1.Screening of miRNAs in ovarian cancer patients and its correlation with clinicopathological features
Tao ZHU ; Ping ZHANG ; Wei ZHENG
China Oncology 2016;26(3):201-207
Background and purpose:The prognosis of ovarian cancer is poor and the diagnosis is relatively late. It is needed to search for early diagnosis and treatment for ovarian cancer. This study investigated the expression of serum miRNAs in patients with malignancy or benign ovarian tumor preoperativey and analyzed its correlation with clinicopathological progress and prognostic features of epithelial ovarian cancer.Methods:Forty-eight miRNAs which have been reported to be related to ovarian cancer were ordered. The differential expression of 48 miRNAs in the serum of patients with malignant or benign epithelial ovarian tumors was detected by TaqMan low density array. The differentially expressed miRNAs were further confirmed by real-time fluorescent quantitative polymerase chain reaction (RTFQ-PCR). The relationship between the expression level of selected miRNA and clinical clinicopathological factors, progress and prognosis were analyzed.Results:TaqMan low density array and further RTFQ-PCR showed that only miR-125b was sig-nificantly increased in 135 ovarian cancer patients as compared with 38 individuals with benign tumor. The expression of miR-125b was higher in early stage patients than that in advanced stage patients (P=0.039). The patients without residual tumor expressed more miR-125b than patients with residual tumor (P=0.013). The high level of miR-125b was signifi-cantly correlated with longer progress free survival (PFS) (P=0.003), but not correlated with overall survival (OS) (P=0.069). Conclusion:MiR-125b may play an important role in the pathogenesis of epithelial ovarian cancer and prognosis. It may be a potential gene to predict the recurrence of epithelial ovarian cancer, but the change of gene expression at different stages and its underlying mechanism need further research.
2.A TECHNIQUE FOR DETECTION OF PLANT VIRUSES ──TISSUE BLOTTING
Mingquan XU ; Ping ZHENG ; Rongwei LIU ; Qing LIU ; Tao WANG
Microbiology 1992;0(05):-
Tissue blotting, based on Enzyme-Linked Immunosorbent Assay (ELISA), is a technique for detection of plant viruses. This technique is not only high sensitivity and specificity, but also simpler and more rapid for detection. Samples that are blotted on membrane can be kept over three months. The results can directly display the section of virus infected. It is especially suitable for detection of plant viruses on a large scale.
3.Advances in the Research on Microbial Phytases
Ping-Ping YANG ; Zheng-Hong XU ; Yan WANG ; Wen-Yi TAO ;
Microbiology 1992;0(03):-
Phytases are studied widely in plants and microorganisms. Interest in these enzymes has been stimulated by the fact that phytase has the advantage in forage, food process and medicine. This paper reviews recent trends on the production, purification, properties and gene of microbial phytases.
4.Effect of Fuzheng Huayu recipe on CYP450 isozymes in normal and liver fibrosis rats.
Tian-hui ZHENG ; Wei LIU ; Shu-ping LI ; Tao YANG ; Chang-hong WANG ; Cheng-hai LIU
China Journal of Chinese Materia Medica 2015;40(6):1166-1172
To study the effect of Fuzheng Huayu recipe (FZHY) on five types of isozymes of cytochrome P450 (CYP450) of normal and liver fibrosis rats by using the cocktail probe method. Dimethylnitrosamine ( DMN) was injected to induce the liver fibrosis model. After the tail vein injection with Cocktail probe solutions prepared with five CYP450s probe substrates (phenacetin-CYP1A2, omeprazole-CYP2C9, tolbutamide-CYP2C19, dextromethorphan-CYP2D6, midazolam-CYP3A4), the plasma concentrations of the five probe substrates were determined by LC-MS/MS, and the pharmacokinetic parameters were calculated by PK solutions 2. After the oral administration with FZHY, normal rats given phenacetin, omeprazole, tolbutamide and dextromethorphan showed increase in AUC(0-t) and decrease in CL to varying degrees, indicating that FZHY obviously inhibited the activities of CYP1A2, CYP2C9, CYP2C19 and CYP2D6 in normal rats, but with no obvious effect on the activity of CYP3A4. After the oral administration with FZHY, liver fibrosis rats treated with CYP2C9 showed the significant increase in AUC(0-t) and significant decrease in Vd, hut with no obvious changes in the pharmacokinetic parameters of other four types of prove substances, suggesting that FZHY could significantly inhibit the activity of CYP2C9 in rats but had no effect on the activities of CYP1A2, CYP2C19, CYP2D6 and CYP3A4. The changes in the activity of CYP450 isozymes in liver fibrosis rats may be the reason for FZHY's different effects on CYP450 isozymes in normal and liver fibrosis rats.
Animals
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Cytochrome P-450 Enzyme System
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genetics
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metabolism
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Disease Models, Animal
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Drugs, Chinese Herbal
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administration & dosage
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chemistry
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pharmacokinetics
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Humans
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Isoenzymes
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genetics
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metabolism
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Liver Cirrhosis
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drug therapy
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enzymology
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genetics
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Male
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Mass Spectrometry
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Rats
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Rats, Wistar
5.Determination of scopolin, chlorogenic acid, scopoletin, isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C in plants of Erycibe.
Xiao-kun XU ; Zhi-yong CHEN ; Li-ping LIAO ; Zi-jia ZHANG ; Zheng-tao WANG
China Journal of Chinese Materia Medica 2015;40(6):1119-1122
OBJECTIVEAn accurate and reliable analytical method for-simultaneous determination of six active components (scopolin, chlorogenic acid, scopoletin, isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C) in plants of Erycibe was developed.
METHODScopolin, chlorogenic acid, scopoletin, isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C in the samples were well separated in analytical HPLC by gradual elution with methanol-0.1% formic acid solution. The chromatographic condictions: Agilent Poroshell 120 EC-C18 column, flowing rate being 1 mL x min(-1), detecting wavelength at 345 nm.
RESULTGood linearities of scopolin, chlorogenic acid, scopoletin, isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C were in the range of 0.026 8-2.68, 0.027 0-2.70, 0.008 1-0.81, 0.018 8-1.88, 0.017 6-1.76, 0.019 6-1.96 μg, respectively (r > 0.999 6). The average recoveries of the six components were 98.1%, 98.7%, 100.8%, 100.4%, 99.7%, 101.1%; the relative standard deviations were 2.67%, 2.86%, 2.62%, 1.98%, 2.76%, 2.19%.
CONCLUSIONThe method is simple, feasible and reproducible and can be used for the quality control of plants of Erycibe.
China ; Chlorogenic Acid ; analogs & derivatives ; analysis ; Chromatography, High Pressure Liquid ; methods ; Convolvulaceae ; chemistry ; Coumarins ; analysis ; Drugs, Chinese Herbal ; analysis ; Glucosides ; analysis ; Scopoletin ; analysis
6.Application of Normal Phase Liquid Chromatography-Evaporative Light-Scattering Detection for Determination of Paraffin Wax in Food
Xuan SHENG ; Yanyun HU ; Lei ZHANG ; Hao SUN ; Ping ZHENG ; Farong TAO ; Yanyan YANG ; Ji HANG
Chinese Journal of Analytical Chemistry 2009;37(12):1765-1770
A method was proposed for the separation and determination of paraffin waxes in food by HPLC-evaporative light scattering detection (ELSD). A normal-phase column was used to separate nonparaffinic and paraffinic materials without resolving the latter into individual components. The t-test method was adopted for the evaluation of mean difference between response factors of n-alkanes in paraffin waxes on ELSD detector. No mean difference was obtained between response factors, which can be used for quantitative determination of paraffin waxes in food. The determination results obtained by HPLC-ELSD were compared with those by GC-MS. The linear range for the determination of paraffin waxes was in the range from 10 to 500 mg/L with a correlation coefficient of 0.9988, and the limit of detection was 1.0 mg/L. With the spiking level of 10, 50 and 100 mg/kg, the recovery ranged from 84.6% to 105.4% and the relative standard deviation ranging from 5.4% to 7.2%. The proposed method is simple, fast and sensitive.
7.Effects of Chinese herbal medicine Jiangzhi Granule on expressions of liver X receptor α and sterol regulatory element-binding protein-1c in a rat model of non-alcoholic fatty liver disease.
Lili YANG ; Miao WANG ; Tao LIU ; Haiyan SONG ; Dongfei LI ; Peiyong ZHENG ; Ping LIU ; Guang JI
Journal of Integrative Medicine 2011;9(9):998-1004
To study the effects of Jiangzhi Granule (JZG), a compound traditional Chinese herbal medicine, in regulating liver X receptor α (LXRα) and sterol regulatory element-binding protein-1c (SREBP-1c) expressions in a rat model of non-alcoholic fatty liver disease (NAFLD).
8.Screening of early prediction ultrasonic index for prognosis of severe hepatitis and its value evaluation
Lili WU ; Jiayi LIANG ; Zhongzhen SU ; Tao WU ; Ping WANG ; Rongqin ZHENG
Chinese Journal of Ultrasonography 2014;23(7):602-604
Objective To select early prediction indexes related to the prognosis of severe hepatitis in ultrasonography,and evaluate its clinical value.Methods Clinical data of 269 cases of severe hepatitis patients was analyzed retrospectively.Cox proportional hazards regression model was adopted to analyze 13 possible prediction ultrasonic parameters for severe hepatitis,including thickness of the right hepatic lobe,portal vein (PV) diameter,volume of ascites,etc.After selected from these parameters,the clinical value of early prediction factor was evaluated with ROC curve.Results Multivariate analysis showed that thickness of the right hepatic lobe,portal vein (PV) diameter,opening of paraumbilical vein and bile viscosity were related to the death of severe hepatitis patients (P < 0.05).The thickness of the right hepatic lobe contributed most (Wald =40.479),its sensitivity and specificity for prediction were 93.4%,82.8% respectively(P < 0.01).Conclusions Thickness of the right hepatic lobe,PV diameter,opening paraumbilical vein and bile viscosity are early prediction factors for severe hepatitis,need to be monitored in treatment,especially for thickness of the right hepatic lobe.
9.Effects of the mixture of Swertia pseudochinensis Hara and Silybum marianum Gaertn extracts on CCl4-induced liver injury in rats with non-alcoholic fatty liver disease.
Zhimin MAO ; Haiyan SONG ; Lili YANG ; Tao LIU ; Dongfei LI ; Peiyong ZHENG ; Ping LIU ; Guang JI
Journal of Integrative Medicine 2012;10(2):193-9
To study the mechanism of liver injury induced by carbon tetrachloride (CCl4) in rats with non-alcoholic fatty liver disease (NAFLD), and the therapeutic effects of the extract mixture of Dangyao (Swertia pseudochinensis Hara) and Shuifeiji (Silybum marianum Gaertn) on NAFLD rats with liver injury.
10.DEEP FERMENTATION TECHNIQUE OF TRICHOLOMA MATSUTAKE MYCELIUM
Ping LIU ; Wen-Yi TAO ; Zheng-Hong XUE ; Zhong-Hua AO ; Zhi-Hao SUN ;
Microbiology 1992;0(05):-
The deep fermentation technique of Tricholoma matsutake is systemically studied in this paper firstly. The best culture determined by orthogonal test is 3g/L of cornflour, 1g/L of glucose, 1g/L of bean cake flour, 1mL/L of corn steep liquid, 1g/L of KH 2PO 4. The best fermenting condition is: 25℃, rotating speed 160 r/min, pH5.0,inoculating amount 10%, 120mL culture medium per 500mL flask. Under these conditions, the mycelia reach 12.94g/L after fermenting 12d.