Objective To study the expressions of matrix metalloproteinase-3 (MMP-3) and osteopontin (OPN) in cervical carcinoma and their relationship with invasion and metastasis. Methods The expression of MMP-3 and OPN were detected in 54 cases of cervical cancer tissues by immunohistochemical SP method. Compared with 15 cases of normal cervical tissues, the relationship was analyzed between both protein expressions and clinical pathology. Results The expression positive rates of MMP-3 and OPN in cervical cancer tissues (70.37 % and 66.7 %, respectively) were significantly higher than those of normal cervical tissues (20 % and 0, respectively), were significantly higher with infiltration depth >1/2 and lymph node metastasis than those with depth≤1/2 and without metastasis , respectively(P <0.05). The expressions of MMP-3 and OPN were related with depth of cervical infiltration and lymph node metastasis of cervical cancer.Expressions between MMP-3 and OPN were significantly positively correlation(r =0.401, P<0.05). Conclusion The expressions of MMP-3 and OPN are correlated with invasion and metastasis of cervical carcinoma.

Background Oxidative stress is one of the pathogenesis mechanisms of diabetic complications.Melatonin is the most powerful antioxidant in living organism.Seldom study on the effects of melatonin on diabetic complications is found in China now.Objective This study aimed to detect the expression and alteration of malonadehyde(MDA),glutathione(GSH)and glial fibrillary acidic protein(GFAP)in retina with experimental diabetes and explore the effects of melatonin on retinal oxidative stress in diabetic rats. Methods The animal models of diabetes were established by injection of 2％ streptozocin via caudal vein(STZ,45 mS/kg)in 48 6-week-old clean SD rats.Isometric citric acid buffer solution wag injected in 24 normal.rats as control group.Seven days after injection of STZ,melatonin(10 mg/kg)was injected into the abdominal cavity of 24 diabetic rats in melatonin group dailv,and the equal volume of normal saline was injected in other 24 diabetic rats(model rats)and 24 rats in normal control group.The animals were sacrificed and retinas were obtained in three groups in 4,6 and 8 weeks respectively.Immunohistochemistry and Western blot were used to detect the expression of GFAP in rat retina.The levels of MDA and GSH in retina homogenate were assayed by spectrophotometer.This experiment complied the Regulations for the Administration of Affair Concerning Experimental Animals by State Science and Technology Commission.Results The expression of GSH in model group was lower than that in melatonin group or control group at 4,6 and 8 weeks (all P<0.05).However,no statistically significant difference was seen in the expression level of GSH between melatonin group and normal control group at 4 and 6 weeks(P>0.05),and in the eighth week,expression of GSH in melatonin group was lower than that in control group(P<0.05).The expressions of MDA and GFAP were more prominent in the model group than in melatonin group and control group in 4,6 and 8 weeks(P<0.05),but no significant difference was found between melatonin group and control group at 4 and 6 weeks(P>0.05).The expressions of MDA and GFAP in melatonin group were higher than those in control group at 8 weeks(P<0.05).Conclusion Melatonin plays a powerful effect in protecting the retina from oxidative stress in diabetes retina by downregulating the MDA level,upregulating GSH level and inhibiting GFAP expression.

Objective To investigate the expression of Survivin gene and the relationship between Survivin and genesis and development of cervical cancer. Methods The expression of Survivin gene in tissues of 40 patients with cervical cancer, 38 patients with cervical hyperplasia and 10 cases of normal cervical were detected by immunohistochemical SP method, and the relationships between Survivin and the clinical pathological factors were analyzed. Results The expression positive rate of Survivin in cervical cancer tissues (60 %) was significantly higher than that in cervical hyperplasia and normal cervical tissues (42.1 % and 0, respectively), and their differences were statistically significant (P < 0.05). The positive expression rate of Survivin was related with histological grade and the clinical stages of cervical cancer.Conclusion Survivin is related to the genesis and development of cervical cancer.

Objective To investigate the scientific research ability and training needs of the new posts of medical undergraduates for better training them.Methods A total of 62 new posts of medical undergraduates from a level-two general hospital in China were recruited by questionnaire survey.Resuits the scientific research ability of the new posts of medical undergraduates is poor,so it is very necessary for their in-service training,93.5~ of them have interest in scientific research and hope to get the training,especially want to take part in the practical activity.Conclusions It is not better that the scientific research ability of the new posts of medical undergraduates,so as early as possible to give the scientific research skill training to them.

Objective To summarize the imaging features of the thoracic canal stenosis caused by ossification of ligamentum flavum, and to evaluate its clinical results of a novel decompression of total laminotomy and autografting in-situ. Methods 31 patients underwent total laminotomy, autografting in-situ and decompression to treat the ossification of thoracic ligamentum flavum. In the study, there were 18 males and 13 females; the average age was 45.7 years (rang, 26 to 73). 3 patients were associated with cervical stenosis, 5 with lumbar stenosis, and 2 with both cervical and lumbar stenosis. 9 were associated with anterior compression by thoracic disc herniation or OPLL. The vertebrae affected were 1 level in 3 patients, 2 levels in 12, 3 levels in 11 and more than 4 levels in 5. 23 affected segments were located at the upper thoracic spine(T1-T4), 19 at the mid-thoracic spine(T5-T8) and 52 at lower-thoracic spine(T9-T12). All the patients underwent total laminotomy and autografting in-situ, 9 patients with thoracic disc herniation or OPLL received discectomy and internal fixation at one stage. The postoperative outcomes were evaluated according to Epstein criteria. Results The patients were followed up 6 to 63 months (mean, 15 months). The clinical outcomes were excellent in 14 cases, good in 7 cases, and fair in 3 cases, and the excellent and good rate was 87.5%. One case experienced neurological complication with Frankel C grade improved to Frankel D grade treated with neurotrophic drug and hyperbaric oxygen and to Frankel E grade at 5 months after operation. 2 presented with lower limb intravenous thrombosis, and CSF leakage happened in 1 case and cured with re-operation. The osteotomy of total laminotomy was bony healed at average of 5.5 months, and there was not new formed ossification anterior to re-implanted vertebral lamina and spinal cord compression. Conclusion MRI combined with CT scanning is a most useful imaging modality for the diagnosis of ossification of thoracic ligamentum flavum. The novel surgical procedure designed by the authors is safe and effective in treating ossification of thoracic ligamentum flavum.

The P53,Rb,nm23,C-berB-2 genes of testicular tissues were assayed with immunohistochemical method in 30 hereditary cryptorchids.The results indicate that nm23 of 4 cases and C-berB-2 of 2 cases were positively stained in 30 cases,and one of the 6 cases was both positively stained in an abdominal undescended testis.The findings suggest that aberrantion of nm23,C-berB-2 genes is associated with testicular neoplasms of retained testis,specifically abdominal undescended testis has trend to variation of correlative oncogenes of testicular tissues.

Objective To prospectively study the minimally invasive hematoma stereotactic aspiration and the recovery of neurological deficits after conservative medical treatment alone, as well as the incidence of post-stroke depression (PSD) in patients with intracerebral hemorrhage, so as to investigate the effect of minimally invasive hernatorna stereotactic aspiration on the recovery of neurological deficits and the incidence of PSD. Methods Fifty-five patients with intracerebral hemorrhage received minimally invasive hematoma stereotactic aspiration (n =25) and conservative medical treatment (n = 30), respectively. The neurological deficits of the patients were assessed by the National Institutes of Health Stroke Scale (NIHSS) at admission, day 14 and 90. The decreased values (all compared to baseline score) of the NIHSS scores were calculated at day 14 and 90, respectively; the modified Rankin Scale (mRS) was used to assess the disabled degree at day 90; the 17-item Hamilton Depression Rating Scale (HAMD) was used to assess the PDS status at day 14 and 90. The correlation between PSD and the degrees of neurological deficit and disability in patients was analyzed. Results The decreased value of the NIHSS score in the stereotactic group at day 14 and 90 was significantly higher than that in the conservative treatment group (all P ＜0. 05), and the value of the mRS score was significantly lover than that in the conservative treatment group at day90 (P＜0. 05). The incidence and the total incidence of PSD in the stereotactic group at day 90 were significantly lover than those in the conservative treatment group (all P ＜0.05). There were significant positive correlation between HAMD and NIHSS scores and HAMD and mRS scores. Conclusions The recovery of neurological deficits was faster after the minimally invasive hematoma stereotactic aspiration. The degree of disability in patients was lower, and the incidence of PSD was also lower than that in the conservative treatment group. PSD was closely correlated with the degrees of neurological deficits and disability.

Objective To investigate the effect of nursing interventions on the quality of life after radiotherapy in nasopharyngeal cancer. Methods 56 patients with nasopharyngeal cancer who received radiotherapy were randomly divided into control group and intervention group. All the patients were given conventional nursing interventions, and those in the intervention group were given accurate evaluation, enhanced psychological guide, reasonable dietary guide, and special nursing for preventing and treating complications. All the patients were evaluated by using quality of life questionnaire core 30 items(QOL-C30). Results The scores of the dimensions of somatic function,psychological function, social function and material life after interventions in the intervention group were significantly higher than those in the control group (P＜0.05 ). Conclusion Nursing interventions can improve the quality of life, and the therapy can be completed smoothly.

It is a trend that clinical researchers overemphasize on hypothesis testing and the P value. However, P value has some limitations in the presentation of study results. Power is a tool for study design and results explanation, especially for negative results. Confidence interval contains more information for clinical results than P value. P value, power, and 95% confidence interval should be provided in the clinical reports to facilitate the interpretation of the results.

Objective To assess the diagnostic value of ChromID for Clostridium Clostridium infection (CDI) on the premise of large sample size,with Meta-analysis.Methods The literature inclusion and exclusion standards were made,and the databases were searched,including PubMed,Cochrane Library,China Biology Medicine disc (CBM),China National Knowledge Infrastructure (CNKI) and so on,using the keywords (ChromID,Clostridium difficile,and chromomgenic agar) along with each of the following words (detection,diagnosis,and infection).Quality assessment of diagnostic accuracy studies (QUADAS) was used to evaluate the quality of the literature.The Meta-disc 1.4 software was used to analyze the data.Results Five studies on 1 851 patients were included in this meta-analysis.The pooled sensitivity was 0.793 (95 ％ CI:0.750 ～ 0.832) for the diagnosis of CDI on 48 h,the pooled specificity was 0.942 (95 ％ CI:0.972 ～0.955),the pooled positive likelihood ratio was 16.817 (95％ CI:7.042 ～ 40.157),with negative hkelihood ratio was 0.124 (95％ CI:0.040 ～0.386) and diagnostic odds ratio for 244.64 (95％ CI:17.711 ～3 379.3).The area under the working curve (AUCSROC) of the subjects was 0.981 4 and the Q* index was 0.939 9.Conclusions Meta-analysis on ChromID for diagnosis of clostridium difficile infection revealedChromID had higher sensitivity,specificity,positive likelihood ratio,and negative likelihood ratio,which can be recommended for clinical use.