell wall and the chromosome DNA is harvested rapidly in 1～2 h The method is applied successfully by the author to four kinds of fungi Neurospora crassa , Aspergillus oryzae , Morchella esculcnta , Saccharomyces cervisae All the chromosome DNA extracted has the fragment size lager than 20 kb and can be used directly for either digestion with restriction endoenzyme or PCR

OBJECTIVE To explore an effective cleaning method to improve the washing quality of contaminated complicated medical instruments.METHODS The used puncture needles were washed by three methods(ultrasonic wave only,ultrasonic wave + polyenzyme detergent,ultrasonic wave + spray washing with special washing-rack designed by ourselves,while artificial washing group as control group,3 pieces per group).After washing,the samples were further assayed with HemoCheck-s swab to compare the washing effect.RESULTS The color of cotton swabs was turned blue or light blue.CONCLUSIONS Our results have demonstrated that ultrasonic cleaning plus spray washing with special washing-rack can improve the cleaning quality.

Based on the application of the second generation field medical cabin in the practices,the author points out the advantages of it such as advanced equipment,diversified adaptation,fully equipped element,etc.Meanwhile,the author also puts forward problems and relevant suggestions in extension area,equipment configuration and accessories.The suggestion provides basic theoretical evidence for improving the medical cabin devices.