【Objective】To observe the effect of electro-acupuncture(EA) and Banxia Xiexin Decoction(BXD) on motilin levels of functional dyspepsia(FD) rats.【Methods】 Sixty SD rats were randomized into normal control group,model group,mosapride citrate tablets(MCT,2.82 mg?kg-1?d-1) group,EA group,BXD(33g?kg-1?d-1) group,EA + BXD group.Except the normal control group,the rats with FD in other groups were induced by stimulating the rat tails with forceps clip. EA group received EA on bilateral acupoints of Zusanli(ST36) for consecutive 7 days.MTL content in rats plasma,antrum,jejunum,and hypothalamus was observed with radioimmunological method.【Results】MTL content decreased in the plasma,antrum,jejunum,and hypothalamus of the model group(P

BACKGROUND: Necrosis of femoral head induced by hormone is the iatrogenic disorder. It is doubt whether such problem is improved or not by increasing blood-flow volume in the local necrosis tissues.OBJECTIVE: To observe the effects of electric acupuncture on blood-flow volume in local tissues of femoral head so as to understand the mechanism of acupuncture on hormonal necrosis of femoral head.DESIGN: Randomized controlled study in which the experimental animals were taken as the objects.SETTING: Department of integrated traditional chinese and western medicine in a college of traditional chinese medicine.MATERIALS: The experiment was performed from May 2002 to June 2003in Animal Experimental Room of Clinical Hospital of Integrated Traditional Chinese and Western Medicine affiliated to Anhui College of Traditional Chinese Medicine. Thirty female and male(half-half) New Zealand white rabbits were employed, in clean grade, which were randomized into blank control, model group and treatment of electric acupuncture group.METHODS: The model of hormonal necrosis of femoral head in rabbits was prepared. In the treatment group, PCE-88A type controlled electric acupuncture apparatus developed by Institute of Acupuncture and Meridians and Collaterials in Anhui College of Traditional Chinese Medicine was applied on Huantiao(GB 30) and Biguan(ST 31) . No any treatment was applied in the model and blank groups.MAIN OUTCOME MEAURES: The curve of blood-flow volume in local necrosis tissues at the moment of acupuncture and 2 weeks after treatment.RESULTS: The blood-flow volume in local necrosis tissues at the moment of acupuncture in the treatment group(0.50 ±0.08) mL/g per minute was reduced significantly compared with that of blank control group[(8.63± 0. 83) mL/g per minute] and the model group[ (4.30 ±0. 89) mL/g per minute], indicating significant difference ( P ＜ 0.01). The blood-flow volume in local necrosis tissues 2 weeks after treatment[ (6. 19 ± 0.61) mL/g per minute] was remarkably increased compared with the blank control [ (8.33 ± 0.65) mL/g per minute] and the model group[ (4.44 ± 0. 72) mL/g per minute], indicating significant difference( P ＜ 0.01).CONCLUSION: Electric acupuncture can improve local blood supply in hormonal necrosis of femoral head immediately, which is probably related to the regulation in the net of nerve-endocrine-immunity.

Objective:To investigate the clinical characteristics,treatment and prognosis of primary pe-noscrotal extra-mammary Paget’s disease (PSPD).Methods:The clinical and pathological data of 22 cases of primary PSPD were retrospectively reviewed.Survival rate of the overall patients,the invasive patients,and the patients with positive surgical margin or negative surgical margin were analyzed with Ka-plan-Meier survival curve method.Results:Among all the patients with primary PSPD,the median age of onset was 64.5 (39 -84)years,the median time of disease duration was 40 (2 -300)months,and the median long diameter of lesion was 4.75 (1 -10)cm.In the study,12 patients (54.5%)were in pathological stage A1,6 patients (27.3%)were in pathological stage A2,and 4 patients (18.2%) were in pathological stage B.Scrotum and penile of most patients (n =12,54.5%)were involved,5 patients (22.7%)were scrotum only,and 5 patients (22.7%)were penile only.Erythema (n =18, 81.8%),itchiness (n =16,72.7%),ulcerate (n =12,54.5%),exudation (n =11,50.0%),and pain (n =4,18.2%)were the major manifestations.All the patients with primary PSPD were treated with wide surgical excision.The rate of invasive patients was 77.3% (n =17).Of them,6 patients had positive surgical margin.The surgical margins of non-invasive patients were all negative.Twelve patients exhibited local recurrence or/and metastases,and the status of surgical margins (P =0.015)and the depth of invasion (P =0.010)were important risk factors.Inguinal lymph nodes were generally in-volved.The difference of the delay of diagnosis between metastasis and non-metastasis was significant (P =0.040).The 5-year survival rates of the overall and invasive patients were 33.7% and 27.9%, respectively.The patients with positive surgical margin had poor prognosis.Conclusion:Primary PSPD, with the characteristics of long duration,high invasive tendency and high incidence of local recurrence or metastases,generally occurs in the elderly.Surgery should be performed at first.The delay of diagnosis, positive surgical margins and the involvement of inguinal lymph node are important risk factors.Biopsy, frozen section and inguinal lymph node biopsy (ILNB)can standardize diagnosis and treatment.

OBJECTIVE: To evaluate the predictive accuracy of the triage early warning score (TEWS) in the prognosis and emergency treatment for trauma patients admitted to the emergency department (ED). METHODS: A total of 456 trauma patients (>12 years old) admitted to ED at an education and research hospital in approximately 4 months were prospectively studied. Th e TEWS was recorded in all patients. Th e primary end-point was during 28 days and the emergency responses (such as cardiopulmonary resuscitation/electrical defibrillation, mechanical ventilation) in the ED. RESULTS: Patients with TEWS less than or equal to 9, from 10 to 13, or greater or equal to 14 had mortality rates of 0.98%, 52.63%, or 80%, respectively. An increase in 1 point within the range of 17-point TEWS would be associated with an odds ratio (OR) of 2.14 for death [95% confidence interval (CI): 1.759 to 2.604]. In predicting mortality rates during 28 days, the cut-point was greater than 8, the sensitivity was 87.10% (95% CI: 70.2% to 96.4%), the specificity was 92.47% (95% CI: 89.5% to 94.8%), and the areas under the receiver operating characteristic curves (AUCROC) was 0.929 (95% CI: 0.902 to 0.951). Th e AUCROC of TEWS in predicting the emergency responses for CPR/electrical defibrillation application or mechanical ventilation was 0.969 (95% CI: 0.949 to 0.983) or 0.897 (95% CI: 0.865 to 0.923), respectively. CONCLUSION TEWS is effective in predicting the prognosis and emergency treatment for trauma patients admitted to ED.
Area Under Curve ; Emergency Service, Hospital ; organization & administration ; Hospitalization ; Humans ; Prognosis ; Prospective Studies ; ROC Curve ; Sensitivity and Specificity ; Triage ; methods ; Wounds and Injuries ; diagnosis

Objective:Charcot-Marie-Tooth disease (CMT) comprises a group of clinically and genetically heterogeneous inherited neuropathies with an estimated prevalence of 1 in 2500. This study aimed to analyze the clinical and mutational characteristics of Chinese CMT patients with MFN2, BSCL2 and LRSAM1 variants.Methods:In this study, genetic analysis was performed in 206 Chinese patients at Chinese PLA General Hospital from December 2012 to March 2020 with clinical diagnosis of CMT, and reported variants of MFN2, BSCL2 and LRSAM1 related to CMT2.Results:We reported ten MFN2 mutations in ten unrelated patients (7 male, 3 female), two of whom had positive family history. Three novel mutations were detected including c.475-2A>G (splicing); c.687dupA (p.E230Rfs*16) and c.558dupT (p.S186fs). We reported three BSCL2 mutations of four unrelated patients, including c.461C>G (p.S154W), c.461C>T(p.S154L), and novel variants of c.1309G>C (p.A437P) and c.845C>T (p.A282V). Furthermore, two novel variants of LRSAM1, including c.1930G>T (p.G644C) and c.1178T>A (p.L393Q) were detected in two unrelated patients.Conclusion:Mutational spectrum of MFN2-, BSCL2-and LRSAM1-related CMT disease is expanded with the identification of novel variants in Chinese patients.

Objective To analyze the clinical features of secondary gout in glycogen storage disease type Ⅰ a (GSD Ⅰ a),so as to improve the awareness of this disease.Methods The clinical features,laboratory findings,treatments and prognosis of 5 GSD Ⅰ a patients with secondary gout who had been admitted to the Peking Union Medical College Hospital during 2006 to 2016 were collected and analyzed.GSD Ⅰ a was confirmed by liver biopsy and genotyping.Results Among the 5 patients (median age:27 years),3 were males and 2 were females.The mean age of gout onset was 17 ranging from 10 to 22 years old.The common manifestations of GSD included hepatomegaly since childhood,hypoglycemia,growth retardation,anemia,hyperlactacidemia and hyperlipidemia.All the 5 patients were complicated with gouty tophi and kidney stone.Gouty tophi and kidney stone were identified 3.8 years and 10.2 years after the first occurrence of articular symptoms,respectively.Renal damage occurred in 3 cases.All the patients underwent several therapeutic modalities including lifestyle intervention,allopurinol,and raw corn starch treatment.Conclusions Determination of the presence of primary disease should be performed actively for young-onset gout with early occurrence of gouty tophi.GSD should be suspected if there exist clinical manifestations like hepatomegaly,recurrent hypoglycemia,growth retardation.Early management of hyperuricemia and gout in GSD patients is important to prevent complications and improve prognosis.

OBJECTIVE: To investigate the current status on knowledge for unintentional injury and risky behavior among school-age children in Changsha, China, and to provide scientific evidence for the preventive strategies.
 METHODS: A cross-sectional study was conducted on 866 students who were between 6 and 12 years old in Changsha. Two primary schools were selected by stratified cluster random sampling from all primary schools of Changsha city to collect the information regarding knowledge for unintentional injury and risky behavior occurring in the 6-month period before the survey.
 RESULTS: The mean score for knowledge of unintentional injury was 11.83±2.38. The levels of knowledge for unintentional injury differed significantly in child's age, parents' education background and child's injury history (P<0.05). The child's knowledge level was correlated with child's age, mother's education, child's injury history. The mean score for risky behavior was 17.61±10.35. The levels of risky behavior differed significantly in child's gender, father's age to have the child, parents' marriage status, whom does/do child live with, child's injury history and medical history since the birthday (P<0.05). There was a linear regression relationship between risky behavior and child's injury history, parents' marriage status, child's gender. There was no significant correlation between knowledge and risky behavior (P>0.05).
 CONCLUSION It is a common phenomenon in school-age children who are lack of the knowledge for unintentional injury and risky behavior. This study provides useful information on the risk factors for unintentional injury and risky behavior, which would be significant for prevention program.
Accidents ; Child ; China ; Cross-Sectional Studies ; Humans ; Parents ; Risk Factors ; Risk-Taking ; Schools ; Students ; Surveys and Questionnaires ; Wounds and Injuries

The widespread application of next generation sequencing (NGS) in clinical settings has enabled testing, diagnosis, treatment and prevention of genetic diseases. However, many issues have arisen in the meanwhile. One of the most pressing issues is the lack of standards for reporting genetic test results across different service providers. The First Forum on Standards and Specifications for Clinical Genetic Testing was held to address the issue in Shenzhen, China, on October 28, 2017. Participants, including geneticists, clinicians, and representatives of genetic testing service providers, discussed problems of clinical genetic testing services across in China and shared opinions on principles, challenges, and standards for reporting clinical genetic test results. Here we summarize expert opinions presented at the seminar and report the consensus, which will serve as a basis for the development of standards and guidelines for reporting of clinical genetic testing results, in order to promote the standardization and regulation of genetic testing services in China.