Objecttve To investigate the effects of snfentanil and ropivacaine used in labor combined spinalepidural analgesia(CSEA)and its influence on labor course and infants and its side effects.Methods 160 primiparas who chose deliver analgesia were randomly divided into two groups:group A intrathecal 5μg sufentanil plus saline up to 2.5ml,extradural 0.2μg/ml sufentanll plus 0.1% ropivacaine and saline up to 100 ml;group B extradural ropivacaine 1.5mg plus glucose 100g/L up to 2ml;group C have no analgesia.Then to investigate the effects of analgesia VAS scores,labor course duration,caesarean section rate,flooding volume,neonatal Apgar scores and its side effects.Results Compared with group C,the analgesia effects of group A and B were definitely(P＜0.05,P＜0.01);visual analogue score(VAS)at 5min,10min and 30min in group A were(3.4±1.2),(1.6 ±0.7)and(0.7 ±0.5)respectively,while in group B were(5.3±1.5),(4.3±1.8)and(3.1±1.7),which had significant difference in two groups(all P＜0.01);they all could reduce the first labor course duration,caesarean section rate,and there were no difference with group C(P＞0.05);and the flooding volume and neonatal Apgar scores had no differences(P＞0.05).Conclusion Sufentanil and ropivacaine combined used in CSEA has stronger effect and longer duration without prolonged labor coulee and poor side effects,which would be a more perfect analgesia worth clinical application in obstetrics.

Objective To analyze the data collected from surveillance sites in Shanxi province in epidemiological investigations during 2004 - 2008 and understand the influence of such factors as rat density and vectorial quantities on plague epidemic intensity, thus provide some scientific basis for plague control and prevention. Methods To collect the data of surveillance during 2004 - 2008 in the 12 counties of plague surveillance by retrospective method. Sort and analyze the collected data according to the requirement of operational scheme for plague surveillance-calculate the rat density of spermophilus dauricus, meriones meridianus and the small rodents, and the flea index and the flea infection ratio of body flea, burrow track, cave. The results of serological tests were analyzed at the same time. Results As showed in the results, the mean density of spermophilus dauricus was 1.12 merely in each hectare, and that of meriones meridianus and small rodents were 7.42%(2130/28 700) and 8.03%(3886/48 400), respectively. The flea infection rate of spermophilus dauricus and meriones meridianas were 25.78% ( 1933/7497 ) and 44.19% (2028/4598), respectively;the flea index of spermophilus dauricus and meriones meridianus were 0.59 and 1.84, respectively. The number of nests excavated with flea for spermophilus dauricus was 70, and the numbers of nests and flea were 362 and 333, respectively. The flea infection rate was 19.34 % (70/362), and the flea index was 0.92. The number of nests excavated with flea for meriones meridianus was 34, and the numbers of nests and flea were 177 and 124, respectively. The flea infection rate was 19.21%(34/177), and the flea index was 0.70. The number of caves explored for spermophilus dauricus was 7171,and the number of burrows with flea was 62, so the flea infection rate was 0.86%(62/7171 ), and the flea index was 0.01. Conclusions With a relatively high density and large quantity and considering the epidemic features of animal plague in periphery provinces in recent years, plague may still happen and spread in Shanxi province diespite the negative results of all those serological tests, by means of importing from other provinces.

Objective To study the clinical efficacy of recombinant human epidermal growth factor (rhEGF) in the treatment of sacrococcygeal decubitus ulcers grade Ⅲ with direct polymerizing suture after appling rhEGF to reinforce wound bed preparation (WBP). Methods From January 2007 to October 2009, 60 patients with sacrococcygeal decubitus ulcers Grade Ⅲ, were divided into control group and treatment group. The ulcer size was 3 cm × 4 cm to 10 cm × 12 cm and all ulcers were infected for 15-70 d. Treatment group received traditional dressing change and appling rhEGF to reinforce wound bed preparation. Control group received traditional dressing change only. The operative technique that we used in two groups was direct polymerizing suture. Cure rate of stage Ⅰ and complication morbidities were analyzed. Results Cure rate of stage Ⅰ was 87% in treatment group and 70% in control group. Complication morbidities were 13% in treatment group and 30% in control group. The difference between these two groups was statistically significant (P ＜ 0.05). Conclusion Appling rhEGF to reinforce wound bed preparation before operation could make subsequent treatment more effective and improve the cure ratio of operation with decreasing complications and morbidities. And more, dissecting under fascia possesses the advantages of easiness to perform and rich blood supply.The method of appling rhEGF with direct polymerizing suture is a simple, high efficient approach for the first repairment of sacrococcygeal decubitus ulcers grade Ⅲ, especially desirable for the elderly.

0.05). Conclusion Oral administration of prednisone can prevent modrate and severe OHSS and may have no effect on pregnant rate of IVF.

Objective To explore the feasibility of extending liver blood perfusion cessation by ultrasound combining microbubbles.Methods The livers of 9 healthy rabbits were treated with a pulsed therapeutic ultrasound device,in presence of microbubbles.For quantification of liver perfusion,contrastenhanced ultrasonography was performed on 6 rabbits before treatment and at different time points of 0 min,30 min,60 min and 48 hours after treatment.Pathological examination of the treated livers was performed immediately after treatment on the other 3 rabbits.Results The liver blood perfusion almost vanished immediately after treatment,remained at a low perfusion level from 30 to 60 min,and completely recovered 48 hours later.The peak intensity dropped from ( - 51.88 ± 4.26)dB to ( - 62.53 ± 4.83)dB after treatment and rose up to ( - 52.00 ± 4.60) dB 48 hours later.The peak intensities before treatment and 48 hours after treatment were significantly higher than those of 0 min,30 min and 60 min time points after treatment( P ＜0.05).Pathological examination showed significant swelling of hepatocytes and hemorrhage around portal veins.Conclusions Microbubbles enhanced ultrasound can induce liver blood perfusion cessation for up to 1 hours.The mechanism could be swelling of hepatocytes and hemorrhage of portal track.

AlM:To evaluate the efficacy of combined pranoprofen eye drops and artificial tears on the treatment of mild to moderate dry eye syndrome after trabbeculectomy.METHODS:This prospective case control study included 63 cases (63 eyes) of patients with mild to moderate dry eye syndrome after trabbeculectomy in our hospital from November 2013 to June 2013. All subjects were randomly divided into two groups. Observation group was treated with combined pranoprofen eye drops and artificial tears and control group received simple artificial tears marking the eyes at 1, 2, 4wk. The patient's symptoms, signs, BUT, S▏t, and FL were observed before treatment and 1, 2, 4wk after treatment.RESULTS: After 2wk, the symptoms of observation group were improved, there was statistically significant difference (P<0. 05). FL difference of each group was statistically significant ( P<0. 05 ); After 4wk, symptoms and signs were improved. There was statistically significant difference ( P < 0. 05 ). The BUT of the observation group and corneal FL scores of two groups showed significant differences (P<0. 05). CONCLUSlON: Artificial tears joint pranoprofen eye drops has good curative effect in the treatment of mild to moderate dry eye syndrome after trabbeculectomy.

Objective To study the protective effect and mechanism of Inonotus obliquus polysaccharide on liver injury induced by isoniazid( INH) combined with rifampicin( RFP) in mice. Methods Forty-eight mice were randomly divided into six groups ( control, model, huganpian, Inonotus obliquus polysaccharide at low, middle, and high dose ) . The mice were administered orally with isoniazid and rifampicin simultaneously except the control. After 2 h,the control and model groups were administered with normal saline,and others were treated with huganpian and Inonotus obliquus polysaccharide,respectively,once a day for 2 weeks. The liver index and serum levels of ALT and AST,malondialdehyde(MDA),superoxide dismutase(SOD) and glutathione peroxidase( GPx) were measured. Pathology examination of liver tissue was performed. Results The activities of ALT and AST, liver index, content of MDA in liver homogenate of mice treated with Inonotus obliquus polysaccharide and huganpian decreased obviously, while the liver SOD activity increased. Histopathological exzamination showed that Inonotus obliquus polysaccharide alleviated the degeneration and necrosis of hepatic cells. Conclusion Inonotus obliquus polysaccharide shows protective effects on mice with hepatic toxic injury induced by isoniazid and rifampicin,which maybe due to its antioxidation effect.

OBJECTIVETo explore the effect of internal fixation with screw through femoral epiphyseal plate on growth in- hibition via an experimental study.

METHODSForty New Zealand rabbits were randomly divided into 4 groups and 10 rabbits in each group. Epiphyseal plate was injured by penetrating of screws, and the size of damage area was controlled by changing the number of threads. Group A: blank group; group B: injury area accounted for 4% of the epiphyseal plate; group C: injury area accounted for 6%; group D: injury area accounted for 8%. The internal fixation was removed after 2 weeks, and the results were observed with X-ray film for 4 groups to judge the complications such as early closure of epiphyseal.

RESULTSIn each group, there were no statistical differences in the length of the femoral neck, the diameter of femoral neck, the diameter of the femoral head, and the epiphyseal plate closure time. The growth speed of the length and diameter of the femoral neck, as well as the diameter of femoral head, were quicker on the early phase, and the speed was slowest when the epiphyseal plate was being closed.

CONCLUSIONThe injury area of epiphyseal plate under 8% is safe for its growth. Because no evidences demonstrate the growth inhibition of epiphyseal plate, the screws can be used for rabbit epiphyseal plates.

Animals ; Bone Screws ; Female ; Femur Head ; growth & development ; surgery ; Fracture Fixation, Internal ; methods ; Growth Plate ; growth & development ; Magnetic Resonance Imaging ; Male ; Rabbits ; Salter-Harris Fractures

Objective To investigate the correlation of cyclophilin A (CyPA) level in patients with essential hyperten?sion(EH)in Xinjiang Xibo and Mongolia populations and to investigate its clinical significance. Methods EH patients (n=76) and healthy control subjects (n=62) were selected from Xinjiang Xibo populations. On the other hand, EH patients (n=66)and healthy control subjects (n=57) were selected from Xinjiang Mongolia populations. Serum cyclophilin A was measured using enzyme-linked immunosorbent assay(ELISA). Parameters such as age, body mass index(BMI), waist, waist hip ratio, blood pressure were measured in each group. The correlation of cyclophilin A with each parameter was analyzed. Results The levels of serum cyclophilin A in EH patients in Xinjiang Xibo and Xinjiang Mongolia populations were significantly high?er than those in their corresponding healthy control groups(P<0.05). Serum cyclophilin A in patients with essential hyperten?sion(EH)in Xinjiang Mongolia population was signifantly higher than that in EH patients from Xinjiang Xibo population (P<0.05). There was a positive correlation between serum cyclophilin A level and occurance of EH in both Xinjiang Xibo and Xinjiang Mongolia populations. Conclusion The serum cyclophilin A is significant higher in EH patients in both Xinji?ang Xibo and Xinjiang Mongolia populations so it is positively correlated with occurance of EH (P<0.05). Cyclophilin A lev?el is a risk factor of essential hypertension in both Xinjiang Xibo and Xinjiang Mongolia populations.

Animals ; China ; epidemiology ; Epidemiologic Studies ; Molecular Epidemiology ; Rickettsia ; genetics ; isolation & purification ; Rickettsia Infections ; epidemiology ; transmission ; Ticks ; microbiology