In this paper,we reported that T cell,T cell subpopulation and natural killer(NK)cell activities of peripheral blood lymophocytes from the Fatients with tumor weredetected with OKT monoclonal antibody and ~(51)Cr release cytotoxity test.The resultsshowed that percentage of OKT3~+,OKT4~+ cell in the peripheral blood of patients withtumor are low,OKT8~+ is high and the ratio of OKT4/OKT8 is low.The results haveproved that the ratio of OKT4/OKT8 and NK cell activities were significantly low inthe peripheral blood of patients with tumor.However,the activities have restored tonormal or near normal level in the patients after surgical operation for more that twoyears with no recurrence. Our data suggest that detecting of the ratio of OKT4/OKT8 and activities of NKcell might be valuable to the prognosis of patients with tumor.

Objective To analyze the reasons of the common complications happened after the operation of congenital syndactyly and to find the method of prophylaxis and treatment,and to improve the effects of treatment.Methods To classify the reasons of the complications happened after the operation of congenital syndactyly in 34 cases.Results The reasons of the complications included that the patients were not operated by special course of study doctors in 9; operation at an inappropriate opportunity in 8;the method of web taken shape was improper in 7;the method of nail taken shape was improper in 4; the fixation and training was unsuitable in 6. Conclusions The complications happened after the operation of congenital syndactyly will be reduced much , provided that grasping the right opportunity and selecting the proper method of web or finger tip taken shape before operation , taking care of the transformations of blood vessel and nerve in operation , fixing firmly and training appropriately after operation.

Objective To introduce a method of distal digital replantation with a venous drainage through medullary cavity.Methods Normal saline mixed with methylene b lue were perfused into digital arteries in 5fresh finger specimens and medullary cavities in 10fresh finger specimens.Then microanatomic al observation of vessels was performe d in the specimens.From April 1999to June 2000,15severed fingers in14cases were replanted with a venous drainage through medullary cavity.Medullary cavities of the distal and middle phalanx were drilled and made unobstructed after the distal joint of the finger was removed.The bones were fixed using only a single straig ht Kirschner wire in each finger.One or two digital arteries or the branch es of the terminal arteries were anasto mosed and two digital nerves sutured in each finger.Results Mi-croanatomical observation showed t hat a venous drainage through medullary cavity was dominating without su-perficial venous drainage and the di gital medullary cavity closely connected with the profunda digital vein s and the superficial digital veins by the venous system of the bone.All replan ted fingers survived in 14cases.The average postoperative follow-up wa s 7.8months,ranging from 6to 10mont hs.Nearly normal appearance and satisfactory sensation of the dista l phalanx were obtained with 2-PD of 5-9mm.Conclusion Distal digital replantation with a venous d rainage through medullary cavity is a new method.It has following advan-tages:simple and reliable operatio n,high survival rate and enlarged in dication of distal digital replanta tion.[

Objective:To study comparitively the iIEL activation of somatic polysaccharose of bifidobacteria and E.coli.Methods:Intestinal intraepithelial lymphocyte(iIEL)is isolated from small intestine using Percoll discontinuous density gradient centrifugation At the same time, somatic polysaccharose of bifidobacteria and E.coli are isolated by organic solvent and are cultured with iIEL,then the natural killing activity of iIEL and ability to produce interleukin 2(IL 2)were examined Results:Somatic polysaccharose of bifidobacteria and E.coli significantly enhance natural killing activity of iIEL and its ability to produce IFN? and IL 2 (P

Objective:To extract DNA from Bifidobacteria and E.coli cells and incubate DNA with IEL to observe.DNA's activation for IEL.Also plan to use the DNA to immune mice to observe the change of immunologic function,discuss the effect of Bifidobacteria and E.coli's whole DNA to murine and make a comparision study.Methods: Mice of Bifidobacteria DNA and E.coli's DNA treated groups are respectively given with DNA by muscular and subcutaneous injection. Detect the immunologic function of spleen cell, extract IEL cells and incubate them with DNA, detecting IEL activity and the production of cytokine. Results:The activities of IL 2 and NK are all significantly higer than those of in control groups (P

Objective To utilize a reversed digital dorsal fascial vascular pedicled island fa scial flap with the dorsal branch of the proper d igital nerve to repair finger pulp defect.Methods Based on the features of blood supply of the dorsa l skin of the finger,the flap was designed on the proximal phalanx at the middle and distal segments and the dorsum of the proximal interphalangeal joint.The lateral margins of the fl ap did not cross the midlateral line,th e axial point was designed on the ulnar or the radial dorsum of the distal interphalangeal joint.The axis was paralleled to the longitudinal axis of finger.The dorsal branch of the pr oper digital nerve in the flap was sutured to the end of the proper digital nerve of injured site.From November 1997to April 2001,28pulp defects in 27cases were repaired with this method.E mergency operations were per-formed in 24cases and selective operations in 3.The flaps measured 1.8cm?1.5cm -2.5cm ?2.2cm and the length of pedicle was 1.5-2.2cm.Results All flaps survived completely.The p atients were followed up for 7to 32months,with an average o f 21months.The pulps after reconstr uction had good appearance,soft texture with wear-resisting.T here was no touch pain.Static two-point discrimination was 3.5-6.5mm.Conclusion The operation does not damage the major artery and nerve of the wounded fin ger.It is simple and safe,and can be done at one settin g.It does not interfere with the function of other digits.The sensation of the reconstructed pulp can be satisfactorily recovered.[