Objective: To explore the anticancer mechanism of Stellera chamaejasme L. abstracts with water (SCLA). Methods: SCLA drugserum was derived after different time from mice which was orally peradministrated with different dose of SCLA. the effects of the serum on the roliferation of human gastric adnocacinoma SCG7901 cells were observed with MTT assay and clone formation methods.Results: After treatment with the drugserum derived at 1,2,3h from mice pertreated with SCLA(5,10 and 20g?kg -1 ), the MTT formazan and cone formation of gastric adnocacinoma SGC7901 cells were decreased significantly. SCLA from the mice which were given the drugserum 2h later was more effective than others. Conclusions: SCLA had a remarkable inhibitory effects on proliferation of gastric adnocacinoma SGC7901 cells, which may be the main anticancer mechanisms of SCLA.

OBJECTIVE: To establish an HPLC method for the determination of 8-O-acetyl-shanzhiside methylester in Tibet medicinal Lamiophlomis rotata.METHODS: HPLC analysis was performed on Symmetry C18(150 mm?4.6 mm,5 ?m) column with the mixture of acetonitrile-water(15∶85) served as the mobile phase.The detection wavelength was set at 234 nm.RESULTS:The linear range of 8-O-acetyl-shanzhiside methylester was 1.97～19.68 ?g?mL-1(r=0.999 6) and its average recovery was 99.53%(RSD=1.73%,n=9).CONCLUSION: The method is simple,accurate and specific,and it applies reference for quality evaluation and utilization of Tibet medicinal L.rotata.

OBJECTIVE:To study the protective effects of Ginkgolides(GL)on cerebral ischemia in experimental rodents. METHODS:The models of decapitative mice and acute incomplete cerebral ischemic(AICI)rats were used.The gasp duration of decapitative mice and cerebral index,water content of brain,cerebral capillary permeability and hemorrheological parameters in rats were determined.RESULTS:Ginkgolides could prolong gasp duration of decapitative mice,increase the cerebral index and the water content of brain,inhibit capillary permeability,improve hemorrheological parameters and decrease blood viscosi?ty.CONCLUSION:Ginkgolides may antagonize the damage of cerebral ischemia.

The antiumor activity of a new podophyllotoxin spin-labeled derivative, 4 -C 4 "-( 2 ", 2", C", 6"-tetramethyl- 1 "-piperidinyoxy ) amino]-4'-demethylepipodophyllotoxin ( GP-7 ) was studied. It was found that the growth of transplanted mouse tumors S180, HePS and Lewis lung cancer was markedly inhibited by GP-7. At a dose of 7.5-20 mg/kg, the inhibition rates of it against Sl80, HePS and Lewis lung cancer were 36.0-58.4, 29.6-60.0, and 27.2-46.5 % respectively. The toxicity of GP-7 was low, as indicated by the LD50 value of 231.2 mg/kg which was 3.3 times higher than that of etoposide ( VP-16 ) . On the other hand, the effects of GP-7 on spleen index and thymus index of mice bearing S180 tumor were remarkably lower than that of VP-16. In vitro GP-7 exhibited marked inhibition effects against L1210 and SGC-7901 cells. After exposure of L1210 cells to GP-7 and VP-16 5 mg/L for 24 h, the. inhibition rates were 75.5 and 73.6 %. after exposure of SGC-7901 cells to GP-7 and VP-16 5 mg/L for 72 h, the inhibition rates were 81.4 and 84.2 %. The new podophyllotoxin derivative GP-7 was similar to its structure analogues, clinical drug VP-16, in antitumor activity, while the toxicity of it was much lower than that of VP-16.

In order to study the enhancement of immune functions and autologous tumor-killing (ATX) activity by kappa-selenocarrageenan (KSC) in mice bearing sarcoma 180, the effects of KSC and/or Cyclophosphamide (Cy) on natural killer(NK) activity, lymphokine activated killer(LAK) activity, the production of interleukin-2 (IL-2), ATK activity and the growth of sarcoma 180 (S180) were observed. The results showed that KSC promoted NK activity, LAK activity and ATK activity in vivo, increased IL-2 production at 40mg/kg ?d x 9d. It also enhanced the antitumor action of Cy (20mg/kg?d x 9d) and offsetted the inhibition of Cy on immunocompetent cells. The ATK activity in splenocytes of SI80-bearing mice could be induced and augmented by recombinant interleukin-2 (rIL-2) in vitro. In conclution, KSC had a up-regulating effect on immune functions and ATK activity in tumor-bearing mice, therefore, can be used as a biological response modifier (BRM) in cancer biotherapy.

History and current situation of hospital preparation in our country and practical demands of pharmaceutical industry were analyzed. With the implementation of GMP in pharmaceutical industry and enhanced drug administration, lots of problems were exposed. The scale of hospital preparation was decreasing year by year, though it still stayed at a predominate position in hospital works currently.

Objective:To establish an HPLC method for the content determination of salvianolic acid B in Ansheng Yizhi cap-sules. Methods:A Hypersil ODS2 C18 column(150 mm × 4. 6 mm,5 μm) was used and methanol-water-formic acid (40∶60∶1) was used as the mobile phase. The detection wavelength was at 286 nm. The flow rate was 1. 0 ml·min-1 and the sample size was 10 μl. Results:The calibration curve of salvianolic acid B was linear within the range 7. 75-77. 51 μg·ml-1(r=0. 999 6). The average re-covery was 98. 17%(RSD=1. 79%, n=6). Conclusion:The method is simple, accurate and repeatable, which can be used in the quality control of Ansheng Yizhi capsules.

Object To explore, on the whole, the anticancer activity and mechanism of the aqueous extract of Ruixiang Langdu (Stellera chamaejasme L.) (SCLA). Methods Mice were given different i.g. doses of SCLA and their serum collected at different intervals after drug administration. The effects of the serum on the proliferation of mouse L 1210 leukemic cells were observed with MTT assay, clone formation and incorporation of [ 3H] TdR into DNA of the cells. Results Serum SCLA collected 1, 2, 4, 8 h after administration of 3, 6, and 12 g/kg SCLA showed significant decrease of MTT formazans and clone formation. Serum SCLA collected 2 h after drug administration showed more strong inhibition of cancer cell proliferation. It also inhibited the incorporation of [ 3H] TdR into DNA of L 1210 cells. Conclusion SCLA showed a remarkable inhibitory effect on proliferation and DNA synthesis of cancer cells cultured in vitro, which may be the main anticancer mechanisms of SCLA.

Object To study the augmentation of the T activated killer (T AK) cell proliferating and tumor killing activities of the polysaccharide fraction from Rhodiola kirilowii (Regel) Regel, Lycium barbarum L., Hedysarum polybotrys Hand. Mazz., Glehnia littoralis F. Schmidt ex Miq., Rehmannia glutinosa (Gaert.) Libosch. ex Fisch. et Mey. and Aloe barbadensis Mill. in vitro. Methods The T AK cells were induced by anti CD 3 antibody (CD 3McAb) and rIL 2 from human peripheral blood mononuclear cells (PBMC). The effects of the above six plant polysaccharides (1～100 ?g/mL) on the proliferation of T AK cell, the cytotoxicity to Raji cells and L 1210 cells, and the IL 2 receptor (IL 2R) expression in T AK cells were determined. Results The six polysaccharides alone had no obvious effect on the proliferation of T AK cells. In the presence of rIL 2 and CD 3McAb, they could reinforce the proliferation of T AK cells and its tumor killing activities against Raji cells and L 1210 cells at a different extent, and a 21%～68% increase of IL 2R expression in T AK cells was observed. Conclusion The plant polysaccharides significantly enhance the proliferation and the tumoricidal activities of T AK cells and the enhancing actions related to the increase of IL 2R expression.

AIM: To study the influence of ginkgolides on expression of inducible nitric oxide synthase (iNOS) in rats. METHODS: Middle cerebral artery occlusion(MCAO) model was induced by ligating internal carotid artery of Sprague Dawley(SD) rats.In this model,the behavioral deficits performance of SD rats was analyzed after given ginkgolides and nimo dipin, respectively, and iNOS gene expression changes in MCAO group were investigated. RESULTS:The expression of iNOS was increased in MCAO models and inhibited in ginkgolides groups. CONCLUSION:Ginkgolides have inhibitive efficacy of iNOS on cerebral ischemia and can reduce brain damage.