ObjectiveTo study the the simplified rehabilitation procedures characternized with early ambulation for the acute stroke hemiplegic patients without disturbance of consciousness or cognition. Methods40 patients were divided into 2 groups: observed group (20 cases) and control group (20 cases). The patients of observed group were trained with the simplified rehabilitation procedures, while the patients of control group were trained with routine rehabilitation training program. ResultsThe time and costs of rehabilititation training decreased in the observed group compared with those of control group （P＜0.05）. ConclusionThe simplified rehabilitation procedures may benefite the acute stroke hemiplegic patients without disturbance of consciousness and cognition.

Cardiopulmonary resuscitation is an important part of standardized residency training. There are certain flaws in the various commonly used training and assessment mode. In the exploration of the new mode, "step-by-step" training helps to learn skills step by step. "Low intensity and high frequency" training not only focuses on key points and difficulties, and lightens the burden of the teachers and residents, but also can improve the training effect through repeated reinforcement. Combining different training methods organically to establish a "diversified" training mode can make full use of the advantages of different methods and make up for each other's shortcomings. In addition, the "veto power" should be introduced into the assessment. Scenario simulation with role play may be an effective way to train teamwork ability, but it still needs further exploration.

Three-dimensional (3D) laparoscopic surgery has been developed rapidly in China since 2012.Based on mature two-dimensional (2D) laparoscopic surgery,3D laparoscopic surgery has the same technique as 2D,with similar indication as 2D as well.However,3D laparoscopic surgery can offer better depth description,more accurate spatial location,and less error in operation.It might offer better visional experience if hybrid with virtual reality or with 4K in the future.But in current status,laparoscopy with 3D technique is only a technique with innovation,but not a revolutionary technique.

Objective The purpose of this study was to determine if parafascicular nucleus of thalamus is involved in the nociceptive stimulation evoked by coronary artery occlusion-induced acute myocardial ischemia and to investigate the effect of fentanyl on this nociceptive stimulation. Methods Male SD rats weighing 260-300 g were operated upon under general anesthesia with intraperitoneal urethane (1.2 g ? kg -1 ) and local infiltration of the skin incision. The discharges of pain-sensitive neurons (PSN) were recorded for 20 seconds every 5 min using single-barrel glass electrode before and after the coronary artery occlusion ( CAO) . The study was divided into 3 groups: group Ⅰ CAO alone ( n = 9); group Ⅱ CAO + fentanyl ( n = 6) : fentanyl 0.01 mg ?kg-1 was administered iv 15 min after CAO; group Ⅲ CAO + fentanyl + naloxone ( n = 6) : naloxone 0.04 mg? kg-1 was administered iv 15 min after intravenous fentanyl administration. Results The discharge frequency was significantly increased following CAO and peaked within 5-10 min after CAO and maintained for 60 min. The increased frequency of nociceptive discharge was significantly inhibited within 10 min after fentanyl and intravenous naloxone could completely antagonize the inhibitory effect of fentanyl. Conclusion Visceral pain can be evoked by acute myocardial ischemia induced by coronary artery occlusion. Thalamic parafascicular nucleus is involved in the perception of nociception in CNS. Opioid receptors play a critical role in the modulation of the nociception.

The study of tumors' proteomics is a research hotspot in China and abroad in recent years.The development of theory and technology in proteomics has provided new ideas and research field for cancer research.This paper reviewed the commonly used technical means of proteomics and its research progress on diagnosis,therapy and other aspects of pancreatic carcinoma.

Objective To evaluate the effects of different time courses and depths of acute normovolemic hemodilution (ANH) with hematocrit (Hct) on injury to the mucous membrane of small intestines in rabbits.Methods Thirty-two adult rabbits,weighing 2.0-2.5 kg,were randomly divided into 4 groups (n=8 each) using a random number table:control group (group C),Hct 18％ group (H1 group),Hct 15％ group (H2 group) and Hct 12％ group (H3 group).The animals were anesthetized with iv 20％ urethane 4 ml/kg,tracheostomized and mechanically ventilated.Blood samples were taken from the left femoral artery.Blood withdrawn from the femoral artery =2 ×body weight× body fluid per kilogram × (initial Hct-target Hct) ÷ (initial Hct+target Hct),and was simultaneously replaced with the equal volume of 6％ hydroxyethyl starch 130/0.4 infused over 30 min via the left femoral vein until the target Hct was achieved in H1,H2 and H3 groups.At 30 min of stabilization after puncture (T0) and 2,4 and 8 h after ANH (T1-3),blood samples were obtained from the superior mesenteric artery and vein for blood gas analysis and for determination of plasma concentrations of tumor necrosis factor-α (TNF-α) in the superior mesenteric vein.Oxygen extraction rate (ERO2) was calculated.Small intestinal mucosal tissues were obtained at T3 to observe the pathological changes with light microscope.Results Compared with those at T0,the plasma concentrations of TNF-α at T3 and ERO2 at T1-3 were significantly increased in H1 group,and the plasma concentrations of TNF-α at T3 and ERO2 at T1-3 were increased in H2 and H3 groups.The injury to the mucous membrane of small intestine was mild in group H1,and was severe in H2 and H3 groups.Conclusion ANH performed with 6％ hydroxyethyl starch causes injury to the mucous membrane of small intestine when Hct is 18％ and the time course ≥ 8 h,and when Hct ≤ 15％ and the time course ≥ 2 h in the rabbits.

Objective:To lay foundation for the functional studies of programmed cell death 5 ( PDCD5 ) and develop new technical pathway for bioinformatics analysis of human functional genes. Methods:Using PDCD5 as the target molecule, intensive bioinformatics analysis of the nucleic acid and protein sequences were conducted. Data mining and comprehensive analysis by sequence against database similarity searching, ortholog structure comparison, expression profile analysis and gene “neighbor” listing were performed. Results: Two human putative pseudogenes on chromosomes 12 and 5, and one mouse putative pseudogene on chromosome 1 were identified. The methanobacterium thermoautotrophicum ortholog was classified as the same fold as ubiquitin and ribosomal protein S13. The C. elegans ortholog, ubiquitin and IAP (inhibitor of apoptosis proteins) belonged to the same expression profile cluster. This cluster was related to biosynthesis and protein synthesis. PDCD5 orthologs in various genomes were adjacent to various ribosomal proteins on the chromosome. Conclusion: The human genome contains at least two processed pseudogenes of PDCD5 . Besides the relationship with cell apoptosis, PDCD5 is predicted to have functional relationship with ubiquitin and participate in the translation regulation.

In recent years,more and more Macrolide resistance of Mycoplasma pneumoniae becomes a hot research.The current studies suggest that the mechanisms of Macrolide resistance are drug-binding site mutations,ribosome methylation,drug efflux,and drug related fire-fighting,in which the drug target 23srRNA gene 2063,2064 mutation is significant.It has been reported that drug-resistant patients have the poor effect,including the long total febrile days and high dressing.For treatment of drug-resistant patients,most studies still use the macrolide,early use of corticosteroids may be effective.It is uncertain whether the increase in resistant strains is related with the use of macrolide.It is significant to carry out future researches in this area for clinical therapy and put off the prevalence of drug-resistant strains.

With the standardized training and spreading,laparoscopic total mesorectal excision for rectal cancer has been spread nationwide.The oncological adequacy and long-term outcomes have also been confirmed by series of randomized controlled trials such as COLOR Ⅱ in Europe.There were still some technical issues,such as safe surgical plane,surgical approach,lymph node dissection,and function-preserving,which were still under debate.In the current article,authors will discuss the issues with clinical experiences about preserving of Denonvilliers facial,preserving of pelvic autonomic nerve,strategy of surgical approach,dissection of lateral lymph nodes,and necessity of protective diverting stoma.

Objective To investigate the effects of substance P (SP) pretreatment on the expression of β1-adrenoreceptor(β1-R) induced by norepinephrine (NE) in cultured neonatal rat cardiomyecytes.Methods The cardiomyocytes obtained from 1-3 day old SD rats were cultured for 72 h.The experiment was performed in 2 parts.In part 1 the cells were seeded in 15 well plate and randomly divided into 5 groups (n=3 wells each):control group (C1)and 4 NE groups were exposed to NE 10-9, 10-8,10-7,10-6 mol/L respectively (NE1,2,3,4).In part Ⅱ the.cells were seeded in 12 well plate and randomly divided into 4 groups (n=3 wells each): group I control (C2): group II NE 10-7 tool/L; group Ⅲ (SN)was pretreated with SP 10-6 mol/L 30 minbefore NE 10-7 mol/L and group IV (NSN)was treated with NK-1 receptor antagonist (NK-1 tachykinin receptor antagonist,S3144) 30 min before SP pretreatment.After exposure to NE for 3 h the expression of β1-R in the rat cardiomyecytes was detected using flow cytometry.Results In part Ⅰ the expression of β1-R was significantly higher in group NE1-3 than in control group (C1),with the highest expression in group NF3.In part Ⅱ the expression of β1-R was significantly higher in group Ⅱ (NE) than in control group (C2) while lower in group Ⅲ (SN) than in control group Ⅱ.(NE).There was no significant difference in the expression of β1-R between group Ⅰ (C2) and group Ⅲ and Ⅳ (NSN).Conclusion Substance P pretreatment can inhibit the up-regulation of β1-R expression in cultured rat cardiomyocytes induced by norepinephrine through activating NK-1 receptor.