Objective To investigate postoperative infection rates of patients with different types of incisions and different risk indexes of operation in a tertiary hospital in Chengdu City through carrying out targeted monitoring of surgical incision,in order to determine important interventions for infection control.Methods The data about operating rooms in the hospital monitored in this study was collected via the Donghua software electronic information system,and the situation of postoperative infection of incision wounds was analysed as well.Results Among the 1 331 cases of patients,the total infection rate of postoperative incisions was 0.60％.The type Ⅰ,type Ⅱ and type Ⅲl/Ⅳ incision infection rate was 0.31％,0.69％ and 1.78％,respectively.The infection rate of patients whose surgical risk score was 0,1 point and ≥2 points was 0.13％,0.84％ and 1.74 ％,respectively.By Fisher exact test,there were statistically significant differences in postoperative infection rates among patients with different types of surgical incision and those with different surgical risk index scores(P＜0.05).Conclusion The postoperative infection rates of patients with type Ⅲ/Ⅳ incision and those with surgical risk index score≥2 points are the highest.It is necessary to emphasize and strengthen the intervention on controlling postoperative infections,effectively reduce the postoperative infection rate through active monitoring,in order to improve the quality of medical hospital treatment and ensure patients'safety.

Objective To evaluate the efficacy of embryo thymus transplantation in the treatment of lupus-like BXSB mice,study the pathogenesis of SLE in BXSB mice and the therapeutic effect of embryo thymus transplantation.Methods The embryonic thymus of CB57L mice was transplanted to50day-old male BXSB mice.Levels of proteinuria,ANA,blood urea nitrogen(BUN),blood creatinine and the deposits of immunoglobulin(Ig)in the glomerulus were detected regularly for5months,and the number of mice died of SLE was observed.Results The levels of proteinuria,ANA,BUN,blood creatinine of the5,6,7month old mice in embryo thymus transplantation group were lower than that of5month old mice in control group.The efficacy of treatment in embryo thymus transplantation group were similar to that of dexamethasone treatment group,and the SLE-caused death was reduced in these two groups.However,the embryo thymus transplantation seemed not to reduce the deposits of lg in glomerulus significantly,the deposits of lg in the glomerulus were similar in all three groups.Conclusions Embryo thymus transplantation could improve renal functions and reduce the titer of ANA.Its efficacy is similar to that of dexamethasone.Embryo thymus transplantation has a short term therapeutic effect in the treatment of lupus-like BXSB mice.The deficiency of thymus in the BXSB mice may play an important role in the pathogenesis of lupus-like mice.Embryo thymus transplantation may be a valuable approach to treat SLE.

Objective To explore the expression of tankyrase (TNKS) and its relationship with WNT/β-catenin signal?ing pathway in lung acinar adenocarcinoma. Methods Seventy-two samples of single subtype alveolar like lung adenocarci?noma (lung adenocarcinoma group) and 67 specimens of normal lung tissue adjacent to carcinoma (adjacent to carcinoma group) were collected. Immunohistochemical method was used to detect expressions of TNKS, beta-catenin (β-catenin) and c-myc protein. The correlation of each protein expression in lung adenocarcinoma tissues was analyzed. The differential ex?pression of TNKS was detected by Western blot assay in two groups. Results Tankyrase protein was mainly expressed in cy?toplasm. The expression ofβ-catenin protein was mainly in cytoplasm and nuclear of lung adenocarcinoma. The expression ofβ-catenin was mainly in cytoplasm, and a small amount was in nuclear of the adjacent group. The c-myc protein was ex?pressed mainly in the nucleus. The positive expression rates of TNKS,β-catenin and c-myc protein were significantly high?er in lung adenocarcinoma group than those of adjacent to carcinoma group (P<0.05). The expression ofβ-catenin in cyto?plasm and nucleus was positively correlated with the expression of TNKS and c-myc (P<0.05). Western blot analysis showed that the relative expression level of TNKS was significantly higher in lung adenocarcinoma group than that of adja?cent to carcinoma group (0.497 ± 0.021 vs. 0.237 ± 0.015, t=13.00, P<0.01). Conclusion Abnormally high expression of TNKS in lung adenocarcinoma may promote the occurrence of lung cancer by regulating the WNT signaling pathways. Inhib?iting TNKS expression may become a new target to treat lung adenocarcinoma.

OBJECTIVESProgrammed death-1 (PD-1) up-regulation impairs virus-specific CD8+ T-cell responses during chronic viral infection. Whether PD-1 expression influences the virus-specific CD8+ T cells in humans with acute viral infection remains largely undefined. This study aims to characterize the PD-1 expression during acute hepatitis B (AHB), and further addresses the association between the PD-1 dynamics and memory T-cell formation during acute HBV infection.

METHODSPeripheral HBV-specific CD8+ T cells from 11 HLA-A2-positive AHB patients were longitudinally quantitatively analyzed, and PD-1, memory markers CCR7, CD45RA and CD127 and activation marker CD38 on HBV-specific CD8+ T cells were measured using flow cytometric assay. Serum ALT, HBsAg, HBsAb and HBV-DNA levels were evaluated for each subject.

RESULTSAll 11 AHB patients examined had multiple pentamer-positive CD8+ T-cell responses in their early phase of HBV infection. Specifically, their PD-1 on pentamer-positive CD8+ T-cells was significantly up-regulated at the onset of their disease. Following their disease resolution, the dynamic decrease in PD-1 expression was found to correlate with the phenotypic development of memory CD8+ T cells, indicated by the increases in CCR7, CD45RA and CD127 and decrease in CD38.

CONCLUSIONPD-1-mediated negative signaling may be closely associated with memory T-cell formation during acute self-limited hepatitis B.

Acute Disease ; Adult ; Antigens, CD ; metabolism ; Apoptosis Regulatory Proteins ; metabolism ; Female ; Hepatitis B ; immunology ; metabolism ; Humans ; Immunologic Memory ; Male ; Middle Aged ; Programmed Cell Death 1 Receptor ; T-Lymphocytes, Cytotoxic ; immunology ; metabolism ; Young Adult

OBJECTIVETo examine the expression of Osterix (Osx) mRNA and protein after application of mechanical force on human periodontal ligament cells (HPDLCs), and to investigate the role of Osx in orthodontic alveolar bone remodeling.

METHODSHPDLCs were isolated and cultured in vitro with explant method. Approximately 2.5 x 10(5) cells were seeded onto six-well cell culture plates and then were exposed to centrifugal force for 1, 2, 4, 6, 8 or 12 h at 631 r x min(-1). The expression of Osx mRNA and protein was measured by reverse transcription-polymerase chain raction (RT-PCR) and Western blot respectively. Immunofluorescence assay was used to detect the expression and subcellular At the initial time point, Osx mRNA had a weak exlocalization of Osx protein by green fluorescence.

RESULTSpression and protein was not detected. Under the mechanical stimulation, both mRNA and protein levels of Osx were upregulated in a time-dependent manner. Furthermore, Osx protein was translocated gradually from the cytosol into the cell nuclei.

CONCLUSIONThe expression and activation of Osx were enhanced by mechanical stress in HPDLCs, which indicates that Osx may play an important role in HPDLCs osteogenic differentiation and periodontal tissue remodeling induced by mechanical stress.

Bone Remodeling ; Cell Culture Techniques ; Cell Differentiation ; Cell Line ; Humans ; Osteogenesis ; Periodontal Ligament ; RNA, Messenger ; Stress, Mechanical

OBJECTIVETo investigate the expression of ghrelin and its receptor, growth hormone secretagogue receptor (GHS-R), in the hypothalamus and gastrointestinal tract in rats with chronic renal failure (CRF) and explore their relationship with the disorder of gastrointestinal tract motility.

METHODSSD rats were randomly divided into sham-operated group (n=8) and CRF group (n=16), and in the latter group, the rats were subjected to 5/6 nephrectomy to induce CRF. Real-time PCR and immunohistochemical staining were used to detect the distribution of mRNA and protein of ghrelin and GHS-R in the gastric fundus, duodenum, and hypothalamus.

RESULTSThe rats in the CRF group showed a significantly higher expression of ghrelin mRNA and protein in the gastric fundus but a lower expression in the hypothalamus than those in the sham-operated group (P<0.01), but the expression in the duodenum was similar between the two groups (P>0.05). The expression of GHS-R mRNA and protein in the gastric fundus was significantly higher in the CRF group than in the sham-operated group (P<0.01), while in the hypothalamus and duodenum, the expression was significantly lower in the CRF group (P<0.01).

CONCLUSIONThe different distribution patterns of ghrelin and GHS-R in the tissues may be an important pathological basis of gastrointestinal motility disorder in CRF.

Animals ; Gastrointestinal Tract ; metabolism ; Ghrelin ; genetics ; metabolism ; Hypothalamus ; metabolism ; Kidney Failure, Chronic ; metabolism ; Male ; RNA, Messenger ; genetics ; metabolism ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Receptors, Ghrelin ; genetics ; metabolism

Objective To learn the epidemic characteristics of metabolic syndrome (MS)among rural adults in Changshan County and to provide evidence for developing control and prevention measures.Methods A total of 1 21 2 residents aged over 1 8 years old in four towns were selected by multi -stage stratified cluster random sampling method and an investigation including questionnaire,physical examination and blood biochemical examination was conducted.Results The crude prevalence rate of MS among rural adults in Changshan County was 30.61 % and the standardized prevalence rate was 26.32%.The MS prevalence rate was significantly higher in females than in males (P <0.01 ).The major risk factors for MS were female gender (OR =1 .98),elderly age (OR =1 .02)and family history of hypertension (OR =1 .60). Conclusion The prevalence rate of MS is considerably high in rural areas of Changshan County and effective measures should be taken at early stage.

OBJECTIVETo investigate the value of 18F-FDG PET-CT in detecting metastatic lymph node and radiation planning for patient with non-small-cell lung cancer (NSCLC).

METHODSThe data of 58 NSCLC patients were retrospectively analyzed. Preoperatively, Both 18F-FDG PET-CT and CT scan were performed for all patients. Blinded interpretation of these images was then carried out. The gross tumor volume defined by radiation oncologist based on imaging results of either PET-CT or CT alone was compared with pathological results eventually.

RESULTSThe sensitivity, specificity, accuracy, positive predictive value and negative predictive value of detecting metastatic lymph node in the mediastinum was 56.0%, 54.2%, 54.8%, 38.9%, 70.3% by CT alone, and 88.0%, 85.4%, 86.3%, 75.9%, 93.2% by PET-CT, respectively. There was a statisticalty significant difference between two methods(P < 0.05). However, statisticalty no significant difference in detecting hilar lymph nodes was observed. The radiation target volume defined by CT was identical to that by PET-CT in 31(53.5%) cases, but changed in the other 27 (46.5%) cases when 18F-FDG PET data was added. The accuracy was 75.9% in detecting metastatic lymph nodes in the radiation target volume defined by PET-CT, while it was much lower by CT alone (48.3%) with a statistically significant difference between two methods (P < 0.05).

CONCLUSION18F-FDG PET-CT is more accurate than CT alone in assessment of mediastinal lymph nodes for NSCLC patients. It may be recommended as a method in defining the radiation target volume.

Adult ; Aged ; Aged, 80 and over ; Carcinoma, Non-Small-Cell Lung ; diagnostic imaging ; pathology ; radiotherapy ; Female ; Fluorodeoxyglucose F18 ; Humans ; Lung Neoplasms ; diagnostic imaging ; pathology ; radiotherapy ; Lymph Nodes ; diagnostic imaging ; pathology ; radiation effects ; Lymphatic Metastasis ; Male ; Mediastinum ; diagnostic imaging ; radiation effects ; Middle Aged ; Positron-Emission Tomography ; methods ; Radiopharmaceuticals ; Retrospective Studies ; Tomography, X-Ray Computed

As a therapeutic agent in thrombosis the fibrinolytic enzymes are of interest and the search for a new enzyme continues. A novel fibrinolytic enzyme was produced from Rhizopus chinensis 120, which was screened from the starter for brewing rice wine in the South of China, by solid fermentation, and purified through ammonium sulfate precipitation, hydrophobic interaction, ionic exchange and gel filtration chromatographies. The purified enzyme hydrolyzed fibrin, it cleaved the alpha-, beta- and gamma-chains of fibrinogen simultaneously, and it also activated plasminogen to plasmin. The enzyme hydrolyzed N-Succinyl-Ala-Ala- Pro-Phe-pNA, and Km was 0.23 mmol/L and Kcat 16.36 s(-1). The optimal temperature of the enzyme for hydrolying fibrin was 45 degrees C, and the optimal pH range of 6.8 - 8.8. The isoelectric point of the enzyme estimated by isoelectric focusing electrophoresis was 8.5 +/- 0.1. The enzyme was a glycoprotein. EDTA, PCMB, PMSF inhibited the activety of the enzyme, and SBTI, Lys, TPCK, Aprotinine had none obvious inhibition, which suggested that the activity centre of the enzyme had hydrosulfuryl, metal and serine. The first 12 amino acids of the N-termimal sequence of the enzyme were NH2-Ser-Val-Ser-Glu-Ile-Gln-Leu-Met-His-Asn-Leu-Gly, and had none homology with that of other fibrinolytic enzyme from other microbes. The novel fibrinolytic enzyme from Rhizopus chinensis 12# has potential to become a therapeutic agent in thrombosis.
Enzyme Stability ; Fermentation ; Fibrinolysin ; metabolism ; Fibrinolysis ; Fibrinolytic Agents ; chemistry ; Humans ; Plasminogen ; metabolism ; Rhizopus ; enzymology

The aim of this study was to establish the culture system of isolation and cultivation of the neural stem cells (NSCs) from the embryonic rat brain and spinal cord. The methods of microscopic dissection, cell culture and immunofluorescence cytochemistry were used. The results are as follows. (1) In the presence of fibroblast growth factor-2 (FGF-2) and epidermal growth factor (EGF), both brain- and spinal cord-derived stem cells proliferated and expanded in vitro for 8 - 10 passages (over 60 d). The period of expansion resulted in a 10(6)-fold increase in brain-derived NSCs and 10(5)-fold increase in spinal cord-derived NSCs. These proliferating cells expressed nestin. (2) In the medium containing 1% FBS, the two NSCs populations could be induced to differentiate into neurons, astrocytes and oligodentrocytes. The percentage of neurons (beta-tubulin III-ir) differentiated from brain-derived NSCs decreased rapidly from 11.95+/-2.5% at passage 2 (P(2)) to 1.97+/-1.16% at passage 5 (P5). Significant difference was shown between P(2) and P(5) (P<0.01). The percentage of oligodentrocytes (Rip-ir) differentiated from brain-derived NSCs remained mostly unchanged from 8.66+/-2.93% at P(2) to 9.12+/-1.13% at P(5). The same differentiation patterns were found in spinal cord-derived NSCs. All these results indicate that both embryonic rat brain- and spinal cord-derived NSCs can expand and proliferate in vitro through multiple passages, and retain the capacity to differentiate into all three major types of cells in the central nervous system.
Animals ; Brain ; cytology ; Cell Culture Techniques ; methods ; Cell Separation ; Cells, Cultured ; Embryo, Mammalian ; Embryonic Stem Cells ; cytology ; Female ; Neural Stem Cells ; cytology ; Pregnancy ; Rats ; Rats, Wistar ; Spinal Cord ; cytology