1.In vitro evaluation of Ti-24Nb-4Zr-7.9Sn coated with microarc-oxidation film in cultured osteoblast cells
Zhengming LIU ; Bo GAO ; Lizhao ZHANG
Journal of Practical Stomatology 2001;0(03):-
Objective: To study the attachment, proliferation and ALP activity of osteoblasts on Ti-24Nb-4Zr-7.9Sn(TNZS) alloy surface treated by microarc oxidation (MAO). Methods: The surface roughness and energy of TNZS before and after MAO treated was examined by physicochemical methods, and pure titanium used as control. The primary cultured osteoblasts separated from calvarium of fetal rats were cultured and the third passage osteoblasts were seeded on 3 different surfaces of Ti, TNZS, MAO-TNZS discs. Biological assays were performed by MTT method. All data were statistically analyzed. Results: The surface energy and roughness of MAO-TNZS was higher than that of other groups; during the initial period of the cell adhesion on the materials, there were no differences among the three teams. But after 2 h, the cells adhesions on the surface of Mao-TNZS were higher than that on smooth surface and Ti (P
2.Pharmacokinetic Study on Hyperoside in Beagle's Dogs
Guo AI ; Zhengming HUANG ; Changxiao LIU
Chinese Herbal Medicines 2012;04(3):213-217
Objective To develop and validate a simple,rapid,sensitive,and reproducible HPLC method for determination of hyperoside in plasma of dogs and for the subsequent pharmacokinetic (PK) study.Methods An accurate and reproducible HPLC-UV method was developed and validated for the determination of hyperoside in plasma of dogs,using kaempferol as internal standard.The plasma samples of dogs following ig administration of hyperoside were analyzed for the detection of quercetin after enzymatic hydrolysis treatment with combined β-glucuronidase and sulphatase.The analytes were separated on a Diamonsil C18 column (250 mm × 4.6 mm,5 μm).The mobile phase consisted of methanol-buffer solution (0.1mol/L NH4Ac + 0.3 mmol/L EDTA-Na2)-acetic acid (60:40:1) and was delivered at a flow rate of 1mL/min.The UV detector was set at 370 nm and the column temperature was maintained at 35 ℃.The sample injection volume was 20 μL.Data were collected and analyzed using the ANASTAR software.PK parameters were calculated with DAS software (2.0).Results Linear relationships were validated over the range of 0.01-1μg/mL for hyperoside (r =0.9997).The intra-and inter-day precision values for all samples were within 10.0%,and the accuracies of intra-and inter-day assays were within the range of 92.4%-102.4%.The validated method was successfully used to determine the hyperoside concentration in plasma of dogs for up to 12 h,after a single ig administration (25 mg/kg).The mean PK parameters for male and female dogs were as follows:Cmax (0.18 ± 0.05) and (0.16 ± 0.05) μg/mL,AUC0-∞ (0.79 ± 0.34) and (0.86 ± 0.27) μg/(mL·h),t1/2(ka) (0.89 ± 0.41) and (0.88 ± 0.28) h,respectively.Statistical analysis on the PK of hyperoside in male and female groups showed that sex had no significant impact on the PK of hyperoside (P > 0.05).Conclusion The method is able and sufficient to be used in drug PK studies of hyperoside.
4.Study on ConA promoting phagocytosis, cytotoxicity and producing effectors of macrophages
Lifen GAO ; Junyan LIU ; Hongming TANG ; Xueju QU ; Zhengming JIANG
Immunological Journal 2001;(2):110-112
Objective To study how ConA actives macrophages in vivo to produce cytotoxic effectors and its phagocytic functions,and cytotoxicity. Methods ConA was intraperitoneally injected(ip). Cock red blood cells(cRBC) were used to evaluate MΦ phagocytic activity,and S180 cells as target cells to analyze MΦ dependent cytotoxicity(MTC).Nitric oxide(NO),TNF-α and IL-1 levels of MΦ cultural supernatant were measured using griess reagent,L929 cells MTT method and thymocytes proliferation test respectively. Results ConA could promote MΦ to phagocytize cRBC and kill S180 cells,enhance the production of such factors as NO,TNF-α and IL-1 by MΦ. There was significant difference compared with PBS control group(P<0.01). Conclusions ConA could stimulate MΦ to produce effectors, which mediate immune regulation of ConA to MΦ.
5.The effects of recombinant human bone morphogenetic protein2 and protein13 on the expression of proteoglycan gene in chondrocytes
Zhengming SUN ; Miao LIU ; Yingang ZHANG ; Yuping HUO
Chinese Journal of Rheumatology 2009;13(2):76-78
Objective By exploring the effects of recombinant human bone morphogenetic protein (rhBMP)2 and rhBMP13 on chondrocytes proteoglycan production and phenotype expression to establish the theoritical mechanisms for the treatment of disc degeneration with chondrocytes transplantation plus BMPs.Methods The dose-dependent effects of rhBMP2 and rhBMP13 on PG protein synthesis and gene expression were detected under different concentrations (0,25,125,and 625 ng/ml).The sulfated-glycosaminoglycan (s-GAG) in the culture media and the pericellular matrix was measured with a 1,9-dimethyl-methylene blue (DMMB) colorimetric assay.Reverse transcriptase polymerase chain reaction (RT-PCR) was performed respectively to quantify the relative abundance of aggrecan Mrna.Cell proliferation was examined by Hoechst Dye assay.Results All rhBMP2 and rhBMP13 in different concentrations could significantly increase s-GAG synthesis and gene expression in chondrocytes (P<0.05).And at the same concentration.rhBMP2 was more potent than rhBMP13 on s-GAG synthesis.Hoechst Dye assay showed neither rhBMP2 nor rhBMP13 had significant effect on cell proliferation.Conclusion rhBMP2 and rhBMP13 are able to upregulate s-GAG synthesis,in addition,rhBMP2 is more potent than rhBMP13 on aggrecan gene expression regulation,but rhBMP2 and rhBMP13 do not have significant effect on chondrocyte proliferation.
6.Shear bond strength of recycled metal brackets
Xinqiang LIU ; Zhengming LI ; Changbai MA ; Ding ZHANG ; Zhihui SUN
Chinese Journal of Tissue Engineering Research 2008;12(1):193-195
BACKGROUND: Bracket dropping is often seen in clinic. Can these shed brackets be used again; what should be done to treat these brackets; is the bond strength of these recycled brackets different from those new ones, these questions are often concerned by orthodontists in their clinic and are the purpose of our study as well.OBJECTIVE: To evaluate the shear bond strength and compare two base-cure methods of recycled brackets. DESIGN: Grouping control study. SITTING: Department of Orthodontics, the Affiliated Hospital of Medical College, Qingdao University; Department of Materials, Dental School, Beijing University. MATERIALS: The teeth were collected from Oral Surgery, the Affiliated Hospital of Medical College, Qingdao University. The age was from 11 to 19 years. The gender was half male and half female. Intact enamel of the crown was required, but decayed, fracture, tetracycline pigmentation teeth or fluorosis teeth were excluded. Patients provided the confirmed consent for this experiment.METHODS: The experiment was carried out in Stomatology College of Peaking University in December 2005. Thirty ex vivo teeth were selected randomly from those prepared enamel surfaces, used as sample for rebounding. The edgewise brackets of swallow-tailed brackets were bonded on each tooth and rebonded after 24 hours. The teeth were cleaned and divided randomly into 3 groups. There were 10 teeth in each group. Group 1 was bonded with new brackets; group 2 with recycled brackets whose adhesive remnant were burned and got rid of; group 3 with recycled brackets whose adhesive remnant in the groove of the base were stored. Bracket was provided by Standard edgewise, Orsu Company, Hangzhou, China. After 24 hours, the shear bond strength was tested with material testing machine (Autogragh, Shimadzu, Japan). Adhesive remnant index (ARI) was added up. MAIN OUTCOME MEASURES: Shear strength of old metal bracket after rebonding and adhesive remnant.RESULTS: ① Shear bond strength: The shear bond strengths were (10.094±3.158 9) in group 1, (10.266±2.406 0) in group 2 and (8.898±1.365 9) in group 3. There were no statistically significant differences among the three groups (P > 0.05). ② ARI scores: ARI scores were 51.7%, 58.3% and 35.0% in the three groups, respectively. There were statistically significant differences between group 2 and 3. CONCLUSION: In vitro study indicates that the recycled metal brackets can reach good bond strength and that maybe there is chemical bond between new and old adhesive.
7.Effect of orthodontic extraction treatment and non-extraction treatment on esthetical smile
Xinqiang LIU ; Maimaiti JUMANJIANG ; Yi CAO ; Zhengming LI ; Changbai MA
Chinese Journal of Medical Aesthetics and Cosmetology 2008;14(3):184-186
Objective To investigate the effect of orthodontic tooth extraction and non-extraction on dental arch width and esthetical smile. Methods 100 patients treated without extraction and 100 patients treated by 4 first-premolars extraction were selected. The study models of the patients were measured before and after the treatment and compared statistically. Measurements were made in the maxillary and mandibular canine regions from the most labial aspect of the buccal axial surfaces of the canine roots. Results Before treatment, maxillary and mandibular arch widths were the same between both groups (P>0.05). In non-extraction group, mandibular arch width of posttreatment was 0.88 mm larger than that of pretreatment (P<0.001), and maxillary arch width of posttreatment was 0.84 mm large (P<0.001). In extraction group, mandibular arch width of posttreatment was 1.64 mm larger than that of pretreatment (P<0.001), and maxillary arch width of posttreatment was 1.50 mm large (P<0.001). After treatment, the width of mandibular arch in the extraction group was 0.59 mm larger than that in the non-extraction group (P>0.05), while the width of maxillary arch in the extraction group was 0.10 mm less (P>0.05).Conclusion Both extraction treatment and non-extraction treatment do not result in narrower dental arch, but wider. The view that orthodontic extraction results in narrower arch widths and unaesthetic smiling is untenable.
8.The relationship between genotype of mouse and the immune response of hepatitis B vaccine
Liying MA ; Xi ZHONG ; Bingfei YUE ; Zhengming HE ; Shuanghuan LIU
Chinese Journal of Microbiology and Immunology 2008;28(11):1020-1023
Objective To test the potency of hepatitis B vaccine in China. Methods Two inbred strains(DBA/1 and BALB/c) and two NIH closes-colonies of mice were typed in the H-2 region by microcy-totoxicity method and PCR. Groups of mice of the tested strains were immunized with the same hepatitis B vaccine, the titre of anti-HBsAg antibody was analyzed by microplate, and the ED50 was then estimated by Karder method for each strain. Results Significant differences were found between potency estimates de-rived from assays using different strains of mice. Conclusion It is likely that the variation of immune re-sponse to hepatitis B vaccine in mice is correlative with the H-2 haplotype. In some special case, the bet-erozygosity in H-2 region found in NIH stock could influence the accuracy in such testing even a reference preparation of hepatitis B vaccine was used. Base on our experiment, to select an appropriate NIH stocks with the H-2q haplotype for potency testing of hepatitis B vaccine in China.
9.Research progress on the role of circRNA in gastrointestinal tumor
Ying XIONG ; Hanmin CHEN ; Wenqi YANG ; Zhichang LIU ; Zhengming ZHU
Chinese Journal of Clinical Oncology 2017;44(15):778-781
Circular RNA (circRNA) is a widely present endogenous noncoding RNA that is covalently bonded at the 3' and 5' ends via trans-splicing to form a closed-loop structure with high stability, biological evolutionary conservatism,and tissue expression specificity. CircRNA has important biological functions, such as playing the role of microRNA (miRNA) sponge, regulating the expression of a par-ent gene, and improving transcription and translation levels. In gastrointestinal tumor, circRNA mainly plays the role of miRNA sponge, which affects the proliferation, differentiation, apoptosis, and invasion of cancer cells. In addition, circRNA was specifically expressed in the tumor, and the expression level significantly differs from that of paracancer. Therefore, a highly conserved and stable circRNA se-quence is expected to be an early diagnosis and prognostic marker for gastrointestinal tumor. In this study, we review research advanc-es on circRNA and its relationship with gastrointestinal cancers (esophageal, gastric, colorectal, and liver cancers).
10.EFFECT OF MICROWAVE ELISA FOR IMMUNODIAGNOSIS OF SCHISTOSOMIASIS JAPONICA
Zhengming SU ; Min HU ; Hui HE ; Xingjian XU ; Jianbing LIU
Chinese Journal of Schistosomiasis Control 1989;0(03):-
0.05). Cross-reactivity was seen in 1 case of clonorchiasis sinensis by both the methods. Conclusion Microwave ELISA has the advantages of high sensitivity, specificity and rapidity.