1. Effects of geldanamycin on cell proliferation and apoptosis of human ovarian cancer cell line SKOV3 and their mechanism
Tumor 2011;31(10):899-905
Objective: To investigate the cytotoxic effects of geldanamycin on human ovarian cancer SKOV3 cells, and to explore the possible mechanisms of apoptosis and cell proliferation inhibition of SKOV3 cells induced by geldanamycin. Methods: MTT method was performed to evaluate the inhibitory effects of geldanamycin alone or in combination with cisplatin on proliferation of SKOV3 cells. Cell cycle and apoptosis rate of SKOV3 cells treated with geldanamycin were detected by flow cytometry. The expressions of Akt and Raf-1 proteins were examined by immunocytochemistry and flow cytometry. Results: The proliferation of SKOV3 cells was inhibited by different concentrations of geldanamycin in a time- and dose-dependent manners (P<0.05). Different concentrations of geldanamycin strengthened the inhibitory effect of cisplatin on proliferation of SKOV3 cells, and this inhibitory effect was strengthened with the increased concentration of geldanamycin within a certain concentration range. The apoptosis rate of SKOV3 cells was raised up gradually after treatment with 0-2000 nmol/L geldanamycin for 48 h, and the number of cells in G2/M phase was increased significantly (P<0.05). The results of immunocytochemistry and flow cytometry showed that the expressions of Akt and Raf-1 proteins in SKOV3 cells induced by geldanamycin were significantly down-regulated in a dose-dependent manner (P<0.05). Conclusion: Geldanamycin can inhibit the cell proliferation and induce the apoptosis of human ovarian cancer SKOV3 cells in vitro , which may be associated with downregulation of the expressions of Akt and Raf-1 proteins. Copyright© 2011 by TUMOR.
2. Inhibitory effects of manumycin combined with cisplatin on the proliferation of human ovarian cancer cell line 3AO in vitro and in vivo
Tumor 2011;31(3):210-216
Objective: To investigate the inhibition effects of manumycin combined with cisplatin (DDP) on the proliferation of human ovarian cancer cell line 3AO and the growth of transplantation tumors in nude mice, and to analyze its possible mechanisms. Methods: MTT colorimetric assay was performed to evaluate the cytotoxic effect of manumycin combined with DDP on 3AO cells. The model of nude mice bearing ovarian transplantation tumor was established. The expressions of survivin, NF-κB, vascular endothelial growth factor (VEGF) and caspase-3 proteins were detected by immunohistochemical method. The cell cycle distribution and apoptosis rate were examined by flow cytometry. Results: The proliferation of 3AO cells was inhibited by manumycin in a time- and dose-dependent manner (P <0.05). The manumycin of 10, 20, 40 or 60 μmol/L could strengthen the cytotoxic effect of DDP on 3AO cells. The tumor size in nude mice of each drug-treated group was significantly smaller than that of the control (untreated) group (P <0.05). Compared with the DDP alone and the manumycin alone group, the expressions of survivin, NF-κB and VEGF proteins were significantly down-regulated while the expression of caspase-3 protein was significantly up-regulated (P <0.05) in the combination (manumycin plus DDP) group (P <0.05). The apoptosis rates were increased gradually in the control group, manumycin group, DDP group and the combination group. In each drug-treated group, the number of cells in G0/ G 1 phase was decreased significantly, while which in G2/M phase was increased significantly (P <0.05), as compared with the control group. The number of cells in S phase did not show any changes in each group. Conclusion: The combination of manumycin and DDP shows an enhanced ability to inhibit the proliferation of human ovarian cancer cells 3AO and increase their apoptosis. This effect may be associated with the down-regulation of survivin, NF-
3.Dynamic analysis of anti-Cov antibody and SARS coronavirus in the plasma of 25 SARS patients
Dongping XU ; Zheng ZHANG ; Yuanli MAO
Medical Journal of Chinese People's Liberation Army 1983;0(05):-
Objective To observe dynamic responsive regularity of specific IgM and IgG antibodies in SARS patients. Methods 145 specimens of plasma from 25 cases of clinically diagnosed SARS patients were examined in the study. ELISA was employed to detect IgM and IgG antibodies against SARS coronaviral antigens. Nested RT-PCR was used to qualitatively determine SARS coronavirus (SARS-CoV). Results 49.0% (71/145) and 54.5% (79/145) of the samples were positive for IgM and IgG antibodies, respectively. Both antibodies were found to be detected in 84.0% of the patients. The antibodies were found to be detectable from the 2nd to 4th week after the onset of disease in most patients, and there was a fendeney of sising in positive rate until the 5th week after the onset. Thereafter, the detectable rate of IgM antibody began to decline, while that of IgG antibody remained to rise. Positive rate for serum SARS-CoV was 15.8% (18/114) for all samples or 40.0% (10/25) of patients. Most virus-positive samples were those which were collected within 4 weeks after disease onset. Conclusions Anti-SARS-CoV IgM/IgG antibody and detection of virus in plasma could serve as practical diagnostic indicators for SARS. In most cases, when the serum was pasitive for antibody the serum virus positive rate would soon declined. However, concomitant existence of antibody and viral sequence in plasma was observed in a few patients.
4.Minimally Invasive Percutaneous Nephrolithotomy for the Treatment of Impacted Upper Ureteral Calculi: A Report of 49 Cases
Ziqiang DONG ; Zheng MAO ; Ping ZHANG
Chinese Journal of Minimally Invasive Surgery 2005;0(10):-
Objective To investigate the efficacy of minimally invasive percutaneous nephrolithotomy(MPCNL) for impacted upper ureteric calculi.Methods Retro-urethral catheterization was conducted in the diseased ureter under cyctoscope.After a channel from the skin surface into the middle calyx was established under the guidance of X-ray or B ultrasonography,pneumatic lithotripsy was conducted to break up stones under ureteroscopy.Results The success rate of pneumatic lithotripsy was 100%.The rate of postoperative macroscopic hematuria was 55.1%(27/49).The symptom lasted one to three days in 26 cases,and 1 case had apparent hematuria with blood clot flowing out from nephrostomy tube,which lasted 8 days.10.2%(5/49) of cases was complicated with postoperative pyrexia.The stone-free rate in one week and one month postoperatively was 93.9%(46/49) 98.0%(48/49),respectively.Follow-up observations in 49 cases for 1-12 months(mean,5 months) showed no major complications.Conclusions MPCNL for impacted upper ureteral calculi has the advantages of simple performance,less complications and satisfactory efficacy.
5.Analysis of surgical transurethral treatment for small-size benign prostatic hyperplasia
Ziqiang DONG ; Kejun LI ; Ping ZHANG ; Lusheng ZHANG ; Zheng MAO
Chinese Journal of Postgraduates of Medicine 2006;0(32):-
0.05), but there were significant differences after the operation (P
6.Experimental study of norcantharidins effects on proliferative growth and apoptosis of human cholangiocarcinoma cell line QBC939
Jiangliang YUAN ; Jian ZHENG ; Guanghua MAO ; Zining QI ; Shuangwei ZHANG
Cancer Research and Clinic 2011;23(11):768-771
Objective To study the growth effect of human cholangiocarcinoma cell line QBC939 treated by norcantharidin (NCTD) and preliminary illustrate the potential mechanism.Methods The human cholangiocarcinoma cell line QBC939 was detected by MTT assay,flow cytometry,immunocytochemistry after the treatment of NCTD in vitro.Results NCTD displayed inhibitory effect on growth of QBC939 from different doses of 0.125,0.75,2.5,10,120 μg/ml after 48 h (P <0.05).It was in a dose and time dependent manner.Dose-effect curve was drawn and IC50 value was (3.66±1.14) μg/ml.The flow cytometric profiles showed that the rate of cell apoptosis enhanced following increasing the concentration of NCTD[(8.6±0.4) %,(17.6±0.3) %,(22.9±0.4) %,(25.5±0.9) % and (31.1±1.5) %,respectively]and cells blocked in the G2/M phase after treatment with 2.5 μg/ml NCTD[(14.1±1.0) % and (5.7±0.3) %].The expression of the protein caspase-3 elevated after different concentrations of NCTD co-cultured with QBC939 compare with contrast group.Conclusion NCTD has an inhibitory effect on proliferation of QBC939 cell line,and the mechanism might be related to the induction of cell apoptosis and blockade of cell cycle.
7.Ultrasonographic characteristics of biliary stricture after orthotopic liver transplantation
Yongjiang MAO ; Rongqin ZHENG ; Jie REN ; Dongmei HUANG ; Bo ZHANG
Chinese Journal of Ultrasonography 2008;17(12):1045-1047
Objective To evaluate the charateristics of biliary stricture after orthotopic liver transplantation(OLT) by ultrasound. Methods Diametes,thickness,characteristic echo of bile were observed by ultrasound in 41 patients with biliary stricture after OLT,and the results were compared with those of 46 patients without biliary complication after OLT.Results The diameter of intrahepatic bile,thickness of bile,incidence of intrahepatic bile dilation or hyperechogenicity in biliary stricture patiens were higher than those in patients without biliary complication,there were significant differences between two groups(P<0.05).Conclusions The series of ultrasonographic character such as bile dilation,thickeness or hyperechogenicity of biliary wall,thin of bile duct are available to diagnose biliary stricture.
8.Study on scientific misconduct Clinical Scientific Research and its Countermeasures
Fuhao ZHENG ; Lu ZHANG ; Mao CHEN ; Qi LI ; Gang CHEN
Chinese Journal of Medical Science Research Management 2016;29(1):17-20
This article analyzed the types and causes of clinical research misconduct including the definition and background.We then set up the research management system to control and prevent such misconduct behavior.Our strategy includes using various technical tools to actively carry out clinical research integrity related education and training program,establishing integrated control model,and clinical research misconduct handling mechanism to jointly cope with the problems,to promote a good atmosphere for clinical research and to establish the integrity of the research.Our goal is to promote the medical technology development and to improve the patient care.
9.A review of validation for nursing competency models
Xin WANG ; Songqin GAO ; Zheng ZHANG ; Yanjun MAO
Chinese Journal of Practical Nursing 2016;32(15):1192-1196
Specialization development of nursing has increasing demand for nursing staff. Competency model is a professional way for the person-post matching, which may replace the job analysis as a new research direction in nursing human resource management. In this paper, the validation and application of nursing competency model were reviewed from the perspectives of competency model, to discuss the thought and method of verifying competency model, so as to lay the foundation for the clinical application of nursing competency model, and promote the rapid development of nursing.
10.Nursing cooperation for patients with severe aortic stenosis receiving transcatheter aortic valve replacement using balloon-expandable aortic valve stent
Zheng ZHANG ; Songqin GAO ; Xin WANG ; Yanjun MAO
Journal of Interventional Radiology 2014;23(10):926-928
Objective To discuss the nursing cooperation for patients with severe aortic stenosis who are receiving transcatheter aortic valve replacement using balloon-expandable aortic valve stent. Methods Percutaneous interventional transcatheter aortic valve replacement was employed in 9 patients with severe aortic stenosis. Active preoperative preparation, sufficient psychological care, close observation of vital signs and effective surgery cooperation during the operation were carried out. The clinical results were analyzed. Results Successful operation was accomplished in all the nine patients. After the surgery, all patients were in good condition, and no nursing-related complications occurred. Conclusion Correct understanding of the cooperation with the procedure of transcatheter aortic valve replacement using balloon-expandable aortic valve stent, excellent preoperative psychological care and active preoperative preparation, etc. are the most important things to ensure a successful surgery.