The changes of plasma endothelin (ET) concentration and pulmonary arteriolar micromorphometric indexes including mean medial thickness in pulmonary arterioles (mMTPA), relative ratios of vessel wall area/total area(WA/TA), vessel lumen area/total area(EA/TA) on pulmonary arterioles, percentage of thickwalled peripheral lung vessels (TWPV) and mean density of pulmonary arterioles (mRPD) were observed in pulmonary hypertensive rats induced by hypoxia and hypercapnia. The results showed that all the values, appeared gradual changes with prolongation of time of hypoxia and hypercapnia. The tow-week group and four-week group exposed hypoxic and hypercapnic envirament compared with control group differences were significant(P

Objective To investigate the change of caspase-3 in rabbits after lung ischemia-reperfusion injury(LIRI) and the effect of puerarin(葛根素).Methods Thirty healthy rabbits used for unilateral lung ischemia-reperfusion model were randomly divided into 3 groups(each n=10): control group(C group),lung ischemia-reperfusion group(I/R group) and puerarin group.The activity of serum superoxide dismutase(SOD),the contents of serum malondialdehyde(MDA) and nitric oxide(NO),the wet to dry weight(W/D) ratio of lung tissue and the index of quantitative assessment of histological lung injury(IQA) were measured respectively in different groups;the pneumocyte apoptosis index(AI) was achieved by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling(TUNEL);caspase-3 protein and mRNA expression were studied by using in situ hybridization(ISH) and immunocytochemistry(IHC) techniques in the groups mentioned above.Results The activity of SOD and content of NO were significantly lower in I/R group than those in C group(both P

0.05) was observed. The protein expressions of TLR-4, NF-?B p65, HSP70 and ICAM-1mRNA in IR group were significantly increased as compared to C group and PD group, while those expressions in PD group were evidently higher than those in C group (all P

Objective To investigate the influence of injection carthamus tinctorius D. (1C) on the expression of intercellular adhesion molecule-1(ICAM-1) during the ischemia-reperfusion injury of lung (LIRI) in rabbits and its potential mechanism. Method Single lung ischemia-reperfusion animal model was induced in rabbits. A total of 30 Japanese white rabbits were randomly divided into sham-operation group (S group, n =10), ischemia-reperfusion group (I/R group, re = 10) and ischemia-reperfusion plus 1C group (1C group, n = 10) .The rabbits of 1C group received 1C 2.0 ml/kg injected intravenously just at 20 min before ligation of artery involved and the same dose of 1C instantly at the initiation of reperfusion. Malondialdehyde (MDA) , superoxide dismutase (SOD) and xanthine oxidase(XO) in serum were measured. The lung tissue was sampled and assayed wet/dry weight ratio (W/D), contents of myeloperoxidase (MPO) at the end of the experiment, and ultrastructure changes were observed under electron microscope. The expression of ICAM-1 was measured by using immunohistochemistry(IHC) . snd one-way ANOVA was used for statistical analysis. Results In I/R group, serum XO and MDA increased and SOD decreased, whereas the same pattern of changes but less magnitude happened in 1C group ( P < 0.01). The values of W/D and MPO were much higher in I/R group, but lower in 1C group. Under electron microscope, the ultrastructure of lung tissue showed pathological changes in the rabbits of I/R group,and these changes were greatly attenuated in the rabbits of 1C group . The IHC showed that ICAM - 1 in lung tissue of I / R group was (2.94±0.48) which was significantly higher than that of 1C group(1.75 (P < 0.01). Conclusions Injection Carthamus tinctorius D. may meliorate the ischemia-reperfusion injury of lung by way of suppressing the expression of ICAM-1, inhibiting neutrophil aggregation, lowering oxygen free radical level and decreasing lipid peroxidation.

Personalized information service has become an important interest in medical library due to the rapid development of Internet technology. The SOA framework-based personalized information service system architecture was proposed by analyzing the contents and characteristics of personalized information service in medical library, which is characterized by standards, loose couple, low cost modification and operating maintenance, and can thus ensure both personalized information service and daily professional activities in medical library.

OBJECTIVE: To improve the checking management of outpatient pharmacy and to reduce the error rates of checking process. METHODS: With the data from "Army No 1" hospital information system, to designe dynamic checking table ordered by fixed location of each drugs in containers, and pending prescription transfer database is established to manage unconfirmed expired prescriptions. RESULTS & CONCLUSIONS: The order of name in the stock table of drugs is identified to the position of each drug in containers. There is no requirement for incorporate check-in operation of the same kind of drugs from various manufactories and identification of their locations. Taken advantage of computer technology, this checking program is more time-saving, simple and easy, and significantly increases the accuracy of results.

AIM: To investigate the effects of safflor injection(SI) on expression of cyclooxygenase-2 mRNA during lung ischemia/reperfusion injury(PIRI) in rabbits.METHODS: Rabbit lung model of ischemia/reperfusion injury was constructed in vivo.The rabbits were randomly divided into three groups: sham-operation group(group S),ischemia-reperfusion group(group I/R) and ischemia/reperfusion plus safflor injection group(group SI).The lung tissue sampled at the end of the experiment was assayed for wet/dry weight ratio(W/D),injured alveoli rate(IAR) and observed ultrastructure changes under electron microscope.The expressions of COX-1 and COX-2 were measured by immunohistochemistry(IHC).The expression of COX-1 mRNA and COX-2 mRNA were observed by in situ hybridization(ISH).RESULTS: The value of W/D and IAR was much higher in I/R group,but decreased in SI group.Electron microscope showed obvious ultrastructure injury brought by PIRI in I/R group,which was greatly attenuated in SI group.The IHC and ISH demonstrated that COX-2 and COX-2 expressions in pulmonary tissue of I/R group were significantly higher than those in S group(P

AIM: To clarify the role of nitric oxide (NO) system in development of chronic hypoxic hypercapnic pulmonary hepertension. METHODS: Male Sprague-Dawley rats were randomly divided into control group and hypoxic hypercapnic group. NO content of plasma was determined, constitutive nitric oxide synthase (cNOS) and inducible nitric oxide synthase (iNOS) were examined using the technique of immunohistochemistry, expression of cNOS mRNA and iNOS mRNA of arteriole were detected by in situ hybridization. RESULTS: Plasma NO concentration, cNOS activity and cNOS mRNA expression in arteriole of chronic hypoxic hypecapnic group were significantly lower than that of control group ( P

AIM: To investigate the expression of soluble guanylate cyclase protein and its mRNA in rat pulmonary artery after exposure to hypoxia and hypercapnia. METHODS: Male Sprague-Dawley rats were randomly split into 4 group, which were hypoxic hypercapnic (HH 1 week, HH 2 weeks, HH 4 weeks) group and control group, to copy pulmonary hypertensive animal model. The expression of sGC? 1 and ? 1 subunits protein of medial and small pulmonary artery was performed by immunohistochemistry with a polycolonal antibody. In situ hybridization was performed on the rat lung tissue using sGC oligonuclear probe to assay the expression of sGC? 1 subunit mRNA. RESULTS: The sGC? 1 and ? 1 subunits protein and sGC? 1 subunit mRNA were faint staining in the pulmonary small and medium artery in HH1 week, HH 2 weeks and HH 4 weeks groups compared with control group (all P

AIM: To study the role and the mechani sm of heme oxygenas/endogenous carbon monoxide on nitric oxide synthase/nitric oxide system in rats with pulmo nary hypertension induced by hypoxic hypercapnia. METHODS: Spr ague-Dawley rats w ere randomly divided into three groups: control group (A group),hypoxic hypercap n ic group (B group), hypoxic hypercapnia+hemin group (C group). Blood CO concentr at ion (COHb%),NO concentration,HO-1 activity, iNOS, cNOS in blood serum and lung h omogenate were measured, respectively. RESULTS: ① mPAP and RV /(LV+S) of B g roup were significantly higher than those of A and C group( P