Objective To investigate the effect of hand twelve Jing-Well points bloodletting on blood-brain barrier (BBB) permeability in the early stage of focal cerebral ischemic injury in rats with permanent middle cerebral artery occlusion (pMCAO). Method Male Wister rats were randomized into sham operation, model and bloodletting groups. A model of middle cerebral artery occlusion was made by Longa's method. 2%Evans blue (EB) was injected into the caudal vein at three time points:5, 24 and 72 hrs after model making. EB exudation amount was assessed by measurement of EB absorbance in brain tissue to observe BBB permeability. Result Cerebral EB exudation amount was significantly higher in the model group of rats than in the sham operation group (P<0.01) and significantly lower in the bloodletting group than in the model group (P<0.01). Conclusion Hand twelve Jing-Well points bloodletting can reduce blood-brain barrier permeability in pMCAO rats. Its effect is highly significant at 24 and 72 hrs after ischemia.

Objective To study the relationship between obstructive sleep apnea hypopnea syndrome(OSAHS)and carotid atherosclerosis in ischemic stroke patients.Methods 43 subjects with ischemic stroke were enrolled and divided into carotid atherosclerosis group and carotid normal group according to the intima-media thickness(IMT)and plaque of carotid arteries,which determined with high-resolution ultrasound.Polysomnography(PSG)was performed for all the patients and record the apnea-hypopnea index(AHI),oxygen desaturation index,deep duration with SaO2

Objective To observe the subarachnoid space the epidural space joint block labor analgesia (CSEA) changes of T lymphocyte subsets.Methods 100 cases of full-term mothers were randomly divided into two groups,analgesia group and the control group.The analgesic group row CSEA childbirth analgesia.Two groups of maternal cervix large 2 to 3cm (T1),when the baby was delivered (T2),24h (T3),blood was colleoted after childbirth,detected by radioimmunoassay of cortisol.Flow cytometry assay was wsed to test T lymphocyte subsets CD3+,CD4+,CD8+,CD4+/CD8+,And the birth process,Apgar score,Labor pain visual analogue score (VAS) were recoroded.Results (1) Active phase of the first stage of childbirth analgesia group was significantly shorter than the control group (t =2.182,P ＜ 0.05).The second and third stage of Childbirth and neonatal Apgar score difference between the two groups was not statistically significant.(2)Serum cortisol concentration at T2 analgesia group was significantly lower that of than the control group (P ＜ 0.05).(3) The two groups in maternal blood of CD3+,CD4+,CD4+ / CD8+ were reduced to varying degrees,T3 was reduced significantly (P ＜ 0.05),Analgesia group was significantly lower than that of the control group (all P ＜ 0.05).Conclusion CSEA analgesia in Childbirth can shorten the first stage of Childbirth active period,has no significant effect on the Apgar score,can alleviate the pain and stress reactions such as the suppression of the maternal immune function.

Objective To observe the effect on body distribution tendency of paclitaxel liposome in the lung cancer rats after acupuncture of Feishu,Taiyuan acupoints in order to develop the new path for the target study of anticancer drugs.Method The paclitaxel liposome was labeled by technetium(Tc).Four methods including tail intravenous injection of paclitaxel liposome merely and the combination of tail intravenous injection of paclitaxel liposome with acupuncture of Feishu,Taiyuan(concerned acupoint) and Shenmen(unconcerned acupoint) acupoints respectively were carried on the intervention to the lung cancer rat models(18 in each group).The rats were sentenced to death at 60,90,120 minutes(6 at each time point) respectively after drug injection,and the drug content of paclitaxel liposome in the lung,liver,kidney organs were measureed by the ?-radiate immunity counting implement respectively.Result Acupuncturing at Feishu and Taiyuan can lead to the drug content increment of paclitaxel liposome in the lung organization of the lung cancer rats in all or parts of time points.On the contrary,acupuncturing at Shenmen can not.Conclusion Acupuncturing Feishu and Taiyuan can make tropism effect on the disposition of paclitaxel liposome in vivo in lung cancer rats.Feishu is stronger than Taiyuan on the drug tropism of the lung.

Objective To investigate the calcium mechanism involved in the A? 25-35-induced neurotoxicity.Methods PC12 cells viability was detected by using MTT assay. Relative change of intracellular calcium concentration was measured by laser confocal microscope.Results MTT assay showed that the cell viability of the three groups (10 ?mol/L A? 25-35?10 ?mol/L A? 25-35+5 ?mol/L nifedipine?10 ?mol/L A? 25-35+10 ?mol/L nifedipine) was decreased by 34.5%, 25.1% and 11.0%,as compared with the control group. 10 ?mol/L nifedipine could protect PC12 cells from A? 25-35-induced damage. A? 25-35 of different concentration (0.1 ?mol/L, 1 ?mol/L, 10 ?mol/L, 20 ?mol/L and 30 ?mol/L) resulted in the elevation of intracellular calcium (about 6.38%, 6.42%, 62.2%, 69.3% and 107.5%) with a dose-dependent manner. Potassium (5 mmol/L, 15 mmol/L and 30 mmol/L) could increase the intracellular calcium after one minute pretreatment of different concentration A? 25-35. Both above mentioned actions were sensitive to the medium calcium and were antagonized by nifedipine, a L-voltage-gated calcium channel antagonist.Conclusion Soluble A? 25-35 may damage the neuronal calcium homeostasis in early stage and make neurons more vulnerable to physiological or pathological stimuli.

Twenty-nine medical records of acupuncture and moxibustion at the end of volume in Zhenjiu Dacheng are precious clinical examples. The characteristics of selecting acupoints are less and ingenious with precious matching on the basis of syndrome differentiation and examining pathogenesis detailedly, according to meridians with diseases locations recognized clearly, keeping adopting acupoints on regular meridians combined with extra points and making good use of specific points, and attaching importance to acquired essence with protecting spleen and stomach. Valuable experience is provided for physicians of later generations to treat diseases.
Acupuncture Points ; Acupuncture Therapy ; history ; Books ; history ; China ; History, Medieval ; Humans

Objective To establish normal reference interval for four items of blood coagulation on ACL‐TOP Automatic coagu‐lation analyzer .Methods The fasting anti‐coagulation blood samples were collected from 1 268 inpatients and people conducted physical examination ,all subjects without liver disease ,history of blood disease and coagulation disfunction .The prothrombin time (PT) ,activated partical prothrombin time(APTT) ,thrombin time(TT) and serum levels of fibrinogen(FIB) were determined by u‐sing ACL‐TOP automatic coagulation analyzer which was producted by America IL company .And data of determination results were used to establish the normal reference intervals of indexes in this laboratory .Results The precision and accuracy of this analy‐zer was good .There were differences of normal reference intervals between which established in this laboratory and which provided by the manufacturer .Conclusion Each laboratory should establish its own normal reference interval ,not blindly refer to reference interval provided by regents manual .

Objective To investigate the clinical effects of Compound Biejia Ruangan Tablet (CBRT) combined with entecavir in patients with chronic hepatitis B with hepatic fibrosis.Methods Totally 92 cases of patients with hepatic fibrosis of chronic hepatitis B in Ankang people's hospital from January 2013 to May 2016 were divided into observation group (n =45) and control group (n =47),patients in observation group were treated with CBRT combined with entecavir,and patients in the control group were treated with entecavir.Treatment was for 48 weeks.The liver function,liver fibrosis,serum inflammatory factors,and adverse reactions were compared between two groups.Results After treatment,the ALT,AST,TBIL,and ALB/GLB levels of two group were significantly lower than those before treatment (P ＜ 0.05),but there was no significant difference between two groups after treatment.After treatment,the PCⅢ,IV-C,HA,LN,IL-6,IL-4,IL-10,and TNF-α levels of two group were significantly lower than those before treatment (P ＜ 0.05),and the PCⅢ,IV-C,HA,LN,IL-6,IL-4,IL-10,and TNF-α levels of observation group were significantly lower than those of control group (P ＜ 0.05);there was no statistically significant difference in the incidence of adverse reactions between two groups (P ＜ 0.05).Conclusion CBRT combined with entecavir is safe and effective to treat liver fibrosis of chronic hepatitis b,and it could significantly improve liver function,improve the degree of liver fibrosis,and reduce the level of serum inflammatory factors.

Objective To evaluate the efficacy of preoperative and postoperative intravesical instillation of pirarubicin for the prevention of postoperative recurrence of non-muscle invasive bladder cancer (NMIBC). Methods From September 2012 to March 2014, 120 patients who were diagnosed as NMIBC and accepted trans-urethral resection of bladder tumor (TUR-Bt) were collected and randomly divided into two groups, including treatment group (60 cases) and control group (60 cases). 30 minutes before the operation, instillation of pirarubicin (40 mg) was performed in the treatment group, then pirarubicin perfusion was given within 24 h after operation, once a week for 8 weeks, and once a month for 10 months after operation. In control group, instillation of pirarubicin (40 mg) was given within 24 h after operation, and other treatments were the same as the treatment group. The differences of lesion detection probability, the 2-year recurrent rate and the adverse reaction rate were compared between the two groups. Results The lesion detection rates were 42.70 % (38/89) and 1.85 % (2/108) in the treatment group and control group, respectively (χ2=50.303, P<0.05). 119 patients were follow-up for 24 months. The 2-year recurrence rates were 8.47%(5/59), 21.67% (13/60) in treatment group and control group respectively (χ2= 4.033, P< 0.05). The adverse reactions mainly included hematuria (3 cases in treatment group, 3 cases in control group), urinary tract irritation (3 cases in treatment group, 2 cases in control group) and urethral stricture (1 case in treatment group, 1 case in control group). The difference of the adverse reaction incidence between the two groups was not significant statistically [11.86 % (7/59) vs. 10.00 (6/60), χ 2 = 0.106, P > 0.05]. Conclusions The preoperational intravesical instillation of pirarubicin can fix and diagnose malignant lesions in the bladder without adverse reactions, which will improve the rate of tumor resection and prevent postoperative recurrence of tumors.

Objective To pool the data of studies about bosentan therapy in pulmonary hypertension (PAH) and evaluate the efficacy and safety of bosentan therapy. Methods A search was performed in PubMed (from 01/01/1968 to 01/05/2006), Embase (from 01/01/1980 to 01/05/2006) and China National Knowledge Infrastructure (CNKI) (from 01/ 01/ 1978 to 01/ 05/ 2006) to identify relevant articles. Randomized-Controlled Trials (RCT) about the efficacy and safety of bosentan therapy in PAH patients were included to evaluate the short-term and long-term efficacy and safety of bosentan therapy in a method of Meta-analysis. Results (1)Two RCT studies including 243 PAH patients were chosen to evaluate the short-term efficacy and safety of bosentan therapy. Compared with placebo treatment, bosentan therapy could improve exercise capacity of PAH patients:① Distance covered in a 6-minute walk test increased 47.71 meters (95% CI:26.09 to 69.33, P