Objective To investigate the effects of islet B cells of rat on the differentiation of T-helper lymphocytes and its potential mechanism.Methods Forty male SD rats were equally divided into five groups.Group-A:render diabetes by injection of streptozotocin (STZ);Group-B:OVA sensitized and then OVA challenged to render asthma;Group-C:render diabetes and then OVA sensitized,and challenged;Group-D:render diabetes,and then insulin subcutaneous injection,OVA sensitized,and challenged;Group-E:nondiabetes,nonsensitized,only OVA challenged.Bronchoalveolar lavage (BAL) was performed in rats from each group 24h after challenged and BAL fluid was used to detect IFN-? and IL-4.Blood glucose was estimated,total and differential leukocyte counts were carried out in blood samples and C-peptide concentration was tested in serum.Results The C-peptide concentration in serum was decreased significantly,while INF-? expression was higher,and IL-4 expression was markedly lower in BAL fluid in both group-A and group-C when compared with that of controls,respectively.Compared with group-C,IL-4 expression was increased and INF-? were significantly lower,respectively in BAL fluid of group-D after insulin subcutaneous injection.Conclusion Islet B cells of rat might modulate the differentiation of T-helper lymphocyte.

Objective To observe the expression and distribution of the basic fibroblast growth factor(bFGF)in lung through chronic obstructive pulmonary disease(COPD)rat models and to investigate the potential role of bFGF in the mechanism for airway remodeling in COPD.Methods Forty male,8 weeks old Wistar rats were divided into groups randomly:COPD group;bFGF interfere in COPD group;anti-bFGF interfere in COPD group,and control group.COPD models were established by intratracheal instillation of lipopolysacchairde(LPS)twice in a month and exposure to cigarette smoking daily and in order to contrast respectively instillate particular quantity bFGF and anti-bFGF into different COPD model group from caudal vein in every week.After these models became true,observe the pathologic alteration of small airways,arterioles and pulmonary alveolus by hematoxylin and eosin stain Van-Gieson +elastic fibers stain.At the same time,the thickness of the smooth muscle and collagen in bronchi and pulmonary arterioles were measured by computer image analyzer;also the protein and gene relative content of bFGF as well as the effects of antibody on them were observed by Histostaining and ISH(in situ hybridization)detection.Results There was a significant increase in the thickness of the smooth muscle and collagen in bronchi of bFGF interfere in COPD group compared with that of control group and COPD group(P

Objective To investigate the ability of osteogenesis, repaired effects and possible mechanism of tissue engineered bone made in an approach of bionics as a transplantation biomaterial to repair a segmental defect of long bone. Methods HA/?-TCP was composed with PDLLA and then composed with rhBMP-2 and collagen of typeⅠ. The combined biomaterial was put in common culture with osteoblasts harvested from periosteum of rabbit and vascular endothelial cells from kidney of rabbit then transplanted this tissue engineered bone to total segmental periosteum-bone defect of 1.5 cm in the rabbits radius which were investigated 4, 8 and 12 weeks after operation respectively. Investigation of the bone defect was made by means of gross observation, X-ray examination, histology of HE and Masson staining, image pattern analysis, scanning electron microscopy, EDAX. Results In gross observation, the implantations were adhered to the host bone well in four weeks, the implantations was bony healed with host bone in eight weeks, and some of the implantations were replaced by new formation bone in 12 weeks. In histological examination of four weeks after operation, lamellar bone was found, and eight weeks after operation, implant was incorporated to host bone end by end through cortical bone, and new bone marrow was found to invade into the implant. Furthermore, the outer part of implant was completely substituted by new cortical bone 12 weeks after operation. In addition, the histological study pointed out that the new bone arranged in type of various bands which were in subsequent transition. There is significant difference between 4 weeks and 8 weeks, and 4 weeks and 12 weeks, but no significant difference between 8 weeks and 12 weeks of the quantity of new bone. The ratio of calcium to phosphorus in the transplants tended to approach that in the host cortical bone along with the period of time after operation. Conclusion Satisfied effects of remodeling appeared after this tissue engineered bone composed by bionics was transplanted to the segmental defect of long bone. The mechanism of bone regeneration was endochondral ossification.

Objective To evaluate the role of interleukin-5 (IL-5) in the pathogenesis of allergic asthma, we observed the effects of recombinant human (rh) IL-5 inhalation on changes of activity and number of circulating eosinophils in allergic asthmatics, as well as concentrations of serum IgE.Methods A randomized double-blind, placebo-controlled study design was employed in each subject which acted as his or her own control. Eight nonsmoking asthmatics were enrolled in this study. All paients had mild atopic asthma, with baseline forced expiratory volume in 1 second (FEV1) greater than 70% of predicted value, requiring only intermittent use inhaled β2-agonists. Each paitent had one or more documented positive skin prick test responses to aeroallergens. None had received inhaled or oral corticoseroids in the previous 3 months. Each subject inhaled 10 μg of rhIL-5 (Genzyme Co., Boston, MA) in vehicle (0.1% bovine serum albumin in 0.9% saline) or vehicle only as a 0.5 ml nebulized solution. At least 4 weeks were allowed to elapse between the two inhalation, and the order of inhalation of rhIL-5 or vehicle was randomized. Measurements of total nuclear blood cell counts and cell differntial counts, and concentrations of eosinophil cationic protein (ECP) were performed before, and at 2, 24, and 48 h after the inhalation of rhIL-5 or vehicle. Levels of ECP in serum were determined with commercially available ECP Fluoroimmunoassay kits (Pharmacia AB, Uppsals, Sweden) according to the procedures recommended by the manufactures. All data were presented as mean±standard error of mean. Statistical analysis was done by repeated measures analysis of variance (ANOVA) for data conforming to a normal distribution, and by Friedman's test for those data with a nonparametric distribution.Results Eosinophil numbers and ECP levels within the control group did not appear to change from baseline at any time throughout the study. Eosinophil numbers from baseline (3.6±1.1×105/ml) to 6.3±1.2×105/ml (P<0.01) at 24 h, and to 5.7±0.9×105/ml (P<0.01) at 48 h after IL-5 inhalation. Accompanying this significant blood eosinophilia was a significant elevation of serum ECP levels. Compared with baseline value (6.3±1.1 pg/ml), inhalation of IL-5 lead to ECP levels in crease with time, reaching a significant hight extent at 24 h (17.6±2.8 pg/ml, P<0.01); And this elevated ECP levels lasted at least 48 h (18.1±2.9 pg/ml, P<0.01). However, IL-5 inhalation had no significant effect of levels of serum total IgE.Conclusions The findings in the present study provided direct evidence the IL-5 not only induced a significant blood eosinophilia, but also resulted in the activation of circulating eosinophils. Our data further support the importance of IL-5 in the pathogenesis of brochial asthma in human.

Objective:To improve the understanding of primary pulmonary lymphoepithelioma-like carcinoma (PPLELC).Methods:A retrospective case series study was conducted. The clinical data of 31 patients with PPLELC who were admitted to the First Affiliated Hospital of Guangxi Medical University from January 2012 to June 2023 were retrospectively analyzed, and their clinical features were summarized. The correlations of organ metastasis, tumor stage, serum tumor markers, lactate dehydrogenase, and albumin with survival time were analyzed.Results:Among the 31 patients, 13 (41.9%) were male and 18 (58.1%) were female, aged (50±9) years old, with no smoking history in 24 cases (77.4%). The common clinical manifestations were cough(24 cases, 77.4%) and sputum (19 cases, 61.3%), and 7 patients (22.6%) were detected by physical examination; 24 cases (77.4%) had elevated levels of serum tumor markers, and the rest of the 7 cases (22.6%) had normal levels of various tumor markers. All of the patients had a single lesion, with a predominance of the right middle lung (8 cases, 25.8%), and 23 cases (74.2%) had lymph node metastasis. Immunohistochemical detection showed that the positive rate of CK was 67.7% (21/31), and the positive rates of squamous cell carcinoma markers CK5/6, p63 and p40 were 90.3% (28/31), 80.6% (25/31) and 77.4% (24/31), respectively. The positive rate of EBER in situ hybridization detection was 85.2% (23/27). Genetic testing showed 6 cases had epidermal growth factor receptor (EGFR) mutation. The median survival time [ M ( Q1, Q3)] of the groups without lymph node metastasis and with lymph node metastasis was 33.0 months (7.3 months, 9.3 months) and 19.0 months (7.0 months, 27.0 months), and the difference was statistically significant ( P < 0.001). The median survival time of patients with stage Ⅰ-Ⅱ and with stage Ⅲ-Ⅳ was 20.0 months (12.5 months, 42.0 months) and 18.5 months (6.5 months, 38.5 months), and the difference was statistically significant ( P = 0.002). One stage Ⅰ A patient was treated with surgery alone and survived at 92 months of follow-up. Ten cases were treated with immunotherapy and had a good outcome. Conclusions:PPLELC is prevalent in non-smokers, the lesions are mostly in the right middle lung, and it is easily misdiagnosed as squamous cell carcinoma. The positive EBER in situ hybridization detection can help the diagnosis; lymph node metastasis is common. Tumor stage, lymph node metastasis and CYFRA21-1 level may be correlated with the survival of patients. The patients can benefit from immunotherapy, and anti-angiogenic therapy combined with chemotherapy is an optional treatment regimen.