Marine medicine is an important part of Chinese medicine and a growth point of the development of marine economy. Current researches of marine medicine had ignored accumulated experience in clinical medicine for thousands of years of development of marine medicine and the guidance of traditional Chinese medicine (TCM) theory. In this article, we proposed a research mode of Mining experience-Clinical validation-Targets screening-Component screening-Component compatibility-Mechanism study under the guidance of the hypothesis of Nature-Effect-Material Trialism to study marine medicine. Kun Hai Jiang Xin (KHJX) Decoction is a marine formula drawn from the experience of ancient physicians by data mining techniques. Clinical study found that KHJX Decoction can significantly improve the overall clinical efficacy of chronic bronchitis with good clinical safety. Under the guidance of TCM herbal theory, object presented here is based on the work of KHJX Decoctionmining experience - clinical validation. The molecular docking method and receptor-ligand binding assay were applied to screen effective components from herbs of KHJX Decoction and the compatibility plan of effective components under the guidance of the principle of cold and heat effects compatibility. This study is of great significance to clarify the material basis and molecular mechanism of KHJX Decoction in the treatment of chronic bronchitis. It provides a new research mode for marine drug research.

Although the origin of Chinese medicine was well recorded inHuang-Di Nei-Jing, different opinions, however, now existed in the authenticity of discussions on five cardinal directions in theYi-Fa Fang-YiLun. From the perspective of regional systems, this paper began with the local culture of Chinese northern nomadic people, analyzed their fire using custom, moxibustion therapy, ice and snow culture and Shamanistic witchcraft. The purpose was to reveal that moxibustion therapy originated from Shamanistic witchcraft and medical practice of the northern nomadic people.

Calcium content and calcium-calmodulin complex were determined in endothelial cells before and after they were treated with endotoxin.It was found that endotoxin can promote the disintegration of calcium-calmodulin complex and greatly increase the cacium content in the endothelial cells.

0.05) in results. The positive rate was 26.99% for these 3 kits, and any two of them could achieve a positive rate of 53.95%. The total positive rate was 80.95%, which was significantly different as compared with non-tuberculosis group(P

Objective To investigate the dynamic changes of Trk(tropomyosin receptor kinase) and Akt(protein kinase B), and to explore the neuroprotective role and its mechanism of β-NGF (β-nerve growth factor) after focal cerebral ischemia. Methods A focal cerebral ischemia rat model was made and β-NGF was injected intra abdominally. The phosphorylation of Trk and Akt were tested by immunocytochemistry and Western blot assay. Results Trk receptor was increased obviously in the penumbra area after 8 h of infarction. The level of phosphorylated Trk(p-Trk) was increased after 2 h of infarction, while the level of phosphorylated Akt(p-Akt) had a decrease and then recovered gradually. The level of p Akt in cerebral ischemia group was decreased by 76.5% compared with that in control group after 8 h of infarction(P<0. 01). After the injection of β-NGF, the level of p-Trk was increased by 74.4% after 12 h of infarction(P<0.01), while the level of p-Akt was recovered significantly after 8 h of infarction, and had no statistical difference compared with the control group after 24 h of infarction (P>0.05). Conclusions Ischemia induces the activation and increased expression of Trk receptor, andβ-NGF may play a protective role in cerebral ischemia by increasing the phosphorylation of Trk and regulating the phosphorylation of Akt.

Twenty-nine medical records of acupuncture and moxibustion at the end of volume in Zhenjiu Dacheng are precious clinical examples. The characteristics of selecting acupoints are less and ingenious with precious matching on the basis of syndrome differentiation and examining pathogenesis detailedly, according to meridians with diseases locations recognized clearly, keeping adopting acupoints on regular meridians combined with extra points and making good use of specific points, and attaching importance to acquired essence with protecting spleen and stomach. Valuable experience is provided for physicians of later generations to treat diseases.
Acupuncture Points ; Acupuncture Therapy ; history ; Books ; history ; China ; History, Medieval ; Humans

Objective To observe the prognosis 90 days after attack and the correlation among various risk factors in patients with transient ischemic attack (TIA). Methods The data of 90 days follow up and related risk factors of 61 patients clinically diagnosed with TIA were analyzed.We also compared the prognosis of those patients according to the change on diffusion weighted imaging (DWI). Results The incidence of various kinds of severe vascular events in 61 patients within 90 days was 16.4% (10/61). Among the patients with abnormal change on DWI, the incidence was 50% (8/16). However the incidence was 4.7% (2/43) in the patients with normal DWI. There was a statistical difference between the two groups (P

Objective To comparative the expression of suppressors of cytokine signaling 3 (SOCS3) in placental tissues between premature delivery and term delivery,and to evaluate the significance of SOCS3 in premature-delivered placental tissues.Meth ods Retrospective analysis was made on 30 patients who had spontaneous premature delivery and 30 pregnant women with normal delivery from February 2013 to October 2013 in our hospital.Reverse transcription and polymerase chain reaction (RT-PCR) was used to detect the mRNA expressions of SOCS3,interferon-α(IFN-α),and interleukin-10 (IL-10).Western-blot was used to detect protein expression of SOCS3,and to analyze the balance of Th1/Th2.Results PCR detection showed that SOCS3-mRNA in the control group was significantly lower than that in the experimental group (P ＜ 0.01).Western-blot test showed that SOCS3 protein expression in the control group was significantly lower than that in the experimental group (P ＜ 0.01).The expression of SOCS3 gene was consistent with its protein expression.PCR detected the significant increase of IFN-α with the secretion of Th1 cytokine,significant decrease of IL-10 with secretion of Th2 cytokine (P ＜ 0.05),and the balance deviation of Th1/Th2 toward Th1.Conclusions SOCS3 is associated with premature delivery,which mechanism might be involved in regulation of the immune balance through janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathways.

A generator has been designed to generate simulating underpressure of blast wave and applied for the study of underpressure injuries on animals.The generator is composed of a vacuum pump,a hypotcnsive chamber,an experimental chamber,a membrane-rupturing device and a pressure-measuring system.Its operating principle is as follows:When the pressure of the hypotensive chamber is being decreased by the drawing of the vacuum pump to a given level .the membrane separating the hypotensive chamber and the experimental chamber is rapidly perforated by a sharp pointer.Underpressure occurs in the experimental chamber where the animal is fixed as soon as the pressure changes instantaneously.The range of the physical parameters of the simulated underpressure are -13 to -90 kPa for the peak level,1 ms to 90 ms for the decreasing time,and 14 ms to 2000 ms for the duration.Animal experimentation revealed that this device is capable of inflicting injuries and verified the possibility that the underpressure of blast waves can result in injuries.

Objective To evaluate whether P53 gene mutation was related with the pathogenesis of ovarian malignant epithelial tumors or not.Methods P53 gene mutation in exons 5-8 was detected by polymerase chain reaction product-single strand conformation polymorphism analysis (PCR-SSCP).Results The mutation in benign tumors: 3(10%) cases, but in malignant epithelial tumor:19(38%) cases(P0 05).Conclusions P53 gene mutation in exons 5-8 was probably related with the pathogenesis of ovarian malignant epithelial tumors, but was not related with histological grade.