Objective To detect the patients with IgA nephropathy by glycobiology and immunological technique. Methods An helia aspera lectin ELISA immunoassay was developed to discriminate the aberrantly glycosylated IgA in serum from normal IgA molecules by using HAA lectin, which reacts preferentially with the GalNAc of the terminal sugar chain, and AKP conjugated specific IgA antibody. Results The results given by A 405 nm values from 156 controls, 22 patients with IgAN and 19 patients with uncertain IgAN were 0.22?0.10,0.68?0.17 and 0.38?0.15, respectively. The significance between the controls and the patients with IgAN was observed ( P

Objective To study the effects of Traditional Chinese Medicine(TCM)therapy on shoulder hand syndrome(SHS).Methods 50 patients with SHS were divided into rehabilitation group and control group.The patients in rehabilitation group accepted physiotherapy and TCM therapy,which include marinate in traditional herb,pulsed electromagnetic therapy,massage and stimulate point.The patients in control group accepted physiotherapy only.They were evaluated after 1 month.Results The cured rate and the improved rate in rehabilitation group were higher than those in control group(P<0.05).Conclusion The TCM therapy is effective on SHS.

Objective To evaluate the clinical value of bone alkaline phosphatase in early diagnosis and treatment effect of chil-dren′rickets and to understand the incidence rate of rickets in children in the local region by the detection of neonatal bone alkaline phosphatase(NBAP).Methods 2 184 children with physical examination in our hospital from January to December 2013 were se-lected and divided into the infants,young children,preschool and school-age children groups according to age.The finger blood was collected for detecting the bone alkaline phosphatase in the peripheral blood by the bone alkaline phosphatase reagent kit provided by Beijing Jinyu diagnosis technology Co.,Ltd.Results Among detected 2 184 children,the detection rate of clinical rickets(NBAP>250 U/L)was 10.1 %,moreover the smaller the age,the higher the detection rate;the detection rate of subclinical rickets(NBAP 200~250 U/L)was 65.5%,indicating that most children in Baoji region were in sub-health state;the detection rate in spring and winter was higher than that in summer and autumn;the detection rate of NBAP had no gender difference.Conclusion Children are in the rapid growth and development period,easy to suffer from vitamin D deficiency,the activity detection of NBAP has important clinical value in the early diagnosis of rickets caused by vitamin D deficiency.Prevention should begin from perinatal period and NBAP should be timely detected in order to conduct early diagnosis and early treatment.

ObjectiveTo investigate the effect of daily living activity training on the self-care ability of children with cerebral palsy.Methods Sixty children with cerebral palsy were equally divided into control group and observation group. The former group were managed with rehabilitative therapy and the latter were given ADL training consecutively for 6 months apart from rehabilitative therapy.The two groups were compared in terms of score on ADL after 6 months treatment.Results There was no difference in the score on activities of daily living between the two groups before intervention (P>0.05),but the score after treatment in the obsertation group was significantly higher than that of the control group (P<0.01).Conclusion The ADL training is effective for improving the self-care ability of children with cerebral palsy and promoting the rehabilitation of children.

Spinal cord injury often resulted in disability. It is important to establish an ideal animal model to study spinal cord injury, its pathogenesis, regeneration and repair, etc. This article reviewed the establishment of models, including animals, method, characteristics, and discussed the frequently used models.

Objective To study the effects of Danshen Shujingwan on cervical cord compression in rats. Methods 60 healthy male SD rats of 8 weeks old were randomly divided into 6 groups: sham group (N group), model group (M group), DSW groups of high, medium and low dosages, and group treated with Jingfukang (J group), with 10 rats in each. The cervical cord compression models were established by transplanted silica gel. From 7 d after model establishments, the rats were respectively treated with drug by gavage for 30 d. They were observed pathologically. Results The extent and scope of damage were less in the treatment groups than in the model group (P<0.001), while the number of normal neurons remained more in the treatment groups (P<0.01).Conclusion DSW can decrease the damage after cervical spinal cord compression, protect the neurons from injuring.

Objective To detect the patients with IgA nephropathy by glycobilolgy and immunological technique.Methods An helia aspera lectin-ELISA immunoassay was developed to discriminate the aberrantly glycosylated IgA in serum from normal IgA molecules by using HAA lectin, which reacts preferentially with the GalNAc of the terminal sugar chain, and AKP conjugated specific IgA antibody.Results The results given by A405 nm values from 156 controls, 22 patients with IgAN and 19 patients with uncertain IgAN were 0.22±0.10, 0.68±0.17 and 0.38±0.15, respectively. The significance between the controls and the patients with IgAN was observed (P<0.001).Conclusion This assay is sensitive, specific and valuable for the diagnosis of patients with IgA nephropathy.

Objective To explore the application effect of reality cross-sectional assessment mode in the qualification certification of clinical nursing teachers. Methods A total of 218 nurses from Huai'an First Hospital, Affiliated to Nanjing Medical University who would be qualified by December 2015 were enrolled in the study. The control group contained 106 nurses who were working in internal medicine ward. The observation group included 112 nurses who were working in the surgical ward. The control group used the mode of theory combined with skill assessment for qualification certification. The observation group was qualified by theory in combination with reality cross-sectional assessment mode. Teaching quality, scores of theoretical examination and skills assessment, overall scores, as well as teaching satisfaction were compared between two groups. Results The score of clinical nursing teaching quality in the observation group was (95.52±2.43), which was higher than that in the control group (91.34±1.28). The difference was statistically significant (t=15.00, P<0.001). The observation group also showed significantly superiority in the theoretical examination scores (88.35±1.37), skills assessment scores (85.26±2.13) and overall scores (91.35±3.52) when compared with the control group [(83.17±1.42), (81.49±1.51), and (86.23±2.09)]; statistically significant differences were observed between two groups (t=62.87, 34.68, 30.07; P<0.001). The teaching satisfaction degree in the observation group was 94.59%, which was significantly higher than that (90.28%) in the control group (χ2=136.77, P<0.001). Conclusions The application of reality cross-sectional assessment in qualification certification of clinical nursing teachers can promote adequate teachers to stand out so as to optimize the clinical nursing teaching team.

[Objective]To summarize the research status and hotspots of tripterygium glycosides(TG),to provide reference basis for future in-depth research and clinical promotion of TG.[Methods]Computer retrieval of literature related to TG from January 2000 to June 2023 was conducted using the China National Knowledge Internet(CNKI),Wanfang Data,China Science and Technology Journal Database(VIP),China Biology Medicine disc(CBM).Use NoteExpress software to merge,duplicate check,and clean data,and use Excel software to construct an annual publication volume line chart.Visualize and analyze the author,organization,and keywords using VOSviewer 1.6.13 and CiteSpace 6.2 R2 software,and draw relevant graphs.[Results]A total of 1 752 articles were included.Since 2008,the number of publications has shown a decreasing trend in recent years after a gradual increase.A total of 4 186 authors were involved,including 75 core authors,forming a collaborative team represented by DING Ying,LI Zhenbin,LIN Na,CUI Ruiqin,CHEN Weidong and others.The main publishing institutions were the First Affiliated Hospital of He'nan University of Chinese Medicine and He'nan University of Chinese Medicine,followed by Dongzhimen Hospital of Beijing University of Chinese Medicine.The research institutions were relatively independent and scattered,mostly through cooperation between traditional Chinese medicine universities and their affiliated hospitals,with less cross regional cooperation.A total of six clusters of key words had been formed in the network diagram.The research focuses were mainly summarized as the clinical research on the application of TG combination drugs in kidney diseases such as diabetes nephropathy,purpura nephritis and nephrotic syndrome,rheumatic immune diseases such as rheumatoid arthritis,skin and mucosal diseases,and the experimental research on the toxicity and side effects of TG.In recent years,keywords that had emerged included dermatitis,eczema,mizolastin,inflammatory factors,immune function,tacrolimus,and so on.[Conclusion]The clinical efficacy and safety research of TG in kidney diseases,rheumatic immune diseases and other fields are hot topics.Dermatitis,eczema,and other skin and mucosal diseases,as well as the mechanism of TG action and inflammatory factors,may be the future research trends.There is still a need to strengthen cooperation among teams,strengthen basic research on the mechanism of TG action,and explore effective compatibility and detoxification methods,providing reference basis for the clinical application of TG in the future.

Objective: The purpose of this study was to investigate the state of depression and anxiety and assciated factors of back to school college students during the outbreak of COVID-19, so as to provide theoretical basis for emotional counseling and psychological crisis intervention after long term school closure due to epidemic outbreak. Methods: Cluster sampling was used to select the first batch of 2 697 back to school students in a high vocational college. The survey content includes basic information questionnaire, Depression Self rating Scale (SDS) and Anxiety Self rating Scale(SAS). Results: The detection rate of depression and anxiety was 31.8% and 10.4% respectively. Multivariate Logistic regression analysis showed that the risk of depression in female college students was 1.28 times higher than that in male students. The risk of depression among college students with family loss experience caused by the outbreak of COVID-19 was 1.30 times that of those without family loss experience caused by the outbreak of COVID-19. Outgoing personality(OR=0.62), neutral (OR=0.55) and mild temper(OR=0.46), moderate frequency of physical exercise per week(1-2 times:OR=0.73, 3-4 times:OR=0.65) were protective effects for depression in college students.Low frequency of breakfast were the risk effects for depression in college. The risk of anxiety among college students who was the only child in the family was 1.61 times higher than that who was not the only child in the family. The risk of anxiety among college students with family loss experience caused by the outbreak of COVID-19 was 1.45 times higher than that college students without family loss experience caused by the outbreak of COVID-19. Neutral temper, mild temper and physical exercise 1-2 times a week were associated with lower level of anxiety in college students(OR=0.67,0.56,0.67). Breakfast skipping (OR=2.03) was associated with higher levvel of anxiety in college students. Conclusion During the outbreak of COVID-19, the first batch of back to school college students presented high level of depression. Colleges are suggested to promptly carry out effective emotional relief and psychological intervention for students, especially those whose family suffered in COVID-19, the only child in the family, with poor breakfast eating habits, as well as breakfast skipping and lack of physical exercise.