BACKGROUND:With elevated medical levels, limb salvage surgery for limb malignant tumor is valuable day by day. At present, the limb salvage surgery has substituted amputation and becomes mainstream and development direction of present surgical treatment of limb tumors. However, so far, there are no unified surgical indications for proximal femur tumor, which are controversial. OBJECTIVE:To investigate the efficacy of artificial total hip arthroplasty combined with extended resection of tumor sections for proximal femoral bone tumors. METHODS:Patients with proximal femoral bone tumors in the Second Xiangya Hospital of Central South University were selected and divided into control group and observation group, with 30 patients in each group. According to the disease, lesion curettage, aneurysm wal inactivation, autologous and (or) al ogeneic bone, mixed bone graft and bone graft fixation were selected to treat the control group. The observation group patients received extended resection of tumor sections and total hip arthroplasty. The operative time, intraoperative blood loss, hospital stay and joint function of two groups were compared. 2 years later, patients were revisited. Metastasis and recurrence rate, death rate and quality of life of two groups were compared. RESULTS AND CONCLUSION:There was no significant difference in intraoperative blood loss and surgery time between the two groups (P>0.05). However, hospital stay of patients in the observation group was shorter than the control group. The excellent and good rate of recovery of joint function (83%) was higher in the observation group than in the control group (53%). The metastasis and recurrence rate within 2 years after surgery (7%) and death rate (3%) were lower in the observation group than in the control group (30%, 23%). Various indicators of quality of life of patients in the observation group were significantly better than the control group (P<0.05). These results confirmed that artificial total hip arthroplasty in the treatment of proximal femur tumors is safe and effective.

Objective Cerebral ischemia would rapidly initiate structural and functional damages in brain, including blood-brain barrier disruption, inflammation, and angiogenesis. The purpose of present study is to investigate the effects of indomethacin, an agent that inhibits cyclooxygenase, on the inflammatory reaction after cerebral ischemia-reperfusion in transient middle cerebral artery occlusion(MCAO)rats and its possible mechanism. Methods Adult male SD rats weighed 250-250g were subjected to either sham surgery or middle cerebral artery occlusion (MCAO) for 2h of brain ischemia and 24h reperfusion. After 24h of reperfusion,the size of cerebral infarction and the neurological deficit were determined by the method of TTC (2,3,5-Triphenyltetrazolium Chloride) staining and Longa's score analysis.The contents of IL-8, IL-1?, TNF-? and MPO in brain tissue were assayed by ELISA. The expressions of ICAM-1 ande E-selectin were evaluated with Western-blot. Results It was observed that indomethacin (6 or 9mg/kg i.p pretreatment for 5d, once a day) significantly reduced cerebral infarct volume and ameliorated the neurological deficit (P

Objective:? N oxalo L ?,? diaminopropionic acid were isolated from the powder of red ginseng. its physiological characteristics were studied. Method:This material is obtained by CM Sephadex C 25 and FPLC MonoQ. We have examined the effect of ? N oxalo L ?,? diaminopropionic acid on contraction of artery of guinea pig. Result:When histamin and adrenalin do not exist, there is neither contraction nor relaxation. It can strengthen induction of contraction of artery of guinea pig, but it has no such effect on adrenalin. Besides, it doesn't induce plaque coagulation through adrenalin, ADP blood coagulation enzyme and collagen. It doesn't suppress the transformation from tension peptide of blood vessel to enzyme.Conclusion:Hemostatic effect of ? N oxalo L ?,? dlaminopropinomie acid may caused by vesseles contraction through promoting Histamine.

Objective To investigate the expression and biological significance of microRNA-126 in colon cancer tissue.Methods The expression of microRNA-126 in colon cancer tissues and adjacent tissues of colon cancer patients was detected by real-time quantitative polymerase chain reaction (RT-PCR) in 104 patients with colon cancer surgery (total of 104 pairs).The lentiviral vector was used to construct microRNA-126 cell line,and the effects of microRNA-126 on proliferation,migration and invasion of colon cancer cells were further studied in vitro.Results The relative expression of microRNA-126 in colon cancer tissues (0.63±0.11) was significantly lower than that in adjacent tissues (1.08±0.15),the difference was statistically significant(t=14.561,P＜0.01).The positive rate of microRNA-126 expression in colon cancer tissues (61.5％) was significantly lower than that in adjacent cancer tissues (86.5 ％) the difference was statistically significant(x2=16.908,P＜0.05).The expression of microRNA-126 was significantly correlated with Dukes stage,depth of invasion,lymph node metastasis and distant metastasis (P＜0.05).In the Transwell experiment with matrix glue,the number of cell migration in transfection group (11.26±4.85) was significantly lower than that in blank control group (264.37±32.15),the difference was statistically significant (t=23.418,P＜0.01).In the Transwell experiment without matrix glue,the number of cell migration in transfection group (83.75 ± 13.74) was significantly lower than that in blank control group (339.64 ± 26.38),the difference was statistically significant(t=12.682,P＜0.01).MicroRNA-126 could inhibit the proliferation and invasion of SW480 cells.Conclusion MicroRNA-126 can significantly inhibit the development of colon cancer cells and affect the biological behavior of colon cancer cells.

, which will provide enough seed cells for peripheral nerve tissus engineering research.

Objective:To study the effects of different duty cycles [modulated by pulse repetition frequency(PRF)] of diagnostic ultrasound on enhancing blood perfusion in hypovascular tumors.Methods:Walker-256 tumor cells were injected subcutaneously into the inner thigh of 15 SD rats, and the rats were randomly divided into 3 groups according to PRF: group A(PRF=50 Hz), group B(PRF=1.0 kHz) and group C(PRF=2.0 kHz), with 5 rats in each group. The tumor-bearing rats in each group were treated with ultrasound combined with microbubbles for 10 minutes. Contrast-enhanced ultrasound was performed before treatment, immediately after treatment, and 4 hours after treatment, respectively, to compare the peak intensity(PI), area under curve(AUC), and tumor perfusion area in each group.Results:Immediately after treatment, PI and AUC values of group A and B increased compared with those before treatment (all P<0.05); 4 hours after treatment, PI and AUC values of group B continued to increase, superior to that immediately after treatment (all P<0.05), while PI and AUC values of group A and C were decreased compared with those immediately after treatment (all P>0.05). Immediately after treatment, the tumor blood perfusion area in group A and B increased significantly compared with that before treatment (all P<0.05); 4 hours after treatment, the tumor blood flow perfusion area of group B continued to increase, which was better than that immediately after treatment ( P<0.05), and the tumor blood perfusion areas of group A and C decreased compared with that immediately after treatment (all P>0.05). Conclusions:Low-intensity diagnostic ultrasound stimulated microbubble cavitation with PRF=1.0 kHz can enhance tumor blood flow significantly which can last for more than 4 hours.

Aged ; Humans ; Male ; Pharyngeal Neoplasms ; Polyps ; Pyriform Sinus ; pathology

ObJective To explore the value of acute physiology and chronic health evaluation Ⅲ(APACHE Ⅲ ) score and the concentration of serum cholinesterase (ChE) in predicting the condition and prognosis of patients with severe acute pancreatitis (SAP) within 24 hours afar hospitalization.Methods Sixty-two SAP patients were enrolled and APACHE Ⅲ score was assessed and the concentration of serum ChE was detected within 24 hours after hospitalization.The correlation between the concentration of serum ChE,APACHE Ⅲ score and the condition and prognosis was analyzed.Results There were 44 survivalcases and 18 dead cases.The APACHE Ⅲ score of the surval patients was significantly lower than that of the dead patients [(52.16 ± 13.76) scores vs.(97.10 ± 15.85) scores] (P＜0.01).The concentration of serum ChE of survival patients was significantly higher than that of the dead patients [ (3685 ± 466) U/L vs.(2109 ± 345) U/L] (P＜ 0.01 ).The higher APACHE Ⅲ score was,the lower the serum ChE concentration was,and the higher the mortality rate was.APACHE Ⅲ score and the concontration of serum ChE both had statistical significances compared with the prognosis in the Logistic regression analysis (P =0.0043,0.0075);APACHE Ⅲ score (95％ CI 1.0306-1.1507),the concentration of serum ChE (95％ CI0.9986-1.0125 ).ROC areas under curve (AUC) of APACHE Ⅲ score,serum ChE concentration with the prognosis were 0.936 and 0.882,respectively.There was no significant difference (P=0.0820).In combined prediction of APACHE Ⅲ score and serum ChE concentration,AUC was 0.952,and its predicting accuracy was higher than either APACHE Ⅲ score or serum ChE concentration (P=0.0016,0.0027).Conclusions APACHE Ⅲ score and the concentration of serum ChE both are significantly correlated with the condition and prognosis of SAP patients.Their combined detection can significantly improve the accuracy of prognosis judgement and provide some clinical guidances for treatment.

BACKGROUND:Resin infiltration is a novel approach in treating non-cavitated caries lesions on smooth surfaces, and the effectiveness comparison between resin infiltration and remineralizing therapy is required.
OBJECTIVE:To compare the effects of resin infiltration and remineralizing therapy on inhibition of non-cavitated lesions in vitro.
METHODS:Three subsurface lesions were created on 35 bovine labial specimens. One of the lesions was permeated with Icon? infiltrant, one was applied by 0.1%NaF solution daily for 7 consecutive days, whereas one lesion remained as the untreated control. Subsequently, half of each specimen was covered with nail varnish
(baseline) and the other half was re-exposed to a demineralizing solution for 5 days (experimental). The specimens were cut perpendicularly to the surface, stained with Rhodamine B and observed with fluorescence microscope.
RESULTS AND CONCLUSION:For lesions permeated with Icon? infiltrant and applied by 0.1%NaF solution, the progression of lesion depth was significantly decreased (P<0.05) compared with the untreated control. Lesions permeated with Icon? infiltrant got more significantly reduced lesion progression (P<0.05) compared with the ones applied by 0.1%NaF solution. It can be concluded that both resin infiltration and remineralizing therapy have active effects on inhibition of non-cavitated lesions, and fil ing the pores with Icon? infiltrant can inhibit further demineralization even better.

Farnesoid X receptor (FXR) belongs to the nuclear receptor superfamily. It is highly related to the formation of metabolic syndrome and the glucose homeostasis, and therefore represents an important drug target against metabolic diseases and diabetes. In recent years, great progress has been made in the agonists, antagonists, and crystal structures of FXR. The diverse FXR ligands and their structure-activity relationship are reviewed in this article. The advances in the crystal structures of FXR in complex with different ligands are also introduced.