1.Application of two fall assessment scales on the elderly patients
Honglu DUAN ; Mei WANG ; Huamei ZHENG
Modern Clinical Nursing 2013;(11):26-28
Objective To reduce the feasibility of combining the two fall assessment scales in nursing patients..Methods Two nurses were assigned to conduct the assessments among 60 senile patients using Morse assessment scale and Hendrich Ⅱassessment scale to screen patients with high-risk fall.Result The number of high-risk patients using Morse assessment scale was larger than that using Hendrich II assessment scale(P<0?05) Conclusions The combined use of Morse fall assessment scale and HendrichⅡfall assessment scale may make up the shortcomings of each other,assess the risk factors and predict the high risk factors.
2.Auricle fibrosarcoma:a case report.
Jian MA ; Mei ZHENG ; Gangping WANG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2015;29(1):93-94
A male patient, 67 years old, was admitted with the left auricle neoplasm over two months, increased rapidly for 10 days, on November 5, 2013. The tumer is about 2. 0 cm ×× 2. 0 cm size, smooth surface, no burst, hard, painless, and immobilization. After admission biopsy, the pathological report: "spindle cell sarcoma", thin a total resection of the left auricle under local anesthesia was made, postoperative pathological report: tumor by short of spindle cells, arranged in bunchiness, a small number of seats is arrangeA striate;The nuclei are hyperchromatic and part of the visible nucleoli and empty bright cytoplasm, pathological nuclear fission was visible. "Scar" on the matrix of collagen, hardening, and change the glass samples. The tumor destruction of cartilage. immunohistochemical stainin : Vimentin (+), CD99 (-), Bcl-2 (-), CD34 (-), SMA (-), Des (-), HMB45 (-), MelanA(-), S-100(-), CK(-). Diagnosis as fibrosarcoma. The patient refused any radiation or chemotherapy. Postoperative follow-up of 8 months, no local recurrence and distant metastasis.
Aged
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Ear Neoplasms
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diagnosis
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Fibrosarcoma
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diagnosis
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Humans
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Male
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Neoplasm Recurrence, Local
3.The effects of calcium dobesilate combined with irbesartan in treating early diabetic nephropathy
Mei PENG ; Xiancong WANG ; Kai LUO ; Qinghua ZHENG ; Huanlan WANG
Chinese Journal of Primary Medicine and Pharmacy 2012;19(16):2434-2435
ObjectiveTo observe the therapeutic effects of calcium dobesilate combined with irbesartan in treating early diabetic nephropathy ( DN ).Methods65 patients with early DN were randomly divided into two groups,experiment group( irbesartan plus calcium dobesilate,n =34) and control group( irbesartan,n =31 ).After 24 week treatment,the urinary albumin excretion rate(UAER),fasting blood glucose ( FBG ),postprandial blood glucose (PBG) and glycosylated hemoglobin(HbA1c) were detected and compared between two groups before and after treat ment.ResultsUAER after treatment was significantly reduced in both 2 groups(t =8.074,12.89,all P<0.01),and the decrement in the experiment group was more obvious than that in the control group with statistical significance ( t =3.965,P < 0.05).ConclusionCalcium dobesilate combined with irbesartan has good effects in the treatment of early DN.
4.Scalp psoriasis in China: a survey on epidemiological characteristics and influencing factors
Mei JU ; Gang WANG ; Min ZHENG ; Hongzhong JIN ; Heng GU
Chinese Journal of Dermatology 2014;47(8):533-537
Objective To describe epidemiological characteristics of scalp psoriasis in China,and to determine its influencing factors through a hospital-based,multicenter and large-scale survey.Methods An epidemiological survey was conducted on patients with scalp psoriasis in 42 hospitals in 11 provinces and 4 municipalities.A questionnaire was designed to collect general and clinical information of patients,including clinical type,lesion distribution,psoriasis scalp severity index (PSSI),aggravating factors,influence on quality of life,treatment course,degree of satisfaction with treatment,etc.Participants were asked to fill out the questionnaire with the help of well-trained dermatologists.Data were processed with the EpiData software.The WPS system and Epi Info 5.0 statistics software were used for descriptive analysis of the data.Results Totally,3 901 patients participated this study,and 3 586 valid questionaires were obtained.The mean age at onset of scalp lesions was (27.40 ± 13.34) years,and the male to female ratio was 1.6∶1 with a mean PSSI score of 13.61 ± 12.31.The hairline of the forehead was the most frequently affected area with the constituent ratio of forehead psoriasis being 74.3% (2 663/3 586).The duration of scalp psoriasis reached 3 to 6 months every year in 1 136 (31.7%) patients,and the area affected by scalp psoriasis was less than 25% of the total surface area of the scalp in 1 649 (46%) patients.The most frequent clinical presentation was scaling (92.1%),followed by erythema (80.6%),itching(76.7%),bunchy hairs (39.9%) and hair loss(18.0%).Disease aggravation was most frequently associated with seasons (76.7%),emotions (47.4%),stress (47.1%) and fatigue (46.6%).The quality of life (especially social,outdoor and entertainment activities) was reported to be negatively influenced by this entity to different degrees in 2 529 (70.5%) patients.Of these patients,583 (17.8%) received combination therapy (topical and systemic),with glucocorticoid ointment (27.5%),calcipotriol scalp solution (22.7%) and glucocorticoid-containing solution (11.9%) being the most commonly used topical drugs; only 38.6% complied with medication requirements and doctors' advice for 3 to 5 weeks,and 35.2% believed that the primary reason for poor compliance was unsatisfactory response to drugs.It was considered that an ideal drug for the treatment of scalp psoriasis should be highly effective (50.3%),safe (17.8%) and easy to apply (11%) with a rapid onset of action(11.9%).Conclusions Scalp psoriasis has a predilection for the hairline of the forehead with scaling as the main clinical presentation.Winter and spring seasons appear to be the most common aggravating factor of scalp psoriasis.The quality of life is negatively influenced to different degrees in many patients with scalp psoriasis.Topical corticosteroids plus non-hormonal drugs should be the first choice for the treatment of scalp psoriasis.Patients consider that the ideal drugs for the treatment of scalp psoriasis should be effective,safe,and easy to apply.
5.Generation of Apak stably knockout cell lines and variation of their p53 activity and apoptosis
Xingsha MEI ; Jian WANG ; Huizhen ZHENG ; Chunyan TIAN
Military Medical Sciences 2016;40(4):299-303
Objective To establish an Apak gene stable and permanent knockout cell line using CRISPR/Cas9 system in human colon cancer cells ( HCT116 cells), and study the effect of Apak knock-out on p53 activity and apoptosis. Methods The lentiCRISPR v2-sgRNA Apak expression plasmid was co-transfected with lentivirus coated plasmids pSPAX2 and pMD2.G.The supernatant was collected, filtered, and used to infect HCT116 cells.The positive clones were screened out by puromycin culture and Western blot was used to detect Apak knockout cell lines.Luciferase reporter gene assay, flow cytometry analysis and colony formation assay were used to examine p53 activity and apoptosis of Apak knockout cells, respectively.Results Apak knockout HCT116 cell lines were generated in which p53 activity and apoptosis were increased,but the colony formation was decreased.Conclusion The Apak stable knockout cell lines of HCT116 are successfully generated by CRISPR/Cas9 system for further functional study.
7.Experimental observation of electrical stimulation on cultured nervous system cells
Zheng-mei WANG ; Jian YANG ; Rong-hua LU ; Yongqi XU
Chinese Journal of Rehabilitation Theory and Practice 2002;8(11):664-665
ObjectiveTo explore the influence of electrical stimulation on nervous cells.MethodsOn 5th day the brain primitive nervous cells of rat brain, in vitro culturing, were divided into 4 groups, in order of control group, electrical pole control group, low stimulation group, and high stimulation group. The cells were stimulated with electro-acupuncture apparatus for 7 days, 30 minutes once, twice a day. On 10th day, the cells were observed microscopically for 3 days and taken photos.ResultsElectrical stimulation is helpful to raise the survival rate of cell.Conclusions Electrical stimulation influences morphological change of nervous cells during culturing.
8.Relationship between Leu72Met polymorphism of Preproghrelin gene and type 2 diabetes mellitus and diabetic nephropathy
jia-mei, JIANG ; yong-ning, SUN ; li-mei, LIU ; tai-shan, ZHENG ; nian-song, WANG ; feng, WANG
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(07):-
0.05). Conclusion Preproghrelin-Leu72Met is not significantly associated with T2DM and DN in Shanghai Han populations,while T2DM with AA genotype is characterized by significant declination in urine microalbumin when compared with CA and CC genotypes.Leu72Met polymorphism(C→A)may postpone the development of microalbuminuria in T2DM subjects.
9.Clinical Research of Color Doppler Ultrasound Assign Points Diagnosis for Triple-Negative Breast Cancer and Axillary Lymph Node Metastasis
Yi ZHANG ; Ying ZHANG ; Mei YUAN ; Shufang ZHENG ; Wei WANG ; Yali ZHOU ; Wenxiao LI ; Haibo WANG
Progress in Modern Biomedicine 2017;17(26):5066-5073
Objective:To explore clinical application value of color Doppler ultrasound (CDU) assigned to diagnose triple-negative breast cancer (TNBC) and axillary lymph node metastasis (ALNM).Methods:A retrospectively analyzed of proved of clinical data on the 863 patients breast cancer in women by CDU inspection,operation and pathology.According results will be divided into TNBC group and non negative breast cancer (NTNBC) group via clinical pathological and immunohistochemical detection.Using multi-factor unconditioned Logistic regression analysis the CDU supposed the diagnosis and the correlation of TNBC.Results:The coincidence rate of CDU and pathological diagnosis was 90.96% (785/863),78 cases (9.04%) remove breast masses,105 patients TNBC group and NTNBC group 680 patients.The mass circular or elliptic (48.57% vsl0.70 %),the tumor maximum diameter 5.0 cm (21.90% vs 15.30 %),micro calcifications (87.62% vs 74.12 %),state clearly (56.19% vs 41.47 %),no burr edge character (74.29% vs 41.62 %),peripheral hyperechoic halo ring (74.29% vs46.76 %),rear echo attenuation (72.38% vs 46.76 %),no ALNM (61.90% vs 37.35%) comparing the two groups exists significant difference (P< 0.05),while the mass number,and vertical and horizontal diameter ratio,degree of echo,echo distribution and blood flow classification comparing the two groups were no significant difference (P> 0.05).The CDU fu points > 10 divided into NTNBC and pathological coincidence rate 93.93% (680/724),8 ~ 10 points TNBC suspected diagnosis coincidence rate 11.51% (16/139),< 8 points TNBC tendency diagnosis coincidence rate 64.03% (89/139),TNBC total diagnostic coincidence rate was 75.54% (105/139).In CDU diagnosed 785 cases of breast cancer,the ALN enlargement in 319 cases (40.64%),with TNBC group was 61.90% (65/105),NTNBC group was 37.35% (254/680).Logistic regression analysis results:tumor assumes the circular or elliptic,edge without burr,peripheral hyperechoic halo ring,rear echo attenuation and ALNM with TNBC has certain correlation (P<0.05).Conclusion:The seemingly benign breast diseases and axillary lymph node enlargement,the CDU,supposed less than 8 points,age 50 or less or not at the same time,menopause or having a history of breast cancer susceptibility to family,important reference index,clinical value has high of CDU diagnostic of TNBC and ALNM.
10.Alteration of Plasma Neuropeptide Y in Patients with Essential Hypertension
Rumin ZHANG ; Mei GAO ; Zheng ZHANG ; Shifu WANG ; Li JIANG ; Huaiquan WANG ; Bin ZHU
Chinese Journal of Hypertension 2006;0(09):-
Objective To observe the change of plasma neuropeptide Y(NPY) concentration in patients with essential hypertension,and its relevance to target organ damage.Methods The plasma NPY concentration was determined by radioimmunoassay(RIA) in 115 patients with essential hypertension,including 45 patients with single target damage(left ventricle hypertrophy 21,stroke 13,nephropathy 11) and 19 patients with multiple target damage,with 30 normal as controls.Results 1) Plasma NPY concentration in patients with essential hypertension was significantly higher than normal controls(P0.05).Conclusion NPY may involve in the physiopathologic progress of essential hypertension and associate with the development of target organ damage.Evaluation of plasma NPY concentration may be valuable biomarker in estimation the severity of hypertension and target organ damage.