Cardiovascular disease caused by atherosclerosis has been the world′s primary cause of death. Studies have shown that early intervention in preclinical stage of atherosclerosis among children can reverse the disease and ultimately reduce the morbidity of atherosclerosis related diseases after they grow up,which is crucial to reduce and eliminate the harm of atherosclerosis.Now,the risk factors and detection methods of children′s preclinical atherosclero-sis were reviewed.

Objective To discuss the effects of peritoneal dialysate (PDS) on the expression of aquaporin-1 in rat peritoneal mesothelial cells (RPMCs). Methods The in vitro cultured RPMCs were divided randomly into 4 groups, and stimulated respectively with 1640 culture medium containing 10% fetal calf serum (negative control), and three kinds of PDS (containing respectively 4.25% glucose, 4.25% mannitol or 1.5% glucose) for 3 hours. Indirect immunofluorescence assay (IFA) and flow cytometry (FCM)were employed to detect the expression of aquaporin-1 in RPMCs stimulated by different kind of PDS as above. Results Compared with negative control and the group with PDS containing 1.5% glucose, the expression of aquaporin-1 on the mesothelial cells of the group with PDS containing 4.25% glucose and that containing 4.25% mannitol was significantly up-regulated (P0.05). Conclusion PDS with higher osmotic pressure (OP) can enhance the expression of aquaporin-1 in RPMCs. For PDS with same OP (PDS containing 4.25% glucose or PDS containing 4.25% mannitol), those containing glucose can enhance the expression of aquaporin-1 more effectively than that PDS containing mannitol. This result indicates that glucose or its degradation products may be the independent factors in enhancing the expression of aquaporin-1 of RPMCs.

OBJECTIVE To evaluate the in vitro inhibitory effects of antibacterials in combination carbapenem against multiresistant Pseudomonas aeruginosa.METHODS The protocol was designed by checkboard method and the MIC of the two antibiotics in combination was determined by broth dilution method.FIC was calculated according to MIC results.RESULTS The MIC50 and MIC90 of eight kinds of antibiotics were lower to 1-16 times and 1-64 times,respectively.The synergistic and additive antibacterial activity was seen in ceftazidime with amikacin,piperacillin with tobramycin and azlocillin with sulbactam.The lomefloxacine with ampicillin and lomefloxacine with ceftriaxone were not correlated.CONCLUSIONS Antibacterial activity of ceftazidime combined with amikacin against carbapenem-multiresistant P.aeruginosa is the best.

Objective To investigate the effects of laparoscopic tube sparing operation in the treatment of tubal ectopic pregnancy. Methods Clinical data of 56 cases of tubal pregnancy undergoing laparoscopic conservative treatment were retrospectively reviewed. Results Salpingectomy was required in 8 cases because the electrocoagulation had proven ineffective for the bleeding of operated area, whereas the tube sparing surgery was accomplished successfully in the remaining 48 cases, in 3 of which one treatment course of MTX was administered on account of recurrent episodes. Hysterosalpingography with iodized oil at 1～2 postoperative months in 47 cases receiving conservative surgical treatment revealed that, the involved oviduct was patent in 36 cases, unobstructed but not patent in 8 cases, and obstructed in 3 cases, while the contralateral oviduct (which had been previously resected in 3 cases) was patent in 34 cases, unobstructed but not patent in 7, and obstructed in 3. Conclusions Under proper conditions, laparoscopic tube sparing operation is effective in the treatment of tubal ectopic pregnancy.

Objective To investigate the effect of vitamin E supplementation on oxidative stress in hemodialysis patients. Methods Fifty-six uremic patients undergoing hemodialysis (HD) were involved in this self-controlled study. The patients were observed for one month to evaluate the status of oxidative stress and then divided into two groups randomly. Group A patients (n=28) received 200mg/d of vitamin E and Group B patients (n=28) were treated with 400mg/d of vitamin E. Vitamin E was supplied for one month in both groups. The serum levels of advanced oxidation protein products (AOPP), malondialdehyde (MDA), vitamin E and activity of glutathione peroxidase (GSHPx) were measured at the initial and the end of vitamin E supplementation respectively. Results Serum levels of AOPP and MDA were significantly higher in HD patients compared with healthy controls (n=56, P

Objective To elucidate the mechanism of accelerated apoptosis of monocytes in patients with lupus nephritis (LN). Methods 17 LN patients were enrolled in the study and 15 healthy volunteers were selected as controls. The expression of Faa and Fas ligand (FasL) was examined by immunofluorescent staining and flow cytometer analysis. The apoptosis and survival rate of monocytes cultured in vitro with human FasL (rHu-FasL) was analyzed by flow cytometer analysis and 5-diphenyl tetrazolium bromide (MTT) staining, respectively. Results Faa expression on monocytes was significantly higher in LN patients than that in healthy subjects. There was no difference of monocytes Fas expression between active LN patients and silent LN patients. There was no detectable FasL expression on monocytes from both LN patients and healthy controls. When monocytes cultured in vitro with rHu-FasL, there was a significantly increasing apoptosis and a decreasing survival rate of monocytes in LN patients as compared to healthy controls. Conclusion The up-regulated expression of functional Fas on monocytes may contribute to the accelerated apoptosis of monocytes in LN patients.

Objective To compare the Cosine and Dip methods in describing the rhythm of blood pressure(BP).Methods The BP was measured with non-traumatic ambulatory blood pressure monitoring(ABPM) for 24 h and analyzed with Cosine and Dip methods.The characters of the circadian rhythm of BP were studied and two methods were compared.Results The ratios of mesor and amplitude(A/M) of systolic blood pressure(SBP) and diastolic blood pressure(DBP) were(7.78?0.42)% and(7.98?0.39)%,respectively.The majority of acrophases of BP was on daytime,especially between 10∶00 am and 3∶00 pm.The amplitudes of acrophase in different periods analyzed with Cosine method were not significantly different each other,whereas analyzed with Dip method to be significant difference.Conclusion There is difference between Cosine and Dip methods in describing the rhythm of BP,since rhythms of acrophases of some cases were inaccurately described with Dip method.

ObjectiveTo evaluate arterial stiffness and its influencing factors in the elderly patients with hypertension and (or) type 2 diabetes mellitus.MethodsTotally 320 elderly patients were divided into four groups: control,diabetes mellitus,hypertension,coexisting hypertension and diabetes mellitus.Arterial stiffness in ankle and brachial artery was determined by brachial-ankle pulse wave velocity (baPWV).Other factors affecting baPWV were also collected and measured,including age,body mass index (BMI),sex,smoking,blood pressure,pulse pressure,mean arterial pressure,serum fasting blood glucose,lipid profile,glycosylated hemoglobin and hypersensitive C-reactive protein (hsCRP).ResultsbaPWV in the four groups were ( 1667.2 ± 279.3 )cm/s,(2158.6 ±386.9)cm/s,(1881.2±383.8) cm/s and (2165.9± 479.9)cm/s,respectively.The baPWV value was higher in groups of hypertension,diabetes mellitus,and the coexisting diseases than in controls (F=8.473,P＜0.05).Diabctic patients had a higher baPWV levels than those without diabetes.There was no difference in baPWV levels between diabetic patients with or without hypertension.Pulse pressure and hsCRP were higher in coexisting diseases group than in control group (P＜0.05).Stepwise backward regression analysis showed that fasting blood glucose,pulse pressure,mean blood pressure,hsCRP and low density lipoprotein cholesterin (LDL-C) were positively related to baPWV P ＜0.05). ConclusionsHypertension and (or) type 2 diabetes mellitus are important influencing factors for higher arterial stiffness. Hyperglycemia might play more important role in the elderly arterial stiffness.Serum fasting blood glucose,pulse pressure,mean blood pressure,hsCRP,LDL-C may be the independent risk factors of arterial stiffness.

Objective To detect the expression of DBC2 and the methylation status of its gene promoter region in gastric cancer,so as to analyze their correlation. Methods Gastric cancer tissues and adjacent normal tissues were collected from 92 cases as research subjects. The DBC2 mRNA expression and the methylation status of DBC2 gene promoter region in gastric cancer were detected by using RT?PCR and MS?PCR respectively. In addition,the correlation of DBC2 gene methylation status with clinicopathological parameters was analyzed. Results In comparison with adjacent normal tissues,gastric cancer tissues displayed a low expression level of DBC2mRNA and a high methylation level of gene promoter region. Besides, the methylation level was significantly correlated with tumor invasion depth,differentiation and TNM staging(P<0.05). With deep invasion,poor differentiation or higher TNM staging,methylation level of gene promoter region was more significant in gastric cancer tissues. Conclusion High methylation of DBC2 gene promoter region may be a significant contributing factor leading to low DBC2 expression.