Objective Comparing the variability of four genes of HCMV in the population and the therapy process of single patient.Methods Using PCR to amplify the four genes and restriction enzyme to validate four fragments, These four genes are UL55,UL57,UL86 and UL122 whose coding products are glycoprotein B,single chain binding protein,main capsid protein and IE pp86 separately. Then we use AD169 to optimize the conditions of PCR and restriction enzyme. then we use these conditions to detect 200 blood specimens. Results The PCR positive rate of UL55 is 12.5% and restriction positive rate is 10.0%, which has marked difference with other 3 gene segments(P

Objective To evaluate the preoperative diagnostic procedures and treatment choice of primary tumor of the duodenum (PTD).Method The clinical data of 52 cases with PTD in the last 10 years was analyzed retrospectively.Results The correct diagnostic rate of auxiliary examination was:duodenal endoscopy of 90.5%,air barium double radiography of 80%.Six primary benign tumors of duodenum (PBTD)were resected completely with a 5 year's survival of 100%.Among the 46 cases of primary malignant tumors of duodenum (PMTD),28 cases underwent pancreatoduodenectomy,4 cases received segmental duodenectomy,the remaining 13 cases in which the tumors were unresectable were treated bypass.The resection rate was 69.6% and the 5 years' survival rate was 32.6%.Conclusions Duodenal endoscopy and air barium double radiography are mainstays for the diagnosis of PTD.Segmental duodenectomy and simple tumor resection are curable for PBTD; while for PMTD,the therapy of choice should be pancreatoduodenectomy.

Chronic Periodontitis ; diagnosis ; diagnostic imaging ; therapy ; Dental Scaling ; Follow-Up Studies ; Furcation Defects ; diagnosis ; diagnostic imaging ; therapy ; Gingival Hyperplasia ; diagnosis ; diagnostic imaging ; therapy ; Humans ; Male ; Middle Aged ; Periodontal Index ; Radiography, Panoramic ; Root Canal Therapy ; Tooth Loss ; diagnosis ; diagnostic imaging ; therapy ; Tooth, Artificial

The etiology of rotated maxillary central incisor is unclear, but it has a clear familial tendency. Except for the environmental factors, gene expression directly influences craniofacial growth and the types of malocclusion deformity. In this article, a case of 180° rotated left maxillary central incisor of a male with family history of the deformity was reported. Relevant literature was also reviewed.
Humans ; Incisor ; Male ; Malocclusion ; diagnosis ; Maxilla

Acupuncture Therapy ; methods ; standards ; Art ; China ; Humans

OBJECTIVE:To explore the clinical efficacy and safety of Yunkang granule combined with dydrogesterone in the treatment of early threatened abortion. METHODS:128 patients with early threatened abortion were randomly divided into single drug group and combined medication group,64 cases in each group. Single drug group received Dydrogesterone tablet with first dose of 40 mg,then 10 mg,q 12 h until vaginal bleeding;combined medication group additionally received 8 g Yunkang granule, tid,until symptoms disappeared. Human chorionic gonadotropin (HCG),clinical symptoms remission time and disappearance time,pregnancy/childbirth complications,pregnancy outcome and neonatal adverse outcome,and the incidence of adverse reac-tions in 2 groups were compared. RESULTS:After treatment,HCG in 2 groups significantly increased,and combined medication group was higher than single drug group,the differences were statistically significant(P<0.05);clinical symptoms remission time and disappearance time in combined medication group were shorter than single drug group,the difference was statistically signifi-cant (P<0.05). There were no significant differences in incidence of pregnancy/childbirth complications,pregnancy outcome and neonatal adverse outcomes in 2 groups (P>0.05). The incidence of adverse reactions in combined medication group was 10.9%, which was significantly lower than single drug group (40.6%),the difference was statistically significant (P<0.05). CONCLU-SIONS:Both dydrogesterone alone and Yunkang granule combined with dydrogesterone show good efficacy in the treatment of ear-ly threatened abortion,but the remission time and disappearance time of clinical symptoms and adverse reactions in combined medi-cation group are less than that in single drug group.

Objective To explore the differentially expressed genes of gastric cancer,matched normal gastric tissue and premalignant lesions. Methods The differentially expressed cDNA bands were isolated and identified by fluorescent differential display and then reamplified by PCR.After being cloned,all cDNA fragments were sequenced. Through BLAST,the results were compared with GenBank database for homology analysis. The expression of the cDNA fragment in different tissues was confirmed by Northern blot. SMART-RACE(rapid amplication of cDNA ends) was used to amplify the full length cDNA sequence. Bioinformatics analysis was performed to analysis its function. Results A differentially expressed cDNA fragment expressed lower in gastric cancers and premalignant lesions,no homology was found in GenBank. The novel cDNA fragment was given an accession number of EST(CD454989)in GenBank. A full length cDNA sequence of 1362 bp was acquired,who coded 267 amino acids,and was homologous with BAA91562.1,whose physiology function was unknown. Conclusion A differentially expressed gene was found and it may be involved in process of the gastric cancer.

Facing the lack of the health service management professionals'vocational adaptability in medical college,we should improve their ability of professional practice,communication skills,professional knowledge and awareness of social responsibility in order to achieve the training objectives.

Femurs of 206 Chinese adults (122 males and 84 females) were used for the present study. Their lengths, and torsional and inclinational angles were measured after Martin's method. The results are as follows:1. The Lengths of FemurThe average length of the femur in males is 43.68?2.02 cm. (43.77?2.02 cm. on the left side, and 43.61?2.04 cm. on the right), while that in females is 40.96?2.84 cm. (41.05?2.84 cm. on the left side and 40.93?2.84 cm. on the right). No significant difference has been found between the femoral lengths of both sides; however, there is a significant difference between both sexes.2. The inclinational angles of femoral neckTheir average value in the male is 128.46??6.58?(128.87??6.76?on the left side, and 127.46??6.60? on the right), but that in the female is 130.52??6.44?(130.88??6.10? on the left side, and 130.17??6.80? on the right). No significant difference exists between both sides; however, there is a significant difference between both sexes.3. The Torsional Angles of FemurTheir average value in the male is 11.95??8.89? (14.51??8.08? on the left side, and 9.20??7.75? on the right), whereas that in the female is 12.80??9.07? (15.95??9.63? on the left side, and 9.63??7.31? on the right). There is a significant difference between two sides, but no significant difference between both sexes.4. The relationship between the inclinational and torsional angles on one hand and the changes in the femoral lengths on the other has been studied by dividing the femurs into groups according to their lengths. It has been shown that the changes in the femoral length exert no effect upon the inclinational and torsional angles of the femoral necks, neither in the male nor in the female.Besides, a comparison has also been made between the femurs of both sides and both sexes, between the femoral lengths and the inclinational and torsional angles, and between the variant ranges of the torsional angles of femurs. A discussion on these problems has been made with reference to the racial differences.

Objective: To investigate the effects and mechanisms of inositol hexaphosphate(IP6)on cell cycle of HT-29 human colon carcinoma cell line. Method: HT-29 cells were exposed to various concentrations of IP6 for certain time. The effect of IP6 on cells proliferation was evaluated by MTT assay. Flow cytometric analysis was performed for cell cycle progression. Immunocytochemical stain was used to detect the expression of p53 and p21 protein. Results: A significant dose-dependent as well as time-dependent growth inhibition was observed in IP6-treated HT-29 cells, and associated with an increase in G1 arrest; Different concentrations of IP6 decreased the abnormal expressions of p53 gene and strongly increased the expression of p21. Conclusions: IP6 had an inhibitory effect on proliferation of HT-29 cells .and its mechanisms might be related to many factors, such as inhibiting the abnormal expression of p53 gene, inducing the expression of cell cycle inhibitor p21 which block the cell cycle.