Background Studies showed that traumatic optic neuropathy (TON) can activate and induce immuno-inflammatory response,while T helper cell 17 (Th17), a CD4+ T lymphocyte, is associated with immunoinflammatory response.However,the effects of Th17 on the development of TON are unclear.Objective This study was to observe the changes of Th17 lymphocytes in spleen and the expression of interleukin-17 (IL-7) in retina in TON rats.Methods Seventy male 4-week-old SD rats were randomly divided into normal control group, sham operation group and TON 1-, 3-,7-, 14-and 28-day group according to random number table.The optical nerves of the right eyes were exposed and impacted at a 25° angle by using fluid percussion injury (FPI) device with the average force (699.1±60.8) kPa to create the TON models in the TON groups,and only optical nerves were exposed in the sham operation group.Flash visual evoked potential (F-VEP) was recorded and the amplitude and latency were measured to evaluate the function of optical nerves of the rats.The rat splenocyte suspension in various groups was prepared for the detection of Th17 cells by flow cytometry, and the expression of IL-17 protein was detected by immunochemistry.Results No significant differences were found in the amplitudes and latencies of P2 wave, proportion of Th17 cells and the IL-17 positive cell numbers in retina between the normal control group and sham operation group (P =0.829,0.830,0.856,0.496).The amplitudes of P2 wave were significantly lower, and the latencies of P2 wave were significantly longer in the TON groups than those in the sham operation group (all at P＜ 0.05).The proportions of Th17 positive CD4+ T lymphocytes were (0.94±0.13)％,(1.80±0.18)％,(1.98± 0.20)％ ,(2.34±0.20)％ ,(2.11±0.13)％ ,(1.92±0.18)％ in the sham operation group,TON 1-day group,TON 3-day group,TON 7-day group,TON 14-day group and TON 28-day group, respectively, showing significant differences between the sham operation group and various TON groups (all at P＜0.05).In addition, the IL-17 positive cell numbers in retina were 796.326 ± 100.028, 1 673.416 ± 188.021,1 892.431 ± 151.026,2 420.454 ± 256.024,1 996.429±177.022 and 1 629.410±127.023 in the sham operation group,TON 1-day group,TON 3-day group,TON 7-day group,TON 14-day group and TON 28-day group, respectively, showing significant differences between the sham operation group and various TON groups (all at P＜0.05).Conclusions In rats of TON, the proportion of Th17 cell in splenocytes and the expressions of IL-17 in retina are increased,suggesting that Th17 and IL-17 participate in the progression of TON.

Background Keratectasia after laser in situ keratomileusis (LASIK) is one of the serious complications which affect the vision.The measurement of cornea posterior elevation is an important way helping to find this change.Objective The aim of this study was to investigate the change of cornea posterior elevation after LASIK in myopic eyes with astigmatism and affecting factors.Methods A series case-observational study was adopted.One hundred and twenty-seven myopic eyes with astigmatism of 66 patients who received LASIK in 2008 May through 2010 January in Beijing Tongren Hospital were retrospectively analyzed to evaluate the change of cornea posterior elevation following the LASIK.The parameters related to cornea posterior elevation were measured and compared before and 3 months,6 months and 1 year after surgery,respectively,with Oculyzer anterial segment analysis system.Results The height values from posterior cornea highest point,the lowest point and central vertex central elevation zone were (12.20±3.39),(-19.02±7.38) and (1.05 ±3.25) μm respectively before LASIK and were (14.38±3.80),(-18.55±7.11),(2.83±4.81)μm in3 months and (13.99±3.38),(-17.57±6.54),(2.45±4.61) μm in 6 months after LASIK.They were (14.40±3.85),(-17.76±6.00),(2.16±5.00) μm in 1 year after surgery.Significant increases were found in the vertex height and central elevation after LASIK compared with before surgery(highest pointq=6.813,5.594,6.875,all at P＜0.001.central vertex:q=4.488,P=0.002;q =3.530,P=0.013 ;q =2.799,P =0.047).However,no significant difference was seen in various time points after LASIK (P＞0.05).A positive correlation was obtained between the height value of the posterior cornea central vertex with the spherical equivalent (SE),maximum cutting depth or cutting proportion 1 year after LASIK (r =0.295,0.297,0.295,all at P=0.001),and there was a negative correlation between it with residual stroma (r=-0.208,P=0.019).The intraocular pressure (IOP) measured with noncontact tonometry (NCT) was (14.24±3.33) mmHg before LASIK,and those of 3 months,6 months and 1 year were (8.42± 1.90),(8.61 ± 1.64) and (8.76± 1.64)mmHg after LASIK,showing a significant lowing in IOP after LASIK in comparison with before LASIK (q =29.851,28.317,26.337,all at P＜0.001).But no significant change was found in the different time points after LASIK (P＞0.05).There was significant difference in the IOP corrected by Ehlers after LASIK (P＞0.05).The IOP before LASIK had positive correlation with the central elevation of posterior cornea surface 1 year after LASIK(r=0.258,P =0.003).Conclusions The cornea posterior elevation increases slightly early stage after LASIK but retains stable level with lapse of time.The refraction diopter before LASIK is the main factor affecting the posterior corneal shape.To reduce the risk of corneal ectasia,more attention should be paid to the reserving of appropriate corneal residual stroma and smallest degree of eccentricity during the ablation procedure.

Acupuncture Therapy ; Animals ; Female ; Humans ; Polycystic Ovary Syndrome ; therapy

OBJECTIVETo dynamically assess drug targeting of Yougui Pill (YP) and Zuogui Pill (ZP) using infrared thermography.

METHODSIn this self-control experiment, five healthy volunteers were recruited. By using infrared thermography 10 to 11 thermal images of different body locations were taken from each participant after they took warm water, YP, ZP, and their dissembled prescriptions at 30, 70, 100, 130, and 160 min, respectively. The heat values in the lower quadrant abdomen, uterus, Du channel, and Shenque (CV8) were statistically analyzed after scanning for 125 times.

RESULTSAdministration of YP and its disassembled prescriptions enhanced the heat value of the locations of the Du channel and Shenque (CV8), but did no enhance the heat value of the lower quadrant abdomen at 30 min. Administration of ZP and its disassembled prescriptions reduced the heat value in the locations of the lower quadrant abdomen, uterus, Du channel, and Shenque (CV8) at each time point.

CONCLUSIONThe drug targeting of ZP and YP focused on the locations of the Du channel and Shenque (CV8), not on the locations of the lower quadrant abdomen or uterus.

Adult ; Drug Delivery Systems ; Drugs, Chinese Herbal ; administration & dosage ; Female ; Humans ; Infrared Rays ; Thermography ; methods ; Young Adult

Objective To investigate characteristic skin lesions and typical histopathological changes of adult-onset Still's disease(AOSD) for its early diagnosis and treatment.Methods Clinical data were collected from 8 patients with AOSD,and analyzed retrospectively.Results All the patients had transient rashes and persistent papules/plaques during the course of disease.Of the 8 patients,1 had urticaria-like rashes,3 had dermatomyositis-like rashes,and 1 had prurigo pigmentosa-like rashes.Biopsies were carried out at the sites where transient rashes or persistent papules/plaques occurred.Histopathological findings showed necrotic keratinocytes in the upper prickle cell layer,and perivascular infiltration of neutrophils and lymphocytes in the upper dermis.Conclusion The skin lesions and histopathological changes of AOSD are characteristic,which can provide clues to the early diagnosis of AOSD.

Objective To analyze the clinical features and factors associated with knee osteonecrosis in patients with systemic lnpus erythematosus (SLE).Methods Medical charts of Peking Union Medical College Hospital from 2000 to 2013 were reviewed to identify patients who were diagnosed as SLE with kuee osteonecrosis.Controls were selected and matched to the cases by age and sex.The clinical and laboratory variables were compared between SLE patients who were with and without knee osteonecrosis.Numerical data and categorical data comparisons were analyzed using t-test,x2-test,respectively.Results Sixteen patients developed knee osteonecrosis that constituted 0.37％ of all the 4 301 hospitalized SLE patients during the same period.Fifteen patients were female,who developed knee osteonecrosis with an average age of 36 years (range:17-67 years).The mean duratiou of SLE before the diagnosis of knee osteonecrosis was 64±40 months (range:16-120 months).The interval from onset of symptoms to diagnosis was 0.5 to 24 months.The distal end of femur was involved in all 16 patients,while the proximal tibia necrosis also occurred in 8 patients.The detection rate of knee osteonecrosis by MRI was 100％,while it was only 19％ by plain radiography.The incidence of skin vasculitis,myositis,osteoporosis,hyperlipidemia,hypoalbuminemia and elevated serum immunoglobin G levels were significantly higher in SLE patients with knee osteonecrosis than in controls (P＜0.05).In addition,the use of anti-malarials was significantly lower in SLE patients with knee osteonecrosis than in controls (P＜0.05).Conclusion The knee osteonecrosis in SLE patients is affected by multifactors.The risk factors include skin vasculitis,myositis osteoporosis,hyperlipidemia,hypoalbuminemia and the elevated immunoglobin G level.The use of antimalarial drugs in SLE patients may protect against knee osteonecrosis development.Knee osteonecrosis coull be diagnosed early by MRI.

Objective To analyze the risk factors and clinical outcome of contrast induced nephropathy (CIN) in patients with coronary artery disease (CAD) after percutaneous coronary intervention (PCI) and discuss its prevention.Methods Fifty-four patients with CIN among 729 patients who received PCI were retrospectively studied and the related risk factors,cardiovascular events and preventive strategy were analyzed.Results CIN was strongly associated with pre-procedure chronic renal failure,diabetes mellitus and large-dose contrast.The incidence of cardiac mortality and major adverse cardiac events 1 year after PCI in CIN group was higher than that in group without CIN.Conclusion Chronic renal failure,diabetes mellitus and dosage of contrast agent were three independent risk factors of CIN.CIN could affect the patients' prognosis.A well overall perioperative management of CAD patients following PCI,especially hydration therapy,is the most important strategy for prevention of CIN .

Objective To investigate the relationship between the level of 8-iso-prostaglandin (8-iso-PG)F2αand the neural functional deficit in patients with acute cerebral infarction (ACI). Methods Sixty-seven ACI patients were se-lected in Neurological Department of Ganyu People’s Hospital. According to the age, these subjects were divided into two groups:the old group (≥60 years, n=37) and middle-young group (<60 years, n=30). Thirty healthy subjects were selected as controls (≥60 years). The age, gender and anamnesis were matched in two groups of elderly people. The ELISA was used to detect the plasma levels of 8-iso-PGF2αin two groups of patients. And NIHSS score was used to evaluate the severity of clinical neurological deficit. Results The plasma levels of 8-iso-PGF2α were significantly higher in old ACI group (506.38±138.63) ng/L than those of middle-young ACI group (420.18±132.72) ng/L and old control group (369.98±99.81) ng/L. There was no significant difference in plasma level of 8-iso-PGF2αbetween middle-young ACI group and old control group (F=9.272,P<0.05). The NIHSS score was significantly higher in old group (19.78±3.66) than that of middle-young group (17.73 ± 2.70, t=2.539,P<0.05). There was a positive correlation between plasma 8-iso-PGF2α level and NIHSS score in old group (r=0.504,P=0.001). Conclusion The oxidative stress plays an important role in the occurrence and de-velopment process of ACI in elderly patients. The earlier and reasonable antioxidant therapy plays a positive role to alleviate the clinical symptoms and promote the recovery of illness.

Objective To explore the effect of predepositautotransfusion in hematopoietic stem cell donors.Methods 127donors of hematopoietic stem cell wereretrospectively analyzed from 2013 to 2016,whenhematopoietic stem cells collection and autologous blood transfusion.Results Collection autologous blood 70 550 mL by 127 donors,and 2 cases had mild blood donation reaction,at a rate of 1.6％ (2/127).Collectionbone marrow stem hematopoietic stem cell 100 159 mL.The detection results were WBC (27.02±8.21) × 1010/L、Plt(213.19±62.42) × 109/L、RBC(4.31±0.44) × 1012/L、Hb(131.53 t14.53)g/L,from 117 donors beforespinal cord extraction.The detection results were WBC (35.8519.08)× 1010/L、Plt (188.34±50.24) × 109/L、RBC (4.02± 0.41) × 1012/L、Hb (122.60 ± 13.48) g/L,from 117 donorsafter autologous blood transfusion in one day.Compared with donors after autologous blood transfusion in one day,WBC was higher before spinal cord extraction(P＜0.05),Plt RBC and Hb was significantly decree (P＜0.05).Conclusion Preoperative autologous blood donation can save blood and prevent blood-borne infectious disease,which can used in Hematopoietic stem cell donor.