Objective To investigate the correlation between abnormal lipid metabolism and coronary artery lesions. Methods Two hundred and sixty seven patients were devided into the control group ( n =101) and the coronary heart disease (CHD) group ( n =166), which included single vessel lesion ( n =54), double vessel lesions ( n =68) and triple vessel lesions ( n =44) according to coronary angiography. At the same time the lipid components were examined and the ralationship between serum lipid level and coronary artery lesions were analysed. Results Compared with the control, the total cholesterol (TC), low density lipoprotein cholesterol (LDL-C) and triglyceride (TG) level were significantly elevated while but high density lipoprotein cholesterol (HDL-C) was lowered in the CHD group. The severity of coronary lesions was increased with the elevation of TC, LDL-C, apolipoproteinB (ApoB), TC/HDL-C, LDL-C/HDL-C, and the reduction of HDL-C. There were significant differences in terms of lipid level beteewn the CHD group and the control. The analysis showed that TC/HDL-C and LDL-C/HDL-C were most directly correlated with the coronary artery lesions in the CHD group. Conclusion The results suggest that the severity of coronary artery lesions correlated with abnormal lipid metabolism, and LDL-C/HDL-C, TC/HDL-C are better indicators than other lipid components.

Oridonin is ent-kaurane diterpenoid and is one of the efficacious component of Rabdosia Rubesecens ( Hemel. ) Hara in Henan province. In this experiment, the effects of Oridonin on hemody-namics of anaesthetized rabbits were studied. Hemodynamics effects of Oridonin (3.0 mg, 10.0mg/kg iv)were noticed. LVSP, ?dP/dt_max, Vpm, Lo, HR, SBP, DBF were decreased. t-dP/dt_max was increased. LVEDP did not changed. Oridonin decreased DBP more than SBP, it is suggested that Oridonin. may reduce vascular smooth muscle tone.

Objective To investigate the effect of comprehensive rehabilitation on the stroke.Methods 120 cases of stroke divided into rehabilitation group(60 cases)and control group(60 cases).They were assessed with Fugl-Meyer Assessment(FMA),Modified Barthel Index(MBI),and Satisfaction with Life Scale(SWLS)before and 1 month,3 months and 6 months after treatment respectively.Results The scores of FMA,MBI and SWLS were improved significantly in rehabilitation group,especially in the 1st month,while that of control group presented the tendency of decline.Conclusion Comprehensive rehabilitation is effective on motion function,activity of daily living and quality of life of stroke patients.

Except common histologic type,some special histological types and clinico-pathological features of gastric cancer,such as neuroendocrine carcinoma,hepatoid adenocarcinoma,lymphoepithelioma-like gastric carcinoma and hereditary diffuse gastric cancer,etc,because of there special diagnosis and treatment measures and prognosis,should be taken into account in our clinical work.This article reviews the clinical progress of diagnosis,treatment and prognosis of the four special kinds of gastric cancer mentioned above.

This article analyzed the types and causes of clinical research misconduct including the definition and background.We then set up the research management system to control and prevent such misconduct behavior.Our strategy includes using various technical tools to actively carry out clinical research integrity related education and training program,establishing integrated control model,and clinical research misconduct handling mechanism to jointly cope with the problems,to promote a good atmosphere for clinical research and to establish the integrity of the research.Our goal is to promote the medical technology development and to improve the patient care.

BACKGROUND:Though the effects of conservative or traditional open reduction and internal fixation in the treatment of thoracolumbar fractures are reliable and satisfactory for most cases, two methods also have shortages. Minimal-invasive percutaneous pedicle screw system provides a new available method. OBJECTIVE:To investigate the clinical effect of Sextant percutaneous pedicle screw system in the treatment of thoracolumbar fractures. METHODS:A total of 55 patients, who had undergone percutaneous pedicle screw fixation using Sextant system (25 patients) or traditional open internal fixation (30 patients) for single-level vertebral body compression fractures in Department of Orthopedics of Renmin Hospital of Wuhan University from February 2011 to January 2013, were enrol ed in this study. RESULTS AND CONCLUSION:Except two patients in traditional open internal fixation group were lost after discharge, al other patients were fol owed up for 8-14 months. Operative time, intraoperative blood loss, postoperative drainage amount and hospital day were better in percutaneous pedicle screw fixation group than in the traditional open internal fixation group (P<0.05). Compared with preoperation, the sagittal Cobb angle, visual analogue scale and Oswestry disability index after operation were significantly lower (P<0.05), while anterior vertebral body height ratio was significantly higher in each group (P<0.05). No significant difference was found in terms of correction loss and Oswestry disability index between two groups at 8 months after operation (P>0.05). The results show that percutaneous pedicle screw fixation using Sextant system has a satisfactory outcome in the treatment of thoracolumbar fractures. However, obeying indication strictly is very important for clinical application.

Objective The aim of this study was to observe the growth difference and expression of cytochrome C of skin hair follicles in neonatal mice .Methods The morphology of different skin hair follicles of neonatal mice ( postnatal day 1-9)were observed by HE staining histology and cytochrome C was detected by immunohistochemistry .Results The skin hair follicles in different parts of neonatal mice showed differences not only in morphology but also in developmental pe -riods.Hair follicle growth in the back and tail skin had a nonlinear and growing period .After the nonlinear and growing pe-riod they began to grow rapidly .The tail development was slightly slower than that on the back .The hair follicles of vibris-sae were very special , and started to develop without a stable period .Conclusions The results of morphological observa-tion and cytochrome C immunohistochemistry demonstrate that differences exist in the hair follicle morphology and develop -mental times in the skin of different parts of the body in neonatal mice .

Objective To investigate the effects of caveolin-1 overexpressing on the growth of cervical squamous cell cancer Hela cell line. Methods Eukaryotic expression vector of human caveolin-1 gene was introduced into Hela cells by Lipofectamine. The clones stably overexpressed caveolin-1 were identiffed by RT-PCR, immunofluorescence cell staining techniques and Westernblotting. Cells proliferation viabihty was tested by MTT assay, and flow cytometry was used to assay the cell cycle and apoptosis, and the relative phosphorylation level of extracellular regulated protein kinases (Erk1/2) were detected by Westernblotting. Results The clones stably overexpressed caveolin-1 were obtained. Compared with the parental Hela cells, the tranfected cells exhibited a slower rate of growth. FAGS analysis results revealed that overexpression of caveolin-1 resulted in the cell cycle arrest in the G0/G1 [ ( 68. 04 ± 2. 57 ) % vs ( 53.41 ±1.01)%] phase and increased the apoptotic cell fraction[ (19. 18 ±2.20)% vs (5.63 ±0.55)%, P <0. 05 ]. Western blotting results showed that overexpression of caveolin-1 reduced the phosphorylation of Erk1/2(0.28 ±0.05 vs 0.81 ±0.07, P <0.05). Conclusions Overexpression of caveolin-1 suppressed the growth of Hela cells and induced apoptosis, down-regulation of Erk1/2 phosphorylation might be involved in its mechanism.

BACKGROUND:The effects of both conservative and traditional open reduction and internal fixation are dissatisfactory in the treatment of thoracolumbar fractures, however, minimal-invasive percutaneous pedicle screw-rod systems provide a new available method.
OBJECTIVE:To investigate the clinical effect of percutaneous pedicle screw fixation using Sextant minimal-invasive system in the treatment of thoracolumbar fractures.
METHODS:A total of 55 patients, who had undergone percutaneous pedicle screw fixation using Sextant system (25 patients) or open pedicle screw fixation (30 patients) for single-level vertebral body compression fractures in Department of Orthopedics, Renmin Hospital of Wuhan University from February 2011 to January 2013, were enrol ed in this study. They showed no neurological signs and symptoms. The operative time, intraoperablood loss, postoperative drainage amount, length of hospital stay, pre-and post-operative Cobb angle and anterior vertebral body height ratio were recorded and compared between two groups.
RESULTS AND CONCLUSION:Except two patients in open pedicle screw fixation group were lost after discharge, al other patients were fol owed up for 8-14 months. There were significant differences in operative time, intraoperative blood loss, postoperative drainage amount and length of hospital stay between two groups (P=0.000 0). The post-operative Cobb angle was significantly lower while anterior vertebral body height ratio was significantly higher at one week than those before fixation in two groups (P=0.000 0). No significant difference was found in correction loss between two groups at 8 months after operation. Experimental findings indicate that, percutaneous pedicle screw fixation using Sextant system has a satisfactory outcome in the treatment of thoracolumbar fractures. However, understanding the correct indications is very important for clinical application.

Objective To investigate the status quo of nutrition knowledge,attitude and practice(KAP) among elite female small ball athletes of Guangdong province and its influencing factors.Their serum ferritin was also observed to explore its correlation with their KAP,so as to provide evidences for formulating and implementing scientific and reasonable nutrition education schemes.Methods Fifty-four female small ball athletes,including 20 handball,20 hockey and 14 softball players,were selected to complete a self-designed diet questionnaire based on KAP education mode and their serum ferritin indicators were analyzed at the same time.Results (1)The average scores(all out of 100)of nutrition knowledge (K),attitude (A) and practice (P) were 43.64 ± 11.66 (with a failure rate of 90.7％),78.37 ± 10.21 (with the rate of excellence of 59.3％)and 63.10 ± 10.17(with a passing rate of 37.0％),and the average KAP score was 185.10 ± 23.01,with a passing rate of 44.4％.(2)The one-way ANOVA found that there were significant differences in the average A score and the total KAP score (P＜0.05) among the athletes of different small ball teams.The correlation analysis results showed that the average A score was of significant positive correlation to the average P score.Moreover,the average KAP total score was of significant correlation with sports events (P＜0.05) and the education level (P＜0.05),but not with the age,training period and exercise level(P＞0.05).The multiple stepwise regression analysis results showed that training period(P＜0.05) and sports events(P＜0.05) was the influencing factor of nutrition knowledge and attitude respectively,and that education level (P＜0.01) was the influencing factor of both nutrition practice and KAP.(3)Significant differences were found in the serum ferritin among athletes of different sports events(P＜0.05)the serum ferritin was of significant positive correlation with the nutrition knowledge(P＜0.05).Conclusion (1)The lack of sports nutrition knowledge and the unbalance of nutrition behavior are common in elite female small ball athletes,but they had a positive attitude.It is necessary to carry out a series of effective nutrition education programs to improve their sports nutrition knowledge,cultivate their good nutrition attitudes and finally formulate rational and effective nutritional intervention measures to promote the formation of healthy nutrition behavior of athletes.(2) The sport event is the key influencing factor of the nutrition attitude and dietary behaviors,which suggests that more more attention should be paid to athletes' dietary management and focus should be put on their diary behavior on no-training days and on their energy ratios control of each meal,especially breakfast and extra meals,and rational intake of snacks.(3)The incidence of iron deficiency is consistent with KAP questionnaire survey results,which indicates that it may be an effective measure to prevent iron deficiency anemia through strengthening athletes' dietary nutrition education.