Objective To evaluate the effects of long-term intraperitoneal flurbiprofen axetil on the gastric mucosa and liver function in rats with bone cancer pain (BCP).Methods Thirty-six healthy female Sprague Drawley rats,weighing 200-250 g,were randomly divided into 6 groups (n =6 each):BCP + normal saline group (group BCP),BCP + flurbiprofen axetil 10 mg·kg-1 ·d-1 group (group BF10),BCP + flurbiprofen axetil 25 mg· kg-1 · d-1 group (group BF25),BCP + flurbiprofen axetil 50 mg· kg-1 · d-1 group (group BF50),flurbiprofen axetil 50 mg·kg-1 ·d-1 group (group F50),and sham operation + normal saline group (group S).BCP was induced by injecting Walker 256 mammary gland cancer cells into the bone marrow of the left tibia.In BCP and S groups,paw withdrawal threshold to mechanical stimulation (MWT) was measured at 1 day before inoculation of cancer cells (T0) and 3,5,7,10,14,17 and 21 days after inoculation (T1-7).The corresponding doses of flurbiprofen axetil or normal saline were administered intraperitoneally twice a day at 12 h intervals starting from T7 for 7 consecutive days.Blood samples were obtained from the abdominal aorta to determine the levels of alanine transaminase (ALT),aspartate transaminase (AST),total protein (TP),and albumin (ALB) in serum.The rats were then sacrificed and stomach and livers were removed for microscopic examination of pathological changes.The ulcer index (UI) was calculated.Results Compared with group S,MWT was significantly decreased at T2-7,no significant changes were found in the other parameters mentioned above in BCP group,the serum levels of TP and ALB were decreased in BF50 and F50 groups,and UI was increased in BF25,BF50 and F50 groups.Compared with BCP group,the serum levels of TP and ALB were decreased in BF50 and F50 groups,and UI was increased in BF25,BF50 and F50 groups.No significant changes were found in each parameter between BF50 and F50 groups.Microscopic examination showed that the epithelium of gastric mucous membrane became defect in partial region of the stomach and showed damage to gastric glands in the deep part and massive inflammatory cell infiltration in BF50 and F50 groups.Microscopic examination showed mild damage to the integrity and continuity of gastric mucosa and did not show any abnormality in livers in BF25 group.Conclusion Long-term intraperitoneal flurbiprofen axetil can cause damage to the gastric mucosa dose-dependently and has no effect on liver function in rats with BCP.

Objective:To investigate whether the overexpression of Oct4B1 gene induces epithelial mesenchymal transition in human colorectal cancer SW480 cells and its possible mechanism.Methods: Experimental group(SW480-Oct4B1):Transfection of SW480 cell lines in colorectal cancer with Oct4B1 overexpression plasmid;Control group(SW480-Oct4B1):negative control plasmid with G418 resistance.Stably transfected cell lines were obtained by G418 culture medium.The two groups were compared with:①Detection of Oct4B1 gene expression in stably transfected cell lines by RT-qPCR;②Scratches and Transwell assays were used to estimate migration and invasion;③Detection of EMT related markers E-cadherin,N-cadherin and Vimentin protein expression by Western blot assay;④Detection of Twist gene and protein expression by RT-PCR and Western blot assays.Results: The transient transfection was confirmed by RT-qPCR and the stable transfected cell lines were obtained from two groups of cells transfected with G418 culture medium.Compared with the control group:①RT-qPCR revealed increased expression of Oct4B1 gene in the experimental group(P<0.01);②Cell migration and invasion were significantly increased(P<0.01);③Epithelial marker:the expression of E-cadherin protein was significantly decreased (P<0.01),interstitial marker:the expression of N-cadherin and Vimentin protein was significantly increased (P<0.01);④Twist mRNA and protein expression were significantly increased(P<0.01).Conclusion: Overexpression of Oct4B1 gene can induce epithelial mesenchymal transition in human colorectal cancer SW480 cells,its molecular mechanism may be related to the promotion of Twist expression.

Objective To investigate the number, structure and demand of rehabilitation professionals hospitals above grade 2A in Jiangsu. Methods 176 hospitals above grade 2A in Jiangsu were investigated with questionnaire in the topics of general condition of department of rehabilitation medicine, structure of rehabilitation professionals and demand of rehabilitation professional. Results 64% of the hospitals has established department of rehabilitation. Rehabilitation professionals, such as the rehabilitation physician, therapists, nurses and engineer were in a total of 1657, and 231 professionals were needed further. Conclusion There are problems of lack of the department of rehabilitation medicine and rehabilitation professional, with the imbalance structure of professional in hospitals above grade 2A in Jiangsu.

OBJECTIVE：To provide reference for improving the quality sta ndard of Equisetum hyemale . METHODS ：Totally 10 batches of E. hyemale from different sites were collected as samples. TLC method was used to qualitatively identify kaempferol- 3-O-β-sophoroside. The contents of heavy metal ，aflatoxin，impurity，moisture，total ash ，acid-insoluble ash ，water-soluble extract and ethanol-soluble extract were determined according to supplementary provisions of Chinese Pharmacopoeia （2015 edition）. HPLC method was used to determine the content of kaempferol- 3-O-β-sophoroside in sample. HPLC fingerprint of water-soluble extract from E. hyemale was also established. RESULTS ：TLC identification showed that in the chromatogram of the test sample ， fluorescent spots with the same color were displayed on the corresponding positions of the chromatogram of substance control of kaempferol-3-O-β-sophoroside，and without interference from blank control. Among 10 batches of samples ，the contents of impurities were 0.19％-2.32％；the water contents were 10.12％-11.87％；the total ash contents were 6.67％-10.11％；the acid-insoluble ash contents were 1.34％-2.12％；the water-soluble extract contents were 9.17％-13.99％；the ethanol-soluble extract contents were 7.49％-13.68％，respectively. It is preliminarily proposed that the impurity content shall not exceed 3.00％；the total ash content shall not exceed 10.00％；the acid-insoluble ash content shall not exceed 2.50％；the water-soluble extract content shall not be less than 9.00％ ；the ethanol-soluble extract content shall not be less than 5.00％. Arsenic（0.064-0.225 mg/kg） 010815） was detected in 9 batches of samples ；cadmium（0.106-0.132 E-mail：cruise0303@163.com mg/kg）was detected in 6 batches of samples ；lead（0.221- 1.896 mg/kg）was detected in all samples ，but no mercury or rebecca aflatoxin was detected. The results of HPLC method met the relevant requirements of Chinese Pharmacopoeia . The content of kaempferol- 3-O-β-D-sophoroside in 10 batches of samples was 627.12-5 384.53 mg/kg，and the similarity of HPLC fingerprints of 10 batches of samples was more than 0.900. CONCLUSIONS ： A new qualitative and quantitative analysis method for kaempferol- 3-O-β-D-sophoroside was established ；the heavy metals ， aflatoxins，impurities and other items in E. hyemale were detected ；the limits of impurity ，ash and extract were determined. The established method is simple ，accurate and reproducible ，and can be used for quality control of E. hyemale .