Objective:To study the antibacterial activity of dental amalgam against S.mutans in vivo.Methods:Plaque samples were collected from amalgam surface 1 d,1,2,4 and 8 weeks after tooth restoration with amalgam( group of fresh amalgam) in 16 subjects and from anothor 14 subjects with amalgam tooth restoration for more than 3 years (group of aged amalgam).Plaque samples were collected only once.Isolation frequency (IF) and colony forming unite(CFU) of S.mutans were tested.The plague samples from oposite tooth were used as the controls.Results:In the group of fresh amalgam IF and CFU of S.mutans were heigher on amalgam than on the control teeth(P0.05).Conclusion:Amalgams may have inhibitory effects on the growth of S.mutans.

Cardiomyopathy, Hypertrophic ; Humans ; Infant, Newborn ; Infant, Premature

Training of doctor receiving in-service training is the important part of clinical education in teaching hospital. It will improve the clinical and scientific research ability of the doctor receiving in-service training and will train high-quality medical persons for primary hospital by training before going to post,clinical teaching,surgical operation teaching,standardized special lecture,case report and various kinds of science activities.

Objective:To investigate effects of strengthening the spleen and tonifying the kidney and their combination before and after exercise on relative indexes of metabolism of glucose and amino acids.Methods:Strengthening the spleen alone,tonifying the kidney alone or their combination before and after exercise was given to the rat receiving 7-week gradually increasing load exercise on a running platform,and after quantitative load,contents of serum lactic acid,urea nitrogen,glucose,hepatic glycogen,muscle glycogen,hemoglobin,and malondialdehyde(MDA)in renal tissue and activity of superoxide dismulase(SOD)in the renal tissue were determined.Results:Strengthening the spleen before exercise increased hepatic and muscle glycogen contents,and tonifying the kidney before exercise increased muscle glycogen content,and strengthening the spleen combined with tonifying the kidney before and after exercise increased hemoglobin level,strengthening the spleen followed by tonifying the kidney before exercise decreased serum lactic acid and urea nitrogen levels,increased muscle glycogen content and SOD activity/MDA content ratio in the kidney.Conclusion:Strengthening the spleen and tonifying the kidney play a certain role in reducing motion-related fatigue,while strengthening the spleen followed by tonifying the kidney before exercise has the best results.

Recombinant antibodies have become the major growth trends in biotech industry following their success on therapeutic application and good revenue. But the low level of mammalian expression and laggard fermentation process constrained the development of antibody industry in China. The global advances of antibody industry were reviewed, compared the respective advantage between dihydrofolate reductase and glutamine synthetase expression system, continuous perfusion and fed-batch processes were compared. Finally, based on the knowledge and experience of antibody expression and fermentation, the suitable strategy of antibody industrialization, e.g. the fermentation model and scale, should depend on the comprehensive consideration of entrepreneur for the productivity, manufacturing capacity and market revenue. It may be a wise choice to use glutamine synthetase expression system and continuous perfusion process for the need of Chinese antibody industrialization.

Objective To present a retrospective analysis of the clinical outcome in patients treated by local intra-arterial thrombolysis (LITT). Using urokinase for acute ischemic stroke of the anterior or posterior arterial circulation.Methods Twenty-six patients were treated with LITT using urokinase. The effects all patients were assessed by using angiography, brain CT and stroke scale before and after treatment. Results Among 26 patients, the complete reperfusion of blood vessel appeared in 7 and partial reperfusion in 11.The efficiency rate reached 78%.Conclusions Cerebral arterial thrombolysis with UK can improve neurologic function defect in 84% of patients with acute ischemic stroke after 15 days, suggesting one of the best ways for treatment.

Objective To investigate the clinical efficacy and significance of preoperative superselective endovascular embolization in treatment of patients with vascularized meningioma.Methods 3-9 days before operation, 98 patients with vascularized meningioma underwent DSA and preoperative superselective embilization with PVA particles, gelatin sponge, and silk. The surgical intervention was performed after embolization. Results The vascularized meningioma was mainly supplied by the middle meningeal artery, ascending pharyngeal artery, occipital artery, internal maxillary artery as well as submeningeal artery. After the supplying artery was embolized, tumors' staining in 42 cases disappeared completely and that of 56 cases disappeared totally or partly. Most patients were operated on 3-9 days after embolization. 64 tumors were removed completely, while other 34 cases were removed totally or partly. The average volume of bleeding during the operation was 950 ml in the former and 1 500 ml in the latter.Conclusions The best time for operation is 7～9 days after embolization. Preoperative embolization of the vascularized meningioma contributes to reduce the bleeding significantly during the operation, increase the safety of the operation and improve the total removal of the tumor. It is a safe and effective microinvasive method.

Objective To appraise the values of combined determination of cTnI,MYO and CK-MB mass in the diagnosis of viral myocarditis.Methods The myocardial injury markers(cTnI,MYO and CK-MB mass)and myocardial enzyme(LDH,AST,CK and ?-HBDH)of 61 patients with viral myocarditis(VMC),74 patients with non-viral myocarditis(NVMC)and 46 healthy subjects were determined,respectively,by microparticle chemiluminescent immunoassay and continuous monitoring assay.Diagnostic efficiency was calculated by matrix decision method.Results The serum levels of cTnI,MYO and CK-MB mass of the patients in VMC group were obviously higher than that of the patients in NVMC group(0.46?0.21 vs 0.06?0.04?g/L,98.7?38.2 vs 39.2?26.8?g/L and 6.1?4.2 vs 2.2?1.7?g/L,respectively)as well as that of healthy subjects(0.46?0.21 vs 0.07?0.04?g/L,98.7?38.2 vs 36.5?24.7?g/L and 6.1?4.2 vs 2.1?1.5?g/L,respectively,P0.05).For preliminary diagnosis of VMC,the SE of MYO is highest,but the SP of cTnI and CK-MB mass were better choice.Restoration of MYO was earliest while cTnI was latest during convalescence of the patients.Conclusion Combined and uninterrupted determination of the myocardial injury markers can provide better diagnostic accuracy.

20 mm),these three imaging techniques(HAP-MDCT,Lipiodol CT and DSA)had the same sensitivity,for 10~20 mm hepatic nodules,the detecting rate was no significant difference among them,while,for

Objective:To compare the healing of gingiva after electrosurgical gingivectomy with different power outputs and electrodes. Methods:15 rabbits were randomly assigned to 3 groups which used different electrodes: needle electrode, long loop electrode and loop electrode. 1 animal was chosen as control in each group. Free gingiva of anterior teeth had been removed 2 mm in height with different power output and electrosurgical knife. Gingival specimens were harvested for histopathological study on days 1,7,14,and 21 after operation. Statistical analysis was performed on wound healing. Results:Electrical knife with medium power output caused significantly higher degree of inflammation than other techniques(P0.05). Conclusion:When electrosurgery is used on gingiva, there is no significantly difference with different electrodes. Longer observation is needed after gingival electrosurgery with medium power output electrical knife.