1.A study on medical image fusion.
Er-hu ZHANG ; Zheng-zhong BIAN
Chinese Journal of Medical Instrumentation 2002;26(5):356-372
Five algorithms with its advantages and disadvantage for medical image fusion are analyzed. Four kinds of quantitative evaluation criteria for the quality of image fusion algorithms are proposed and these will give us some guidance for future research.
Algorithms
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Humans
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Image Enhancement
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methods
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Image Interpretation, Computer-Assisted
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methods
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Image Processing, Computer-Assisted
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methods
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Information Storage and Retrieval
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methods
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Magnetic Resonance Imaging
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Software
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Tomography, X-Ray Computed
2.A DSP-based design method for detecting fetal heart rate signals.
Xiao-feng YANG ; Peng LI ; Da-long ZHANG ; Zheng-zhong BIAN
Chinese Journal of Medical Instrumentation 2006;30(6):413-415
The paper presents the development and design of an ultrasonic Doppler fetal heart rate monitoring system based on DSP, and explains the realization of a algorithm for detecting fetal heart rate. Clinical practice has proved that this system is able to pick up the real-time fetal heart rate correctly and rapidly.
Algorithms
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Fetal Monitoring
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instrumentation
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methods
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Heart Rate, Fetal
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physiology
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Humans
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Sensitivity and Specificity
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Signal Processing, Computer-Assisted
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Ultrasonography, Doppler
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instrumentation
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methods
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Ultrasonography, Prenatal
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instrumentation
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methods
3.Advances in research of ketamine addiction mechanism.
Wei-Li LIU ; Shi-Zhong BIAN ; Zhen-Lun GU ; Xiao-Gang JIANG ; Zheng-Hong QIN
Journal of Forensic Medicine 2009;25(3):200-207
Ketamine is a phencyclidine derivative acting primarily as a noncompetitive antagonist of N-methyl-D-aspartate (NMDA) excitatory glutamate receptors. As a common intravenous anaesthetic in clinic, it is also increasingly abused because of its hallucination and addiction effects. Based on the pharmacological and toxicologic characteristics of ketamine and the acknowledged addiction mechanism of other abused drugs, this article reviews the possible addiction mechanism of the ketamine in the aspects of its enhanced effects and reward systems, the anatomic structures, the related receptors and the individual differences.
Anesthetics, Dissociative/adverse effects*
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Animals
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Brain/drug effects*
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Humans
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Illicit Drugs
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Ketamine/adverse effects*
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Mental Disorders/chemically induced*
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Rats
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Receptors, Dopamine/drug effects*
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Receptors, N-Methyl-D-Aspartate/drug effects*
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Substance-Related Disorders
4.A multifrequency phase-controlled driving system for ultrasound therapy.
Qi-jun DAI ; Qin-wu ZHOU ; Xiao-zhou XU ; Ke-yong Ou YANG ; Zheng-zhong BIAN
Chinese Journal of Medical Instrumentation 2002;26(4):256-258
Ultrasound therapy is a new type of therapy technology, there are hyperthermia and high intensity focused ultrasound surgery (HIFUS). Compared with a single frequency system, mutiple frequency system has an additional function to combine power patterns of different frequencies. This function increases the availability of power patterns to treat tumors of various shapes and depths. Therefore, we have in this article proposed a system with the ability to drive ultrasonic phased arrays of multiple resonant frequencies for ultrasound hyperthermia and HIFUS. The results show that this system is able to (1) drive multi-element applicators or phased arrays of a single resonant frequency through the multichannel power amplifiers, (2) concurrently drive transducer with different resonant frequencies, (3) adjust the relative phase and output power of each channel for the scanning ultrasonic focus, and (4) operate each channel with good output stability.
Equipment Design
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Humans
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Neoplasms
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therapy
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Transducers
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Ultrasonic Therapy
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instrumentation
6.A novel approach of low-frequency ultrasonic naked plasmid gene delivery and its assessment.
Wei WANG ; Zheng-Zhong BIAN ; Yong-Jie WU ; Ya-Lin MIAO
Biomedical and Environmental Sciences 2005;18(2):87-95
OBJECTIVETo deliver the naked genes into cells through the bioeffects of cell membrane porous produced by low-frequency ultrasound (US) and to investigate the safety by determining the threshold of cell damage and membrane permeability.
METHODSThe suspension of red cells from chickens, rabbits, rats, and S180 cells was exposed to calibrated US field with different parameters in still and flowing state. Laser scanning confocal microscopy, fluorescent microscopy, scanning electron microscopy, flow cytometry and spectrophotometry were used to examine cell morphology, membrane permeability, enzymes, free radicals, naked gene expression efficiency, threshold of cell damage and cell viability.
RESULTSThe plasmid of green fluorescent protein (GFP) as a reporter gene was delivered into S180 cells under optimal conditions without cell damage and cytotoxicity. The transfection rate was (35.83 +/- 2.53)% (n = 6) in viable cells, and the cell viability was (90.17 +/- 1.47)% (n = 6). Also, malondialdehyde, hydroxyl free radical, alkaline phosphatase, and acid phosphatase showed a S-shaped growth model (r = 0.98 +/- 0.01) in response to the permeability change and alteration of cell morphology. The constant E of energy accumulation in US delivery at 90% cell viability was an optimal control factor, and at 80% cell viability was the damage threshold.
CONCLUSIONUS under optimal conditions is a versatile gene therapy tool. The intensity of GFP expression in US group has a higher fluorescent peak than that in AVV-GFP group and control group (P < 0.001). The optimal gene uptakes, expression of gene and safety depend on E, which can be applied to control gene delivery efficiency in combination with other parameters. The results are helpful for development of a novel clinical naked gene therapeutic system and non-hyperthermia cancer therapeutic system.
Animals ; Cell Membrane ; chemistry ; metabolism ; ultrastructure ; Cell Survival ; genetics ; Cells, Cultured ; Chickens ; DNA Damage ; genetics ; Free Radicals ; metabolism ; Genes, Reporter ; genetics ; Green Fluorescent Proteins ; genetics ; Malondialdehyde ; metabolism ; Permeability ; Plasmids ; genetics ; Porosity ; Rabbits ; Rats ; Risk Assessment ; Transfection ; methods ; Ultrasonics
7.Comparison of sperm parameters between male adults at different altitudes.
Liang-kuan YU ; Jun-hao GUI ; Jing FENG ; Bian HU ; Guo-xiang HUANG ; Zheng WANG ; Rui WANG ; Wu-zhong YU ; Dong-hui QIU
National Journal of Andrology 2007;13(2):122-124
OBJECTIVETo investigate the influence of long-term anoxic exposure on the sperm function of male adults at different altitudes.
METHODSA total of 28 male adults that had stayed at the altitude of 5 340 m for 1-3 years were included as a high-altitude group (HAG), 34 at the mean altitude of 3 800 m for 2-5 years as a middle-altitude group (MAG) and 31 permanently at the altitude of 1 300 m as controls. Semen specimens were collected and the real-time semen analysis was performed by using computer-assisted semen analysis (CASA) system.
RESULTSThe sperm density, VCL, VSL, VAP and LIN in the HAG were (51.12 +/- 14.61) x 10(6)/ ml, (48.17 +/- 13. 52) microm/s, (32.64 +/- 6.70) microm/s, (41.21 +/- 9.32) microm/s and 52.24 +/- 8.14, respectively, significantly lower than those of the control (P < 0.01 or P < 0.05). Compared with the control group, there was a progressive decrease in sperm concentration, sperm motility rate, VSL, VCL, LIN, VAP and ALH in the MAG.
CONCLUSIONThe higher the altitude, the more obvious was the negative effect of anoxic exposure on the sperm function of male adults.
Adult ; Altitude ; Control Groups ; Diagnosis, Computer-Assisted ; Humans ; Male ; Sperm Count ; Sperm Motility ; Spermatozoa ; physiology
8.Survival and disease burden trend analysis of occupational pneumoconiosis from 1963 to 2020 in Shizuishan City.
Yan TONG ; Yun Yan KONG ; Hao BIAN ; Jian Zhong ZHENG ; Yong Jun WU ; Yue ZHANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2022;40(5):341-347
Objective: To understand the survival status and its influencing factors of occupational pneumoconiosis patients in Shizuishan City, and to analyze the disease burden of occupational pneumoconiosis and its trend, so as to provide scientific basis for formulating comprehensive prevention and treatment measures of occupational pneumoconiosis. Methods: A retrospective survey was conducted during July to December 2020 to explore the survival status of occupational pneumoconiosis patients who had been reported from 1963 to 2020 in Shizuishan City. The Kaplan-Meier method and Life-table method were used for survival analysis, and Cox proportional hazards regression model was used to analyze the influencing factors of survival time. The disability adjusted life years (DALY) was applied to analyze the disease burden of occupational pneumoconiosis and its temporal trend. Results: From 1963 to 2020, a total of 3263 cases of occupational pneumoconiosis were reported in Shizuishan City, of which 1467 died, so that the fatality rate was 44.96%. The median survival time was 26.71 years, average age of death was (70.55±10.92) years old. There were significant differences in the survival rates of occupational pneumoconiosis patients among different types, diagnosis age, exposure time, industry, initial diagnosis stage and whether upgraded (P<0.05) . As the survival time increased, the survival rate of patients decreased gradually. When the survival time was ≥50 years, the cumulative survival rate of patients was 4.20%. Cox regression analysis suggested that the type of pneumoconiosis, industry, diagnosis age, exposure time, initial diagnosis stage and whether upgraded were the influencing factors for the survival time of patients with occupational pneumoconiosis (P<0.05) . The total DALY attributable to occupational pneumoconiosis from 1963 to 2020 in Shizuishan City was 48026.65 person years, of which the years of life lost (YLL) was 15155.39 person years, and the average YLL was 10.33 years/person, and the years lost due to disability (YLD) was 32871.26 person years, and the average YLD was 10.07 years/person. The DALY attributed to coal worker's pneumoconiosis and silicosis were 39408.51 person years and 6565.02 person years, respectively, and they accounted for 82.06% and 13.67% of the total disease burden in Shizuishan City, respectively. The DALY caused by occupational pneumoconiosis in the age group of 40-49 years old and the first diagnosis of stage I occupational pneumoconiosis were higher, which were 20899.71 and 36231.97 person years, respectively. The average YLL and average YLD showed a volatility downtrend over time. Conclusion: The disease burden of occupational pneumoconiosis cannot be ignored in Shizuishan City, and timely targeted measures should be taken for key populations and key industries. It is recommended that life-cycle health management and hierarchical medical should be taken to improve the life quality of patients and prolong their lifes.
Adult
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Aged
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Aged, 80 and over
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Anthracosis
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China/epidemiology*
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Coal Mining
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Cost of Illness
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Humans
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Middle Aged
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Pneumoconiosis/epidemiology*
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Retrospective Studies
9.Combination immunotherapy of glioblastoma with dendritic cell cancer vaccines,anti-PD-1 and poly I:C
Ping ZHU ; Shi-You LI ; Jin DING ; Zhou FEI ; Sheng-Nan SUN ; Zhao-Hui ZHENG ; Ding WEI ; Jun JIANG ; Jin-Lin MIAO ; San-Zhong LI ; Xing LUO ; Kui ZHANG ; Bin WANG ; Kun ZHANG ; Su PU ; Qian-Ting WANG ; Xin-Yue ZHANG ; Gao-Liu WEN ; Jun O.LIU ; Thomas-John AUGUST ; Huijie BIAN ; Zhi-Nan CHEN ; You-Wen HE
Journal of Pharmaceutical Analysis 2023;13(6):616-624
Glioblastoma(GBM)is a lethal cancer with limited therapeutic options.Dendritic cell(DC)-based cancer vaccines provide a promising approach for GBM treatment.Clinical studies suggest that other immu-notherapeutic agents may be combined with DC vaccines to further enhance antitumor activity.Here,we report a GBM case with combination immunotherapy consisting of DC vaccines,anti-programmed death-1(anti-PD-1)and poly I:C as well as the chemotherapeutic agent cyclophosphamide that was integrated with standard chemoradiation therapy,and the patient remained disease-free for 69 months.The patient received DC vaccines loaded with multiple forms of tumor antigens,including mRNA-tumor associated antigens(TAA),mRNA-neoantigens,and hypochlorous acid(HOCl)-oxidized tumor lysates.Furthermore,mRNA-TAAAs were modified with a novel TriVac technology that fuses TAAs with a destabilization domain and inserts TAAs into full-length lysosomal associated membrane protein-1 to enhance major histo-compatibility complex(MHC)class Ⅰ and Ⅱ antigen presentation.The treatment consisted of 42 DC cancer vaccine infusions,26 anti-PD-1 antibody nivolumab administrations and 126 poly I:C injections for DC infusions.The patient also received 28 doses of cyclophosphamide for depletion of regulatory T cells.No immunotherapy-related adverse events were observed during the treatment.Robust antitumor CD4+and CD8+T-cell responses were detected.The patient remains free of disease progression.This is the first case report on the combination of the above three agents to treat glioblastoma patients.Our results suggest that integrated combination immunotherapy is safe and feasible for long-term treatment in this patient.A large-scale trial to validate these findings is warranted.
10.Whether Syndrome Differentiation Affects Treatment Result: Study Protocol of MaZiRenWan () for Functional Constipation in A Randomized Controlled Trial.
Chung-Wah CHENG ; Li ZHANG ; Chen ZHAO ; Linda Ld ZHONG ; Li-Jie SHI ; Liang DAI ; Rui ZHENG ; Jing CHEN ; Ge LI ; Jing-Bo ZHAI ; Wai KUN ; Ai-Ping LU ; Hong-Cai SHANG ; Zhao-Xiang BIAN
Chinese journal of integrative medicine 2019;25(3):175-181
BACKGROUND:
Syndrome is one of the most important concepts in Chinese medicine (CM) theory. However, it was not well accounted in most of randomized controlled trials (RCTs).
OBJECTIVES:
To determine whether CM syndrome differentiation affects the treatment results, functional constipation (FC) was selected as a target disease, and MaZiRenWan (, MZRW), a classic CM formula commonly used for constipation with excessive heat syndrome, was selected for study.
METHODS:
It is an 18-week prospective double-blinded, doubledummy RCT, including 2-week run-in, 8-week treatment and 8-week post treatment follow-up. A total of 120 FC patients diagnosed as excessive heat syndrome will be recruited from the First Teaching Hospital of Tianjin University of Traditional Chinese Medicine and the Baokang Affiliated Hospital of Tianjin University of Traditional Chinese Medicine. Patients will be randomly allocated into fixed MZRW (f_MZRW) granule group, modified MZRW (m_MZRW) granule group or bisacodyl group. For m_MZRW group, no more than two herbal granules can be added according to the syndrome differentiation for individual participants. The primary end point is the mean of complete spontaneous bowel movements (CSBMs) per week during the treatment period. Secondary end points include mean of CSBMs per week during follow-up, stool form, global symptom improvement, constipation and constipation-related symptoms assessment, CM syndrome change, and reported adverse events.
DISCUSSION
This trial is designed to evaluate the effectiveness of these three interventions for FC patients with the CM syndrome of excessive heat, and to determine the change of CM syndrome and the progress of disease during the treatment course. The results are important to explore whether syndrome differentiation is important for the therapeutic effect of a formula on a disease. [Trial registration: Chinese Clinical Trial Registry (Reg No. ChiCTR-TRC-13003742); protocol version: MZRW/NSFC-81173363 (2015.05.04)].
Constipation
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diagnosis
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drug therapy
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Double-Blind Method
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Drugs, Chinese Herbal
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therapeutic use
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Humans
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Medicine, Chinese Traditional
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Outcome Assessment (Health Care)
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Prospective Studies
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Randomized Controlled Trials as Topic