Objective:To observe the change of apoptosis of nucleus pulposus cells cultured in vitro under hypoxic condition and different hydrostatic pressure.Methods:The human degenerative nucleus pulposus cells were cultured in vitro and randomly divided into 2 groups according to hypoxic condition:the hypoxia group and the normal oxygen partial pressure group.While the nucleus pulposus cells were randomly divided into three groups according to different hydrostatic pressure:the control group without pressure,the 50 kPa pressure group and the 100 kPa pressure group.The change of apoptosis of nucleus pulpo-sus cells was detected by methyl thiazolyl tetrazolium(MTT)method and terminal dexynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)assay.Results:As compared with that in normal oxygen partial pressure group,the proportion of survival nucleus pulposus cells in hypoxic group increased,while the number of apoptotic cells decreased.Under different hy-drostatic pressure,there was no statistically significant difference regarding the apoptotic positive rate of nucleus pulposus cells between the 50 kPa pressure group and the control group (P >0.05 ).However,the apoptosis positive rate in the 100 kPa pressure group increased by 3 1 .65%,compared with that in the control group,and the difference was statistically significant (P <0.01).Conclusions:Hypoxic condition,as well as low pressure condition,can reduce the occurrence of apoptosis of nu-cleus pulposus cells.

Echinococcosis is a zoonotic epidemic parasitic disease, which is caused by the infection with larvae of Echinococcus in humans, and the disease is also known as hydatid disease. The liver and lung are the most vulnerable organs. Aspergillus is a saprophytic fungus. Aspergillus infection is common in patients with immune deficiency. Pulmonary echinococcosis complicated with Aspergillus infection is very rare in patients with normal immune function. It is a case report in the literature at home and abroad. A case of bilateral isolated pulmonary echinococcosis complicated with Aspergillus infection with normal immune function and no other accompanying diseases admitted to Lanzhou University Second Hospital is reported as follows, and literature review is conducted in combination with Chinese and foreign literatures, so as to provide some reference value for diagnosis and treatment of this kind of disease.