[Objective]To explore the effects of Chinese traditional medicine and bone marrow transplantation(BMT) on delayed union.[Method] The model of delayed union in rabbit's radius was made and divided into 4 gronps.Group A,20 rabbits treated with Chinese traditional medicine and red marrow 2 ml;Group B,20 rabbits treated with Chinese traditional medicine;Group C,20 rabbits treated with red marrow;Group D,15 rabbits treated with nothing.Histology,alkaline phosphates and xray were observed in the 4th,8th and 12th weeks after treating.[Result] Histology,alkaline phosphates and x-ray reveals that group A healed earlier than group B,C and D.At the same time,the rate of healing of group A was higher than others.[Conclusion] The treatment of delayed union by Chinese traditional medicine and bone marrow transplantation is an effective mean.

OBJECTIVETo determine whether lysosome-associated protein transmembrane-4 beta (LAPTM4B) over-expression is associated with the proliferation and invasion in colorectal cancer (CRC).

METHODSThirty pairs of CRC tissues, containing carcinoma and adjacent tissues, were used for the examination of LAPTM4B mRNA expression by real-time quantitative PCR (qPCR) assays. Then immunohistochemistry was performed to examine LAPTM4B protein expression in 6 pairs of CRC tissues. Over-expression LAPTM4B and low-expression LAPTM4B cell models were constructed with HCT116 CRC cell lines. CCK8 assay was used to detect the proliferation and Transwell assay was used to detect the invasion of the model cells.

RESULTSqPCR and immunohistochemistry results showed that LAPTM4B expression levels in CRC were higher compared to adjacent tissues (all P<0.01). CCK8 and Transwell assays results showed that LAPTM4B promoted proliferation and invasion of HCT116 cell lines model cells (all P<0.01).

CONCLUSIONLAPTM4B promotes the proliferation and invasion in CRC patients, and may be used as an important potential marker.

Cell Proliferation ; Colorectal Neoplasms ; HCT116 Cells ; Humans ; Immunohistochemistry ; Membrane Proteins ; Neoplasm Invasiveness ; Oncogene Proteins

Objective To determine whether lysosome-associated protein transmembrane-4 beta (LAPTM4B) over-expression is associated with the proliferation and invasion in colorectal cancer (CRC). Methods Thirty pairs of CRC tissues, containing carcinoma and adjacent tissues, were used for the examination of LAPTM4B mRNA expression by real-time quantitative PCR (qPCR) assays. Then immunohistochemistry was performed to examine LAPTM4B protein expression in 6 pairs of CRC tissues. Over-expression LAPTM4B and low-expression LAPTM4B cell models were constructed with HCT116 CRC cell lines. CCK8 assay was used to detect the proliferation and Transwell assay was used to detect the invasion of the model cells. Results qPCR and immunohistochemistry results showed that LAPTM4B expression levels in CRC were higher compared to adjacent tissues (all P<0.01). CCK8 and Transwell assays results showed that LAPTM4B promoted proliferation and invasion of HCT116 cell lines model cells (all P<0.01). Conclusion LAPTM4B promotes the proliferation and invasion in CRC patients, and may be used as an important potential marker.

Objective:To summarize the experience in surgical treatment for ulcerative colitis(UC).Methods:The clinical data of 36 patients with UC who underwent surgery in Renji Hospital,Shanghai Jiao Tong University School of Medicine from Jan 2008 to Jan 2013 were retrospectively analyzed.The surgical methods and prognosis were evaluated.Results:There were 23 ca-ses of selective operation which was total colectomy with ileal pouch-anal anastomosis(IPAA)in the first stage.Among the 23 cases,4 cases complicated with toxic megacolon were treated with intestinal obstruction catheter by endoscopy,and then under-went selective IPAA.There were 13 cases of emergency operation which was total colectomy with ileostomy in the first stage and IPAA in the second stage.Open surgery was done in 1 patient with perforation of digestive tract,1 patient with toxic mega-colon and 1 patient with history of abdominal operation,and laparoscopic surgery was done in the other 33 patients.None of the 36 cases had any severe perioperative complications.Conclusions:Indication of UC operation should be strictly controlled while safe operation method should be reasonably chosen.IPAA is a safe and effective method for selective operation and emergency surgery of UC.

Objective To determine whether lysosome-associated protein transmembrane-4 beta (LAPTM4B) over-expression is associated with the proliferation and invasion in colorectal cancer (CRC). Methods Thirty pairs of CRC tissues, containing carcinoma and adjacent tissues, were used for the examination of LAPTM4B mRNA expression by real-time quantitative PCR (qPCR) assays. Then immunohistochemistry was performed to examine LAPTM4B protein expression in 6 pairs of CRC tissues. Over-expression LAPTM4B and low-expression LAPTM4B cell models were constructed with HCT116 CRC cell lines. CCK8 assay was used to detect the proliferation and Transwell assay was used to detect the invasion of the model cells. Results qPCR and immunohistochemistry results showed that LAPTM4B expression levels in CRC were higher compared to adjacent tissues (all P<0.01). CCK8 and Transwell assays results showed that LAPTM4B promoted proliferation and invasion of HCT116 cell lines model cells (all P<0.01). Conclusion LAPTM4B promotes the proliferation and invasion in CRC patients, and may be used as an important potential marker.