Objective To analyze the effects of information literacy on participation in scientific research for medical students.Methods The self-administered questionnaire was used to survey the general characteristics,information literacy,participation in scientific research projects of junior college students,undergraduates,and graduate students who majored in medical specialties such as clinical medicine.The participation in scientific research includes the following statuses:those who did not participate in any project (group A),those only participating in the teachers' projects (group B),the team members of college students' projects (group C),and the leaders of college students' projects (group D).The data were tested using x2 test,one-way analysis of variance,and multinomial logistic regression analysis in SPSS 17.0 for windows.Results The survey recycled 1 236 copies of effective questionnaires.About 39.6％ (489/1 236) students participated in scientific research.The scores of information consciousness,information ability,and information morality,and total scores had statistically significant differences among the four groups (P=0.000).The scores of information ability and total scores of group D (n=73) were higher than those of group A (n=747),B (n=93),and C (n=323) (P＜0.05),respectively.With group A as a reference category,the scores of information ability of group D,C,and B had statistically significant partial regression coefficients (P=0.005,P=0.000,P=0.000,respectively),as well as total scores (all P=0.000).Conclusion High information literacy may be one of factors that promote students to participate in college students' scientific research projects.Various comprehensive measures should be taken to cultivate students' information literacy and improve students' scientific research ability.

Medical inorganic chemistry is one of the most important basic courses to bioinformatics specialty,according to the requirements of curriculum;the paper introduces a series of reform and practice in inorganic chemistry teaching content,teaching method and form.These stimulate the students'study interest,enhance educational effect,and let this curriculum serve professional curriculums.

Objective To establish the method to analyze the contents of Costunolide and Dehydrocostus Lactone in Tibetan medicineLiuwei Muxiang Pill.Methods HPLC method was used; the XDB-C18 column (4.6 mm × 50 mm, 1.8μm) was adopted;the mobile phase was methanol- water (60∶40);the flow rate was 0.8 mL/min;UV detection was 225 nm.Results The linear range of Costunolide and Dehydrocostus Lactone was 0.235 5-1.413μg and 0.291 3-1.748μg. The average recoveries were 99.25% and 99.41%, respectively.Conclusion The method is accurate, reliable, stable and with good repeatability, which provides the basis for the quality evaluation of Tibetan medicineLiuwei Muxiang Pill.

The paper first gives an account of the historical backgroumd of the formation of the World Trade Organization and the existing 6 basic principles of it. Then it explains the principles and extent of the opening of the health sector to the outside world and the specific promises made by China in the negotiations involved in its accession to the WTO. Finally the paper speculortes about the effects of Chinas entry into the WTO on domestic hospitals and suggests corresponding strategies.

Objective To analyze the effects of medical undergraduates' creativity tendency on participation in scientific research.Methods The self-administered questionnaire was used to survey the general characteristics,Williams creativity tendency,participation in scientific research projects of the junior students who majored in 10 medical specialties such as clinical medicine.The participation in scientific research includes the following statuses:those who do not participate in the scientific research project(NPSR,group A),those only participating in the teachers' scientific research project (PTSR,group B),those participating in college students' scientific research project as team members (TMSR,group C),and those participating in college students' scientific research project as project leaders(PLSR,group D).The differences of four dimensions scores and total scores of creativity tendency among four groups were tested using one-way analysis of variance.The effects of creativity tendency on participation in scientific research were tested with multinomial logistic regression analysis.Results The survey recycled 2 286 copies of effective questionnaires.About 39.1％(894/2 286) students participated in scientific research.The students were divided into A group(n=1 392),B group (n=108),C group(n=626),D group(n=160).The scores of adventure,curiosity,imagination,chal lenge and total scores had statistically significant differences among the four groups(P=0.000).The scores of adventure,curiosity,challenge and total scores of group C and D were higher than those of group A(P＜0.05),respectively.With NPSR as a reference category,the scores of adventure(P=0.011),imagination (P=0.023),challenge (P=0.025) of PLSR had statistically significant partial regression coefficients,and the scores of adventure(P=0.009),curiosity(P=0.002) of TMSR had statistically significant partial regression coefficients.Conclusion High creativity tendency may be one of influencing factors that promote students to participate in college students' scientific research project.Various comprehensive measures should be taken to cultivate students' creativity and improve students' scientific research ability.

BACKGROUND: Clinically, in patients undergoing hematopoietic stem cell transplantation (HSCT), human cytomegalovirus (HCMV) can be associated with delayed platelet engraftment, phenotypically abnormal peripheral blood leukocytes, and graft rejection, possibly through a direct viral effect on hematopoietic progenitor cells after HCMV infection. OBJECTIVE: To investigate the inhibitory effect of ganciclovir (GCV) on proliferation of colony forming unit (CFU) granulocyte-macrophage (CFU-GM), CFU-erythroid (CFU-E), CFU T-lymphocyte (CFU-TL), CFU-multipotential (CFU-Mix) and CFU-megakaryocyte (CFU-Mk) progenitor cells of cord blood (CB) and the protective effects on them. DESIGN: Contrast observational study.SETTING: Department of Molecular Biology, Affiliated Hospital of Luzhou Medical College.PARTICIPANTS: A total of 20 cord blood (CB) samples (with 10 mL for each sample) from fetal umbilical vein of normal term spontaneous delivery neonates were provided by the Department of Gynaecology and Obstetrics, Affiliated Hospital of Luzhou Medical College. All the patients were informed and agreed with the experiment.METHODS: The experiment was carried out in the Department of Molecular Biology, Affiliated Hospital of Luzhou Medical College from June 2004 to December 2006. Colony forming unit-assay was applied to observe the suppression effect of HCMV-AD169 strain on CFU-GM, CFU-E, CFU-TL, CFU-Mix and CFU-Mk of CB with the presence of GCV. The techniques of polymerase chain reaction (PCR) and fluorescence quantification PCR were used to demonstrate the existence of HCMV-AD169 DNA in the colony cells of cultured CFU-GM, CFU-E, CFU-TL, CFU-Mix and CFU-Mk. Normal progenitor cells culture system was regarded as blank control group; normal progenitor cells culture system with inactivated HCMV fluid as inactivated (IV) control group.MAIN OUTCOME MEASURES: ① The number and maintaining duration of colonies of cultured progenitor cells were counted by using a light inverted phase contrast microscope. ② The techniques of PCR and fluorescence quantification PCR were used to demonstrate the existence of HCMV-AD169 DNA in the colony cells of cultured progenitor cells.RESULTS: ① Number and lasting time of colonies: The numbers of CFU-GM, CFU-E, CFU-TL, CFU-Mix and CFU-Mk colonies in the HCMV infection group were significantly less than those in the blank control group (P < 0.01). The maintaining duration of colonies in the HCMV infection group was significantly shorter than that in the blank control group (P < 0.01). HCMV-DNA copies of colony cells of GCV group decreased significantly by using fluorescence quantification PCR compared with HCMV group (P < 0.01), while negative in blank control and inactivated control in CFU-MK and CFU-Mix. ② CFU Growth rate: The Growth rate of colonies was 37.4%, 74.2%, 40.1%, 67.4% and 38.9% of CFU-GM, CFU-E, CFU-TL, CFU-Mix and FU-MK, respectively. ③ CFU-HCMV-AD169 DNA: Fluorescence quantification PCR showed that nucleonic acid content of progenitor cells after GCV-affected HCMV infection was decreased as compared with that after HCMV infection (P < 0.01).CONCLUSION: The differentiation and proliferation of CFU-GM, CFU-E, CFU-TL, CFU-Mix and CFU-Mk are significantly inhibited after infected with CMV-AD169 strain. The growth of hematopoietic progenitor cell after HCMV-AD169 infection is promoted by GCV, which suggests that GCV has an effect of anti-HCMV in vitro.

Objective To analyze the effects of achievement motivation and family environ-ment of undergraduates majoring in preventive medicine on creativity tendency. Methods The self-administered questionnaire was used to survey the general characteristics , achievement motivation , family environment, and Williams creativity tendency of undergraduates (n=243) from grade 1 to grade 5 majoring in preventive medicine in a medical college. The effects of achievement motivation and family environment on creativity tendency were tested with multiple linear regression analysis. SPSS 17.0 software was used for statistical analysis. The indexes between boys and girls was compared with group t test, and single factor analysis of variance was made for comparison between grades, SNK method was used to test and compare two groups; linear correlation analysis was made to inspect the correlation between the two indicators, and multiple linear regression analysis method was used for further analysis. Results The survey recycled 226 copies of effective questionnaires. The scores of achievement motivation, family environment, and Williams creativity tendency and all dimen-sions had no statistically significant difference among the five grades, or between the male and female (P>0.05). The positive influence factors of creativity tendency and its dimensions were pursuing success, the in-formative property, the entertaining property and organizational property, the negative influence factor was avoiding failure. R2 was 0.094-0.265 (P=0.000-0.048). Conclusion Achievement motivation and family environment of undergraduates majoring in preventive medicine may have posi-tive effects on creativity tendency. Various comprehensive measures should be taken to improve family environment and enhance achievement motivation, and to promote to increase creativity tendency, and to cultivate students' creativity and improve students' scientific research ability.

AIM　The purpose is to construct D-hydantoinase genetic engineering strain for the purpose of the industrial production of D-p-hydroxyphenylglycine. METHODS　D-hydantoinase gene was created from Pseudomonas putida 9801 by PCR technique and inserted into pMD18-T vector. The recombinant plasmid was transformed into several Escherichia coli strains. The positive transformants with D-hydantoinase activity were obtained by the two step screening, digoxigenin DNA labeling in situ hybridization and D-hydantoinase activity assay. RESULTS　The D-hydantoinase activity of the genetic engineering strain E.coli BL21/pMD-dht was 1700 U*L-1 and increased as high as 8 times compared with those of wild-type strain Pseudomonas putida 9801. The subunit molecular weight of recombinant D-hydantoinase was about 53 kDa measured by SDS-PAGE. The amount of the recombinant D-hydantoinase was about 20 percent of total bacterial soluble proteins. CONCLUSION　The genetic engineering strain E.coli BL21/pMD-dht possesses the initial industrial production prospects.

Objective To set up a method of rapid culture,drug susceptibility testing and identification for Mycobacterium Tuberculosis.Methods Using the method by ourselves made and examined the standard strains and clinical strains of mycobacterium,research to the culture, the drug susceptibility testing and the identification of group in mycobacterium by this method compared with the method of Lowenstein-Jensen (L-J) method. To make sure the characteristic identification of group in mycobacterium.Results Detection time of culture of this method was 19.13 d earlier than that of L-J method. The positive rate of the method was higher than that of the L-J method. The applied concentration of each drug was: INH:0.1 ?g/ml, RFP:1 ?g/ml, EB:2 ?g/ml, AMK:2 ?g/ml、LVFX:2 ?g/ml、PNB 200 ?g/ml、TCH 2.5 ?g/ml respectively. The report time of the method was 7～10 d. It was 18～21 d shorter than that of the L-J method (28 days).Conclusion The method shortened significantly the report time of culture、drug susceptibility testing and identification of group in mycobacterium. The rate of positive was raised.This method was economic, practical and suitable to expansion and application in the substrata units.

Objective To establish the method for determining the content of cryptotanshinone and tanshinone ⅡA in Zhiwei Fuwei Wan. Methods The determination of cryptotanshinone and tanshinone ⅡA was performed on Symmetry C18 column with mobile phase consisted of acetonitrile-0.2%phosphate (60∶40) at flow rate of 1.0 mL/min. The detection wavelength was set at 262 nm. Results The linear ranges of cryptotanshinone and tanshinone ⅡA were 0.081 4-0.407 2 μg (r=0.999 7) and 0.163 6-0.81 8 μg (r=0.999 5) respectively, the average recoveries were 99.70% (RSD=1.37%) and 99.22% (RSD=0.94%) respectively. Conclusion The method is simple and rapid for the quality control of the product.