Objective To observe the effect and safety of modified Yishen Zhuyang Huoxue Huazhuo Formula for polycystic ovarian syndrome (PCOS) in the TCM pattern of deficiency of spleen and kidney with phlegm-dampness. Methods Totally 123 cases were recruited into this study and randomized to two groups by central random approach, 64 cases in the observation group and 59 cases in the control group. Patients in the observation group were given the modified Yishen Zhuyang Huoxue Huazhuo Formula, while those in the control group were given compound cyproterone acetate tablet, for two treatment courses, three months for one course. The changes of ovarian volume and the number of follicles before and after treatment were observed. Adverse reactions were also evaluated. Results Bilateral ovarian volumes in both groups decreased after treatment (P<0.05, P<0.01), while the control group showed a significant better improvement (P<0.05). The number of follicles in the two groups decreased, but no significant difference were observed between the two groups (P>0.05). The rate of adverse reaction in the observation group was lower than the control group. Conclusion Modified Yishen Zhuyang Huoxue Huazhuo Formula is effective for PCOS patient by reducing the ovarian volume with a low incidence of side effect.

BACKGROUND:Numerous studies have demonstrated that mild hypothermia has a better protective effect on neurons after cerebral infarction.
OBJECTIVE:To investigate theeffect of mild hypothermia on nerve regeneration microenvironment of infarcted area in rat models of cerebral infarction and analyze its possible effects on neural functional recovery after cerebral infarction.
METHODS:Twenty out of 65 adult femaleSprague-Dawleyrats were randomly selected as the sham group. The remaining 45 rats were subjected to carotid artery ligation to establish rat models of cerebral infarction. Five rats were rejected because of modeling failureor death, the remaining 40 rats were randomly and evenly divided into cerebralinfarction and mild hypothermia groups. The head temperature of rats in the cerebral infarction group was downregulated to (37±1)℃ using a semiconductor refrigeration instrument. The rats were transferred to the room with the temperature of 25℃ after the operation. Brain hypothermia was also induced in rats from the mild hypothermia group. At 13.0-14.0 minutes after establishing rat models of cerebral ischemia, the head on the side of cerebral ischemia was tightly connected with the probe of the semiconductor refrigeration instrument. The refrigerator temperature was adjusted to 6-8℃, so as to make the temperature of brain tissue on the lesion side at 32.0-33.0℃ for 4 hours.
RESULTS AND CONCLUSION:Compared with the cerebral infarction group, the BBB scores of rats inthe mild hypothermia group were distinctly increased, and the volume of infarcted area decreased. At 1 day after modeling, the expression level of growth associated protein 43 mRNA in brain tissue of rats in the mild hypothermia group was close to that in the cerebral infarction group. At 2 weeks after modeling, the expression level of growthassociated protein 43 mRNAin brain tissue of rats in the mild hypothermia group was significantly increased compared with that in the cerebral infarction group. These results suggest that mild hypothermia therapy can protect nerve cels against injury caused by cerebral infarction and promote the recovery of neurological function. Its underlying mechanism may be related to the up-regulation of the expression of growth associated protein 43 in ischemic penumbra .

Objective To establish a method for determination of trace phenol in waste water. Methods It is reported that trace amounts of phenol can inhibit the decoloring reaction of methyl red by potassium bromate in dilute sulfuric acid medium. Results The linear range of this method was 0-17 ?g/25 ml for phenol with detection limit of 6.53?10-9 g/ml, RSD was 0.96%-1.70%, recovery rate was 97.8%-102.0%. Conclusion The proposed method is simple, sensitive and can be applied to determine trace phenol in waste water with satisfactory results.

Bursa of Fabricius is a very important organ for B-eell development. We sot bursa extracts from 2-3 months old Peking Duck. A partially purified PFC-stimulating active factor has been seperated from the extracts by twice molecular sieve chromatography, Sephadex G-15 and Moselect G-10. The M. W. of the active factor is below 750 dal. The results of HPLC analysis of the factor show that its polarity is relatively strong, and it has more positive charges.The works of purifying and looking for a new assay are still going on.

Bursa of Fabricius is the central organ of B-cell development in birds. There are some active factors in Bursa that are involved in the maturation of B lymphocytes. Bursa extracts from 2-3 month-old peking Duck, with a M. W. of 10,000 dal, can obviously increase the number of plaque-forming cells (PFCs) in normal or cyclophosphamidetreated mouse spleen. Cyclophosphamide (CP) is a kind of B-cell inhibitor. The extracts can also increase the life expectation of mouse treated with deathal dose of CP. These results show that Bursa extracts contain a factor (or some factors) which can promote the development or function of B cells. The factor is named as Bursatin. Because the PFCs-stimulating activity of the factor is susceptible to high temperature and pronase E, Bursatin is infered to be a peptide. The effect of Bursa extracts on elevating the cAMP level of mouse spleen cells suggests that Bursatin may function via cAMP. Besides, it is interesting that we have found that the extracts can also increase the rumber of E-rosett forming cells in pig thymocytes. The result shows that there are several active factors or certain factor poesses different activities in Bursa extracts.

OBJECTIVE: To compare the in vitro dissolution of carvedilol tablets from four different pharmaceutical factories for references of clinical application of carvedilol tablets. METHODS: The dissolution test was carried out with basket-rotating method. The content of carvedilol was determined by UV spectrophotometry. The accumulative dissolution percentage was calculated. The Weibull’s equation was used to fit the dissolution parameters including T50,Td,T80,and m. Then an ANOVA was performed on these parameters. RESULTS: The dissolution of carvedilol from four manufactories conformed to the requirements of Chinese Pharmacopoeia(2005 edition). But significant differences were found among carvedilol preparations from different factories in dissolution parameters including m,T50,Td and T80(P

Objective To observe the clinical effect of point-to-point acupuncture with electric stimulation of direct current induction for facial nerve paralysis. Methods A total of 65 patients with facial nerve paralysis were randomly divided into the observation group (n=33) and the control group (n=32). The control group received routine treatment, and observation group was received Dicang (ST4)-to-Jiache (ST6) and Yangbai (GB14)-to-Taiyang (EX-HN5) by acupuncture with electric stimulation of direct current induction combined with conventional western medicine. The treatment of both groups last 3 weeks. The House-Brackmann Scale and the BR indexe at baseline and after 3 weeks treatment were observed. Results After treatment, The clinical effect of observation group was 66.7%, which was significantly higher than control group 43.8% (U=2.175, P=0.013). After treatment, House-Brackmann facial nerve scale (Ⅰ level=15 cases, Ⅱ level=4 cases, Ⅲ level=3 cases) in the observation group was significantly better than that in the control group (Ⅰ level=5 cases, Ⅱ level=7 cases, Ⅲ level=2 cases; U=2.069, P=0.018). After treatment, R1 latency (12.28 ± 2.55 ms vs. 13.84 ± 3.07 ms, t=2.227) and R2 latency (32.28 ± 7.08 ms vs. 35.95 ± 5.11 ms, t=2.396) in the observation group were significantly shorter than those in the control group (P=0.030, P=0.020). Conclusions The point-to-point acupuncture with electric stimulation of direct current induction could reduce latency of R1 and R2 for facial nerve paralysis, and improve the clinical effect.

An improved method for the purification of 1,25-(OH)2D3-26,23-lactone was established by using one step of highperformance liquid chromatography (HPLC). The analytic column was Shim-pack NH2 (straight-phase type). The mobile phase was Methanol: Dichloromethane: Hexane = 4:26:70 and the flow rate was 2ml/min. An ultraviolet detector with 254nm was used. The retention time of 1,25-(OH)2D3, l,25-(OH)2D3-26,23-lactone and 1, 24, 25-(OH)3D3 werel2.10, 17.06 and 22,00 minutes respectively. This method can separate 1,25-(OH)2D3, l,25-(OH)2D3-26,23-lactone and 1,24,25-(OH)3D3 only by one step of HPLC and it is better than the old one which need two steps of HPLC for the separation of these substances.

Objective To evaluate the technology and application of dual-source CT urography. Methods 56 cases with clinical symptoms and dual-source CT urography were analyzed retrospectively through conventional plain scan, arterial phase scan, venous phase scan, which gained data were reconstructed 3D images of MPR, MIP, CPR and VR, and the im- ages were analyzed. Results The 3D images of kidneys, ureters and bladder were reconstructed successfully, which showed clearly the detail of the entire urinary tract and the local fine structure, morphology, taking shape and the relationship with the surrounding tissue through rotating and zooming. Conclusion Through the dual-source CT to scan the entire urinary tract is suitable for a variety of data acquisition of three-dimensional reconstruction, which can greatly enhance the focal positive rate and diagnosis rate.

Measurements were made through underwater weighing on the body compo-sition of 40 ordinary male students (Group 1) and another 50 majoring inphysical education (Group 2). Two simple equations were worked out forestimating the percentage of fat with figures of their skinfolds and circum-ferences. The average percentage of fat and lean body weight were found to be11.76% and 54.3 kg respectively for Group 1, and 9.83% and 59.7 kg forGroup 2. With F standing for the percentage of fat, t for triceps skinfold and s forsuprailiac skinfold, the equation derived from Group 1 was F=2.44523 +0.99009t + 0.46426s, and that derived from Group 2 was F=1.16283t+0.54526s--0.21702 In underwater weighing, the mean percentage of fat was 9.70 ? 2.19 with anormal amount of inhaled air and 9.24 ? 2.46 with a maximal amount. Theinsignificant difference indicates that the normal inhalation method is wor-kable and more acceptable to the subjects.